Abstract:
:By using the computer-assisted Dendron system to analyze the patterns of Southern blots probed with the repetitive sequence Ca3, we have compared oral isolates of Candida spp. from a group of 11 nonhospitalized patients with AIDS suffering from recurrent episodes of oral thrush in Leicester, England, with oral isolates from a group of control individuals. Genetic diversity among the AIDS strains was significantly reduced compared with that of control strains. In addition, the same strains persisted through recurrent infections in patients with AIDS. Although AIDS strains were genetically less diverse than either control strains or oral commensal strains analyzed in previous studies, the majority did not form a genetically distinct group. The results of this study suggest that in the majority of patients with AIDS in this group from Leicester, original commensal strains were replaced, replacement occurred early in the manifestation of AIDS, and replacement occurred only once.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Schmid J,Odds FC,Wiselka MJ,Nicholson KG,Soll DRdoi
10.1128/JCM.30.4.935-941.1992subject
Has Abstractpub_date
1992-04-01 00:00:00pages
935-41issue
4eissn
0095-1137issn
1098-660Xjournal_volume
30pub_type
杂志文章abstract::By the use of two reference procedures, an indirect hemagglutination assay and a complement fixation test, the presence or absence of cytomegalovirus (CMV) antibody was determined for 221 human sera. Ninety-nine sera (44.8%) were found to contain CMV antibody. The remaining 122 sera (55.2%) lacked detectable CMV antib...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.20.1.9-11.1984
更新日期:1984-07-01 00:00:00
abstract::Enterobacteriaceae represent a diverse and medically important family of bacteria that are difficult to identify to the species level using the standard molecular method of 16S rRNA gene sequencing. Prior work has demonstrated the value of dnaJ gene sequence analysis in resolving different members of the family. Howev...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00986-19
更新日期:2019-10-23 00:00:00
abstract::The contamination of semiclosed disposable circuits of Healthdyne and Bourns ventilators was studied in a newborn intensive care unit over a 2-year period. A total of 379 fluid samples was obtained from inspiratory and expiratory tubing condensates and traps and from thermal humidifier columns fed with prefilled conta...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.23.2.322-328.1986
更新日期:1986-02-01 00:00:00
abstract::Several studies have identified thymidine excess in susceptibility test media as the cause of spurious resistance of various bacteria to sulfamethoxazole-trimethoprim. We document the phenomenon in Salmonella typhi and Salmonella paratyphi-A and demonstrate its occurrence in 3 of 17 (18%) lots of Mueller-Hinton agars ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.23.1.205-206.1986
更新日期:1986-01-01 00:00:00
abstract::The polymerase chain reaction was used to detect human herpesvirus 6 (HHV-6) DNA in peripheral blood mononuclear cells from patients with exanthem subitum. Amplified products were detected by agarose gel electrophoresis and dot blot hybridization with a cloned DNA probe. No cross-hybridization with DNAs of five other ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.5.970-974.1990
更新日期:1990-05-01 00:00:00
abstract::The sensitivity and specificity of the human immunodeficiency virus (HIV) type 1-specific immunoglobulin G capture enzyme-linked immunosorbent assay (BED-CEIA) were compared with those of the avidity index method to identify recent HIV infection using a panel of 148 samples (81 patients) representing durations of infe...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01055-07
更新日期:2008-01-01 00:00:00
abstract::The DNA fingerprint profiles and somatic serotypes of 71 Pasteurella multocida capsule serogroup B isolates, 13 capsule serogroup E isolates, and 16 somatic reference serotype strains were compared. Each of the 16 reference somatic serotypes had a unique DNA fingerprint profile with the HhaI restriction endonuclease. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.6.1518-1524.1992
更新日期:1992-06-01 00:00:00
abstract::The 37-kDa protein (P37) of Borrelia burgdorferi is an antigen that elicits an early immunoglobulin M (IgM) antibody response in Lyme disease patients. The P37 gene was cloned from a B. burgdorferi genomic library by screening with antibody from a Lyme disease patient who had developed a prominent humoral response to ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.3.548-552.1999
更新日期:1999-03-01 00:00:00
abstract::Meningococcal disease caused by N. meningitidis serogroup B (MenB) has been endemic in Brazil since 1997. In this study, we determined the prevalence of serosubtypes of MenB isolated in 10 Brazilian states and the Federal District during 1997 and 1998 and investigated the extent of PorA VR sequence variation among the...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.8.2897-2903.2001
更新日期:2001-08-01 00:00:00
abstract::The Autobac IDX system (General Diagnostics, Warner-Lambert Co., Morris Plains, N.J.) for rapid, semiautomated identification of gram-negative bacilli was compared with the identification methods in routine use in four laboratories. The study included 1,515 organisms representing 30 species of enteric and nonenteric b...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.19.4.529-533.1984
更新日期:1984-04-01 00:00:00
abstract::We describe here the use of an immunomagnetic separation enrichment process coupled with a modified real-time cellular analysis (RTCA) system (RTCA version 2) for the detection of C. difficile toxin (CDT) in stool. The limit of CDT detection by RTCA version 2 was 0.12 ng/ml. Among the consecutively collected 401 diarr...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02601-13
更新日期:2014-04-01 00:00:00
abstract::In acute and chronic hepatitis B, antibodies of the immunoglobulin M (IgM) class against the hepatitis B core antigen (anti-HBc IgM) have been demonstrated. For the determination of anti-HBc IgM, a sensitive enzyme immunoassay with anti-mu-coated flat-bottomed microtiter plates is described and evaluated. The specific...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.13.4.618-626.1981
更新日期:1981-04-01 00:00:00
abstract::Serial cerebrospinal fluid samples were obtained from 14 multiple sclerosis patients over a period of several months and were tested under code for Inoue-Melnick virus. In five of the positive patients, virus was present in 28 of 32 specimens collected over a period of 2 to 5 months. Four patients, from whom a total o...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.22.4.651-653.1985
更新日期:1985-10-01 00:00:00
abstract::Twelve bacterial species including Streptococcus uberis, S. parauberis, S. agalactiae, S. dysgalactiae, S. bovis, S. mitis, S. salivarius, S. saccharolyticus, Enterococcus faecium, E. faecalis, E. avium, and Aerococcus viridans were examined for their 16S ribosomal DNA fingerprint patterns. Oligonucleotide primers com...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.9.2235-2240.1992
更新日期:1992-09-01 00:00:00
abstract::We evaluated the clinical performance of Check-Direct CPE for carbapenemase detection directly from 301 perirectal swabs (258 patients) in a nonoutbreak setting. Culture of a PCR-confirmed, carbapenemase-containing organism, or history of colonization with such organism within the previous 2 weeks, was used as the ref...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01921-15
更新日期:2015-12-01 00:00:00
abstract::We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia s...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02340-14
更新日期:2014-11-01 00:00:00
abstract::The CO2, O2, and pH levels of commercially available blood culture bottles with tryptic soy broth medium from five different manufacturers were compared. Ranges of 1.3 to 6.9% for CO2, 1.1 to 6.0% for O2, and pH 6.94 to 7.26 were found. Different venting procedures revealed that blood culture bottles from which the ru...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.20.5.881-883.1984
更新日期:1984-11-01 00:00:00
abstract::Of 100 strains of enterococci, 83% reduced litmus milk within 4 h. None of the 100 non-enterococcal streptococci tested were positive. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.14.2.227-228.1981
更新日期:1981-08-01 00:00:00
abstract::The detection and quantitation of human parainfluenza virus type 1 (HPIV-1) RNA in nasal wash specimens from 49 children with lower respiratory infections were performed by a reverse transcription-PCR-enzyme hybridization assay (RT-PCR-EHA). The HPIV-1 RT-PCR-EHA was then used to test 40 samples from asymptomatic chil...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.8.1914-1917.1996
更新日期:1996-08-01 00:00:00
abstract::In a prospective, multicenter study of 342 blood samples from 187 patients with systemic inflammatory response syndrome, sepsis, or neutropenic fever, a new commercial PCR test (SepsiTest; Molzym) was evaluated for rapid diagnosis of bacteremia. The test comprises a universal PCR from the 16S rRNA gene, with subsequen...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.00567-09
更新日期:2009-09-01 00:00:00
abstract::Clostridium difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis, which have significant morbidity and mortality. Accurate and timely diagnosis is critical. Repeat enzyme immunoassay testing for C. difficile toxin has been recommended because of <100% sensitivity. All C. diffic...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00931-08
更新日期:2008-11-01 00:00:00
abstract::The Mycobacterium tuberculosis complex includes the four species M. tuberculosis, M. bovis, M. africanum, and M. microti. We sequenced 13 M. tuberculosis complex strains in the 16S-to-23S rDNA internal transcribed spacer (ITS). The ITS has a high rate of nucleotide substitution. Previous reports found three nucleotide...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.7.1639-1643.1994
更新日期:1994-07-01 00:00:00
abstract::Pneumocystis jirovecii pneumonia (PCP) is a life-threatening infection in immunocompromised patients. Quantitative real-time PCR (qPCR) is more sensitive than microscopic examination for the detection of P. jirovecii but also detects colonized patients. Hence, its positive predictive value (PPV) needs evaluation. In t...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01480-14
更新日期:2014-09-01 00:00:00
abstract::PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a rapid and convenient technique for the detection of mutations and allelic variants. We have adapted this technique for the identification of bacteria by PCR with fluorescein-labeled primers chosen from the conserved regions of the 16S rRNA gene flank...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.10.2601-2606.1995
更新日期:1995-10-01 00:00:00
abstract::A procedure was developed for characterization of Chlamydia trachomatis strains by using restriction endonuclease analysis of amplified genes of the major outer membrane protein (MOMP). Reference strains of the 15 serovars (A through K and L1 through L3) and clinical isolates were tested. The nucleotide sequences of t...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.6.1132-1136.1991
更新日期:1991-06-01 00:00:00
abstract::Immunoglobulin M (IgM) and IgA antibodies against the major surface protein of Toxoplasma gondii were determined in a total of 195 human sera and five human cerebrospinal fluids by using a P30 membrane extract and the immunoblot technique. By using two different T. gondii strains (RH and BK) simultaneously as antigens...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.6.1436-1441.1992
更新日期:1992-06-01 00:00:00
abstract::Antigens of Rhodococcus equi were analyzed by immunoblotting with naturally infected foal sera. Immunoblots of whole-cell antigen preparations of clinical isolates of R. equi revealed that major protein bands with molecular masses of 15 to 17 kDa were present in all clinical isolates tested and all isolates virulent f...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.3.439-443.1991
更新日期:1991-03-01 00:00:00
abstract::More than 700 bacterial species or phylotypes, of which over 50% have not been cultivated, have been detected in the oral cavity. Our purposes were (i) to utilize culture-independent molecular techniques to extend our knowledge on the breadth of bacterial diversity in the healthy human oral cavity, including not-yet-c...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.11.5721-5732.2005
更新日期:2005-11-01 00:00:00
abstract::An evaluation was undertaken to determine the optimal method for the in vitro susceptibility testing of 26 Nocardia asteroides complex isolates to the following antimicrobial agents: amikacin, ampicillin, amoxicillin-clavulanate, ceftriaxone, ciprofloxacin, erythromycin, imipenem, minocycline, and trimethoprim-sulfame...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.4.847-852.1997
更新日期:1997-04-01 00:00:00
abstract::An in situ DNA hybridization kit for cytomegalovirus (CMV) was evaluated for the detection of CMV in centrifugation culture. Of 61 clinical specimens, 17 (27.8%) were positive for CMV by monoclonal antibody staining following centrifugation. Of the 17 positive specimens, 15 were detected by DNA hybridization (24.5%). ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.1.21-23.1989
更新日期:1989-01-01 00:00:00