Impact of a Rapid Herpes Simplex Virus PCR Assay on Duration of Acyclovir Therapy.

abstract：:We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalacti...

journal_title：Journal of clinical microbiology

authors： Creti R,Fabretti F,Orefici G,von Hunolstein C

更新日期：2004-03-01 00:00:00

abstract：:Fingerprinting by randomly amplified polymorphic DNA (RAPD) analysis was used to differentiate Scedosporium prolificans isolates. A total of 59 arbitrary primers were screened with six unrelated S. prolificans isolates, and a panel of 12 primers was selected. The 12 primers were then used to detect DNA polymorphisms a...

journal_title：Journal of clinical microbiology

authors： San Millán R,Quindós G,Garaizar J,Salesa R,Guarro J,Pontón J

更新日期：1997-09-01 00:00:00

abstract：:Standard repetitive extragenic palindromic (REP)-PCR, enterobacterial repetitive intergenic consensus-PCR, and Salmonella enteritidis repetitive element-PCR methods for bacterial strain typing were performed with DNA extracted by boiling members of each of the currently recognized species of human viridans group strep...

journal_title：Journal of clinical microbiology

authors： Alam S,Brailsford SR,Whiley RA,Beighton D

更新日期：1999-09-01 00:00:00

abstract：:Freshly isolated clinical strains of Klebsiella were tested for the ability to fix dinitrogen by the acetylene reduction assay. Ability to detect this trait was markedly affected by cultural conditions. When the test was run at 37 degrees C in the presence of yeast extract (50 mg/liter), only 1.6% of the organisms wer...

journal_title：Journal of clinical microbiology

authors： Chambers CA,Silver WS

更新日期：1977-11-01 00:00:00

abstract：:The currently available diagnostics for Clostridium difficile infection (CDI) have major limitations. Despite mounting evidence that toxin detection is paramount for diagnosis, conventional toxin immunoassays are insufficiently sensitive and cytotoxicity assays too complex; assays that detect toxigenic organisms (toxi...

journal_title：Journal of clinical microbiology

authors： Song L,Zhao M,Duffy DC,Hansen J,Shields K,Wungjiranirun M,Chen X,Xu H,Leffler DA,Sambol SP,Gerding DN,Kelly CP,Pollock NR

更新日期：2015-10-01 00:00:00

abstract：:The IDI-MRSA assay (Infectio Diagnostic, Inc., Sainte-Foy, Quebec, Canada) with the Smart Cycler II rapid DNA amplification system (Cepheid, Sunnyvale, CA) appears to be sensitive and specific for the rapid detection of nasal colonization by methicillin-resistant Staphylococcus aureus (MRSA). We assessed the sensitivi...

journal_title：Journal of clinical microbiology

authors： Bishop EJ,Grabsch EA,Ballard SA,Mayall B,Xie S,Martin R,Grayson ML

更新日期：2006-08-01 00:00:00

abstract：:Potomac horse fever is an acute systemic equine disease caused by Ehrlichia risticii. Currently, serologic methods are widely used to diagnose this disease. However, serologic methods cannot determine whether the horse is presently infected or has been exposed to ehrlichial antigens in the past. The purpose of the pre...

journal_title：Journal of clinical microbiology

authors： Mott J,Rikihisa Y,Zhang Y,Reed SM,Yu CY

更新日期：1997-09-01 00:00:00

abstract：:By use of the nearly perfectly colinear genomes of Rickettsia conorii and Rickettsia prowazekii, we compared the usefulness of three types of sequences for typing of R. conorii isolates: (i) 5 variable coding genes comprising the 16S ribosomal DNA, gltA, ompB, and sca4 (gene D) genes, which are present in both genomes...

journal_title：Journal of clinical microbiology

authors： Fournier PE,Zhu Y,Ogata H,Raoult D

更新日期：2004-12-01 00:00:00

abstract：:Many parts of the Salmonella rfb gene clusters which are responsible for biosynthesis of the oligosaccharide-repeating units of the O-antigenic lipopolysaccharide have recently been cloned and sequenced. On the basis of this knowledge, three sets of nucleotide primers were selected to target defined regions of the abe...

journal_title：Journal of clinical microbiology

authors： Luk JM,Kongmuang U,Reeves PR,Lindberg AA

更新日期：1993-08-01 00:00:00

abstract：:Clostridium hathewayi is a newly described Clostridium species isolated from the feces of healthy human individuals, but its clinical significance is not known. We describe a case of human infection associated with C. hathewayi. The bacterium (strain HKU18) was isolated from the blood culture of a 39-year-old patient ...

journal_title：Journal of clinical microbiology

authors： Woo PC,Lau SK,Woo GK,Fung AM,Yiu VP,Yuen KY

更新日期：2004-12-01 00:00:00

abstract：:A total of 46 Borrelia burgdorferi sensu lato isolates that were isolated from patients with Lyme borreliosis and infected animals or were extracted from ticks of the genus Ixodes were analyzed. Large restriction fragment patterns obtained after cleavage of genomic DNAs with MluI were analyzed by pulsed-field gel elec...

journal_title：Journal of clinical microbiology

authors： Belfaiza J,Postic D,Bellenger E,Baranton G,Girons IS

更新日期：1993-11-01 00:00:00

abstract：:We compared the MicroScan Pos MIC Type 6 panel and AMS-Vitek Gram Positive Susceptibility Card (GPS-TA) to agar dilution screen plates for the detection of high-level aminoglycoside resistance in 182 enterococcal isolates. The specificity of the two commercial systems was 100%, with the exception of one susceptible is...

journal_title：Journal of clinical microbiology

authors： Szeto S,Louie M,Low DE,Patel M,Simor AE

更新日期：1991-06-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) genome is a linear double-stranded DNA of 152 kpb. It is divided into long and short regions of unique sequences termed U(L) and U(S), respectively, and these are flanked by regions of inverted internal and terminal repeats. Microsatellites are short tandem repeats of 1- to 6-nu...

journal_title：Journal of clinical microbiology

authors： Deback C,Boutolleau D,Depienne C,Luyt CE,Bonnafous P,Gautheret-Dejean A,Garrigue I,Agut H

更新日期：2009-03-01 00:00:00

abstract：:The babA2 and cagA genes were investigated in 208 Brazilian Helicobacter pylori strains. A strong association between babA2 and duodenal ulcer or gastric carcinoma was observed, even after adjusting for confounding factors, such as age, gender, and cagA status. cagA-positive strains were also independently associated ...

journal_title：Journal of clinical microbiology

authors： Oliveira AG,Santos A,Guerra JB,Rocha GA,Rocha AM,Oliveira CA,Cabral MM,Nogueira AM,Queiroz DM

更新日期：2003-08-01 00:00:00

abstract：:A methodologic study was performed to compare the polymerase chain reaction (PCR) and Southern blot hybridization, two commonly used testing strategies for the detection of human papillomavirus (HPV) infection. Three laboratories tested masked aliquots of exfoliated cervical cell specimens obtained from 120 women by c...

journal_title：Journal of clinical microbiology

authors： Schiffman MH,Bauer HM,Lorincz AT,Manos MM,Byrne JC,Glass AG,Cadell DM,Howley PM

更新日期：1991-03-01 00:00:00

abstract：:The mainstay of laboratory diagnosis for Lyme disease is two-tiered serological testing, in which a reactive first-tier enzyme-linked immunosorbent assay (ELISA) or an immunofluorescence assay is supplemented by separate IgM and IgG immunoblots. Recent data suggest that the C6 ELISA can be substituted for immunoblots ...

journal_title：Journal of clinical microbiology

authors： Wormser GP,Levin A,Soman S,Adenikinju O,Longo MV,Branda JA

更新日期：2013-12-01 00:00:00

abstract：:Several unusual fatty acids characterized strains of Francisella tularensis. Long-chain (C20-C26) acids and the hydroxy acids 2-hydroxy-decanoate, 3-hydroxy-hexadecanoate, and 3-hydroxy-octadecanoate appeared to be of special diagnostic value. ...

journal_title：Journal of clinical microbiology

authors： Jantzen E,Berdal BP,Omland T

更新日期：1979-12-01 00:00:00

abstract：:Molecular genotyping of strains of Trichophyton rubrum and T. mentagrophytes from patients with onychomycosis of the toes was performed to ascertain whether the fungal genotype changes over the course of time as sequential samples were obtained from patients receiving antifungal therapy and during follow-up. Sixty-six...

journal_title：Journal of clinical microbiology

authors： Gupta AK,Kohli Y,Summerbell RC

更新日期：2001-09-01 00:00:00

abstract：:The results of PCR-based molecular typing of Helicobacter pylori strains by restriction fragment length polymorphism analysis of a 1, 161-bp nucleotide sequence of the midregion of the vacA gene are reported. A total of 48 H. pylori strains isolated from gastric biopsy specimens obtained from 18 patients with peptic u...

journal_title：Journal of clinical microbiology

authors： Donati M,Storni E,D'Apote L,Moreno S,Tucci A,Poli L,Cevenini R

更新日期：1999-04-01 00:00:00

abstract：:The DNA relatedness and restriction fragment length polymorphism patterns of whole-cell DNA and the general phenotypic properties of 14 isolates of the form species Candida parapsilosis representing three diverse genetic groups were compared. The data confirm the unrelatedness of the three groups at the species level....

journal_title：Journal of clinical microbiology

authors： Roy B,Meyer SA

更新日期：1998-01-01 00:00:00

abstract：:Vibrio vulnificus was isolated from a bacteremic patient. This strain, together with other isolates of V. vulnificus, was compared with V. alginolyticus, V. fluvialis, and V. parahaemolyticus with regard to growth characteristics on enteric agar media (enabling isolation and identification) and production of exoenzyme...

journal_title：Journal of clinical microbiology

authors： Desmond EP,Janda JM,Adams FI,Bottone EJ

更新日期：1984-02-01 00:00:00

abstract：:Southern blot hybridization with the Ca3 probe and the C fragment of the Ca3 probe was used to assess the genetic relatedness of Candida albicans strains from one patient with recurrent C. albicans infection in whom the same strain was maintained, one patient in whom the infecting strain was replaced, and their male s...

journal_title：Journal of clinical microbiology

authors： Schröppel K,Rotman M,Galask R,Mac K,Soll DR

更新日期：1994-11-01 00:00:00

abstract：:Pneumococcal surface protein A (PspA) has been considered a potential candidate for human vaccines because of its serotype-independent protective immunity. Nasopharyngeal (NP) pneumococcal colonization is highly prevalent in infants and precedes the invasive disease. Thus, prevention of NP colonization may reduce the ...

journal_title：Journal of clinical microbiology

authors： Pimenta FC,Ribeiro-Dias F,Brandileone MC,Miyaji EN,Leite LC,Sgambatti de Andrade AL

更新日期：2006-08-01 00:00:00

abstract：:High-risk human papillomavirus (hrHPV) testing is now being introduced as a potential primary screening test for improved detection of cervical precancer and cancer. Current U.S. Food and Drug Administration-approved tests are batch tests that take several hours to complete. A rapid, non-batch test might permit point-...

journal_title：Journal of clinical microbiology

authors： Einstein MH,Smith KM,Davis TE,Schmeler KM,Ferris DG,Savage AH,Gray JE,Stoler MH,Wright TC Jr,Ferenczy A,Castle PE

更新日期：2014-06-01 00:00:00

abstract：:Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussi...

journal_title：Journal of clinical microbiology

authors： Vielemeyer O,Crouch JY,Edberg SC,Howe JG

更新日期：2004-02-01 00:00:00

abstract：:Rapid and reliable detection of methicillin-resistant Staphylococcus aureus (MRSA) carriers is crucial for the effective control of MRSA transmission in healthcare facilities. The aim of this study was to verify the performance of the IDI-MRSA real-time PCR assay for direct MRSA detection in diverse mucocutaneous swab...

journal_title：Journal of clinical microbiology

authors： de San N,Denis O,Gasasira MF,De Mendonça R,Nonhoff C,Struelens MJ

更新日期：2007-04-01 00:00:00

abstract：:A microhematocrit concentration method (MH) for immediate diagnosis of Chagas' disease during the acute stage or in congenital cases was standardized. Parasitemia as low as 1,000 parasites per ml was detected, after centrifugation of six 50-microliters capillary tubes, by 10-min microscopic observation of each buffy c...

journal_title：Journal of clinical microbiology

authors： Feilij H,Muller L,Gonzalez Cappa SM

更新日期：1983-08-01 00:00:00

abstract：:The carbohydrate fermentation test in cystine trypticase agar (BBL)-tubed medium was compared with a simple method using commercially available carbohydrate impregnated disks on culture inoculated Thayer-Martin medium with and without (vancomycin, colistimethate, and nystatin) inhibitors. There was 100% agreement betw...

journal_title：Journal of clinical microbiology

authors： Valu JA

更新日期：1976-02-01 00:00:00

abstract：:Between 1991 and 1995, an apparent high rate of Staphylococcus warneri bacteremias at the Royal Children's Hospital, Melbourne, Victoria, Australia, raised the possibility of a virulent nosocomial strain. In a retrospective review of 30 S. warneri bacteremias in children, organisms were viable and verified in 22 episo...

journal_title：Journal of clinical microbiology

authors： Buttery JP,Easton M,Pearson SR,Hogg GG

更新日期：1997-08-01 00:00:00

abstract：:Achromobacter species are increasingly being detected in cystic fibrosis (CF) patients, with an unclear epidemiology and impact. We studied a cohort of patients attending a Canadian adult CF clinic who had positive sputum cultures for Achromobacter species in the period from 1984 to 2013. Infection was categorized as ...

journal_title：Journal of clinical microbiology

authors： Edwards BD,Greysson-Wong J,Somayaji R,Waddell B,Whelan FJ,Storey DG,Rabin HR,Surette MG,Parkins MD

更新日期：2017-07-01 00:00:00