Abstract:
:5'AMP nucleotidase activity was localized by electron microscopy in Dictyostelium discoideum during cell differentiation. In addition, the activity was assayed by micro enzymic methods in sections dissected from specific cellular regions of lyophilized individuals. The results of the 2 procedures were in agreement, demonstrating that at the culmination stage of development the activity is strikingly localized in the prestalk cells adjacent to the prespore region. The cytochemically stained reaction product appeared only along the plasma membrane of the cells. As prestalk cells migrate into the stalk sheath and undergo differentiation, the activity is rapidly lost. Examination of stained cells at high magnification revealed the product accumulation to be primarily at the cell surface, suggesting that the enzyme functions extracellularly. Occasionally, cells having the morphological appearance of prestalk cells were found within the prespore region. These cells demonstrated 5'AMP nucleotidase activity at their plasma membrane in sharp contrast with neighbouring prespore cells. The strategic localization of 5'AMP nucleotidase may reflect a mechanism for establishing and maintaining regulatory levels of extracellular 5'AMP and/or adenosine during pattern formation in this model system.
journal_name
J Cell Scijournal_title
Journal of cell scienceauthors
Armant DR,Stetler DA,Rutherford CLsubject
Has Abstractpub_date
1980-10-01 00:00:00pages
119-29eissn
0021-9533issn
1477-9137journal_volume
45pub_type
杂志文章abstract::The human Ras superfamily of small GTPases controls essential cellular processes such as gene expression and cell proliferation. As their deregulation is widely associated with human cancer, small GTPases and their regulatory proteins have become increasingly attractive for the development of novel therapeutics. Class...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.210419
更新日期:2018-01-08 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.195255
更新日期:2016-11-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.191379
更新日期:2016-08-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.179788
更新日期:2016-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2002-04-01 00:00:00
abstract::Previous work from this laboratory has indicated that very low concentrations of vinblastine block HeLa cells at mitosis in the presence of a full complement of microtubules and without major disruption of spindle organization. In the present study we analyzed the structural organization of mitotic spindle microtubule...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::We have used MPM-2, a monoclonal antibody raised against mitotic HeLa cells, to stain a Drosophila cell line, whole mounts of Drosophila embryos, and sectioned tissue from embryonic and larval stages of development. MPM-2 recognizes a major phosphoprotein of approximately 125 X 10(3) Mr in Drosophila tissue culture ce...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:1987-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2009-01-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract::In a wild population of the American newt Notophthalmus viridescens 15 females out of a total of 94 were found to be heterozygous for a paracentric inversion which includes almost the whole of the longer arm of the smallest chromosome (XI). The inversion was recognized in preparations of lampbrush chromosomes because ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1977-04-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.187419
更新日期:2017-01-01 00:00:00
abstract::The neuromuscular junction (NMJ) is the largest, most-complex synapse in the human body. Motor neuron (MN) diseases, such as amyotrophic lateral sclerosis (ALS), specifically target MNs and the NMJs. However, little is known about the reasons for MN-selective neuronal and synaptic vulnerability in MN diseases. Here, u...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.234492
更新日期:2019-12-02 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.02349
更新日期:2005-05-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.137604
更新日期:2014-02-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.087700
更新日期:2011-08-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
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更新日期:2018-08-03 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.01302
更新日期:2004-08-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章,评审
doi:10.1242/jcs.051326
更新日期:2010-02-15 00:00:00
abstract::In vitro experimental evidences suggest that the proteolytic degradation of the extracellular matrix (ECM) by activation of the urokinase-type plasminogen activator (uPA)/plasmin system may affect growth factor activity and bioavailability. However, no direct in vivo observations were available to support this hypothe...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Different isoforms of non-muscle myosin II have different distributions in vivo, even within individual cells. In order to understand how these different distributions arise, the distribution and dynamics of non-muscle myosins IIA and myosin IIB were examined in cultured cells using immunofluorescence staining and tim...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::Triosephosphate isomerase (TPI) is a glycolytic enzyme that converts dihydroxyacetone phosphate (DHAP) into glyceraldehyde 3-phosphate (GAP). Glycolytic enzyme dysfunction leads to metabolic diseases collectively known as glycolytic enzymopathies. Of these enzymopathies, TPI deficiency is unique in the severity of neu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.124586
更新日期:2013-07-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1986-12-01 00:00:00
abstract::Postsynaptic density protein 95 (PSD-95/SAP-90) is a palmitoylated membrane-associated guanylate kinase that oligomerizes and clusters ion channels and associated signaling machinery at excitatory synapses in brain. However, the mechanism for PSD-95 oligomerization and its relationship to ion channel clustering remain...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00617
更新日期:2003-08-01 00:00:00
abstract::We have isolated suppressors of the deletion allele of delta-tubulin, uni3-1, in the biflagellate green alga Chlamydomonas reinhardtii. The deletion of delta-tubulin produces cells that assemble zero, one or two flagella and have basal bodies composed primarily of doublet rather than triplet microtubules. Flagellar nu...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.00859
更新日期:2004-01-15 00:00:00
abstract::Nerve branching is controlled by intrinsic and extrinsic cues, one of which may be a small applied electric field. Lateral processes were induced by passing current through a micropipette placed at 90 degrees to the shaft of a developing nerve. The appearance of processes was a polarised event with a large majority ar...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Wnts are glycan- and lipid-modified morphogens that are important for cellular responses, but how Wnts are secreted in polarized epithelial cells remains unclear. Although Wntless (Wls) has been shown to interact with Wnts and support their secretion, the role of Wls in the sorting of Wnts to the final destination in ...
journal_title:Journal of cell science
pub_type: 杂志文章
doi:10.1242/jcs.126052
更新日期:2013-07-01 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract::Rac1 is a small Rho family GTPase that regulates changes in cell morphology associated with cell spreading and migration. Integrin-mediated adhesion is known to activate Rac1 and to regulate the interaction of Rac1 with downstream effectors. Currently, it is not clear how integrins signal Rac1 activation following cel...
journal_title:Journal of cell science
pub_type: 杂志文章
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更新日期:2002-11-15 00:00:00
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journal_title:Journal of cell science
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00