Evaluating and Improving Vitek MS for Identification of Clinically Relevant Species of Trichosporon and the Closely Related Genera Cutaneotrichosporon and Apiotrichum.

Abstract:

:Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trichosporon taxonomy has been reassessed, and three genera have been proposed for the pathogenic species: Trichosporon, Cutaneotrichosporon, and Apiotrichum Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has replaced old phenotypic methods for microorganism identification in clinical laboratories, but spectral profile databases have to be evaluated and improved for optimal species identification performance. Vitek MS (bioMérieux) is one of the commercially available MALDI-TOF MS platforms for pathogen identification, and its spectral profile databases remain poorly evaluated for Trichosporon, Cutaneotrichosporon, and Apiotrichum species identification. We herein evaluated and improved Vitek MS for the identification of the main clinical relevant species of Trichosporon, Cutaneotrichosporon, and Apiotrichum using a large set of strains and isolates belonging to different yeast collections in Brazil and France.

journal_name

J Clin Microbiol

authors

de Almeida JN Jr,Favero Gimenes VM,Francisco EC,Machado Siqueira LP,Gonçalves de Almeida RK,Guitard J,Hennequin C,Colombo AL,Benard G,Rossi F

doi

10.1128/JCM.00461-17

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

2439-2444

issue

8

eissn

0095-1137

issn

1098-660X

pii

JCM.00461-17

journal_volume

55

pub_type

杂志文章
  • DNA macroarray for identification and typing of Staphylococcus aureus isolates.

    abstract::A DNA macroarray containing 465 intragenic amplicons was designed to identify Staphylococcus aureus at the species level and to type S. aureus isolates. The genes selected included those encoding (i) S. aureus-specific proteins, (ii) staphylococcal and enterococcal proteins mediating antibiotic resistance and factors ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.5.2054-2064.2004

    authors: Trad S,Allignet J,Frangeul L,Davi M,Vergassola M,Couve E,Morvan A,Kechrid A,Buchrieser C,Glaser P,El-Solh N

    更新日期:2004-05-01 00:00:00

  • Identification of Staphylococcus species with the API STAPH-IDENT system.

    abstract::The API STAPH-IDENT system was compared with conventional methods for the identification of 14 Staphylococcus species. Conventional methods included the Kloos and Schleifer simplified scheme and DNA-DNA hybridization. The API STAPH-IDENT strip utilizes a battery of 10 miniaturized biochemical tests, including alkaline...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.16.3.509-516.1982

    authors: Kloos WE,Wolfshohl JF

    更新日期:1982-09-01 00:00:00

  • Molecular characterization of methicillin-resistant Staphylococcus aureus isolates in Korea.

    abstract::We used several molecular typing methods to analyze 196 methicillin-resistant Staphylococcus aureus (MRSA) and 139 methicillin-susceptible S. aureus (MSSA) isolates collected between 1996 and 2005. The sequence type 72 MRSA has increased in frequency in the community in the Republic of Korea and in hospitals in recent...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00098-11

    authors: Kim ES,Lee HJ,Chung GT,Lee YS,Shin DH,Jung SI,Song KH,Park WB,Kim NJ,Park KU,Kim EC,Oh MD,Kim HB

    更新日期:2011-05-01 00:00:00

  • Genetic similarity and maintenance of Candida albicans strains from a group of AIDS patients, demonstrated by DNA fingerprinting.

    abstract::By using the computer-assisted Dendron system to analyze the patterns of Southern blots probed with the repetitive sequence Ca3, we have compared oral isolates of Candida spp. from a group of 11 nonhospitalized patients with AIDS suffering from recurrent episodes of oral thrush in Leicester, England, with oral isolate...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.4.935-941.1992

    authors: Schmid J,Odds FC,Wiselka MJ,Nicholson KG,Soll DR

    更新日期:1992-04-01 00:00:00

  • Comparison of sample preparation methods for detection of Legionella pneumophila in culture-positive bronchoalveolar lavage fluids by PCR.

    abstract::A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.0...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.7.2120-2122.1998

    authors: Jaulhac B,Reyrolle M,Sodahlon YK,Jarraud S,Kubina M,Monteil H,Piémont Y,Etienne J

    更新日期:1998-07-01 00:00:00

  • Comparison of phenotypic and genotypic methods for detection of diphtheria toxin among isolates of pathogenic corynebacteria.

    abstract::We have compared molecular, immunochemical, and cytotoxic assays for the detection of diphtheria toxin from 55 isolates of Corynebacterium diphtheriae and Corynebacterium ulcerans originally isolated in five different countries. The suitabilities and accuracies of these assays for the laboratory diagnosis of diphtheri...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.11.3173-3177.1998

    authors: Efstratiou A,Engler KH,Dawes CS,Sesardic D

    更新日期:1998-11-01 00:00:00

  • Bacterial characteristics in relation to clinical source of Escherichia coli isolates from women with acute cystitis or pyelonephritis and uninfected women.

    abstract::Characteristics differentiating Escherichia coli strains that cause cystitis or pyelonephritis from fecal E. coli remain incompletely defined, particularly among adult women in the United States. Accordingly, phylogenetic group, O antigens, and virulence factors (VFs) were analyzed among 329 E. coli isolates from the ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.12.6064-6072.2005

    authors: Johnson JR,Owens K,Gajewski A,Kuskowski MA

    更新日期:2005-12-01 00:00:00

  • Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.

    abstract::Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. Ho...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.7.2484-2487.2000

    authors: Bidet P,Lalande V,Salauze B,Burghoffer B,Avesani V,Delmée M,Rossier A,Barbut F,Petit JC

    更新日期:2000-07-01 00:00:00

  • Cryptococcal antigen test revisited: significance for cryptococcal meningitis therapy monitoring in a tertiary chinese hospital.

    abstract::For a total of 29 non-human immunodeficiency virus 1 cryptococcal meningitis cases, titer changes in the latex agglutination test before and after therapy were reviewed along with clinical manifestations, laboratory findings, and therapy regimens. The cryptococcal antigen titer decreased for every case after therapy a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.6.2989-2990.2005

    authors: Lu H,Zhou Y,Yin Y,Pan X,Weng X

    更新日期:2005-06-01 00:00:00

  • Prolonged Bartonella bacteremia in cats associated with cat-scratch disease patients.

    abstract::Recent evidence supports a causal relationship between Bartonella (Rochalimaea) henselae, cat-scratch disease (CSD), and bacillary angiomatosis. Cats appear to be the primary reservoir. Blood from 19 cats owned by 14 patients diagnosed with CSD was cultured. Blood samples from cats owned by veterinary students (n = 25...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.12.3245-3251.1995

    authors: Kordick DL,Wilson KH,Sexton DJ,Hadfield TL,Berkhoff HA,Breitschwerdt EB

    更新日期:1995-12-01 00:00:00

  • Microevolution of Helicobacter pylori type IV secretion systems in an ulcer disease patient over a ten-year period.

    abstract::Helicobacter pylori cagA and vacA genotypes have been used for almost a decade as stable entities to link the severity of gastritis and ulcer disease. We describe here microevolution of the two genomic islands, cag pathogenicity island (cagPAI; 40 kb) and tfs3 (16 kb) from isolates obtained at inclusion (one subclone)...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01631-07

    authors: Alvi A,Devi SM,Ahmed I,Hussain MA,Rizwan M,Lamouliatte H,Mégraud F,Ahmed N

    更新日期:2007-12-01 00:00:00

  • Rapid and sensitive assay for detection of enterotoxigenic Bacteroides fragilis.

    abstract::Bacteroides fragilis is an obligatory anaerobic, gram-negative bacterium found among the normal intestinal flora of humans. Enterotoxigenic strains of B. fragilis (ETBF) have been associated with diarrheal diseases in humans and animals. The enterotoxin of ETBF induces fluid changes in ligated intestinal segments and ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.12.3545-3548.1998

    authors: Zhang G,Weintraub A

    更新日期:1998-12-01 00:00:00

  • Interlaboratory Comparison of Six Real-Time PCR Assays for Detection of Bovine Leukemia Virus Proviral DNA.

    abstract::Quantitative real-time PCR (qPCR) is increasingly being used for the detection of bovine leukemia virus (BLV) proviral DNA. Nevertheless, quality control for the validation and standardization of such tests is currently lacking. Therefore, the present study was initiated by three Office International des Epizooties (O...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00304-18

    authors: Jaworski JP,Pluta A,Rola-Łuszczak M,McGowan SL,Finnegan C,Heenemann K,Carignano HA,Alvarez I,Murakami K,Willems L,Vahlenkamp TW,Trono KG,Choudhury B,Kuźmak J

    更新日期:2018-06-25 00:00:00

  • Four methods for identification of gram-negative nonfermenting rods: organisms more commonly encountered in clinical specimens.

    abstract::Four commercial kits, Oxi/Ferm (OF), API 20E (AP), Minitek (MT; BBL Microbiology Systems), and Flow N/F (NF), were evaluated, without additional tests, for identification of 258 gram-negative nonfermentative rods. OF and MT were read after 48 h of incubation, and AP and NF were read after both 24 and 48 h of incubatio...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.12.2.271-278.1980

    authors: Appelbaum PC,Stavitz J,Bentz MS,von Kuster LC

    更新日期:1980-08-01 00:00:00

  • Identification of nocardiae and streptomycetes of medical importance.

    abstract::During a taxonomic investigation of Nocardia spp. and Streptomyces spp., we received 658 isolates from laboratories of both human and veterinary medicine. Our procedure leading to the identification of 92% of these isolates, the species that they represented, and a characteristic pattern of properties of the strains o...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.6.728-736.1980

    authors: Mishra SK,Gordon RE,Barnett DA

    更新日期:1980-06-01 00:00:00

  • Cerebral aspergillosis caused by Aspergillus granulosus.

    abstract::Disseminated disease by Aspergillus granulosus has been reported only once previously in a cardiac transplant recipient. We report a fatal central nervous system infection in a lung transplant recipient. Key features of this species in the section Usti include growth at 37 degrees C and large, randomly spaced aggregat...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00588-09

    authors: Sutton DA,Wickes BL,Romanelli AM,Rinaldi MG,Thompson EH,Fothergill AW,Dishop MK,Elidemir O,Mallory GB,Moonnamakal SP,Adesina AM,Schecter MG

    更新日期:2009-10-01 00:00:00

  • Comparison by studies in squirrel monkeys, chimpanzees, and adult humans of avian-human influenza A virus reassortants derived from different avian influenza virus donors.

    abstract::We evaluated the abilities of three different avian influenza A viruses to attenuate the wild-type human influenza A/Korea/1/82 (H3N2) virus in squirrel monkeys, chimpanzees, and adult seronegative human volunteers. Two of these, avian influenza A/Mallard/NY/78 and A/Mallard/Alberta/76 viruses, appeared to be satisfac...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.24.3.467-469.1986

    authors: Snyder MH,Clements ML,Herrington D,London WT,Tierney EL,Murphy BR

    更新日期:1986-09-01 00:00:00

  • Direct detection and genotyping of Klebsiella pneumoniae carbapenemases from urine by use of a new DNA microarray test.

    abstract::Klebsiella pneumoniae carbapenemases (KPCs) are considered a serious threat to antibiotic therapy, as they confer resistance to carbapenems, which are used to treat extended-spectrum beta-lactamase (ESBL)-producing bacteria. Here, we describe the development and evaluation of a DNA microarray for the detection and gen...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00990-12

    authors: Peter H,Berggrav K,Thomas P,Pfeifer Y,Witte W,Templeton K,Bachmann TT

    更新日期:2012-12-01 00:00:00

  • Measurement of neutralizing antibody to equid herpesvirus 1 by single radial hemolysis.

    abstract::Antibody to equid herpesvirus 1, which mediates single radial hemolysis, is that responsible for neutralization. Hemagglutination inhibition antibody is not necessarily involved in neutralization or hemolysis. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Klingeborn B,Dinter Z

    更新日期:1978-05-01 00:00:00

  • Meningococcal Deduced Vaccine Antigen Reactivity (MenDeVAR) Index: a Rapid and Accessible Tool That Exploits Genomic Data in Public Health and Clinical Microbiology Applications.

    abstract::As microbial genomics makes increasingly important contributions to clinical and public health microbiology, the interpretation of whole-genome sequence data by nonspecialists becomes essential. In the absence of capsule-based vaccines, two protein-based vaccines have been used for the prevention of invasive serogroup...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02161-20

    authors: Rodrigues CMC,Jolley KA,Smith A,Cameron JC,Feavers IM,Maiden MCJ

    更新日期:2020-12-17 00:00:00

  • Full-genome sequence analysis of a multirecombinant echovirus 3 strain isolated from sewage in Greece.

    abstract::An echovirus 3 (Echo3) strain (strain LR31G7) was isolated from a sewage treatment plant in Greece in 2005. Full-genome molecular, phylogenetic, and SimPlot analyses were conducted in order to reveal the evolutionary pathways of the isolate. Nucleotide and phylogenetic analyses of part of the VP1 genomic region reveal...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00475-09

    authors: Kyriakopoulou Z,Dedepsidis E,Pliaka V,Tsakogiannis D,Pratti A,Levidiotou-Stefanou S,Markoulatos P

    更新日期:2010-05-01 00:00:00

  • Novel intestinal Helicobacter species isolated from cotton-top tamarins (Saguinus oedipus) with chronic colitis.

    abstract::A disease similar to ulcerative colitis in humans has been identified in cotton-top tamarins (CTTs) in captivity. The clinical signs include weight loss, diarrhea, and rectal bleeding with the pathological features and biochemical abnormalities of ulcerative colitis. Approximately 25 to 40% of these animals develop co...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.1.146-151.1999

    authors: Saunders KE,Shen Z,Dewhirst FE,Paster BJ,Dangler CA,Fox JG

    更新日期:1999-01-01 00:00:00

  • DNA fingerprinting of Mycobacterium tuberculosis complex culture isolates collected in Brazil and spotted onto filter paper.

    abstract::The usefulness of filter paper for preservation of bacterial cells was shown by mixed-linker DNA fingerprint analysis of Mycobacterium tuberculosis isolates from 77 Brazilian patients. DNA fingerprints of samples spotted onto filter paper and conventional culture material were identical. Thus, filter paper specimens a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.2.573-576.1998

    authors: Burger M,Raskin S,Brockelt SR,Amthor B,Geiss HK,Haas WH

    更新日期:1998-02-01 00:00:00

  • Molecular epidemiology of the sil streptococcal invasive locus in group A streptococci causing invasive infections in French children.

    abstract::We found 31 different emm-toxin genotypes among 74 group A streptococcal isolates causing invasive infections in French children. The predominant emm types were emm1 (25%), emm3 (8%), emm4 (8%), emm6 (7%), and emm89 (9%). Sixteen percent of isolates harbored the streptococcal invasive locus, half of them belonging to ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00104-07

    authors: Bidet P,Courroux C,Salgueiro C,Carol A,Mariani-Kurkdjian P,Bonacorsi S,Bingen E

    更新日期:2007-06-01 00:00:00

  • Antibody to a 39-kilodalton Borrelia burgdorferi antigen (P39) as a marker for infection in experimentally and naturally inoculated animals.

    abstract::Borrelia burgdorferi expresses a conserved, species-specific 39-kDa protein (P39) that can stimulate antibodies during human infection. To confirm that anti-P39 antibodies are produced consistently in animals exposed to infectious spirochetes, white-footed mice, Peromyscus leucopus, and laboratory white mice, Mus musc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.2.236-243.1991

    authors: Simpson WJ,Burgdorfer W,Schrumpf ME,Karstens RH,Schwan TG

    更新日期:1991-02-01 00:00:00

  • Characteristics of Bacteroides asaccharolyticus from dental plaques of beagle dogs.

    abstract::Gram-negative, non-saccharolytic, brown- or black-pigment-forming, nonmotile anaerboic coccobacilli, capable of decomposing hydrogen peroxide and identified as Bacteroides asaccharolyticus (B. melaninogenicus subsp. asaccharolyticus), were isolated from the supra- and subgingival plaques of beagle dogs with gingivitis...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.5.522-526.1980

    authors: Syed SA

    更新日期:1980-05-01 00:00:00

  • New blood culture medium.

    abstract::A growth medium with a specific oxidation-reduction potential containing peptone, dextrose, sodium succinate, sodium lactate, gelatin, sodium bicarbonate and blue tetrazolium, an indicator dye, in a tris(hydroxymethyl)aminomethane buffer was used to detect the presence of microorganisms in blood. The procedure involve...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Shih CN,Balish E

    更新日期:1977-09-01 00:00:00

  • Purification of sputum samples through sucrose improves detection of Mycobacterium tuberculosis by polymerase chain reaction.

    abstract::A method is described for the routine preparation of sputum samples for detection of Mycobacterium tuberculosis by using polymerase chain reaction amplification. Liquefaction of sputum samples with NaOH and subsequent removal of inhibitors of the polymerase reaction with a 50% sucrose centrifugation step (5 min) in a ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.6.1514-1517.1992

    authors: Victor T,du Toit R,van Helden PD

    更新日期:1992-06-01 00:00:00

  • Chimeric antigens of Toxoplasma gondii: toward standardization of toxoplasmosis serodiagnosis using recombinant products.

    abstract::We have evaluated the diagnostic utility of six antigenic regions of the Toxoplasma gondii MIC2, MIC3, M2AP, GRA3, GRA7, and SAG1 gene products, assembled in recombinant chimeric antigens by genetic engineering, in order to replace the soluble, whole-cell tachyzoite extract in serological assays. Serum samples from 10...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00237-06

    authors: Beghetto E,Spadoni A,Bruno L,Buffolano W,Gargano N

    更新日期:2006-06-01 00:00:00

  • First Spanish case of nocardiosis caused by Nocardia takedensis.

    abstract::Nocardia takedensis is a recently described species isolated from soil. The first clinical isolate in Japan has recently been reported. This report describes the first clinical isolate of N. takedensis in Spain from a respiratory specimen. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00090-09

    authors: Betrán A,Rezusta A,Lezcano MA,Villuendas MC,Revillo MJ,Boiron P,Rodríguez-Nava V

    更新日期:2009-06-01 00:00:00