Comparison of Manual and Fully Automated AIX1000 Rapid Plasma Reagin Assays for Laboratory Diagnosis of Syphilis.

Abstract:

:The analytical performance of the AIX1000 system, a fully automated and recently FDA-cleared rapid plasma reagin (RPR) system, was evaluated by comparison to a manual RPR test in a traditional syphilis testing algorithm. A total of 1,028 consecutive serum samples submitted for syphilis testing to the University of North Carolina Hospitals Clinical Immunology Laboratory were tested per each manufacturer's instructions. Among those samples, 996 were nonreactive and 20 were reactive using both the ASI RPR card system and the AIX1000 system. Of the 12 discrepant specimens, 11 were AIX1000 reactive and ASI card nonreactive whereas 1 specimen was ASI card reactive and AIX1000 nonreactive. The sensitivity and specificity of the manual ASI card were 76.0% and 99.8%, respectively, while the sensitivity and specificity of the AIX100 were 100.0% and 99.4%, respectively (sensitivity P = 0.03). Among the 20 concordant reactive specimens, 68.4% of the titers agreed within ±1 dilution between methods. Reproducibility testing of the AIX1000 system demonstrated qualitative and semiquantitative (within ±1 dilution) agreement between specimens tested on different days of 96.0% and 76.0%, respectively, and 100.0% agreement between replicates within the same run. One of 24 samples analyzed under other disease conditions was reactive on both the AIX1000 system and the ASI card. Overall, the fully automated AIX1000 system demonstrated significantly enhanced sensitivity and specificity similar to that of the manual ASI RPR card test, making the AIX1000 system suitable for the laboratory diagnosis of syphilis in a clinical laboratory setting.

journal_name

J Clin Microbiol

authors

Sanfilippo AM,Freeman K,Schmitz JL

doi

10.1128/JCM.00214-18

subject

Has Abstract

pub_date

2018-07-26 00:00:00

issue

8

eissn

0095-1137

issn

1098-660X

pii

JCM.00214-18

journal_volume

56

pub_type

杂志文章
  • Environmental monitoring for gastroenteric viruses in a pediatric primary immunodeficiency unit.

    abstract::The aim of this study was to determine if gastroenteric viruses were present on surfaces and equipment in a pediatric primary immunodeficiency unit (PPIU) by environmental sampling using swabs and subsequent nucleic acid extraction and reverse transcriptase PCR assays. A PPIU was chosen, and 11 swabs were taken at the...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.44.2.395-399.2006

    authors: Gallimore CI,Taylor C,Gennery AR,Cant AJ,Galloway A,Iturriza-Gomara M,Gray JJ

    更新日期:2006-02-01 00:00:00

  • Carious dentine provides a habitat for a complex array of novel Prevotella-like bacteria.

    abstract::Previous analysis of the microbiology of advanced caries by culture and real-time PCR emphasized the high incidence and abundance of gram-negative anaerobic species, particularly Prevotella-like bacteria. The diversity of Prevotella-like bacteria was further explored by analyzing pooled bacterial DNA from lesions of c...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.42.11.5238-5244.2004

    authors: Nadkarni MA,Caldon CE,Chhour KL,Fisher IP,Martin FE,Jacques NA,Hunter N

    更新日期:2004-11-01 00:00:00

  • Racial tropism of a highly toxic clone of Actinobacillus actinomycetemcomitans associated with juvenile periodontitis.

    abstract::Actinobacillus actinomycetemcomitans strains with enhanced levels of production of leukotoxin are characterized by a 530-bp deletion from the promoter region of the leukotoxin gene operon. Previous isolates with this deletion constituted a single clone belonging to serotype b, although they displayed minor differences...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.35.12.3037-3042.1997

    authors: Haubek D,Dirienzo JM,Tinoco EM,Westergaard J,López NJ,Chung CP,Poulsen K,Kilian M

    更新日期:1997-12-01 00:00:00

  • Rapid automated method for screening of enteric pathogens from stool specimens.

    abstract::A total of 800 colonies suggestive of Salmonella, Shigella, or Yersinia species isolated on stool differential agar media were inoculated onto both conventional biochemical test media (triple sugar iron agar, urea agar, and phenylalanine agar) and Entero Pathogen Screen cards of the AutoMicrobic system (Vitek Systems,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.3.584-585.1987

    authors: Villasante PA,Agulla A,Merino FJ,Pérez T,Ladrón de Guevara C,Velasco AC

    更新日期:1987-03-01 00:00:00

  • Detection of bovine herpesvirus 1 glycoprotein E antibodies in individual milk samples by enzyme-linked immunosorbent assays.

    abstract::The purpose of this study was to determine whether individual milk samples can replace serum samples for the detection of bovine herpesvirus 1 (BHV1) glycoprotein E (gE)-specific antibodies. Serum and milk samples were collected at the same time from cattle in BHV1-free herds, cattle in unvaccinated herds, and cattle ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.2.409-413.1998

    authors: Wellenberg GJ,Verstraten ER,Mars MH,Van Oirschot JT

    更新日期:1998-02-01 00:00:00

  • Comparison of five PCR methods for detection of Helicobacter pylori DNA in gastric tissues.

    abstract::Five different PCR methods for the detection of Helicobacter pylori were evaluated. The results of this study indicate that of the five PCR methods examined, the ureC (glmM) gene PCR is the most sensitive and specific for the detection of H. pylori in gastric biopsy specimens. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.3.772-774.1999

    authors: Lu JJ,Perng CL,Shyu RY,Chen CH,Lou Q,Chong SK,Lee CH

    更新日期:1999-03-01 00:00:00

  • Isolation of Pseudomonas putrefaciens in intra-abdominal sepsis.

    abstract::We report the isolation of Pseudomonas putrefaciens from an intra-abdominal abscess in a patient with colonic carcinoma and from bile in two patients with biliary tract disease. In all three cases, P. putrefaciens was isolated in mixed culture with enteric bacteria. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.17.6.1173-1174.1983

    authors: Marne C,Pallarés R,Sitges-Serra A

    更新日期:1983-06-01 00:00:00

  • Ureaplasma urealyticum biovar specificity and diversity are encoded in multiple-banded antigen gene.

    abstract::Ureaplasma urealyticum is a commensal organism of the lower genital tract of females, but in a subpopulation of individuals, it can invade the upper genital tract. It is a significant cause of chorioamnionitis and neonatal morbidity and mortality. There are 14 recognized serovars of U. urealyticum; these can be divide...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.32.6.1464-1469.1994

    authors: Teng LJ,Zheng X,Glass JI,Watson HL,Tsai J,Cassell GH

    更新日期:1994-06-01 00:00:00

  • Escherichia hermannii as the sole isolate from a patient with purulent conjunctivitis.

    abstract::Escherichia hermannii was isolated in pure culture from a patient with acute purulent conjunctivitis after a minor ocular injury. This is the first report of E. hermannii isolated as the sole pathogen from an infected site without prior antibiotic exposure, confirming the pathogenic potential of the microorganism. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01119-08

    authors: Poulou A,Dimitroulia E,Markou F,Tsakris A

    更新日期:2008-11-01 00:00:00

  • Comparison of two enzyme immunoassays to culture for the diagnosis of chlamydial conjunctivitis and respiratory infections in infants.

    abstract::Data are limited for the performance of enzyme immunoassays for the detection of Chlamydia trachomatis in conjunctival and nasopharyngeal specimens from infants. The only available data are for one assay, Chlamydiazyme (Abbott Diagnostics). The purpose of this study was to compare a new enzyme immunoassay, Pathfinder ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.8.1725-1727.1990

    authors: Hammerschlag MR,Roblin PM,Gelling M,Worku M

    更新日期:1990-08-01 00:00:00

  • Direct immunofluorescence microscopy for rapid screening of Campylobacter enteritis.

    abstract::Diagnostic use of a direct fluorescent-antibody test for detection of Campylobacter jejuni and C. coli in human fecal specimens (n = 497) was compared with detection by culturing (specificity, 99.7%; sensitivity, 40%). Conjugates were prepared from immunoglobulin G antibody against 22 Lior C. jejuni and C. coli refere...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.24.5.863-865.1986

    authors: Hodge DS,Prescott JF,Shewen PE

    更新日期:1986-11-01 00:00:00

  • Screening of urine cultures by three automated systems.

    abstract::A study was conducted to compare three automated systems and the Gram stain for their ability to detect significant bacteriuria. A total of 1,000 urine specimens were evaluated by Autobac MTS (General Diagnostics), Auto Microbic system (AMS; Vitek Systems, Inc.), and MS-2 (Abbott Laboratories) and compared with a semi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.15.3.468-474.1982

    authors: Pezzlo MT,Tan GL,Peterson EM,de la Maza LM

    更新日期:1982-03-01 00:00:00

  • Development and application of real-time PCR assay for quantification of Mycobacterium ulcerans DNA.

    abstract::Buruli ulcer, an infection caused by Mycobacterium ulcerans, is, after tuberculosis and leprosy, the third most common mycobacterial disease. The mode of transmission of M. ulcerans is not exactly known, but since Buruli ulcer often occurs in focalized swampy areas, it is assumed that there is a reservoir of the patho...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.9.4231-4237.2003

    authors: Rondini S,Mensah-Quainoo E,Troll H,Bodmer T,Pluschke G

    更新日期:2003-09-01 00:00:00

  • Rapid detection of influenza virus by shell vial assay with monoclonal antibodies.

    abstract::Of 45 influenza virus strains (43 type A and 2 type B) detected in conventional tube cell cultures (average time, 4 days), 25 (56%) were detected by immunofluorescence in the shell vial assay 24 h postinoculation. The specific fluorescence produced should allow this procedure to be readily adapted by laboratories with...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.24.4.677-679.1986

    authors: Espy MJ,Smith TF,Harmon MW,Kendal AP

    更新日期:1986-10-01 00:00:00

  • Comparison of methods for extraction of nucleic acid from hemolytic serum for PCR amplification of hepatitis B virus DNA sequences.

    abstract::The sensitivity of PCR for the amplification of target nucleic acid sequences in clinical diagnostics may often be reduced due to the presence of inhibitory factors. Hemolytic serum contains a number of PCR inhibitors, one of which is hemin. In this study we have found that conventional methods of DNA extraction were ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.35.7.1897-1899.1997

    authors: Klein A,Barsuk R,Dagan S,Nusbaum O,Shouval D,Galun E

    更新日期:1997-07-01 00:00:00

  • Enhanced detection and typing of human papillomavirus (HPV) DNA in anogenital samples with PGMY primers and the Linear array HPV genotyping test.

    abstract::The Roche PGMY primer-based research prototype line blot assay (PGMY-LB) is a convenient tool in epidemiological studies for the detection and typing of human papillomavirus (HPV) DNA. This assay has been optimized and is being commercialized as the Linear Array HPV genotyping test (LA-HPV). We assessed the agreement ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00104-06

    authors: Coutlée F,Rouleau D,Petignat P,Ghattas G,Kornegay JR,Schlag P,Boyle S,Hankins C,Vézina S,Coté P,Macleod J,Voyer H,Forest P,Walmsley S,Canadian Women's HIV study Group.,Franco E

    更新日期:2006-06-01 00:00:00

  • Detection of the agent of heartwater, Cowdria ruminantium, in Amblyomma ticks by PCR: validation and application of the assay to field ticks.

    abstract::We have previously reported that the pCS20 PCR detection assay for Cowdria ruminantium, the causative agent of heartwater disease of ruminants, is more sensitive than xenodiagnosis and the pCS20 DNA probe for the detection of infection in the vector Amblyomma ticks. Here, we further assessed the reliability of the PCR...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.4.1539-1544.2000

    authors: Peter TF,Barbet AF,Alleman AR,Simbi BH,Burridge MJ,Mahan SM

    更新日期:2000-04-01 00:00:00

  • Normalized enzyme-linked immunosorbent assay for determining immunoglobulin G antibodies to cytomegalovirus.

    abstract::A normalized enzyme-linked immunosorbent assay for the determination of immunoglobulin G antibodies to cytomegalovirus is described. The rapid assay involves three 30-min incubations and permits the quantitation of antibody levels with a single-specimen dilution in conjunction with a reference antibody preparation. Th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.18.1.33-39.1983

    authors: Kiefer DJ,Phelps DA,Halbert SP

    更新日期:1983-07-01 00:00:00

  • Detection of bacteria in the presence of antibiotics by using specific monoclonal antibodies to neutralize the antibiotics.

    abstract::Inactivation of penicillin and gentamicin in cultures was achieved by using monoclonal antibodies against these antibiotics. A viridans group streptococcus (penicillin MIC, less than or equal to 0.06 micrograms/ml) and Escherichia coli ATCC 35218 (gentamicin MIC, less than or equal to 1 microgram/ml) were able to grow...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.9.1904-1906.1988

    authors: Sierra-Madero JG,Caulfield MJ,Hall GS,Washington JA

    更新日期:1988-09-01 00:00:00

  • Use of monoclonal antibodies to serotype Bordetella pertussis isolates: comparison of results obtained by indirect whole-cell enzyme-linked immunosorbent assay and bacterial microagglutination methods.

    abstract::Sixty-one Bordetella pertussis isolates were tested blindly in two laboratories to determine their serotype nature by monoclonal antibodies using two independent methods: the standard bacterial microagglutination assay and an indirect whole-cell enzyme-linked immunosorbent assay. Both methods gave concordant results i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.5.2449-2451.2005

    authors: Tsang RS,Sill ML,Advani A,Xing D,Newland P,Hallander H

    更新日期:2005-05-01 00:00:00

  • Clinical trial comparing bacitracin with Strep-A-Chek for accuracy and turnaround time in the presumptive identification of Streptococcus pyogenes.

    abstract::In a clinical trial, Strep-A-Chek (a 10-min chromogenic test) was compared with the bacitracin disk susceptibility test for accuracy and turnaround time in the presumptive identification of Streptococcus pyogenes. Among 461 isolates of beta-hemolytic streptococci (344 throat isolates and 117 isolates from other sites)...

    journal_title:Journal of clinical microbiology

    pub_type: 临床试验,杂志文章

    doi:10.1128/JCM.24.3.431-434.1986

    authors: Yajko DM,Lawrence J,Nassos P,Young J,Hadley WK

    更新日期:1986-09-01 00:00:00

  • Meningococcal Deduced Vaccine Antigen Reactivity (MenDeVAR) Index: a Rapid and Accessible Tool That Exploits Genomic Data in Public Health and Clinical Microbiology Applications.

    abstract::As microbial genomics makes increasingly important contributions to clinical and public health microbiology, the interpretation of whole-genome sequence data by nonspecialists becomes essential. In the absence of capsule-based vaccines, two protein-based vaccines have been used for the prevention of invasive serogroup...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02161-20

    authors: Rodrigues CMC,Jolley KA,Smith A,Cameron JC,Feavers IM,Maiden MCJ

    更新日期:2020-12-17 00:00:00

  • Serological studies of antigenic similarity between Japanese spotted fever rickettsiae and Weil-Felix test antigens.

    abstract::Acute and convalescent-phase sera obtained from 10 patients infected with a Japanese strain of spotted fever group (SFG) rickettsia were tested by the indirect immunoperoxidase test, the Weil-Felix test, an enzyme-linked immunosorbent assay (ELISA), and immunoblotting. By the Weil-Felix test, the reactivity of these s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.9.2441-2446.1992

    authors: Amano K,Hatakeyama H,Okuta M,Suto T,Mahara F

    更新日期:1992-09-01 00:00:00

  • Detection of Trichomonas vaginalis in vaginal specimens by direct immunofluorescence assay.

    abstract::Vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect Trichomonas vaginalis. The organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.24.6.1107-1108.1986

    authors: Smith RF

    更新日期:1986-12-01 00:00:00

  • Prevalence and Outcomes of Achromobacter Species Infections in Adults with Cystic Fibrosis: a North American Cohort Study.

    abstract::Achromobacter species are increasingly being detected in cystic fibrosis (CF) patients, with an unclear epidemiology and impact. We studied a cohort of patients attending a Canadian adult CF clinic who had positive sputum cultures for Achromobacter species in the period from 1984 to 2013. Infection was categorized as ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02556-16

    authors: Edwards BD,Greysson-Wong J,Somayaji R,Waddell B,Whelan FJ,Storey DG,Rabin HR,Surette MG,Parkins MD

    更新日期:2017-07-01 00:00:00

  • Assessment of Etest as an alternative to agar dilution for antimicrobial susceptibility testing of Neisseria gonorrhoeae.

    abstract::We studied whether the Etest can be used as an alternative to agar dilution to determine antimicrobial susceptibilities of ceftriaxone, cefixime, and cefpodoxime in Neisseria gonorrhoeae surveillance. One hundred fifteen clinical and laboratory isolates of N. gonorrhoeae were tested following the Clinical Laboratory I...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02131-13

    authors: Liu H,Taylor TH Jr,Pettus K,Trees D

    更新日期:2014-05-01 00:00:00

  • Comparison of PCR-ribotyping, arbitrarily primed PCR, and pulsed-field gel electrophoresis for typing Clostridium difficile.

    abstract::Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. Ho...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.7.2484-2487.2000

    authors: Bidet P,Lalande V,Salauze B,Burghoffer B,Avesani V,Delmée M,Rossier A,Barbut F,Petit JC

    更新日期:2000-07-01 00:00:00

  • Characterization of two DNA probes specific for Serpulina hyodysenteriae.

    abstract::Two DNA probes, one 1.1- and one 0.75-kb probe, specific for Serpulina hyodysenteriae were isolated from a genomic library generated from virulent S. hyodysenteriae 5380. These probes are highly specific and react with all S. hyodysenteriae strains tested. Under stringent conditions, the DNA probes did not react with ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.7.1746-1752.1993

    authors: Sotiropoulos C,Smith SC,Coloe PJ

    更新日期:1993-07-01 00:00:00

  • Detection of the microsporidian parasite Enterocytozoon bieneusi in specimens from patients with AIDS by PCR.

    abstract::Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.12.3230-3232.1996

    authors: Velásquez JN,Carnevale S,Guarnera EA,Labbé JH,Chertcoff A,Cabrera MG,Rodríguez MI

    更新日期:1996-12-01 00:00:00

  • Extremely high titers of serum antibodies against the streptococcal exoenzyme deoxyribonuclease B.

    abstract::In sera from 4 of 25,000 individuals tested for antibodies against streptococci, extremely high antideoxyribonuclease B titers ( > 10(6) U/ml) were found. Two of the cases were diagnosed as monoclonal gammopathies. The M-components were shown to possess the anti-deoxyribonuclease B activity. The other two cases were d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.6.720-723.1980

    authors: Hederstedt B,Holm SE,Norberg R

    更新日期:1980-06-01 00:00:00