Direct detection of verotoxin-producing Escherichia coli in stool samples by PCR.

abstract：:The food-borne pathogen Listeria monocytogenes is genetically heterogeneous. Although some clonal groups have been implicated in multiple outbreaks, there is currently no consensus on how "epidemic clones" should be defined. The objectives of this work were to compare the patterns of sequence diversity on two sets of ...

journal_title：Journal of clinical microbiology

authors： Cantinelli T,Chenal-Francisque V,Diancourt L,Frezal L,Leclercq A,Wirth T,Lecuit M,Brisse S

更新日期：2013-11-01 00:00:00

abstract：:Two group A, subgroup I, rotavirus strains possessing "super short" RNA patterns were adapted to growth in MA-104 cells. Both produced marked cytopathic effect in primary culture. Reciprocal cross-neutralization titers, polypeptide analysis, and the serum neutralizing antibody response of an infected child suggested t...

journal_title：Journal of clinical microbiology

authors： Albert MJ,Unicomb LE,Bishop RF

更新日期：1987-01-01 00:00:00

abstract：:The genomes of two novel human papillomavirus (HPV) types, HPV68 and HPV70, were cloned from a low-grade cervical intraepithelial neoplasia and a vulvar papilloma, respectively, and partially sequenced. Both types are related to HPV39, a potentially oncogenic virus. HPV68 and HPV70 were also detected in genital intrae...

journal_title：Journal of clinical microbiology

authors： Longuet M,Beaudenon S,Orth G

更新日期：1996-03-01 00:00:00

abstract：:The majority of existing human papillomavirus (HPV) genotyping assays are based on multiplex PCR using consensus or degenerate primers. We developed a Templex HPV assay that simultaneously detects and identifies 25 common HPV genotypes in a single-tube reaction using type-specific primers for the HPV-specific E6 and E...

journal_title：Journal of clinical microbiology

authors： Han J,Swan DC,Smith SJ,Lum SH,Sefers SE,Unger ER,Tang YW

更新日期：2006-11-01 00:00:00

abstract：:After repeated use of fluconazole for therapy of oropharyngeal candidosis, the emergence of in vitro fluconazole-resistant Candida albicans isolates (MIC, > or = 25 micrograms/ml) together with oral candidosis unresponsive to oral dosages of up to 400 mg of fluconazole were observed in patients with human immunodefici...

journal_title：Journal of clinical microbiology

authors： Ruhnke M,Eigler A,Tennagen I,Geiseler B,Engelmann E,Trautmann M

更新日期：1994-09-01 00:00:00

abstract：:A procedure for discrimination between scrapie and bovine spongiform encephalopathy (BSE) in sheep is of importance for establishing whether BSE has entered the sheep population. Since BSE has not yet been found in sheep at the farm level, such discrimination procedures can be developed only with experimental sheep BS...

journal_title：Journal of clinical microbiology

authors： Thuring CM,Erkens JH,Jacobs JG,Bossers A,Van Keulen LJ,Garssen GJ,Van Zijderveld FG,Ryder SJ,Groschup MH,Sweeney T,Langeveld JP

更新日期：2004-03-01 00:00:00

abstract：:Potomac horse fever, a recently recognized disease of equines, characterized by high fever, leukopenia, and a profuse diarrhea, was studied for its etiology. An Ehrlichia organism was isolated in equine macrophage-fibroblast cell cultures and mouse macrophage cell cultures from the mononuclear cells of blood of infect...

journal_title：Journal of clinical microbiology

authors： Dutta SK,Myrup AC,Rice RM,Robl MG,Hammond RC

更新日期：1985-08-01 00:00:00

abstract：:Survival of anaerobic and aerobic bacteria in a commercially available, non-supportive, gassed (oxygen-free) transport container (Anaport) was evaluated quantitatively. Saline-suspended obligate anaerobes survived significantly better in the gassed container in aerobic control tubes (P less than 0.025, t test), and co...

journal_title：Journal of clinical microbiology

authors： Chow AW,Cunningham PJ,Guze LB

更新日期：1976-02-01 00:00:00

abstract：:Strains of the Beijing genotype family of Mycobacterium tuberculosis are a cause of particular concern because of their increasing dissemination in the world and their association with drug resistance. Phylogenetically, this family includes distinct ancient and modern sublineages. The modern strains, contrary to the a...

journal_title：Journal of clinical microbiology

authors： Ribeiro SC,Gomes LL,Amaral EP,Andrade MR,Almeida FM,Rezende AL,Lanes VR,Carvalho EC,Suffys PN,Mokrousov I,Lasunskaia EB

更新日期：2014-07-01 00:00:00

abstract：:Combination therapy can successfully suppress human immunodeficiency virus (HIV) replication in patients but selects for drug resistance, requiring subsequent resistance-guided therapeutic changes. This report describes the development and validation of a novel assay that offers a uniform method to measure susceptibil...

journal_title：Journal of clinical microbiology

authors： Covens K,Dekeersmaeker N,Schrooten Y,Weber J,Schols D,Quiñones-Mateu ME,Vandamme AM,Van Laethem K

更新日期：2009-07-01 00:00:00

abstract：:Acanthamoeba keratitis (AK) is a rare but sight-threatening ocular infection. Outbreaks have been associated with contaminated water and contact lens wear. The epidemiology and pathology may be associated with unique genotypes. We determined the Rns genotype for 37 clinical isolates from 23 patients presenting at the ...

journal_title：Journal of clinical microbiology

authors： Ledee DR,Iovieno A,Miller D,Mandal N,Diaz M,Fell J,Fini ME,Alfonso EC

更新日期：2009-05-01 00:00:00

abstract：:Human papillomavirus (HPV) plays a key role in the development of cervical and laryngeal cancers. The aim of our study was to compare the performance of a new hydrogel-based HPV genotyping biochip assay (Biochip) to a commercially available and CE-marked conventional PCR followed by reverse hybridization (GenID-PCR). ...

journal_title：Journal of clinical microbiology

authors： Schenk T,Brandstetter T,Zur Hausen A,Alt-Mörbe J,Huzly D,Rühe J

更新日期：2009-05-01 00:00:00

abstract：:Four commercial kits, Oxi/Ferm (OF), API 20E (AP), Minitek (MT; BBL Microbiology Systems), and Flow N/F (NF), were evaluated, without additional tests, for identification of 258 gram-negative nonfermentative rods. OF and MT were read after 48 h of incubation, and AP and NF were read after both 24 and 48 h of incubatio...

journal_title：Journal of clinical microbiology

authors： Appelbaum PC,Stavitz J,Bentz MS,von Kuster LC

更新日期：1980-08-01 00:00:00

abstract：:The performance characteristics of the Gen-Probe Probe Competition Assay (PCA) used in conjunction with the Gen-Probe PACE 2 and 2C direct detection assays for Chlamydia trachomatis were examined. Data collected by five public health laboratories by using the Gen-Probe PACE 2 were pooled and analyzed. Of 25,081 endoce...

journal_title：Journal of clinical microbiology

authors： Beebe JL,Sharpton TR,Zanto SN,Steece RS,Rogers C,Mottice SL

更新日期：1997-02-01 00:00:00

abstract：:Sera from patients with paracoccidioidomycosis (PCM), histoplasmosis (HP), or Jorge Lobo's disease (JL) were titrated against purified gp43 from Paracoccidioides brasiliensis by using both enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation (IPP) reactions with 125I-labeled antigens. In IPP, PCM sera and...

journal_title：Journal of clinical microbiology

authors： Puccia R,Travassos LR

更新日期：1991-08-01 00:00:00

abstract：:Human (n = 33) and canine (n = 53) Campylobacter upsaliensis isolates from seven countries were genotyped by a new amplified fragment length polymorphism method. We observed 100% typeability and high overall diversity. The majority of human strains (23/33) clustered separately from canine strains, indicating that dogs...

journal_title：Journal of clinical microbiology

authors： Damborg P,Guardabassi L,Pedersen K,Kokotovic B

更新日期：2008-04-01 00:00:00

abstract：:The classification of viruses is relevant to a number of scientific and clinical disciplines, including the practice of diagnostic virology. Here, we provide an update to our previous review of taxonomic changes for disease-causing viruses in humans and vertebrate animals, covering changes between 2018 and 2020. Recen...

journal_title：Journal of clinical microbiology

authors： Loeffelholz MJ,Fenwick BW

更新日期：2021-01-21 00:00:00

abstract：:Swine influenza viruses (SIV) have been shown to sporadically infect humans and are infrequently identified by the Influenza Division of the Centers for Disease Control and Prevention (CDC) after being received as unsubtypeable influenza A virus samples. Real-time reverse transcriptase PCR (rRT-PCR) procedures for det...

journal_title：Journal of clinical microbiology

authors： Shu B,Wu KH,Emery S,Villanueva J,Johnson R,Guthrie E,Berman L,Warnes C,Barnes N,Klimov A,Lindstrom S

更新日期：2011-07-01 00:00:00

abstract：:We evaluated the Prodesse ProFlu-1 real-time reverse transcription-PCR multiplex assay with the SmartCycler instrument for the detection of human respiratory syncytial virus (RSV) and influenza A and B viruses in comparison to conventional cell culture and antigen immunoassays with the BD Directigen A+B and Binax NOW ...

journal_title：Journal of clinical microbiology

authors： Liao RS,Tomalty LL,Majury A,Zoutman DE

更新日期：2009-03-01 00:00:00

abstract：:We conducted a case-control study using the Fungitell assay, the novel Platelia Candida Antigen (Ag) Plus and Candida Antibody (Ab) Plus assays, and the Cand-Tec latex agglutination test to evaluate the usefulness of (1→3)-β-D-glucan (BDG), mannan antigen with/without anti-mannan antibody, and Cand-Tec Candida antigen...

journal_title：Journal of clinical microbiology

authors： Held J,Kohlberger I,Rappold E,Busse Grawitz A,Häcker G

更新日期：2013-04-01 00:00:00

abstract：:The ability of vancomycin resistance determinants to be horizontally transferred within enterococci species is a concern. Identification and characterization of vancomycin-resistant enterococci (VRE) in a clinical isolate have a significant impact on infection control practices. In this study, we describe a clinical i...

journal_title：Journal of clinical microbiology

authors： Eshaghi A,Shahinas D,Li A,Kariyawasam R,Banh P,Desjardins M,Melano RG,Patel SN

更新日期：2015-07-01 00:00:00

abstract：:Sera (920) were tested to evaluate the use of heated versus unheated sera in the hemagglutination treponemal test for syphilis. The heated and unheated samples were tested on the same day with the hemagglutination treponemal test for syphilis kit according to the manufacturer's protocol. Agreement of results between t...

journal_title：Journal of clinical microbiology

authors： Hambie EA,Larsen SA,Perryman MW,Pettit DE,Feeley JC

更新日期：1982-02-01 00:00:00

abstract：:Between September 1995 and August 1998, the incidence and diversity of the main human rotavirus genotypes (G1, G2, G3, and G4 and P[8], P[4], P[6], and P[9]) among Irish children were determined by using established and adapted reverse transcriptase PCR-based genotyping methods. From a total of 193 rotavirus-positive ...

journal_title：Journal of clinical microbiology

authors： O'Mahony J,Foley B,Morgan S,Morgan JG,Hill C

更新日期：1999-06-01 00:00:00

abstract：:Recent studies have resulted in the proposal of a new species, Aeromonas schubertii (mannitol, sucrose, and indole negative), formerly termed Enteric Group 501, on the basis of the study of seven strains isolated from the southeastern and southwestern United States and Puerto Rico. We have isolated two phenotypically ...

journal_title：Journal of clinical microbiology

authors： Carnahan AM,Marii MA,Fanning GR,Pass MA,Joseph SW

更新日期：1989-08-01 00:00:00

abstract：:A set of universal degenerate primers which amplified, by PCR, a 600-bp oligomer encoding a portion of the 60-kDa heat shock protein (HSP60) of both Staphylococcus aureus and Staphylococcus epidermidis were developed. However, when used as a DNA probe, the 600-bp PCR product generated from S. epidermidis failed to cro...

journal_title：Journal of clinical microbiology

authors： Goh SH,Potter S,Wood JO,Hemmingsen SM,Reynolds RP,Chow AW

更新日期：1996-04-01 00:00:00

abstract：:Previous studies have reported the increased sensitivity of PCR targeting AF146527 over that of PCR targeting the B1 gene for diagnosis of toxoplasmosis. The present study suggests that the AF146527 element was absent in 4.8% of human Toxoplasma gondii-positive samples tested. The data argue that the B1 gene may be th...

journal_title：Journal of clinical microbiology

authors： Wahab T,Edvinsson B,Palm D,Lindh J

更新日期：2010-02-01 00:00:00

abstract：:The ability of Staphylococcus aureus to clot plasma through conformational activation of prothrombin by staphylocoagulase is used to distinguish S. aureus from coagulase-negative staphylococci. We show that while the direct thrombin inhibitor dabigatran inhibits staphylocoagulase activity, the clinical use of dabigatr...

journal_title：Journal of clinical microbiology

authors： Vanassche T,Verhaegen J,Peetermans WE,Hoylaerts MF,Verhamme P

更新日期：2010-11-01 00:00:00

abstract：:We evaluated the Phoenix automated microbiology system (BD Diagnostic Systems, Sparks, MD) for the identification (ID) and antimicrobial susceptibility testing (AST) of challenge and clinical staphylococci and enterococci recovered from patients in a tertiary-care medical center. In total, 424 isolates were tested: 90...

journal_title：Journal of clinical microbiology

authors： Carroll KC,Borek AP,Burger C,Glanz B,Bhally H,Henciak S,Flayhart DC

更新日期：2006-06-01 00:00:00

abstract：:We sequenced homologous chromosomal loci from several North American and European isolates of the Lyme disease spirochete Borrelia burgdorferi, as well as from the relapsing fever spirochete Borrelia hermsii. Inter- and intraspecies sequence comparisons permitted the design of B. burgdorferi-specific polymerase chain ...

journal_title：Journal of clinical microbiology

authors： Rosa PA,Hogan D,Schwan TG

更新日期：1991-03-01 00:00:00

abstract：:We examined an antiserum agar method to study its reliability in screening Staphylococcus aureus strains for capsule production. The encapsulated S. aureus Smith diffuse strain was compared with its nonencapsulated variant, Smith compact, in CCY medium containing 0.5% NaCl and 5.0% Smith diffuse rabbit antiserum. A ha...

journal_title：Journal of clinical microbiology

authors： West TE,Apicella MA

更新日期：1984-08-01 00:00:00