Characterization of Neisseria elongata subsp. glycolytica isolates obtained from human wound specimens and blood cultures.

abstract：:Genotypic typing by restriction fragment length polymorphism and pulsed-field gel electrophoresis showed that two neonates in a neonatal ward were infected with the same Campylobacter jejuni strain. Isolates from the mother and brother of the index patient were identical to each other but distinct from the neonatal ty...

journal_title：Journal of clinical microbiology

authors： Llovo J,Mateo E,Muñoz A,Urquijo M,On SL,Fernández-Astorga A

更新日期：2003-08-01 00:00:00

abstract：:Brucellosis is a globally significant zoonosis, the control of which is difficult and resource intensive. Serological tests form a vital part of a multifactorial approach to control and are often performed in large numbers. The aim of the present study was to develop a new assay to improve the efficiency, ease, and ef...

journal_title：Journal of clinical microbiology

authors： McGiven JA,Thompson IJ,Commander NJ,Stack JA

更新日期：2009-10-01 00:00:00

abstract：:A survey was conducted among Spanish children with gastroenteritis treated in an emergency room. Reverse transcription-PCR with specimens negative for other enteric pathogens was used. The minimum incidence of human calicivirus infection was 7.7%, with Lordsdale as the predominant genotype. The clinical features and s...

journal_title：Journal of clinical microbiology

authors： Roman E,Negredo A,Dalton RM,Wilhelmi I,Sánchez-Fauquier A

更新日期：2002-10-01 00:00:00

abstract：:We compared examination of unstained and Gram-stained smears as methods of evaluating sputum quality. Of 100 slides examined, 96 (96%) were graded identically. Each discrepancy between methods was near the cutoff for specimen acceptability. We concluded that examination of unstained smears can provide an accurate, cos...

journal_title：Journal of clinical microbiology

authors： Wetterau LM,Zeimis RT,Hollick GE

更新日期：1986-07-01 00:00:00

abstract：:Although screening for maternal toxoplasmic seroconversion during pregnancy is based on immunodiagnostic assays, the diagnosis of clinically relevant toxoplasmosis greatly relies upon molecular methods. A problem is that this molecular diagnosis is subject to variation of performances, mainly due to a large diversity ...

journal_title：Journal of clinical microbiology

authors： Sterkers Y,Varlet-Marie E,Cassaing S,Brenier-Pinchart MP,Brun S,Dalle F,Delhaes L,Filisetti D,Pelloux H,Yera H,Bastien P

更新日期：2010-09-01 00:00:00

abstract：:Recently, three novel avian-origin swine influenza viruses (SIVs) were first isolated from pigs in Guangdong Province, southern China, yet little is known about the seroprevalence of avian influenza viruses among pigs in southern China. Here, we report for the first time the seroprevalence of avian H3, H4, and H6 infl...

journal_title：Journal of clinical microbiology

authors： Su S,Qi W,Chen J,Zhu W,Huang Z,Xie J,Zhang G

更新日期：2013-02-01 00:00:00

abstract：:We compared the coreceptor tropism-predicting performance of a specific genotypic algorithm for HIV-1 subtype D and that of the geno2pheno algorithm with different cutoffs. The D-specific algorithm and geno2pheno with a false-positivity rate cutoff of 2.5% had the same concordance with the phenotypic determination. Th...

journal_title：Journal of clinical microbiology

authors： Dina J,Raymond S,Maillard A,Le Guillou-Guillemette H,Rodalec A,Beby-Defaux A,Giraudeau G,Vallet S,Mourez T,Payan C,Vabret A,Ruffault A,Ferre V,Izopet J,Plantier JC

更新日期：2013-09-01 00:00:00

abstract：:The NucliSENS easyMAG automated system was compared to the column-based Qiagen method for Epstein-Barr virus (EBV) or cytomegalovirus (CMV) DNA extraction from whole blood before viral load determination using the corresponding R-gene amplification kits. Both extraction techniques exhibited a total agreement of 81.3% ...

journal_title：Journal of clinical microbiology

authors： Pillet S,Bourlet T,Pozzetto B

更新日期：2009-11-01 00:00:00

abstract：:Cryptococcus neoformans var. neoformans (serotype D) and C. neoformans var. grubii (serotype A) differ in geographic prevalence and dermatotropism, with C. neoformans var. neoformans strains being more prevalent among isolates from temperate countries as well as from skin infections. Analysis of 19 strains from each s...

journal_title：Journal of clinical microbiology

authors： Martinez LR,Garcia-Rivera J,Casadevall A

更新日期：2001-09-01 00:00:00

abstract：:The diagnostic value of RT-PCR of amniotic fluid (AF) for prenatal diagnosis of congenital rubella virus infection in 45 pregnant women with confirmed primary infection was assessed. Specificity of RT-PCR was 100% and sensitivity ranged between 83 and 95%. ...

journal_title：Journal of clinical microbiology

authors： Macé M,Cointe D,Six C,Levy-Bruhl D,Parent du Châtelet I,Ingrand D,Grangeot-Keros L

更新日期：2004-10-01 00:00:00

abstract：:In West African countries such as Ghana, efficient human immunodeficiency virus (HIV) testing is a priority in the fight against AIDS. A new immunochromatographic rapid test, Determine HIV-1/2 (Abbott Diagnostics, North Chicago, Ill.), that detects antibodies against HIV type 1 (HIV-1) and/or HIV-2 was evaluated using...

journal_title：Journal of clinical microbiology

authors： Aidoo S,Ampofo WK,Brandful JA,Nuvor SV,Ansah JK,Nii-Trebi N,Barnor JS,Apeagyei F,Sata T,Ofori-Adjei D,Ishikawa K

更新日期：2001-07-01 00:00:00

abstract：:Members of the genus Exophiala are often difficult to identify to the species level because of their variable morphological appearances. This paper describes the methods used to identify Exophiala mesophila and provides salient differential features for distinguishing other mesophilic members of the genus. ...

journal_title：Journal of clinical microbiology

authors： Porteous NB,Grooters AM,Redding SW,Thompson EH,Rinaldi MG,De Hoog GS,Sutton DA

更新日期：2003-08-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in com...

journal_title：Journal of clinical microbiology

authors： Christensen JJ,Dargis R,Hammer M,Justesen US,Nielsen XC,Kemp M,Danish MALDI-TOF MS Study Group.

更新日期：2012-05-01 00:00:00

abstract：:Nucleic acid amplification tests, such as PCR, are the method of choice for respiratory virus testing, due to their superior diagnostic accuracy and fast turnaround time. The Panther Fusion (Fusion; Hologic) system has an array of highly sensitive in vitro diagnostic (IVD) real-time PCR assays for respiratory viruses,...

journal_title：Journal of clinical microbiology

authors： Stellrecht KA,Cimino JL,Maceira VP

更新日期：2020-05-26 00:00:00

abstract：:An automated RNA isolation procedure on the Qiagen BioRobot is described for performing viral load tests for human immunodeficiency virus (HIV) with the Amplicor HIV type 1 test. The new procedure improves the precision of the assay and requires significantly less labor than the presently used manual RNA isolation pro...

journal_title：Journal of clinical microbiology

authors： Xu M,Chan Y,Fischer SH,Remaley AT

更新日期：2004-01-01 00:00:00

abstract：:A mass spectrometry (MS) method that detects a serum disaccharide (DS) (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this assay. Patients with the following IFI were selected according to the availability of sera obtained...

journal_title：Journal of clinical microbiology

authors： Cornu M,Sendid B,Mery A,François N,Mikulska M,Letscher-Bru V,De Carolis E,Damonti L,Titecat M,Bochud PY,Alanio A,Sanguinetti M,Viscoli C,Herbrecht R,Guerardel Y,Poulain D

更新日期：2019-04-26 00:00:00

abstract：:Multidrug-resistant (MDR) Klebsiella pneumoniae is one of the most important causes of nosocomial infections worldwide. After the spread of strains resistant to beta-lactams at the end of the previous century, the diffusion of isolates resistant to carbapenems and colistin is now reducing treatment options and the con...

journal_title：Journal of clinical microbiology

authors： Onori R,Gaiarsa S,Comandatore F,Pongolini S,Brisse S,Colombo A,Cassani G,Marone P,Grossi P,Minoja G,Bandi C,Sassera D,Toniolo A

更新日期：2015-09-01 00:00:00

abstract：:The sensitivities of MRC-5 and mink lung (ML) cells in centrifugation culture were compared simultaneously for the detection of cytomegalovirus (CMV) IE antigen (immediate-early antigen) from clinical specimens. Of 413 samples assayed, 51 (12%) were positive for CMV by both centrifugation and standard cell culture. At...

journal_title：Journal of clinical microbiology

authors： Gleaves CA,Hursh DA,Meyers JD

更新日期：1992-04-01 00:00:00

abstract：:Cefaclor is less stable than most cephalosporins in media at 35 degrees C. We demonstrated that the activity of cefaclor in Mueller-Hinton agar diminishes continuously at 4 degrees C, resulting in a loss of two-thirds of the activity within 21 days. We recommend that agar dilution plates for this cephalosporin be prep...

journal_title：Journal of clinical microbiology

authors： Surprenant AM,Preston DA

更新日期：1985-01-01 00:00:00

abstract：:A human astrovirus (HAstV) strain from Kenya was characterized by nucleotide sequence analysis. Sequences from open reading frame 1a (ORF1a) clustered with genotype 6/7, those from ORF1b clustered with genotype 3, and those from ORF2 clustered with genotype 2. A recombination point in the ORF1b-ORF2 junction was ident...

journal_title：Journal of clinical microbiology

authors： Wolfaardt M,Kiulia NM,Mwenda JM,Taylor MB

更新日期：2011-02-01 00:00:00

abstract：:A prospective study of Campylobacter jejuni infection was performed during a 6-month period in a cohort of 198 Chilean infants. Surveillance was based on biweekly home visits by a team of trained nurses. C. jejuni colonization was studied by culturing stool samples from diarrheal episodes and paired asymptomatic contr...

journal_title：Journal of clinical microbiology

authors： Figueroa G,Galeno H,Troncoso M,Toledo S,Soto V

更新日期：1989-05-01 00:00:00

abstract：:A method for the rapid detection of verotoxin-producing Escherichia coli in stool samples by PCR was evaluated. Verotoxin-1 and verotoxin-2 genes in DNA extracted directly from stool samples were amplified with oligonucleotide primers. Stools spiked with control organisms, E. coli C600 (H19B) (verotoxin-1) or E. coli ...

journal_title：Journal of clinical microbiology

authors： Ramotar K,Waldhart B,Church D,Szumski R,Louie TJ

更新日期：1995-03-01 00:00:00

abstract：:We studied whether the Etest can be used as an alternative to agar dilution to determine antimicrobial susceptibilities of ceftriaxone, cefixime, and cefpodoxime in Neisseria gonorrhoeae surveillance. One hundred fifteen clinical and laboratory isolates of N. gonorrhoeae were tested following the Clinical Laboratory I...

journal_title：Journal of clinical microbiology

authors： Liu H,Taylor TH Jr,Pettus K,Trees D

更新日期：2014-05-01 00:00:00

abstract：:New variants of the influenza A(H1N1)pdm09 and A(H3N2) viruses were detected in Taiwan between 2012 and 2013. Some of these variants were not detected in clinical specimens using a common real-time reverse transcription-PCR (RT-PCR) assay that targeted the conserved regions of the viral matrix (M) genes. An analysis o...

journal_title：Journal of clinical microbiology

authors： Yang JR,Kuo CY,Huang HY,Wu FT,Huang YL,Cheng CY,Su YT,Chang FY,Wu HS,Liu MT

更新日期：2014-01-01 00:00:00

abstract：:We have used a polymerase chain reaction (PCR) to examine cervical cancer biopsy specimens and pelvic lymph nodes for the presence of human papillomavirus type 16 (HPV 16) DNA. Of the 75 cervical specimens tested, 36 (48%) were positive for HPV 16 in the PCR. A total of 65 pelvic lymph nodes removed during radical sur...

journal_title：Journal of clinical microbiology

authors： Czeglédy J,Póka R,Veress G,Gergely L

更新日期：1992-01-01 00:00:00

abstract：:Two ambient-temperature, dry plasma transport systems, ViveST tubes and RNASound RNA sampling cards, and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery. Significant RNA loss occurred: ViveST+MiniMag > ViveST+QIAamp > RNASound+QIAamp. RNA loss and low specimen volumes may affect the sensit...

journal_title：Journal of clinical microbiology

authors： Levine M,Beck I,Styrchak S,Pepper G,Frenkel L

更新日期：2016-07-01 00:00:00

abstract：:We report a case of a 27-year-old male who presented with respiratory distress that required mechanical ventilation. Transbronchial biopsy revealed adiaspores of the fungus Emmonsia crescens within granulomata, a condition known as adiaspiromycosis. The patient received amphotericin products and corticosteroids, follo...

journal_title：Journal of clinical microbiology

authors： Anstead GM,Sutton DA,Graybill JR

更新日期：2012-04-01 00:00:00

abstract：:We developed a liquid bead microarray (LBMA) assay for genotyping genital human papillomaviruses (HPVs) based on the MY09-MY11-HMB01 PCR system and the reverse line blot (RLB) assay probe sequences. Using individual HPV plasmids, we were able to detect as few as 50 copies per reaction. In two separate retrospective st...

journal_title：Journal of clinical microbiology

authors： Feng Q,Cherne S,Winer RL,Balasubramanian A,Lee SK,Hawes SE,Kiviat NB,Koutsky LA

更新日期：2009-03-01 00:00:00

abstract：:To determine whether DNA detection of high-risk human papillomavirus could represent a reliable screening technique and a useful follow-up method, we investigated the performance of the Hybrid Capture II assay in detecting high-grade cervical lesions. The test positivity at 1.86 pg/ml had a high sensitivity (94.7%) an...

journal_title：Journal of clinical microbiology

authors： Lin HP,Huang YY,Wu HY,Kao JT

更新日期：2004-01-01 00:00:00

abstract：:The zygomycetous mold Cokeromyces recurvatus was isolated from the pleural and peritoneal fluids of a 64-year-old man with a history of peptic ulcer disease and alcohol abuse. The patient presented to the hospital with severe abdominal pain and a ruptured duodenal ulcer. This is one of the few times that C.recurvatus ...

journal_title：Journal of clinical microbiology

authors： Munipalli B,Rinaldi MG,Greenberg SB

更新日期：1996-10-01 00:00:00