Use of genome selected repeated sequences increases the sensitivity of PCR detection of Tropheryma whipplei.

abstract：:Borrelia burgdorferi expresses a conserved, species-specific 39-kDa protein (P39) that can stimulate antibodies during human infection. To confirm that anti-P39 antibodies are produced consistently in animals exposed to infectious spirochetes, white-footed mice, Peromyscus leucopus, and laboratory white mice, Mus musc...

journal_title：Journal of clinical microbiology

authors： Simpson WJ,Burgdorfer W,Schrumpf ME,Karstens RH,Schwan TG

更新日期：1991-02-01 00:00:00

abstract：:Actinobacillus actinomycetemcomitans is an important pathogen in the etiology of severe periodontitis. For epidemiological studies on the prevalence of certain pathogenic clones and transmission of this bacterium, adequate typing methods are necessary. The purpose of this study was to compare six different typing meth...

journal_title：Journal of clinical microbiology

authors： van Steenbergen TJ,Bosch-Tijhof CJ,van Winkelhoff AJ,Gmür R,de Graaff J

更新日期：1994-11-01 00:00:00

abstract：:Type-specific antigen was purified from the supernatant of type III group B streptococcal cultures, tyrosylated, and bound to microtiter wells for an enzyme-linked immunosorbent assay. The immunological specificity of the antigen and the assay was shown by (i) reaction only with homologous unabsorbed rabbit sera and (...

journal_title：Journal of clinical microbiology

authors： Anthony BF,Concepcion NF,McGeary SA,Ward JI,Heiner DC,Shapshak P,Insel RA

更新日期：1982-08-01 00:00:00

abstract：:Nasopharyngeal secretions from adults and children were obtained in Stockholm, Sweden, for routine diagnosis of influenza A virus, influenza B virus, respiratory syncytial (RS) virus, parainfluenza type 3 virus, and adenovirus infections by demonstration of viral antigens directly in the specimens. The cells in nasoph...

journal_title：Journal of clinical microbiology

authors： Grandien M,Pettersson CA,Gardner PS,Linde A,Stanton A

更新日期：1985-11-01 00:00:00

abstract：:Methodologies for biochemical identification of mycobacteria isolated from clinical samples are still cumbersome, taking skilled technicians 3 to 6 weeks. We describe here a 2-h identification system for mycobacterial isolates belonging to the Mycobacterium tuberculosis complex using a DNA probe. After 30 min of hands...

journal_title：Journal of clinical microbiology

authors： Mabilat C,Desvarenne S,Panteix G,Machabert N,Bernillon MH,Guardiola G,Cros P

更新日期：1994-11-01 00:00:00

abstract：:A protein antigen with an apparent molecular weight (Mr) of 31,000 was isolated from 0.2 M glycine hydrochloride (pH 2.2) extracts of a typical human fecal isolate, Campylobacter jejuni VC74. The protein was purified to homogeneity on a preparative scale by immunoaffinity chromatography followed by molecular sieving w...

journal_title：Journal of clinical microbiology

authors： Dubreuil JD,Kostrzynska M,Logan SM,Harris LA,Austin JW,Trust TJ

更新日期：1990-06-01 00:00:00

abstract：:The heterogeneity and the increasing clinical importance of the Enterobacter cloacae complex have often been discussed. However, little is known about molecular factors causing pathogenicity within this nomenspecies. Here, we analyzed the genetic differences between an avirulent plant isolate and a pathogenic strain c...

journal_title：Journal of clinical microbiology

authors： Kremer AN,Hoffmann H

更新日期：2012-10-01 00:00:00

abstract：:We serotyped 222 Pasteurella multocida strains isolated from swine lungs at slaughter. Capsular serotypes A and D were determined by the hyaluronidase sensitivity and acriflavin agglutination tests, respectively. Somatic antigens were determined by gel diffusion against standard antisera. Capsular serotype A was found...

journal_title：Journal of clinical microbiology

authors： Pijoan C,Morrison RB,Hilley HD

更新日期：1983-06-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAEC) is an emerging enteric pathogen that causes acute and chronic diarrhea among children, human immunodeficiency virus-infected patients, and travelers to developing regions of the world. The pathogenesis of EAEC strains involves the production of biofilm. In this study, we deter...

journal_title：Journal of clinical microbiology

authors： Mohamed JA,Huang DB,Jiang ZD,DuPont HL,Nataro JP,Belkind-Gerson J,Okhuysen PC

更新日期：2007-01-01 00:00:00

abstract：:This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on...

journal_title：Journal of clinical microbiology

authors： Hans A,Gaudaire D,Manuguerra JC,Leon A,Gessain A,Laugier C,Berthet N,Zientara S

更新日期：2015-01-01 00:00:00

abstract：:On 24 August 2020, the Centers for Disease Control and Prevention (CDC) updated its website to highlight that asymptomatic individuals, even those with exposure to a COVID-19-positive contact, do not necessarily need to be tested unless they have medical conditions associated with increased risk of severe illness from...

journal_title：Journal of clinical microbiology

authors： Schuetz AN,Hemarajata P,Mehta N,Campbell S,Mitchell S,Palavecino E,Butler-Wu S,Miller MB

更新日期：2020-12-17 00:00:00

abstract：:Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aa] 362 to 412) and middle regi...

journal_title：Journal of clinical microbiology

authors： He Y,Zhou Y,Wu H,Kou Z,Liu S,Jiang S

更新日期：2004-11-01 00:00:00

abstract：:Parameters like zone reading, inoculum density, and plate streaking influence the precision and accuracy of disk diffusion antibiotic susceptibility testing (AST). While improved reading precision has been demonstrated using automated imaging systems, standardization of the inoculum and of plate streaking have not bee...

journal_title：Journal of clinical microbiology

authors： Hombach M,Maurer FP,Pfiffner T,Böttger EC,Furrer R

更新日期：2015-12-01 00:00:00

abstract：:Genetic determinants of the invasive phenotype of Shigella spp. and enteroinvasive Escherichia coli (EIEC), two common agents of bacillary dysentery, are encoded on large (180- to 210 kilobase), nonconjugative plasmids. Several plasmid-encoded antigens have been implicated as important bacterial ligands that mediate t...

journal_title：Journal of clinical microbiology

authors： Venkatesan M,Buysse JM,Vandendries E,Kopecko DJ

更新日期：1988-02-01 00:00:00

abstract：:By using primers based on the sequence of a species-specific antigen of Helicobacter pylori (P. O'Toole, S.M. Logan, M. Kostrzynska. T. Wadström, and T.J. Trust, J. Bacteriol. 173:505-513, 1991), a protocol was established for detection of this microorganism in gastric biopsy samples by the polymerase chain reaction (...

journal_title：Journal of clinical microbiology

authors： Hammar M,Tyszkiewicz T,Wadström T,O'Toole PW

更新日期：1992-01-01 00:00:00

abstract：:It has been shown by analysis with monoclonal and polyclonal antibodies that outer surface protein C (OspC) of Borrelia burgdorferi sensu lato is highly heterogeneous. To determine if the heterogeneity has a genetic basis, the genes of 18 different B. burgdorferi sensu lato strains have been amplified by PCR, cloned, ...

journal_title：Journal of clinical microbiology

authors： Jauris-Heipke S,Liegl G,Preac-Mursic V,Rössler D,Schwab E,Soutschek E,Will G,Wilske B

更新日期：1995-07-01 00:00:00

abstract：:This report presents the case of a patient with a massive pyelonephritis and a urosepsis caused by Streptococcus pneumoniae. This case is unusual as the focus was distant from the respiratory tract, the usual primary site of infection caused by this organism. No other primary site of infection was documented. ...

journal_title：Journal of clinical microbiology

authors： Dufke S,Kunze-Kronawitter H,Schubert S

更新日期：2004-09-01 00:00:00

abstract：:Species of dermatophytes are classified into three anamorphic (asexual) genera, Epidermophyton, Microsporum, and Trichophyton. Conventional methods used to identify dermatophytes are often lengthy and may be inconclusive because of atypical microscopic or colony morphology. Based on the internal transcribed spacer 1 (...

journal_title：Journal of clinical microbiology

authors： Li HC,Bouchara JP,Hsu MM,Barton R,Chang TC

更新日期：2007-10-01 00:00:00

abstract：:Herpes simplex virus (HSV) infections of the central nervous system (CNS) are associated with significant morbidity and mortality rates in children. This study assessed the impact of a direct HSV (dHSV) PCR assay on the time to result reporting and the duration of acyclovir therapy for children with signs and symptoms...

journal_title：Journal of clinical microbiology

authors： Van TT,Mongkolrattanothai K,Arevalo M,Lustestica M,Dien Bard J

更新日期：2017-05-01 00:00:00

abstract：:A total of 100 random clinical urine specimens were divided and used to compare Becton, Dickinson & Co. B-D Urine Culture Kit-treated urine (BDU) with refrigerated urine (RU). Each RU-BDU pair was cultured with the standard calibrated loop-plate method and also screened with the Abbott Laboratories MS-2 system at 0, 5...

journal_title：Journal of clinical microbiology

authors： Hubbard WA,Shalis PJ,McClatchey KD

更新日期：1983-02-01 00:00:00

abstract：:Neisseria gonorrhoeae is a common bacterial sexually transmitted infection (STI). Currently, there are limited data on the bacterial load in both men and women and on both genital and extragenital sites. Therefore, we quantified N. gonorrhoeae bacterial loads in a large population of women, heterosexual men, and men w...

journal_title：Journal of clinical microbiology

authors： van der Veer BMJW,Hoebe CJPA,Dukers-Muijrers NHTM,van Alphen LB,Wolffs PFG

更新日期：2020-10-21 00:00:00

abstract：:The long-chain fatty acids extracted from the whole cells of 12 clinically significant species of peptococci and peptostreptococci were characterized by gas-liquid chromatography. The resulting methylated fatty acid profiles (and some unidentified compounds) of 82 strains allowed the 12 species to be separated into fo...

journal_title：Journal of clinical microbiology

authors： Wells CL,Field CR

更新日期：1976-12-01 00:00:00

abstract：:A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems cons...

journal_title：Journal of clinical microbiology

authors： Suzuki N,Nakano Y,Yoshida A,Yamashita Y,Kiyoura Y

更新日期：2004-08-01 00:00:00

abstract：:The ability of antibody induced by vaccination with recombinant gp160 (rgp160) to bind to native and recombinant human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins was measured. Thirty-three HIV-1-seronegative healthy adult volunteers were injected four times with 40 or 80 micrograms of an HIV-1LAV env...

journal_title：Journal of clinical microbiology

authors： Gorse GJ,Frey SE,Newman FK,Belshe RB

更新日期：1992-10-01 00:00:00

abstract：:The protease phenotypes expressed by isolates of Pseudomonas aeruginosa from cystic fibrosis (CF) patients were evaluated. The majority of isolates tested produced elastase (65%) or alkaline protease (64%) or both. The mucoid phenotype expressed by many CF isolates of P. aeruginosa did not absolutely restrict the expr...

journal_title：Journal of clinical microbiology

authors： Jagger KS,Bahner DR,Warren RL

更新日期：1983-01-01 00:00:00

abstract：:A protocol was optimized for the isolation of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and colostrum, with parameters including chemical decontamination, antibiotics, and different culture media. This study demonstrates that the efficiency of MAP recovery from milk is highly dependent upon the cultu...

journal_title：Journal of clinical microbiology

authors： Bradner L,Robbe-Austerman S,Beitz DC,Stabel JR

更新日期：2013-05-01 00:00:00

abstract：:Trichophyton rubrum was easily differentiated from T. mentagrophytes by its ability to assimilate sorbitol with an API 20C AUX strip. One hundred percent of 36 T. rubrum strains and none of 147 T. mentagrophytes strains assimilated sorbitol. ...

journal_title：Journal of clinical microbiology

authors： Rezusta A,Rubio MC,Alejandre MC

更新日期：1991-01-01 00:00:00

abstract：:Monoclonal antibodies (MAb) were produced against the specific Bordetella pertussis antigen pertussis toxin (PT). In preliminary studies, one MAb (IB12) was selected and used in an enzyme-linked dot blot immunoassay to evaluate the ability of the method to detect known amounts of PT in control experiments and to test ...

journal_title：Journal of clinical microbiology

authors： Friedman RL,Paulaitis S,McMillan JW

更新日期：1989-11-01 00:00:00

abstract：:A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a speci...

journal_title：Journal of clinical microbiology

authors： Sullivan TJ,Patel P,Hutchinson A,Ethridge SF,Parker MM

更新日期：2011-10-01 00:00:00

abstract：:By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the sampl...

journal_title：Journal of clinical microbiology

authors： Osaki T,Taguchi H,Yamaguchi H,Kamiya S

更新日期：1998-01-01 00:00:00