Identification of nocardiae and streptomycetes of medical importance.

abstract：:There is a greatly increased interest in Herpesvirus hominis infections especially those of type 2 associated with genital lesions or neonatal disease. Physicians are eager to confirm the clinical impression with a specific virologic diagnosis such as isolation of the agent and its typing, and type-specific antibody r...

journal_title：Journal of clinical microbiology

authors： Hanna L,Keshishyan H,Jawetz E,Coleman VR

更新日期：1975-03-01 00:00:00

abstract：:Yersinia enterocolitica is known to be associated with gastroenteritis in children and Reiter's syndrome in adults, but it has only rarely been the cause of primary soft tissue infections. A patient with diabetes mellitus developed a calf abscess, from which Y. enterocolitica was isolated in pure culture. Incision, dr...

journal_title：Journal of clinical microbiology

authors： Brennessel DJ,Robbins N,Hindman S

更新日期：1984-08-01 00:00:00

abstract：:Clinical and experimental observations suggest that immune globulin may prevent otitis media (OM) in children. We performed experiments in chinchillas to test the hypothesis that human bacterial polysaccharide immune globulin (BPIG) might prevent OM caused by Streptococcus pneumoniae. Animals were given BPIG or saline...

journal_title：Journal of clinical microbiology

authors： Shurin PA,Giebink GS,Wegman DL,Ambrosino D,Rholl J,Overman M,Bauer T,Siber GR

更新日期：1988-04-01 00:00:00

abstract：:Sheep and human erythrocytes, partially processed by Staphylococcus aureus or Clostridium perfringens, were susceptible to lysis in the presence of Propionibacterium acnes. P. acnes liberated a lipase that was detected on Tween 80 agar and also on phospholipase C-precipitated egg yolk agar. Such a lipase might have co...

journal_title：Journal of clinical microbiology

authors： Choudhury TK

更新日期：1978-08-01 00:00:00

abstract：:Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was collected from children with bullous impetigo in 2003 and 2004. One strain collected in 2003 was Panton-Valentine leucocidin (PVL) positive. In 2004, a multiple-drug-resistant PVL(+) CA-MRSA strain was isolated from an athlete with a cutaneou...

journal_title：Journal of clinical microbiology

authors： Takizawa Y,Taneike I,Nakagawa S,Oishi T,Nitahara Y,Iwakura N,Ozaki K,Takano M,Nakayama T,Yamamoto T

更新日期：2005-07-01 00:00:00

abstract：:Recent studies have shown the public health importance of identifying individuals with acute human immunodeficiency virus infection (AHI); however, the cost of nucleic acid amplification testing (NAAT) makes individual testing of at-risk individuals prohibitively expensive in many settings. Pooled NAAT (or group testi...

journal_title：Journal of clinical microbiology

authors： Westreich DJ,Hudgens MG,Fiscus SA,Pilcher CD

更新日期：2008-05-01 00:00:00

abstract：:We previously demonstrated that whole blood contains significantly more hepatitis C virus (HCV) RNA than plasma. To validate the whole-blood-based HCV RNA detection method, a prospective comparison of HCV RNA detection in whole blood and plasma from 50 patients with chronic liver disease was undertaken. Whole-blood an...

journal_title：Journal of clinical microbiology

authors： Stapleton JT,Klinzman D,Schmidt WN,Pfaller MA,Wu P,LaBrecque DR,Han Jq,Phillips MJ,Woolson R,Alden B

更新日期：1999-03-01 00:00:00

abstract：:The toluidine red unheated serum test (TRUST) antigen, a macroscopic flocculation test antigen developed by Pettit et al. (J. Clin. Microbiol. 18:1141-1145, 1983) by modifying the color-coded antigen of Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974), was compared with the Venereal Disease Research Laboratory...

journal_title：Journal of clinical microbiology

authors： Parham CE,Pettit DE,Larsen SA,Hambie EA,Perryman MW,McGrew BE

更新日期：1984-09-01 00:00:00

abstract：:The identification of Pasteurella and related bacteria remains a challenge. Here, a 449- to 473-bp fragment (sodA(int)) internal to the sodA gene, encoding the manganese-dependent superoxide dismutase, was amplified and sequenced with a single pair of degenerate primers from the type strains of Pasteurella (18 strains...

journal_title：Journal of clinical microbiology

authors： Gautier AL,Dubois D,Escande F,Avril JL,Trieu-Cuot P,Gaillot O

更新日期：2005-05-01 00:00:00

abstract：:Cerebrospinal fluid samples from 257 patients with suspected herpes simplex virus encephalitis were prospectively analyzed by herpes simplex virus polymerase chain reaction. The polymerase chain reaction indicated herpes simplex virus encephalitis in 9 serologically proven cases and in 14 additional patients. Increase...

journal_title：Journal of clinical microbiology

authors： Puchhammer-Stöckl E,Heinz FX,Kundi M,Popow-Kraupp T,Grimm G,Millner MM,Kunz C

更新日期：1993-01-01 00:00:00

abstract：:The analytical performance of the AIX1000 system, a fully automated and recently FDA-cleared rapid plasma reagin (RPR) system, was evaluated by comparison to a manual RPR test in a traditional syphilis testing algorithm. A total of 1,028 consecutive serum samples submitted for syphilis testing to the University of Nor...

journal_title：Journal of clinical microbiology

authors： Sanfilippo AM,Freeman K,Schmitz JL

更新日期：2018-07-26 00:00:00

abstract：:Community methicillin-resistant Staphylococcus aureus (CMRSA) strains are being isolated with increasing frequency around the world. In Western Australia CMRSA are endemic in geographically remote communities and have been found to belong to five different contour-clamped homogeneous electric field (CHEF) electrophore...

journal_title：Journal of clinical microbiology

authors： O'Brien FG,Lim TT,Chong FN,Coombs GW,Enright MC,Robinson DA,Monk A,Saïd-Salim B,Kreiswirth BN,Grubb WB

更新日期：2004-07-01 00:00:00

abstract：:Detection of the source of Clostridium difficile strains is of importance for the control of the nosocomial spread of this microorganism. For this purpose, vaginal and rectal swabs from 183 mothers, duplicate fecal samples (taken on days 1 and 4 after birth) from 183 neonates, and 94 environmental samples were culture...

journal_title：Journal of clinical microbiology

authors： Martirosian G,Kuipers S,Verbrugh H,van Belkum A,Meisel-Mikolajczyk F

更新日期：1995-08-01 00:00:00

abstract：:Specimens of colonic mucosa from two pigs with diarrhea were examined by light and electron microscopy. The epithelial surfaces of both pigs were extensively colonized by large spirochetes morphologically compatible with Treponema hyodysenteriae or Treponema innocens. The microorganisms were intimately attached end-on...

journal_title：Journal of clinical microbiology

authors： Jacques M,Girard C,Higgins R,Goyette G

更新日期：1989-05-01 00:00:00

abstract：:In order to differentiate species within the Borrelia burgdorferi sensu lato complex, LightCyler PCR and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the recA gene, were performed. It was demonstrated that nested LightCycler PCR amplification of p66 is more sensi...

journal_title：Journal of clinical microbiology

authors： Mommert S,Gutzmer R,Kapp A,Werfel T

更新日期：2001-07-01 00:00:00

abstract：:A nonpigmented rapidly growing mycobacterium was isolated from wound liquid outflow, bone tissue biopsy, and excised skin tissue from a 31-year-old woman who suffered an accidental open right tibia fracture and prolonged stay in a river. The three isolates grew in 3 days at 24 to 37 degrees C. 16S rRNA sequence analys...

journal_title：Journal of clinical microbiology

authors： Adékambi T,Stein A,Carvajal J,Raoult D,Drancourt M

更新日期：2006-04-01 00:00:00

abstract：:Optimal conditions for a fluorescence immunoassay of antibodies to, and surface antigens of, Actinomyces viscosus ATCC 19246 are described. In the standard fluorescence immunoassay, 10(8) colony-forming units of A. viscosus reacted with an antibody preparation, were washed, and then were treated with an excess of fluo...

journal_title：Journal of clinical microbiology

authors： Gillis TP,Thompson JJ

更新日期：1978-02-01 00:00:00

abstract：:In Brazil, Salmonella enterica serovar Infantis resistant to various antimicrobials, including cephalosporins, has been identified as an etiological agent of severe gastroenteritis in hospitalized children since 1994. In this study, 35 serovar Infantis strains, isolated from children admitted to four different Rio de ...

journal_title：Journal of clinical microbiology

authors： Fonseca EL,Mykytczuk OL,Asensi MD,Reis EM,Ferraz LR,Paula FL,Ng LK,Rodrigues DP

更新日期：2006-08-01 00:00:00

abstract：:Marburg virus (MARV) causes a severe hemorrhagic fever in humans with a high mortality rate. The rapid and accurate identification of the virus is required to appropriately provide infection control and outbreak management. Here, we developed and evaluated a one-step reverse transcription-loop-mediated isothermal ampl...

journal_title：Journal of clinical microbiology

authors： Kurosaki Y,Grolla A,Fukuma A,Feldmann H,Yasuda J

更新日期：2010-07-01 00:00:00

abstract：:A passive hemagglutination (PHA) assay for antibody to Pseudomonas aeruginosa exotoxin is described which utilizes chromic chloride-treated ovine erythrocytes coated with purified toxin. PHA antitoxin titers correlated well with those obtained by a cytotoxicity neutralization assay (r = 0.91, P less than 0.001), where...

journal_title：Journal of clinical microbiology

authors： Pollack M,Taylor NS

更新日期：1977-07-01 00:00:00

abstract：:The results of single radial immunodiffusion assays of influenza virus hemagglutinin were found to be greatly altered by small antigenic differences between test and reference strains. When such differences were present, the precise specificity of the antiserum used had a critical effect on the measured hemagglutinin ...

journal_title：Journal of clinical microbiology

authors： Rodda SJ,Gallichio HA,Hampson AW

更新日期：1981-11-01 00:00:00

abstract：:Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trich...

journal_title：Journal of clinical microbiology

authors： de Almeida JN Jr,Favero Gimenes VM,Francisco EC,Machado Siqueira LP,Gonçalves de Almeida RK,Guitard J,Hennequin C,Colombo AL,Benard G,Rossi F

更新日期：2017-08-01 00:00:00

abstract：:Four slightly yellow-pigmented, alpha-hemolytic, gram-negative coccobacilli, three from wound specimens and one from multiple blood cultures of a patient with endocarditis, were identified as Neisseria elongata subsp. glycolytica on the basis of their overall biochemical and genetic similarities to this subspecies. Th...

journal_title：Journal of clinical microbiology

authors： Andersen BM,Weyant RS,Steigerwalt AG,Moss CW,Hollis DG,Weaver RE,Ashford D,Brenner DJ

更新日期：1995-01-01 00:00:00

abstract：:Hepatitis E virus (HEV) is a major cause of hepatitis. We evaluated five HEV antibody diagnostic assays by using outbreak specimens. The Abbott immunoglobulin G (IgG), Genelabs IgG, and Walter Reed Army Institute of Research (WRAIR) IgM assays were about 90% sensitive; the Abbott IgG and WRAIR total Ig and IgM assays ...

journal_title：Journal of clinical microbiology

authors： Myint KS,Endy TP,Gibbons RV,Laras K,Mammen MP Jr,Sedyaningsih ER,Seriwatana J,Glass JS,Narupiti S,Corwin AL

更新日期：2006-04-01 00:00:00

abstract：:We evaluated a newly commercial enzyme immunoassay (EIA) (Biotest Legionella Urin Antigen EIA; Biotest AG, Dreieich, Germany) for detection of antigens of all Legionella pneumophila serogroups with a relatively wide spectrum of cross-reactivity as well as antigens of other Legionella spp. by comparing its sensitivity ...

journal_title：Journal of clinical microbiology

authors： Domínguez JA,Galí N,Pedroso P,Fargas A,Padilla E,Manterola JM,Matas L

更新日期：1998-09-01 00:00:00

abstract：:A VIM metallo-beta-lactamase-producing Aeromonas hydrophila strain carrying an integron-borne bla(VIM-4) gene was isolated from a cirrhotic patient's fecal sample in a Budapest hospital. The variable region of this integron is identical with that of a previously characterized integron from Pseudomonas aeruginosa clini...

journal_title：Journal of clinical microbiology

authors： Libisch B,Giske CG,Kovács B,Tóth TG,Füzi M

更新日期：2008-05-01 00:00:00

abstract：:Legionella is the causative agent for Legionnaires' disease (LD) and is responsible for several large outbreaks in the world. More than 90% of LD cases are caused by Legionella pneumophila, and studies on the origin and transmission routes of this pathogen rely on adequate molecular characterization of isolates. Curre...

journal_title：Journal of clinical microbiology

authors： Bosch T,Euser SM,Landman F,Bruin JP,IJzerman EP,den Boer JW,Schouls LM

更新日期：2015-10-01 00:00:00

abstract：:"Norwalk-like viruses" (NLVs) are the most common cause of outbreaks of nonbacterial gastroenteritis worldwide. To date, the method most widely used for typing of NLV strains is sequencing and subsequent phylogenetic analysis of reverse transcription (RT)-PCR products, which has revealed the existence of stable distin...

journal_title：Journal of clinical microbiology

authors： Vinjé J,Koopmans MP

更新日期：2000-07-01 00:00:00

abstract：:During a 1-year survey of Shiga toxin-producing Escherichia coli (STEC) prevalence in central France, 2,143 samples were investigated by PCR for Shiga toxin-encoding genes. A total of 330 (70%) of 471 fecal samples collected from healthy cattle at the Clermont-Ferrand slaughterhouse, 47 (11%) of 411 beef samples, 60 (...

journal_title：Journal of clinical microbiology

authors： Pradel N,Livrelli V,De Champs C,Palcoux JB,Reynaud A,Scheutz F,Sirot J,Joly B,Forestier C

更新日期：2000-03-01 00:00:00

abstract：:Mycobacteria include a large number of pathogens. Identification to species level is important for diagnoses and treatments. Here, we report the development of a Web-accessible database of the hsp65 locus sequences (http://msis.mycobacteria.info) from 149 out of 150 Mycobacterium species/subspecies. This database can ...

journal_title：Journal of clinical microbiology

authors： Dai J,Chen Y,Lauzardo M

更新日期：2011-06-01 00:00:00