Development of a heteroduplex mobility assay to identify field isolates of porcine reproductive and respiratory syndrome virus with nucleotide sequences closely related to those of modified live-attenuated vaccines.


:Porcine reproductive and respiratory syndrome has been devastating the swine industry since the late 1980s. The disease has been controlled, to some extent, through the use of modified live-attenuated (MLV) vaccines once available. However, such a practice periodically resulted in isolation or detection of vaccine-like viruses from pigs as determined by a partial genomic sequencing. In this study, we developed a heteroduplex mobility assay (HMA) for quickly identifying porcine reproductive and respiratory syndrome virus (PRRSV) isolates with significant nucleotide sequence identities (>/=98%) with the modified live-attenuated vaccines. The major envelope gene (ORF5) of 51 PRRSV field isolates recovered before and after the introduction of the vaccines was amplified, denatured, and reannealed with the HMA reference vaccine strains Ingelvac PRRS MLV and Ingelvac PRRS ATP, respectively. Nine of the 51 field isolates and the VR2332 parent virus of Ingelvac PRRS MLV, which were all highly related to Ingelvac PRRS MLV with


J Clin Microbiol


Key KF,Guenette DK,Yoon KJ,Halbur PG,Toth TE,Meng XJ




Has Abstract


2003-06-01 00:00:00












  • Failure of cyclophosphamide to significantly enhance isolation of Rickettsia tsutsugamushi from wild Philippine rats.

    abstract::Treatment of wild rats from a known scrub typhus focus in the Philippines with cyclophosphamide did not significantly enhance the isolation rate of Rickettsia tsutsugamushi using mouse inoculation techniques. Similarly, cyclophosphamide treatment of mice inoculated with organs of wild rats did not increase the recover...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: van Peenen PF,Ho CM,See R

    更新日期:1977-01-01 00:00:00

  • Effects of volume and site of blood draw on blood culture results.

    abstract::Blood culture contamination greatly affects clinical decisions. Hence, it is of interest to assess the influence of factors such as the volume of blood drawn and the site of blood draw on the rates of blood culture contamination. In a retrospective study, blood cultures from infants and children up to 18 years of age ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gonsalves WI,Cornish N,Moore M,Chen A,Varman M

    更新日期:2009-11-01 00:00:00

  • Direct comparison of the BACTEC 9240 and BacT/ALERT 3D automated blood culture systems for candida growth detection.

    abstract::A direct comparison of two automated blood culture systems was conducted to compare their ability to detect Candida growth. The systems evaluated were the BACTEC 9240 (Bactec) and BacT/ALERT 3D (BacT). The aerobic, anaerobic, and mycology media for each system were evaluated: Bactec Plus Aerobic/F, Plus Anaerobic/F, a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Horvath LL,George BJ,Murray CK,Harrison LS,Hospenthal DR

    更新日期:2004-01-01 00:00:00

  • Escherichia fergusonii bacteremia in a diabetic patient with pancreatic cancer.

    abstract::Although Escherichia fergusonii has been identified for decades, it has rarely been recovered from clinical specimens and its clinical significance remains unclear. We describe a case of E. fergusonii bacteremia in a diabetic patient with pancreatic cancer. The isolate was confirmed by three commercial identification ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lai CC,Cheng A,Huang YT,Chung KP,Lee MR,Liao CH,Hsueh PR

    更新日期:2011-11-01 00:00:00

  • In vitro activities of ampicillin-sulbactam and amoxicillin-clavulanic acid against Acinetobacter baumannii.

    abstract::In vitro susceptibility patterns of newer beta-lactamase-inhibiting antibiotics ampicillin-sulbactam (A/S) and amoxicillin-clavulanic acid (A/C) for 100 consecutive isolates of Acinetobacter baumannii obtained from various clinical samples were studied. The A/C MIC for 86% of the strains was more than 16/8 microgram/m...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Pandey A,Kapil A,Sood S,Goel V,Das B,Seth P

    更新日期:1998-11-01 00:00:00

  • New latex reagent using monoclonal antibodies to capsular polysaccharide for reliable identification of both oxacillin-susceptible and oxacillin-resistant Staphylococcus aureus.

    abstract::A new latex agglutination test (Pastorex Staph-Plus, Sanofi Diagnostics Pasteur), consisting of a mixture of latex particles coated with fibrinogen and immunoglobulin G for the detection of clumping factor and protein A and latex particles sensitized with monoclonal antibodies directed to Staphylococcus aureus serotyp...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Fournier JM,Bouvet A,Mathieu D,Nato F,Boutonnier A,Gerbal R,Brunengo P,Saulnier C,Sagot N,Slizewicz B

    更新日期:1993-05-01 00:00:00

  • Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis.

    abstract::A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuab...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Prod'hom G,Guilhot C,Gutierrez MC,Varnerot A,Gicquel B,Vincent V

    更新日期:1997-12-01 00:00:00

  • Development of a multitarget real-time TaqMan PCR assay for enhanced detection of Francisella tularensis in complex specimens.

    abstract::Tularemia is the zoonotic disease caused by the gram-negative coccobacillus Francisella tularensis. Its wide distribution in the environment poses a challenge for understanding the transmission, ecology, and epidemiology of the disease. F. tularensis is also considered a potential biological weapon due to its extreme ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Versage JL,Severin DD,Chu MC,Petersen JM

    更新日期:2003-12-01 00:00:00

  • Temporal changes in outer surface proteins A and C of the lyme disease-associated spirochete, Borrelia burgdorferi, during the chain of infection in ticks and mice.

    abstract::The Lyme disease-associated spirochete, Borrelia burgdorferi, is maintained in enzootic cycles involving Ixodes ticks and small mammals. Previous studies demonstrated that B. burgdorferi expresses outer surface protein A (OspA) but not OspC when residing in the midgut of unfed ticks. However, after ticks feed on blood...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Schwan TG,Piesman J

    更新日期:2000-01-01 00:00:00

  • Comparison of pooled formalin-preserved fecal specimens with three individual samples for detection of intestinal parasites.

    abstract::Triplicate preserved fecal samples from 265 patients were pooled into single samples, and the recovery rate of intestinal parasites from the pooled samples was compared with that from the individual samples. Parasites were detected in 109 patients when results from the individual samples were used compared with 108 pa...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Aldeen WE,Shisenant J,Hale D,Matsen J,Carroll K

    更新日期:1993-01-01 00:00:00

  • Molecular typing of the etiologic agent of human granulocytic ehrlichiosis.

    abstract::The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with HindIII, EcoRV, and AspI to generate restriction fragmen...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Carter SE,Ravyn MD,Xu Y,Johnson RC

    更新日期:2001-09-01 00:00:00

  • Evaluation of a novel rapid one-step immunochromatographic assay for detection of monoclonal Helicobacter pylori antigen in stool samples from children.

    abstract::A new rapid one-step immunochromatographic test using monoclonal antibodies for detection of Helicobacter pylori antigen in stool in children was evaluated on coded stool samples from 159 children (mean age, 9.7 +/- 5.0 years; 118 from Munich, 41 from Vienna): 86 children were H. pylori infected defined by positive cu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Antos D,Crone J,Konstantopoulos N,Koletzko S

    更新日期:2005-06-01 00:00:00

  • Neuraminidase activity is not the cause of influenza virus-induced neutrophil dysfunction.

    abstract::Influenza viruses have been shown to decrease the ability of polymorphonuclear leukocytes (PMN) to respond to a variety of stimuli. This study was done to determine if viral neuraminidase was responsible for decreased PMN function. Treatment of human PMN with purified neuraminidases from influenza virus, Vibrio choler...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Abramson JS,Wiegand GL,Lyles DS

    更新日期:1985-07-01 00:00:00

  • Nested PCR for specific diagnosis of Taenia solium taeniasis.

    abstract::Taeniasis due to Taenia solium is a disease with important public health consequences, since the larval stage is not exclusive to the animal intermediate, the pig, but also infects humans, causing neurocysticercosis. Early diagnosis and treatment of T. solium tapeworm carriers is important to prevent human cysticercos...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mayta H,Gilman RH,Prendergast E,Castillo JP,Tinoco YO,Garcia HH,Gonzalez AE,Sterling CR,Cysticercosis Working Group in Peru.

    更新日期:2008-01-01 00:00:00

  • Salivary immunoglobulin G assay to diagnose Helicobacter pylori infection in children.

    abstract::An in-house enzyme-linked immunosorbent assay (ELISA) for measurement of Helicobacter pylori-specific immunoglobulin G (IgG) and IgA in saliva was evaluated by comparison with histopathologic (Giemsa staining) and biochemical (urease quick test) examination of gastric biopsy specimens obtained from 112 children referr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Luzza F,Oderda G,Maletta M,Imeneo M,Mesuraca L,Chioboli E,Lerro P,Guandalini S,Pallone F

    更新日期:1997-12-01 00:00:00

  • Distinct diversity of vacA, cagA, and cagE genes of Helicobacter pylori associated with peptic ulcer in Japan.

    abstract::Colonization of the stomach mucosa by Helicobacter pylori is a major cause of acute and chronic gastric pathologies in humans. Several H. pylori virulence genes that may play a role in its pathogenicity have been identified. The most important determinants are vacA and cagA in the cag pathogenicity island (cagPAI) gen...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Yamazaki S,Yamakawa A,Okuda T,Ohtani M,Suto H,Ito Y,Yamazaki Y,Keida Y,Higashi H,Hatakeyama M,Azuma T

    更新日期:2005-08-01 00:00:00

  • Antimicrobial susceptibility of Helicobacter pylori determined by the E test using tetrazolium egg yolk agar.

    abstract::Metronidazole and tetracycline E tests were compared to an agar dilution method for the antimicrobial susceptibility testing of Helicobacter pylori. Sixteen strains were tested by using tetrazolium egg yolk (TEY) agar. The characteristic E test inhibition ellipse was clearer on TEY agar than on standard blood agar and...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Valdez Y,Velapatiño B,Gilman RH,Gutierrez V,León C

    更新日期:1998-09-01 00:00:00

  • Characterization of Neisseria elongata subsp. glycolytica isolates obtained from human wound specimens and blood cultures.

    abstract::Four slightly yellow-pigmented, alpha-hemolytic, gram-negative coccobacilli, three from wound specimens and one from multiple blood cultures of a patient with endocarditis, were identified as Neisseria elongata subsp. glycolytica on the basis of their overall biochemical and genetic similarities to this subspecies. Th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Andersen BM,Weyant RS,Steigerwalt AG,Moss CW,Hollis DG,Weaver RE,Ashford D,Brenner DJ

    更新日期:1995-01-01 00:00:00

  • Invasive strain of Escherichia coli belonging to O group 29.

    abstract::A strain of Escherichia coli isolated from a child with diarrhea was biochemically similar and antigenically identical (O and K antigens) to the standard strain of E. coli O group 29 and was positive in the Serény test for invasiveness, which suggests that it can cause a Shigella-like illness. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Toledo MR,Reis MH,Almeida RG,Trabulsi LR

    更新日期:1979-02-01 00:00:00

  • Detection of antibodies to legionnaires disease organism by microagglutination and micro-enzyme-linked immunosorbent assay tests.

    abstract::Microagglutination and micro-enzyme-linked immunosorbent assay (ELISA) tests with easily prepared, safe, heat-killed antigens for detecting antibodies to the legionnaires disease organism have been developed. A safranin-stained whole-cell antigen is used in the microagglutination test, and a simply prepared soluble an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Farshy CE,Klein GC,Feeley JC

    更新日期:1978-04-01 00:00:00

  • Defined medium simulating genital tract secretions for growth of vaginal microflora.

    abstract::A chemically defined medium that simulates female genital tract secretions was developed for the growth of the vaginal microflora. Qualitative and quantitative studies of the growth of predominant components of the vaginal microflora indicated that all vaginal isolates tested were able to grow in this defined medium. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Geshnizgani AM,Onderdonk AB

    更新日期:1992-05-01 00:00:00

  • Evaluation of an Automated Instrument for Inoculating and Spreading Samples onto Agar Plates.

    abstract::The findings from a preliminary assessment of a new instrument designed for the inoculation and spreading of specimens for microbiological analysis onto agar plates are described. The study found that the instrument was able to select full or biplates from a number of input cassettes, each containing different agar ty...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Glasson JH,Guthrie LH,Nielsen DJ,Bethell FA

    更新日期:2008-04-01 00:00:00

  • Comparison of MagPix Assays and Enzyme-Linked Immunosorbent Assay for Detection of Hemorrhagic Fever Viruses.

    abstract::Viral hemorrhagic fevers, because of their high mortality rates, the lack of medical countermeasures, and their potential use as instruments of bioterrorism, pose a significant threat to the developed and the developing areas of the world. The key to preventing the spread of these diseases is early and accurate detect...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Satterly NG,Voorhees MA,Ames AD,Schoepp RJ

    更新日期:2016-12-28 00:00:00

  • Candida guilliermondii and other species of candida misidentified as Candida famata: assessment by vitek 2, DNA sequencing analysis, and matrix-assisted laser desorption ionization-time of flight mass spectrometry in two global antifungal surveillance pro

    abstract::Candida famata (teleomorph Debaryomyces hansenii) has been described as a medically relevant yeast, and this species has been included in many commercial identification systems that are currently used in clinical laboratories. Among 53 strains collected during the SENTRY and ARTEMIS surveillance programs and previousl...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Castanheira M,Woosley LN,Diekema DJ,Jones RN,Pfaller MA

    更新日期:2013-01-01 00:00:00

  • Infection of Syrian hamsters with Lyme disease spirochetes.

    abstract::Syrian hamsters were shown to be susceptible to infection by the Lyme disease spirochete Borrelia burgdorferi. Although these spirochetes did not cause any outward signs of illness in these animals, they did cause a generalized infection. Spirochetemia was present during the first 6 days of infection. At 14 days posti...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Johnson RC,Marek N,Kodner C

    更新日期:1984-12-01 00:00:00

  • Genome sequencing of Mycobacterium abscessus isolates from patients in the united states and comparisons to globally diverse clinical strains.

    abstract::Nontuberculous mycobacterial infections caused by Mycobacterium abscessus are responsible for a range of disease manifestations from pulmonary to skin infections and are notoriously difficult to treat, due to innate resistance to many antibiotics. Previous population studies of clinical M. abscessus isolates utilized ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Davidson RM,Hasan NA,Reynolds PR,Totten S,Garcia B,Levin A,Ramamoorthy P,Heifets L,Daley CL,Strong M

    更新日期:2014-10-01 00:00:00

  • Rapid identification of microorganisms from sterile body fluids by use of FilmArray.

    abstract::We evaluated the clinical performance of the FilmArray blood culture identification (BCID) panel in the identification of microorganisms from positive blood culture bottles inoculated with sterile body fluids. All organisms included in the FA BCID panel were accurately identified in 84/84 (100%) and 18/24 (75%) sample...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Altun O,Almuhayawi M,Ullberg M,Özenci V

    更新日期:2015-02-01 00:00:00

  • Marked differences in quantity of infectious human immunodeficiency virus type 1 detected in persons with controlled plasma viremia by a simple enhanced culture method.

    abstract::Culture of autologous CD4 lymphocytes from peripheral blood mononuclear cells compared favorably with two other methods for the measurement of cell-associated human immunodeficiency virus type 1 (HIV-1). For subjects with undetectable HIV-1 RNA levels in plasma, there was a 10,000-fold range of cell-associated virus d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Tremblay CL,Giguel F,Merrill DP,Wong JT,Rosenberg E,Kalams S,Walker BD,D'Aquila RT,Hirsch MS

    更新日期:2000-11-01 00:00:00

  • Detection and Prevalence of Penicillin-Susceptible Staphylococcus aureus in the United States in 2013.

    abstract::Using blaZ PCR as the "gold standard," the sensitivities of CLSI penicillin zone edge and nitrocefin-based tests for β-lactamase production in Staphylococcus aureus were 64.5% and 35.5%, respectively, with specificity of 99.8% for both methods. In 2013, 13.5% of 3,083 S. aureus isolates from 31 U.S. centers were penic...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Richter SS,Doern GV,Heilmann KP,Miner S,Tendolkar S,Riahi F,Diekema DJ

    更新日期:2016-03-01 00:00:00

  • Susceptibility of Neisseria meningitidis to 16 antimicrobial agents and characterization of resistance mechanisms affecting some agents.

    abstract::Neisseria meningitidis represents a pathogen of great public health importance in both developed and developing countries. Resistance to some antimicrobial agents used either for therapy of invasive infections or for prophylaxis of case contacts has long been recognized, although specific guidelines for susceptibility...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Jorgensen JH,Crawford SA,Fiebelkorn KR

    更新日期:2005-07-01 00:00:00