Abstract：
:The protease phenotypes expressed by isolates of Pseudomonas aeruginosa from cystic fibrosis (CF) patients were evaluated. The majority of isolates tested produced elastase (65%) or alkaline protease (64%) or both. The mucoid phenotype expressed by many CF isolates of P. aeruginosa did not absolutely restrict the expression of protease activity, although a higher percentage of nonmucoid isolates was proteolytic. When isolates from CF patients chronically infected with P. aeruginosa were compared to isolates from CF patients colonized with this organism, both groups were found to contain comparable percentages of elastase-producing strains and mucoid strains. However, the group of isolates from colonized patients contained a higher percentage of strains producing alkaline protease and expressing general protease activity. In addition, the group of isolates from chronically infected patients contained more weakly proteolytic isolates than either the group from colonized CF patients or a group of isolates from pediatric patients without CF. These data suggest that protease production may be important in the initial colonization of the respiratory tract of CF patients by P. aeruginosa.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Jagger KS,Bahner DR,Warren RLdoi
10.1128/JCM.17.1.55-59.1983subject
Has Abstractpub_date
1983-01-01 00:00:00pages
55-9issue
1eissn
0095-1137issn
1098-660Xjournal_volume
17pub_type
杂志文章abstract：:Automation of the clinical microbiology laboratory has become more prominent as laboratories face higher specimen volumes and understaffing and are becoming more consolidated. One recent advancement is the use of digital image analysis to rapidly distinguish between chromogenic growth for screening bacterial cultures....
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.01683-19
更新日期：2020-03-25 00:00:00
abstract：:The relationship of coagulase-negative staphylococcal slime production and adherence with the development and outcome of bloodstream infections in two Finnish hospitals was evaluated. Analysis of 64 strains from 62 adult septicemias disclosed 34 (53%) adherent slime producers. In comparison, only 142 (29%) of 489 sing...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.28.12.2779-2785.1990
更新日期：1990-12-01 00:00:00
abstract：:Although substantial epidemiologic evidence links Streptococcus mutans to caries, the pathobiology of caries may involve more complex communities of bacterial species. Molecular methods for bacterial identification and enumeration now make it possible to more precisely study the microbiota associated with dental carie...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.40.3.1001-1009.2002
更新日期：2002-03-01 00:00:00
abstract：:Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with c...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02206-16
更新日期：2017-03-01 00:00:00
abstract：:We retrospectively analyzed epidemiologic information associated with 22 cultures of Neisseria elongata subsp. nitroreducens (formerly CDC group M-6) submitted to the Microbial Diseases Laboratory, California Department of Health Services, Berkeley, over a 16-year period. The most common illnesses noted with this bact...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.30.3.719-720.1992
更新日期：1992-03-01 00:00:00
abstract：:Post kala-azar dermal leishmaniasis (PKDL) is a skin manifestation that usually develops after treatment of visceral leishmaniasis (VL), a major public health problem in India. The diagnosis and management of PKDL is complex. This is the first case report from India in which PKDL occurred after paromomycin treatment f...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.05966-11
更新日期：2012-04-01 00:00:00
abstract：:The increasing risk of Rift Valley fever virus (RVFV) infection as a global veterinary and public health threat demands the development of safe and accurate diagnostic tests. The aim of this study was to assess the suitability of a baculovirus expression system to produce recombinant RVFV nucleoprotein (N) for use as ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.01058-19
更新日期：2019-09-24 00:00:00
abstract：:Human cytomegalovirus (HCMV) strains display genetic polymorphisms, and these polymorphisms can be analyzed to study viral transmission and pathogenesis. Recently, short tandem repeat (STR) length polymorphisms have been identified in the HCMV genome. We assessed the utility of STRs in characterizing HCMV strains and ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.39.6.2219-2226.2001
更新日期：2001-06-01 00:00:00
abstract：:The role of atypical enteropathogenic Escherichia coli (EPEC) in childhood diarrhea is controversial. The aim of the present study was to search for genes linked with diarrhea in atypical EPEC strains from a case-control study among Norwegian children. Using DNA microarray analysis, genomic DNAs from strains isolated ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00429-06
更新日期：2006-10-01 00:00:00
abstract：:Sixteen metabolically deficient dwarf-colony mutants of Escherichia coli were isolated from urine culture and represented about 2% of all E. coli isolated during the same period. In 14 cases, mutants were isolated from debilitated patients: elderly persons or patients in the terminal stages of a chronic disease. In 15...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1978-12-01 00:00:00
abstract：:Two ambient-temperature, dry plasma transport systems, ViveST tubes and RNASound RNA sampling cards, and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery. Significant RNA loss occurred: ViveST+MiniMag > ViveST+QIAamp > RNASound+QIAamp. RNA loss and low specimen volumes may affect the sensit...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00541-16
更新日期：2016-07-01 00:00:00
abstract：:A reverse transcription-seminested PCR (RT-snPCR) assay was developed for the detection and identification of enterovirus (EV) RNA in clinical specimens. Three conserved protein motifs were identified by aligning the VP3 and VP1 sequences of prototype EV strains. Consensus degenerate primers were designed from a conse...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00542-06
更新日期：2006-08-01 00:00:00
abstract：:In 271 Enterobacter blood culture isolates from 12 hospitals, extended-spectrum beta-lactamase (ESBL) prevalence varied between 0% and 30% per hospital. High prevalence was associated with dissemination, indicating the potential relevance of infection control measures. Screening with cefepime or Vitek 2, followed by a...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00864-11
更新日期：2011-07-01 00:00:00
abstract：:A total of 215 typical strains of Candida albicans were studied for their ability to assimilate 11 carbon compounds. All isolates assimilated lactic acid, ribitol, succinic acid, methyl alpha-D-glucopyranoside. None of the isolates assimilated cellobiose and salicin; 1.9% of the isolates assimilated L-arabinose. Citri...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.13.1.163-166.1981
更新日期：1981-01-01 00:00:00
abstract：:The development of a satisfactory means to reliably distinguish between the two closely related species Candida albicans and Candida dubliniensis in the clinical mycology laboratory has proved difficult because these two species are phenotypically so similar. In this study, we have detected homologues of the pH-regula...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.37.5.1587-1590.1999
更新日期：1999-05-01 00:00:00
abstract：:The elimination of leprosy continues to be a challenge, with the disease remaining endemic in several countries. India accounts for the highest number of cases, and the identification of child cases indicates recent transmission. Genetic markers, like variable-number tandem repeats (VNTRs) and single-nucleotide polymo...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.05315-11
更新日期：2012-04-01 00:00:00
abstract：:A simple method of storage and retrieval of serum samples for adenovirus serology is described. Complement-fixing antibodies remained stable for at least 5 years when stored at -10 degrees C or below. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1977-02-01 00:00:00
abstract：:A glycoprotein from bovine erythrocyte membrane was evaluated in two immunoassays as a reagent for the serodiagnosis of infectious mononucleosis (IM). We previously reported that a partially purified preparation of this glycoprotein, when attached to latex beads, agglutinated in the presence of IM heterophile antibody...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.18.3.495-499.1983
更新日期：1983-09-01 00:00:00
abstract：:During an 8.5-year period (1974 to 1982), 56 Yersinia enterocolitica isolates from patients residing mainly in the New York City area were studied. Evaluation of these isolates revealed a marked increase in bacteremic episodes caused by strains of serogroups of O:3 and O:5,27 and the overall emergence of serogroup O:3...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.17.1.63-67.1983
更新日期：1983-01-01 00:00:00
abstract：:The performance of the Amplified Mycobacterium Tuberculosis Direct (AMTD) test (Gen-Probe Inc., San Diego, Calif.) was assessed in a large tertiary care mycobacteriology laboratory. Both acid-fast smear-positive and smear-negative respiratory and nonrespiratory clinical specimens were analyzed. From February 1998 to 4...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.40.5.1723-1727.2002
更新日期：2002-05-01 00:00:00
abstract：:The noninvasive diagnosis of amebic liver abscess is challenging, as most patients at the time of diagnosis do not have a concurrent intestinal infection with Entamoeba histolytica. Fecal testing for E. histolytica parasite antigen or DNA is negative in most patients. A real-time PCR assay was evaluated for detection ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.00152-10
更新日期：2010-08-01 00:00:00
abstract：:The human metapneumovirus (hMPV) was recently identified and linked to acute respiratory tract infections (ARTI). To assess the clinical importance of this virus in infants and children, we developed a rapid and efficient reverse transcription-PCR-based screening method for a large volume of samples and tested retrosp...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.43.11.5520-5525.2005
更新日期：2005-11-01 00:00:00
abstract：:Pneumocystis jirovecii pneumonia (PcP) is a major cause of respiratory illness in patients with AIDS. The identification of multiple single-nucleotide polymorphisms (SNPs) at three distinct P. jirovecii loci encoding dihydrofolate reductase (DHFR), mitochondrial large-subunit rRNA (mtLSU rRNA), and superoxide dismutas...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.02303-10
更新日期：2011-05-01 00:00:00
abstract：:Twelve bacterial species including Streptococcus uberis, S. parauberis, S. agalactiae, S. dysgalactiae, S. bovis, S. mitis, S. salivarius, S. saccharolyticus, Enterococcus faecium, E. faecalis, E. avium, and Aerococcus viridans were examined for their 16S ribosomal DNA fingerprint patterns. Oligonucleotide primers com...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.30.9.2235-2240.1992
更新日期：1992-09-01 00:00:00
abstract：:A typing enzyme immunoassay (TYPE-EIA) was used to determine the antigenic types of 64 astrovirus-positive specimens from nine collections from seven countries. Six of the seven known astrovirus types were detected in the collections, with HAstV-1 predominating in all collections for one from the United Kingdom. Selec...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.33.4.797-801.1995
更新日期：1995-04-01 00:00:00
abstract：:To study the population structure of Enterococcus faecalis from Polish hospitals, 291 isolates were typed by pulsed-field gel electrophoresis and a novel multilocus sequence typing scheme (P. Ruiz-Garbajosa et al., J. Clin. Microbiol. 44:2220-2228, 2006). The isolates originated from geographically widespread medical ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.01704-06
更新日期：2007-01-01 00:00:00
abstract：:Detection of human cytomegalovirus (CMV) DNA in clinical specimens is considered a cornerstone in the diagnosis of CMV disease. The aim of this study was to evaluate a newly designed LightCycler-based quantitative CMV PCR. Specimens of human origin (n = 200) were tested using the LightCycler PCR, the quantitative COBA...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/JCM.38.11.4006-4009.2000
更新日期：2000-11-01 00:00:00
abstract：:Herpes infections are among the most common sexually transmitted diseases and are the most common cause of genital ulcer disease in the United States. This study addresses the changing distribution of herpes simplex virus type 1 (HSV-1) and HSV-2 in patients presenting for evaluation of herpetic infections. Viral cult...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.39.9.3321-3325.2001
更新日期：2001-09-01 00:00:00
abstract：:A large pool of refined endotoxin was prepared from Escherichia coli O113 by extraction with hot acqueous phenol. It was characterized chemically and biologically and will be available for a reference standard designated as reference endotoxin EC. ...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:Herpes simplex virus (HSV) infections are very common in the general population and among immunocompromised patients. Acyclovir (ACV) is an effective treatment which is widely used. We deemed it essential to conduct a wide and coordinated survey of the emergence of ACV-resistant HSV strains. We have formed a network o...
journal_title：Journal of clinical microbiology
pub_type： 杂志文章
doi：10.1128/jcm.42.1.242-249.2004
更新日期：2004-01-01 00:00:00
