解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Escherichia coli is the most common cause of human and canine urinary tract infection (UTI). Clonal groups, often with high levels of antimicrobial resistance, are a major component of the E. coli population that causes human UTI. While little is known about the population structure of E. coli that causes UTI in dogs,...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.00788-18
更新日期:2018-08-27 00:00:00
abstract::The incidence of candidemia caused by Candida albicans and Candida glabrata is constantly increasing and is accompanied by the rising use of the few available antifungals. The widespread use of echinocandins and azoles for the treatment of invasive candidemia has enhanced the development of antifungal resistance, resu...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00420-18
更新日期:2018-08-27 00:00:00
abstract::Bovine tuberculosis (bTB) caused by Mycobacterium bovis is a transmissible disease notifiable to the World Organization for Animal Health and to the European Union, with ongoing efforts of surveillance and eradication in every EU member state. In Germany, a country which has been declared officially free from bovine t...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00302-18
更新日期:2018-08-27 00:00:00
abstract::Ceftolozane-tazobactam (C/T) is a novel beta-lactam-beta-lactamase inhibitor combination antibiotic approved by the U.S. Food and Drug Administration in 2014 for the treatment of complicated intra-abdominal infections (in combination with metronidazole) and complicated urinary tract infections. In this study, we evalu...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.00717-18
更新日期:2018-08-27 00:00:00
abstract::The iC-GPC Assay (iCubate, Huntsville, AL) is a qualitative multiplex test for the detection of five of the most common Gram-positive bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Enterococcus faecalis, and Enterococcus faecium) responsible for bacterial bloodstream infections,...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.00485-18
更新日期:2018-08-27 00:00:00
abstract::Bacterial vaginosis (BV) is the most common cause of vaginal discharge in reproductive-age women. BV has been associated with poor reproductive outcomes such as preterm delivery, the acquisition of sexually transmitted infections, including HIV, and pelvic inflammatory disease. BV represents the acquisition of a diver...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,评审
doi:10.1128/JCM.00342-18
更新日期:2018-08-27 00:00:00
abstract::The analytical performance of the AIX1000 system, a fully automated and recently FDA-cleared rapid plasma reagin (RPR) system, was evaluated by comparison to a manual RPR test in a traditional syphilis testing algorithm. A total of 1,028 consecutive serum samples submitted for syphilis testing to the University of Nor...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00214-18
更新日期:2018-07-26 00:00:00
abstract::Yersinia pestis is the causative agent of plague and is considered a category A priority pathogen due to its potential for high transmissibility and the significant morbidity and mortality it causes in humans. Y. pestis is endemic to the western United States and much of the world, necessitating programs to monitor fo...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00273-18
更新日期:2018-06-25 00:00:00
abstract::Burkholderia pseudomallei causes significant global morbidity and mortality, with the highest disease burden in parts of Asia where culture-based diagnosis is often not available. We prospectively evaluated the Active Melioidosis Detect (AMD; InBios International, USA) lateral flow immunoassay (LFI) for rapid detectio...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02002-17
更新日期:2018-06-25 00:00:00
abstract::The QuantiFERON-TB Gold Plus (QFT-Plus; Qiagen, Germantown, MD) interferon gamma release assay (IGRA) received FDA clearance in 2017 and will replace the prior version of the assay, the QFT-Gold In-Tube (QFT-GIT). Here, we compared performances of the QFT-Plus assay and the QFT-GIT version in a diverse patient populat...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00614-18
更新日期:2018-06-25 00:00:00
abstract::Quantitative real-time PCR (qPCR) is increasingly being used for the detection of bovine leukemia virus (BLV) proviral DNA. Nevertheless, quality control for the validation and standardization of such tests is currently lacking. Therefore, the present study was initiated by three Office International des Epizooties (O...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00304-18
更新日期:2018-06-25 00:00:00
abstract::A retrospective case record study was conducted that established a scoring tool based on clinical and iQ200 parameters, able to predict or rule out the clinical diagnosis of UTI in the majority of adult patients in an academic hospital. Automated standardized quantitative urine analysis, such as iQ200 analysis, is on ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02030-17
更新日期:2018-05-25 00:00:00
abstract::RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it is unclear how each vaccine strain replicates in intestinal tissue and is excreted in stool. To examine this issue, we established RV5 genotype-specific re...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00035-18
更新日期:2018-05-25 00:00:00
abstract::A DNA microarray platform, based on the nucleotide sequences of the internal transcribed spacer regions (ITS1 and ITS2) of the rRNA gene, was developed to identify 32 fungal pathogens at the species level. The probe sequences were spotted onto polycarbonate slides with a mini-microarray printer, and after the hybridiz...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01908-17
更新日期:2018-04-25 00:00:00
abstract::Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence h...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01951-17
更新日期:2018-04-25 00:00:00
abstract::INTRODUCTIONSyndromic panels were first FDA cleared for detection of respiratory pathogens in 2008. Since then, other panels have been approved by the FDA, and most recently, the FilmArray meningitis/encephalitis panel (BioFire, Salt Lake City, UT) has become available. This assay detects 14 targets within 1 h and inc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00018-18
更新日期:2018-03-26 00:00:00
abstract::Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has proved to be a useful diagnostic method for identifying conventional bacteria. In the case of mycobacteria, a good protein extraction protocol is essential in order to obtain reliable identification results. To date, no suc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01548-17
更新日期:2018-03-26 00:00:00
abstract::The use of high-risk human papillomavirus (HPV) testing for surveillance and clinical applications is increasing globally, and it is important that tests are evaluated to ensure they are fit for this purpose. In this study, the performance of a new HPV genotyping test, the Papilloplex high-risk HPV (HR-HPV) test, was ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01687-17
更新日期:2018-02-22 00:00:00
abstract::Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain-Barré syndrome in adults. Diagnosis and surv...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01785-17
更新日期:2018-02-22 00:00:00
abstract::The performance of a disk diffusion test using broth from positive blood cultures as inoculum (direct disk diffusion [dDD]) was evaluated for a collection of 20 challenge isolates of Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa Isolates seeded into human blood were inoculated into Bactec Plu...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01678-17
更新日期:2018-02-22 00:00:00
abstract::Use of whole-genome sequencing (WGS) for routine mycobacterial species identification and drug susceptibility testing (DST) is becoming a reality. We compared the performances of WGS and standard laboratory workflows prospectively, by parallel processing at a major mycobacterial reference service over the course of 1 ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01480-17
更新日期:2018-01-24 00:00:00
abstract::Little is known about the sensitivity of the BinaxNOW pneumococcal urinary antigen (PUA) test for adult pneumococcal pneumonia caused by different serotypes. In this study, we aimed to analyze the trends in the sensitivity of the PUA test over a 15-year period (2001 to 2015) and to analyze its sensitivity for pneumoco...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00787-17
更新日期:2018-01-24 00:00:00
abstract::Real-time quantitative PCR (qPCR) assay of sputum or nasopharyngeal specimens has shown promising results in the detection of pneumococcal community-acquired pneumonia (PncCAP). We applied qPCR for the autolysin gene (lytA) and compared sputum and nasopharyngeal swab (NPS) pneumococcal loads in elderly patients with c...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01231-17
更新日期:2017-12-26 00:00:00
abstract::Automated treponemal immunoassays are used for syphilis screening with the reverse-sequence algorithm; discordant results (e.g., enzyme immunoassay [EIA] reactive and reactive plasma reagin [RPR] nonreactive) are resolved with a second treponemal test. We conducted a study to determine automated immunoassay signal str...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01165-17
更新日期:2017-12-26 00:00:00
abstract::A novel lateral flow immunochromatographic device (LFD) was evaluated in several veterinary diagnostic laboratories. It was confirmed to be specific for Mycobacterium bovis and M.caprae cells. The performance of the novel LFD was assessed relative to the confirmatory tests routinely applied after culture (spoligotypin...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00728-17
更新日期:2017-12-01 00:00:00
abstract::Accurate and rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is needed to screen MRSA carriers and improve treatment. The current widely used duplex PCR methods are not able to differentiate MRSA from coexisting methicillin-susceptible S. aureus (MSSA) or other methicillin-resistant staphylo...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00716-17
更新日期:2017-10-01 00:00:00
abstract::High-dose cefepime-tazobactam (1:1; WCK 4282), a novel antibacterial combination consisting of the β-lactamase inhibitor tazobactam and a fourth-generation cephalosporin, is under clinical development for the treatment of serious Gram-negative infections. A quality control (QC) study was performed to establish disk di...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00788-17
更新日期:2017-10-01 00:00:00
abstract::Malaria is one of the leading causes of infectious disease in travelers returning from the tropics. The diagnosis of malaria is typically performed by examining Giemsa-stained thick and thin peripheral blood smears, which is time consuming, labor intensive, and requires high levels of proficiency. Alternatively, loop-...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00806-17
更新日期:2017-10-01 00:00:00
abstract::The emerging multidrug-resistant pathogenic yeast Candida auris represents a serious threat to global health. Unlike most other Candida species, this organism appears to be commonly transmitted within health care facilities and causes health care-associated outbreaks. To better understand the epidemiology of this emer...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00921-17
更新日期:2017-10-01 00:00:00
abstract::The timely and exact diagnosis of prosthetic joint infection (PJI) is crucial for surgical decision-making. Intraoperatively, delivery of the result within an hour is required. Alpha-defensin lateral immunoassay of joint fluid (JF) is precise for the intraoperative exclusion of PJI; however, for patients with a limite...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00558-17
更新日期:2017-09-01 00:00:00
abstract::The group B streptococcus (GBS) capsular polysaccharide (CPS) is an important virulence factor which is also used for GBS typing. There are 10 CPS types (Ia, Ib, and II to IX). GBS that do not phenotypically type are considered nontypeable. All genes required for CPS synthesis are found on the GBS cps operon, which co...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00300-17
更新日期:2017-09-01 00:00:00
abstract::HIV-1 non-B subtypes/circulating recombinant forms (CRFs) are increasing worldwide. Since subtype identification can be clinically relevant, we assessed the added value in HIV-1 subtyping using updated molecular phylogeny (Mphy) and the performance of routinely used automated tools. Updated Mphy (2015 updated referenc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00656-17
更新日期:2017-09-01 00:00:00
abstract::The prevalence of tuberculosis continues to be high, and nontuberculous mycobacterial (NTM) infection has also emerged worldwide. Moreover, differential and accurate identification of mycobacteria to the species or subspecies level is an unmet clinical need. Here, we developed a one-step multiplex PCR assay using whol...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00549-17
更新日期:2017-09-01 00:00:00
abstract::Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trich...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00461-17
更新日期:2017-08-01 00:00:00
abstract::Public health microbiology laboratories (PHLs) are on the cusp of unprecedented improvements in pathogen identification, antibiotic resistance detection, and outbreak investigation by using whole-genome sequencing (WGS). However, considerable challenges remain due to the lack of common standards. Here, we describe the...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00361-17
更新日期:2017-08-01 00:00:00
abstract::Azole-resistant Aspergillus fumigatus is an increasing worldwide problem with major clinical implications. Surveillance is warranted to guide clinicians to provide optimal treatment to patients. To investigate azole resistance in clinical Aspergillus isolates in our institution, a Belgian university hospital, we condu...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00520-17
更新日期:2017-08-01 00:00:00
abstract::Screening of blood for human T-cell lymphotropic virus type 1 and type 2 (HTLV-1 and -2, respectively) is important to diagnose and prevent infection and ensure the safety of blood supplies. The Elecsys HTLV-I/II assay is a newly developed, electrochemiluminescence screening assay for the detection of HTLV-1/2 infecti...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.00169-17
更新日期:2017-07-01 00:00:00
abstract::The global spread of carbapenemase-producing Enterobacteriaceae (CPE) that are often resistant to most, if not all, classes of antibiotics is a major public health concern. The NDM-1 carbapenemase is among the most worrisome carbapenemases given its rapid worldwide spread. We have developed and evaluated a lateral flo...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00248-17
更新日期:2017-07-01 00:00:00
abstract::Achromobacter species are increasingly being detected in cystic fibrosis (CF) patients, with an unclear epidemiology and impact. We studied a cohort of patients attending a Canadian adult CF clinic who had positive sputum cultures for Achromobacter species in the period from 1984 to 2013. Infection was categorized as ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02556-16
更新日期:2017-07-01 00:00:00
abstract::Drug-resistant tuberculosis (TB) is a major threat to TB control worldwide. Globally, only 40% of the 340,000 notified TB patients estimated to have multidrug-resistant-TB (MDR-TB) were detected in 2015. This study was carried out to evaluate the utility of high-resolution melt curve analysis (HRM) for the rapid and d...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02104-16
更新日期:2017-06-01 00:00:00