当前位置： SCI文献检索 > JOURNAL OF CLINICAL MICROBIOLOGY期刊下所有文献 > Rapid and accurate identification of human isolates of Pasteurella and related species by sequencing the sodA gene.

Rapid and accurate identification of human isolates of Pasteurella and related species by sequencing the sodA gene.

Abstract：

:The identification of Pasteurella and related bacteria remains a challenge. Here, a 449- to 473-bp fragment (sodA(int)) internal to the sodA gene, encoding the manganese-dependent superoxide dismutase, was amplified and sequenced with a single pair of degenerate primers from the type strains of Pasteurella (18 strains), Gallibacterium (1 strain), and Mannheimia (5 strains) species. The sodA(int)-based phylogenetic tree was in general agreement with that inferred from the analysis of the corresponding 16S rRNA gene sequences, with members of the Pasteurella sensu stricto cluster (Pasteurella multocida, Pasteurella canis, Pasteurella dagmatis, and Pasteurella stomatis) forming a monophyletic group and Gallibacterium and Mannheimia being independent monophyletic genera. However, the sodA(int) sequences showed a markedly higher divergence than the corresponding 16S rRNA genes, confirming that sodA is a potent target to differentiate related species. Thirty-three independent human clinical isolates phenotypically assigned to 13 Pasteurella species by a reference laboratory were successfully identified by comparing their sodA(int) sequences to those of the type species. In the course of this work, we identified the first Gallibacterium anatis isolate ever reported from a human clinical specimen. The sodA(int) sequences of the clinical isolates displayed less than 2.5% divergence from those of the corresponding type strains, except for the Pasteurella pneumotropica isolates, which were closely related to each other (> 98% sodA(int) sequence identity) but shared only 92% sodA(int) identity with the type strain. The method described here provides a rapid and accurate tool for species identification of Pasteurella isolates when access to a sequencing facility is available.

journal_name

J Clin Microbiol

journal_title

Journal of clinical microbiology

authors

Gautier AL,Dubois D,Escande F,Avril JL,Trieu-Cuot P,Gaillot O

doi

10.1128/JCM.43.5.2307-2314.2005

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

2307-14

issue

eissn

0095-1137

issn

1098-660X

pii

43/5/2307

journal_volume

pub_type

杂志文章

在线工具

Rapid and accurate identification of human isolates of Pasteurella and related species by sequencing the sodA gene.