Automated procedure for improving the RNA isolation step in viral load testing for human immunodeficiency virus.

abstract：:Direct tube coagulase testing for identification of Staphylococcus aureus from BACTEC culture broth showed a sensitivity, a specificity, and positive and negative predicative values of 34%, 100%, 100%, and 80.2% with 2 h of incubation and 65%, 98.7, 99.7%, and 88.6% with 4 h of incubation. Anaerobic blood culture cont...

journal_title：Journal of clinical microbiology

authors： Qian Q,Eichelberger K,Kirby JE

更新日期：2007-07-01 00:00:00

abstract：:A newly developed enzyme immunoassay (EIA) was used to detect the presence of pneumococcal teichoic and lipoteichoic acids in cerebrospinal fluid (CSF) from patients with Streptococcus pneumoniae meningitis who were being treated with antibiotics. All initial CSF samples, which on culture grew S. pneumoniae, were posi...

journal_title：Journal of clinical microbiology

authors： Stuertz K,Merx I,Eiffert H,Schmutzhard E,Mäder M,Nau R

更新日期：1998-08-01 00:00:00

abstract：:We report on a case of phaeohyphomycosis caused by Alternaria infectoria in a renal transplant recipient with pulmonary infiltrates and multiple skin lesions. Diagnosis was based on microscopy and culture of the skin lesions. Treatment consisted of a combination of surgical excision and systemic antifungal therapy, fi...

journal_title：Journal of clinical microbiology

authors： Halaby T,Boots H,Vermeulen A,van der Ven A,Beguin H,van Hooff H,Jacobs J

更新日期：2001-05-01 00:00:00

abstract：:Enterococcus hirae is a rare isolate in clinical specimens. We describe a case of native aortic-valve endocarditis that was caused by Enterococcus hirae in a 72-year-old man. This is the first reported case of endocarditis due to this organism. ...

journal_title：Journal of clinical microbiology

authors： Poyart C,Lambert T,Morand P,Abassade P,Quesne G,Baudouy Y,Trieu-Cuot P

更新日期：2002-07-01 00:00:00

abstract：:The toluidine red unheated serum test (TRUST) antigen, a macroscopic flocculation test antigen developed by Pettit et al. (J. Clin. Microbiol. 18:1141-1145, 1983) by modifying the color-coded antigen of Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974), was compared with the Venereal Disease Research Laboratory...

journal_title：Journal of clinical microbiology

authors： Parham CE,Pettit DE,Larsen SA,Hambie EA,Perryman MW,McGrew BE

更新日期：1984-09-01 00:00:00

abstract：:A rapidly fatal case of pulmonary tularemia in a 43-year-old man who was transferred to a tertiary care facility is presented. The microbiology laboratory and autopsy services were not notified of the clinical suspicion of tularemia by the service caring for the patient. Despite having a laboratory bioterrorism proced...

journal_title：Journal of clinical microbiology

authors： Shapiro DS,Schwartz DR

更新日期：2002-06-01 00:00:00

abstract：:A 44-year-old diabetic female presented to a hospital in Jamaica with thermal burns. Trichosporon asahii was isolated from facial wounds, sputum, and a meningeal swab. Dissemination of the fungus was demonstrated in stained histological sections of the meninges and a brain abscess at autopsy. Pure growth of the fungus...

journal_title：Journal of clinical microbiology

authors： Heslop OD,Nyi Nyi MP,Abbott SP,Rainford LE,Castle DM,Coard KC

更新日期：2011-12-01 00:00:00

abstract：:A method is described for the routine preparation of sputum samples for detection of Mycobacterium tuberculosis by using polymerase chain reaction amplification. Liquefaction of sputum samples with NaOH and subsequent removal of inhibitors of the polymerase reaction with a 50% sucrose centrifugation step (5 min) in a ...

journal_title：Journal of clinical microbiology

authors： Victor T,du Toit R,van Helden PD

更新日期：1992-06-01 00:00:00

abstract：:The RD2 region of the internalization-associated gene prtF1, which encodes the fibronectin-binding repeat domain type 2 of protein F1, plays a crucial role in the entry of group A streptococci (GAS) into epithelial cells. A molecular study of the variability of the RD2 region was carried out with 77 independent Italia...

journal_title：Journal of clinical microbiology

authors： Spinaci C,Magi G,Zampaloni C,Vitali LA,Paoletti C,Catania MR,Prenna M,Ferrante L,Ripa S,Varaldo PE,Facinelli B

更新日期：2004-02-01 00:00:00

abstract：:The availability of the Tropheryma whipplei genome offers the putative possibility of choosing logical DNA targets. We applied a PCR assay (targeting repeated sequences of T. whipplei) to samples from patients with Whipple's disease and to those from members of a control group. When compared to the results seen with r...

journal_title：Journal of clinical microbiology

authors： Fenollar F,Fournier PE,Robert C,Raoult D

更新日期：2004-01-01 00:00:00

abstract：:Rapid diagnostic testing reduces the turnaround time for pathogen identification in the clinical microbiology laboratory, but the impact on patient care and hospital costs is a matter of speculation. Patel et al. (J. Clin. Microbiol. 55:60-67, 2017, https://doi.org/10.1128/JCM.01452-16) investigate the impact of matri...

journal_title：Journal of clinical microbiology

authors： McElvania TeKippe E

更新日期：2016-12-28 00:00:00

abstract：:Periodic monitoring of antimicrobial susceptibility of Neisseria gonorrhoeae is essential for early detection of emergence of drug resistance. A total of 343 gonococcal strains isolated from high-risk and general populations in Bangladesh from 1997 to 1999 were studied. The MICs of penicillin, tetracycline, ciprofloxa...

journal_title：Journal of clinical microbiology

authors： Rahman M,Sultan Z,Monira S,Alam A,Nessa K,Islam S,Nahar S,Shama-A-Waris,Alam Khan S,Bogaerts J,Islam N,Albert J

更新日期：2002-06-01 00:00:00

abstract：:A semisolid medium (designated Serratia differentiation medium) containing L-arabinose, ornithine, and selective inhibitor was used to differentiate three clinically encountered Serratia species. The inhibitor, Irgasan DP-300, was incorporated to eliminate false-positive reactions from most remaining Enterobacteriacea...

journal_title：Journal of clinical microbiology

authors： Gibson S,Friedman H

更新日期：1978-03-01 00:00:00

abstract：:By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the sampl...

journal_title：Journal of clinical microbiology

authors： Osaki T,Taguchi H,Yamaguchi H,Kamiya S

更新日期：1998-01-01 00:00:00

abstract：:Nontuberculous mycobacteria (NTM) cause severe opportunistic infections and have a rising incidence in most settings. Rising diagnostic need must be met by national reference laboratories, which rely on Clinical and Laboratory Standards Institute (CLSI) guideline-approved manual readout of microtiter plates for antimi...

journal_title：Journal of clinical microbiology

authors： Rockland M,Ruth MM,Aalders N,Pennings L,Hoefsloot W,Wattenberg M,van Ingen J

更新日期：2019-03-28 00:00:00

abstract：:An inexpensive, rapid, and simple colorimetric test for detection of bacteriuria is described. This test does not require bacterial growth and has the marked advantage of being able to quantify bacteria, even when the organisms are present in the urine of bacteriuric patients who are being treated with antibiotics. Th...

journal_title：Journal of clinical microbiology

authors： Wallis C,Melnick JL,Longoria CJ

更新日期：1981-09-01 00:00:00

abstract：:Although rotavirus genotype P[6] is one of the three most common VP4 specificities associated with human infection, the relatively few sequence data available in public databases suggest that the genetic variability within P[6] might be presently unexplored. Thus far, two human P[6] lineages (M37-like and AU19-like) a...

journal_title：Journal of clinical microbiology

authors： Bányai K,Martella V,Jakab F,Melegh B,Szücs G

更新日期：2004-09-01 00:00:00

abstract：:Two DNA probes, one 1.1- and one 0.75-kb probe, specific for Serpulina hyodysenteriae were isolated from a genomic library generated from virulent S. hyodysenteriae 5380. These probes are highly specific and react with all S. hyodysenteriae strains tested. Under stringent conditions, the DNA probes did not react with ...

journal_title：Journal of clinical microbiology

authors： Sotiropoulos C,Smith SC,Coloe PJ

更新日期：1993-07-01 00:00:00

abstract：:A Campylobacter fetus subsp. venerealis-specific 5' Taq nuclease PCR assay using a 3' minor groove binder-DNA probe (TaqMan MGB) was developed based on a subspecies-specific fragment of unknown identity (S. Hum, K. Quinn, J. Brunner, and S. L. On, Aust. Vet. J. 75:827-831, 1997). The assay specifically detected four C...

journal_title：Journal of clinical microbiology

authors： McMillen L,Fordyce G,Doogan VJ,Lew AE

更新日期：2006-03-01 00:00:00

abstract：:Chronic hepatitis B virus (HBV) infection via perinatal transmission is common in the Asia-Pacific region, but related quasispecies (QS) characteristics are not yet defined. To investigate the homologous, full-length HBV QS after perinatal infection and their clinical implications, five pairs of mother-daughter patien...

journal_title：Journal of clinical microbiology

authors： Liu F,Yu DM,Huang SY,Yu JL,Zhang DH,Gong QM,Zhang XX

更新日期：2014-05-01 00:00:00

abstract：:In summary, it is clear that in vitro susceptibility testing can predict outcome in selected clinical situations. The clearest data are from the fluconazole-treated AIDS patients with oropharyngeal candidiasis. In this setting, the homogeneity of the underlying immune defect, combined with the ease of identification a...

journal_title：Journal of clinical microbiology

authors： Ghannoum MA,Rex JH,Galgiani JN

更新日期：1996-03-01 00:00:00

abstract：:The group B streptococcus (GBS) capsular polysaccharide (CPS) is an important virulence factor which is also used for GBS typing. There are 10 CPS types (Ia, Ib, and II to IX). GBS that do not phenotypically type are considered nontypeable. All genes required for CPS synthesis are found on the GBS cps operon, which co...

journal_title：Journal of clinical microbiology

authors： Alhhazmi A,Pandey A,Tyrrell GJ

更新日期：2017-09-01 00:00:00

abstract：:Determination of anti-hepatitis E virus (anti-HEV) antibodies is still enigmatic. There is no gold standard, and results obtained with different assays often diverge. Herein, five assays were compared for detection of anti-HEV IgM and IgG. Serum samples from 500 Swedish blood donors and 316 patients, of whom 136 had s...

journal_title：Journal of clinical microbiology

authors： Norder H,Karlsson M,Mellgren Å,Konar J,Sandberg E,Lasson A,Castedal M,Magnius L,Lagging M

更新日期：2016-03-01 00:00:00

abstract：:The source of human immunodeficiency virus type 1 (HIV-1) RNA in cerebrospinal fluid (CSF) during HIV-1 infection is uncertain. The sequence heterogeneity of HIV-1 RNA in simultaneous CSF and plasma samples was characterized for five patients at the baseline and during the first week of antiretroviral therapy by two c...

journal_title：Journal of clinical microbiology

authors： Tang YW,Huong JT,Lloyd RM Jr,Spearman P,Haas DW

更新日期：2000-12-01 00:00:00

abstract：:Development of resistance during therapy with cefamandole contributes to treatment failure. A simple cefoxitin disk test was recently described which detects a cefamandole-active inducible beta-lactamase not otherwise detectable with cefamandole as the inducer. A case of breakthrough Enterobacter bacteremia due to se...

journal_title：Journal of clinical microbiology

authors： Beckwith DG,Jahre JA

更新日期：1980-10-01 00:00:00

abstract：:Noroviruses (NVs) are important human pathogens that cause acute gastroenteritis. Genetically related animal enteric NVs have also been described, but there is no evidence of interspecies transmission of NVs. In this study we characterized antibody prevalence among domestic pigs by using recombinant capsid antigens of...

journal_title：Journal of clinical microbiology

authors： Farkas T,Nakajima S,Sugieda M,Deng X,Zhong W,Jiang X

更新日期：2005-02-01 00:00:00

abstract：:Four methods for the detection of Trichomonas vaginalis in vaginal secretions from 88 symptomatic patients were compared: wet-mount examination, Kupferberg liquid medium, Hirsch charcoal agar, and the Papanicolaou smear. Hirsch diphasic slants and Kupferberg medium did not significantly differ in sensitivity from dire...

journal_title：Journal of clinical microbiology

authors： Thomason JL,Gelbart SM,Sobun JF,Schulien MB,Hamilton PR

更新日期：1988-09-01 00:00:00

abstract：:A patient with a dual Salmonella infection is described. Salmonella group B was recovered from three blood culture sets but was not detected in seven stool cultures. Salmonella group C2 was isolated from three of seven stool cultures but was not recovered from blood cultures. Specific, non-cross-reactive antibodies to...

journal_title：Journal of clinical microbiology

authors： Papasian CJ,Bartholomew WR,Neter E,Amsterdam D

更新日期：1984-09-01 00:00:00

abstract：:VP1 sequences were determined for poliovirus type 1 isolates obtained over a 189-day period from a poliomyelitis patient with common variable immunodeficiency syndrome (a defect in antibody formation). The isolate from the first sample, taken 11 days after onset of paralysis, contained two poliovirus populations, diff...

journal_title：Journal of clinical microbiology

authors： Kew OM,Sutter RW,Nottay BK,McDonough MJ,Prevots DR,Quick L,Pallansch MA

更新日期：1998-10-01 00:00:00

abstract：:Direct heteroduplex analysis and a universal heteroduplex generator assay were performed to detect rifampin resistance rapidly in Turkish Mycobacterium tuberculosis isolates. Cross-resistance to rifapentine, rifabutin, and rifalazil was investigated. A relationship between specific mutations and resistance patterns, w...

journal_title：Journal of clinical microbiology

authors： Saribaş Z,Kocagöz T,Alp A,Günalp A

更新日期：2003-02-01 00:00:00