Method for phenotypic detection of extended-spectrum beta-lactamases in enterobacter species in the routine clinical setting.

abstract：:This is the first reported case of native valve endocarditis caused by Engyodontium album. This fungus, rarely seen as a human pathogen, is separated from Tritirachium species by its lack of pigmentation and from Beauveria species by the presence of conidiogenous cells in whorls. ...

journal_title：Journal of clinical microbiology

authors： Augustinsky J,Kammeyer P,Husain A,deHoog GS,Libertin CR

更新日期：1990-06-01 00:00:00

abstract：:Haemophilus ducreyi ATCC 33922, a virulent, well-characterized strain, was used to construct a genomic library in a bacteriophage expression vector. Three DNA fragments were selected for use as probes on the basis of their ability to encode H. ducreyi-specific proteins, as demonstrated by reactivity with rabbit polycl...

journal_title：Journal of clinical microbiology

authors： Parsons LM,Shayegani M,Waring AL,Bopp LH

更新日期：1989-07-01 00:00:00

abstract：:A method of inactivating mycoplasma contaminants in sera by using binary ethyleneimine was tried. When used at the concentration recommended for inactivating viruses, 0.001 M, binary ethyleneimine inactivated only two of five mycoplasma species. Even at a concentration of 0.01 M, the compound did not consistently inac...

journal_title：Journal of clinical microbiology

authors： Christianson GG,Pemberton JR,Koski TA

更新日期：1980-04-01 00:00:00

abstract：:Three immunoelectron microscopy (IEM) methods were employed to show laboratory-cultivated Francisella tularensis. By the IEM assays, F. tularensis was distinguished from four antigenically distinct gram-negative bacteria. IEM should be a valuable tool for confirming presumptive isolates of F. tularensis and may potent...

journal_title：Journal of clinical microbiology

authors： Geisbert TW,Jahrling PB,Ezzell JW Jr

更新日期：1993-07-01 00:00:00

abstract：:We describe a rare case of Francisella novicida bacteremia following a near-drowning event in seawater. We highlight the challenges associated with laboratory identification of F. novicida and differences in the epidemiology of F. novicida and Francisella tularensis infections. ...

journal_title：Journal of clinical microbiology

authors： Brett M,Doppalapudi A,Respicio-Kingry LB,Myers D,Husband B,Pollard K,Mead P,Petersen JM,Whitener CJ

更新日期：2012-08-01 00:00:00

abstract：:In this study, the currently known typing methods for Mycobacterium tuberculosis isolates were evaluated with regard to reproducibility, discrimination, and specificity. Therefore, 90 M. tuberculosis complex strains, originating from 38 countries, were tested in five restriction fragment length polymorphism (RFLP) typ...

journal_title：Journal of clinical microbiology

authors： Kremer K,van Soolingen D,Frothingham R,Haas WH,Hermans PW,Martín C,Palittapongarnpim P,Plikaytis BB,Riley LW,Yakrus MA,Musser JM,van Embden JD

更新日期：1999-08-01 00:00:00

abstract：:A reverse transcriptase PCR (RT-PCR) was used for rapid determination of the hemagglutinin (HA) cleavage site sequence, a marker for the virulence potential of avian influenza viruses. When applied to specimens from chickens experimentally infected with either a virulent or an avirulent virus, RT-PCR uniformly detecte...

journal_title：Journal of clinical microbiology

authors： Horimoto T,Kawaoka Y

更新日期：1995-03-01 00:00:00

abstract：:Serological interrelations among 46 strains of Haemophilus somnus of various geographical and pathological origins were studied by agglutination and agglutinin adsorption procedures. Sera prepared against nine representative American and Swiss isolates agglutinated all other strains to various titers. Adsorption of an...

journal_title：Journal of clinical microbiology

authors： Canto GJ,Biberstein EL

更新日期：1982-06-01 00:00:00

abstract：:Actinobacillus actinomycetemcomitans is an important pathogen in the etiology of severe periodontitis. For epidemiological studies on the prevalence of certain pathogenic clones and transmission of this bacterium, adequate typing methods are necessary. The purpose of this study was to compare six different typing meth...

journal_title：Journal of clinical microbiology

authors： van Steenbergen TJ,Bosch-Tijhof CJ,van Winkelhoff AJ,Gmür R,de Graaff J

更新日期：1994-11-01 00:00:00

abstract：:A new direct immunofluorescence monoclonal antibody (DFA) method (Genetic Systems, Inc., Seattle, Wash.) for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens was compared in a blinded study with an established Giemsa stain method. We evaluated 148 consecutive clinical speci...

journal_title：Journal of clinical microbiology

authors： Wolfson JS,Waldron MA,Sierra LS

更新日期：1990-09-01 00:00:00

abstract：:Despite attempts to control avian mycoplasmosis through management, vaccination, and surveillance, Mycoplasma gallisepticum continues to cause significant morbidity, mortality, and economic losses in poultry production. Live attenuated vaccines are commonly used in the poultry industry to control avian mycoplasmosis; ...

journal_title：Journal of clinical microbiology

authors： Ricketts C,Pickler L,Maurer J,Ayyampalayam S,García M,Ferguson-Noel NM

更新日期：2016-12-28 00:00:00

abstract：:Mycobacterium avium subsp. paratuberculosis is shed into the milk and feces of cows with advanced Johne's disease, allowing the transmission of M. avium subsp. paratuberculosis between animals. The objective of this study was to formulate an optimized protocol for the isolation of M. avium subsp. paratuberculosis in m...

journal_title：Journal of clinical microbiology

authors： Bradner L,Robbe-Austerman S,Beitz DC,Stabel JR

更新日期：2013-07-01 00:00:00

abstract：:Sapporo-like viruses (SLVs) are associated with acute gastroenteritis in humans. Due to a limited supply of available reagents for diagnosis, little is known about the incidence and pathogenicity of these viruses. We have developed a first-generation generic reverse transcriptase (RT) PCR assay based on a single prime...

journal_title：Journal of clinical microbiology

authors： Vinjé J,Deijl H,van der Heide R,Lewis D,Hedlund KO,Svensson L,Koopmans MP

更新日期：2000-02-01 00:00:00

abstract：:Porphyromonas gingivalis is associated with the development of periodontitis. Here we describe the development of a highly specific protease-based diagnostic method for the detection of P. gingivalis in gingival crevicular fluid. Screening of a proteolytic peptide substrate library, including fluorogenic dipeptides th...

journal_title：Journal of clinical microbiology

authors： Kaman WE,Galassi F,de Soet JJ,Bizzarro S,Loos BG,Veerman EC,van Belkum A,Hays JP,Bikker FJ

更新日期：2012-01-01 00:00:00

abstract：:The ability to rapidly recognize Ebola virus infections is critical to quickly limit further spread of the disease. A rapid, sensitive, and specific laboratory diagnostic test is needed to confirm outbreaks of Ebola virus infection and to distinguish it from other diseases that can cause similar clinical symptoms. A o...

journal_title：Journal of clinical microbiology

authors： Gibb TR,Norwood DA Jr,Woollen N,Henchal EA

更新日期：2001-11-01 00:00:00

abstract：:Resistance to linezolid is rare in clinical isolates of Enterococcus faecalis. A strain resistant to this antimicrobial but susceptible to vancomycin was found to cause central venous catheter colonization in a patient who never received linezolid. ...

journal_title：Journal of clinical microbiology

authors： Marra AR,Major Y,Edmond MB

更新日期：2006-05-01 00:00:00

abstract：:Fimbriae of Porphyromonas gingivalis are filamentous appendages on the cell surface and are thought to be one of the virulence factors. The fimA gene encoding the subunit protein of fimbriae, fimbrillin (FimA), was classified into four typeable variants (types I to IV). We previously examined the distribution of P. gi...

journal_title：Journal of clinical microbiology

authors： Nakagawa I,Amano A,Kimura RK,Nakamura T,Kawabata S,Hamada S

更新日期：2000-05-01 00:00:00

abstract：:Four methods for the detection of Trichomonas vaginalis in vaginal secretions from 88 symptomatic patients were compared: wet-mount examination, Kupferberg liquid medium, Hirsch charcoal agar, and the Papanicolaou smear. Hirsch diphasic slants and Kupferberg medium did not significantly differ in sensitivity from dire...

journal_title：Journal of clinical microbiology

authors： Thomason JL,Gelbart SM,Sobun JF,Schulien MB,Hamilton PR

更新日期：1988-09-01 00:00:00

abstract：:Multiple-locus variable-number tandem-repeat analysis (MLVA) was performed with 292 methicillin-resistant Staphylococcus aureus (MRSA) isolates previously characterized by pulsed-field gel electrophoresis, multilocus sequence typing, and staphylococcal cassette chromosome mec typing. Quantitative correspondence analys...

journal_title：Journal of clinical microbiology

authors： Rivero-Pérez B,Pérez-Roth E,Méndez-Alvarez S

更新日期：2010-08-01 00:00:00

abstract：:Characterization of 102 Haemophilus strains by the Minitek differentiation system (BBL Microbiology Systems, Cockeysville, Md.) was compared with that by the conventional method. The results obtained by the two methods were in good agreement (P less than 0.001; test of independence). Therefore, this system was found t...

journal_title：Journal of clinical microbiology

authors： Kawakami Y,Okimura Y,Kanai M

更新日期：1981-11-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome has been devastating the swine industry since the late 1980s. The disease has been controlled, to some extent, through the use of modified live-attenuated (MLV) vaccines once available. However, such a practice periodically resulted in isolation or detection of vaccine-lik...

journal_title：Journal of clinical microbiology

authors： Key KF,Guenette DK,Yoon KJ,Halbur PG,Toth TE,Meng XJ

更新日期：2003-06-01 00:00:00

abstract：:A monoclonal antibody (MAb), designated CHA 437, was developed against herpes simplex virus (HSV). This MAb (isotype, immunoglobulin G2b K) reacted with HSV type 1 and HSV type 2. It showed no cross-reactivity with varicella-zoster virus, cytomegalovirus, or Epstein-Barr virus. Direct detection of HSV antigen in clini...

journal_title：Journal of clinical microbiology

authors： Pouletty P,Chomel JJ,Thouvenot D,Catalan F,Rabillon V,Kadouche J

更新日期：1987-05-01 00:00:00

abstract：:A study of 180 healthy cats found that 15.6% were feline leukemia virus (FeLV) positive, 8.3% were feline immunodeficiency virus (FIV) positive, and 1.1% were FIV and FeLV positive, which corresponded to 30.4, 13.8, and 2.6, of 115 cats with FIV- and FeLV-related symptoms, respectively. Differences were seen in the se...

journal_title：Journal of clinical microbiology

authors： Arjona A,Escolar E,Soto I,Barquero N,Martin D,Gomez-Lucia E

更新日期：2000-09-01 00:00:00

abstract：:Antiserum specific for Salmonella O3 antigen was raised by immunization of rabbits with an artificial glycoconjugate consisting of the synthetic trisaccharide beta-D- Manp (1----4)-alpha-L- Rhap (1----3)-alpha-D-Galp covalently linked to bovine serum albumin (beta- MRG -BSA). Enzyme immunoassays showed that only lipop...

journal_title：Journal of clinical microbiology

authors： Ekwall E,Norberg T,Svenson SB,Lindberg AA

更新日期：1984-05-01 00:00:00

abstract：:The p1 genes of 60 Mycoplasma pneumoniae clinical isolates were sequenced and compared to previously reported p1 gene sequences. An AGT trinucleotide variable-number tandem repeat was identified that ranged in copy number from 5 to 14 among the isolates. In addition, a novel p1 gene variant named 2c was identified in ...

journal_title：Journal of clinical microbiology

authors： Zhao F,Cao B,Li J,Song S,Tao X,Yin Y,He L,Zhang J

更新日期：2011-08-01 00:00:00

abstract：:The protease phenotypes expressed by isolates of Pseudomonas aeruginosa from cystic fibrosis (CF) patients were evaluated. The majority of isolates tested produced elastase (65%) or alkaline protease (64%) or both. The mucoid phenotype expressed by many CF isolates of P. aeruginosa did not absolutely restrict the expr...

journal_title：Journal of clinical microbiology

authors： Jagger KS,Bahner DR,Warren RL

更新日期：1983-01-01 00:00:00

abstract：:Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. We recently reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable for serodiagnosis of ...

journal_title：Journal of clinical microbiology

authors： McBride JW,Corstvet RE,Breitschwerdt EB,Walker DH

更新日期：2001-01-01 00:00:00

abstract：:Diagnosis of Bordetella pertussis infection has been difficult due to the low sensitivity of culture. PCR tests have been shown to be more sensitive than culture, but the reported sensitivity of PCR is variable. We evaluated PCR product detection by using either agarose gel electrophoresis (PCR-gel) or dot blot hybrid...

journal_title：Journal of clinical microbiology

authors： Lingappa JR,Lawrence W,West-Keefe S,Gautom R,Cookson BT

更新日期：2002-08-01 00:00:00

abstract：:The DNA hybridization assay for genes encoding for Escherichia coli enterotoxins was used to examine water specimens in Thailand. In a reconstruction experiment, the DNA hybridization assay was 10(4) times more sensitive than testing random E. coli in the Y-1 adrenal and suckling mouse assays in identifying enterotoxi...

journal_title：Journal of clinical microbiology

authors： Echeverria P,Seriwatana J,Chityothin O,Chaicumpa W,Tirapat C

更新日期：1982-12-01 00:00:00

abstract：:The recently described genus Pandoraea contains five named species (Pandoraea apista, Pandoraea pulmonicola, Pandoraea pnomenusa, Pandoraea sputorum, and Pandoraea norimbergensis) and four unnamed genomospecies. Pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (CF) patients. Acc...

journal_title：Journal of clinical microbiology

authors： Coenye T,Liu L,Vandamme P,LiPuma JJ

更新日期：2001-12-01 00:00:00