Development of a heteroduplex mobility assay to identify field isolates of porcine reproductive and respiratory syndrome virus with nucleotide sequences closely related to those of modified live-attenuated vaccines.

abstract：:It is unclear whether patients colonized with methicillin-resistant Staphylococcus aureus (MRSA) continue to harbor the same genotype during hospital readmissions. We characterized 140 MRSA strains isolated from 33 persistent MRSA carriers with hospital readmissions. Nearly half of the patients continued to harbor the...

journal_title：Journal of clinical microbiology

authors： Eghbaria S,Schnitser K,Danial-Farran N,Keness Y,Bisharat N

更新日期：2014-04-01 00:00:00

abstract：:A group of 76 consecutive human immunodeficiency virus (HIV)-positive patients with fever of unknown origin (n = 52) or fever associated with pulmonary diseases was evaluated in order to assess the usefulness of PCR with peripheral blood in the diagnosis and follow-up of visceral leishmaniasis. We identified 10 cases ...

journal_title：Journal of clinical microbiology

authors： Pizzuto M,Piazza M,Senese D,Scalamogna C,Calattini S,Corsico L,Persico T,Adriani B,Magni C,Guaraldi G,Gaiera G,Ludovisi A,Gramiccia M,Galli M,Moroni M,Corbellino M,Antinori S

更新日期：2001-01-01 00:00:00

abstract：:Chlamydia pneumoniae has proved to be difficult to isolate and propagate in cell culture. We compared the growth of three strains of C. pneumoniae, TW-183 and two clinical isolates from Brooklyn, N.Y., in five cell lines, including HeLa 229, McCoy, HL, HEp-2, and HTED, an immortalized human tracheal cell line. HEp-2 w...

journal_title：Journal of clinical microbiology

authors： Roblin PM,Dumornay W,Hammerschlag MR

更新日期：1992-08-01 00:00:00

abstract：:The efficacy of antiviral treatment for chronic hepatitis C virus (HCV) infection is determined by measuring HCV RNA at specific time points throughout therapy using highly sensitive and accurate HCV RNA assays. This study compared the performances of two recently developed real-time PCR HCV RNA assays, cobas HCV for ...

journal_title：Journal of clinical microbiology

authors： Vermehren J,Stelzl E,Maasoumy B,Michel-Treil V,Berkowski C,Marins EG,Paxinos EE,Marino E,Wedemeyer H,Sarrazin C,Kessler HH

更新日期：2017-04-01 00:00:00

abstract：:The etiological agents of diarrhea in Vientiane, Lao People's Democratic Republic (Lao PDR), were studied in the period from October 1996 to August 1997. A total of 880 patients with diarrhea visiting medical facilities were examined for Shigella, Salmonella, diarrheagenic Escherichia coli, vibrio, Aeromonas, Campylob...

journal_title：Journal of clinical microbiology

authors： Yamashiro T,Nakasone N,Higa N,Iwanaga M,Insisiengmay S,Phounane T,Munnalath K,Sithivong N,Sisavath L,Phanthauamath B,Chomlasak K,Sisulath P,Vongsanith P

更新日期：1998-08-01 00:00:00

abstract：:We determined the entire nucleotide sequence of phiSa2958-carrying Panton-Valentine leukocidin (PVL) gene, which was lysogenized in a sequence type 5 staphylococcal cassette chromosome mec (SCCmec) type II strain of methicillin-resistant Staphylococcus aureus (MRSA). Based on the nucleotide sequences of PVL phages, we...

journal_title：Journal of clinical microbiology

authors： Ma XX,Ito T,Kondo Y,Cho M,Yoshizawa Y,Kaneko J,Katai A,Higashiide M,Li S,Hiramatsu K

更新日期：2008-10-01 00:00:00

abstract：:A group of 300 clinically derived isolates of coagulase-negative staphylococci were tested in parallel with the API STAPH-IDENT system (Analytab Products) and 14 conventional biochemical tests contained in Kloos and Schleifer's simplified scheme for identification of human Staphylococcus species. STAPH-IDENT is a mini...

journal_title：Journal of clinical microbiology

authors： Almeida RJ,Jorgensen JH

更新日期：1983-08-01 00:00:00

abstract：:Between January 1988 and December 1989 Aeromonas species were isolated from 45 (1.8%) of 2,480 patients with acute gastroenteritis. No other bacterial enteric pathogens were found in any of these 45 patients. Of the 45 Aeromonas isolates, 35 strains (77.8%) were Aeromonas hydrophila, 7 (15.5%) were Aeromonas sobria, a...

journal_title：Journal of clinical microbiology

authors： Deodhar LP,Saraswathi K,Varudkar A

更新日期：1991-05-01 00:00:00

abstract：:During February and March 1984, 207 fecal samples from infants and children with gastroenteritis were tested for rotavirus with four techniques: two enzyme immunoassays (Rotazyme; Abbott Laboratories, North Chicago, Ill., and Enzygnost-Rotavirus; Calbiochem-Behring, La Jolla, Calif.) and two latex agglutination tests ...

journal_title：Journal of clinical microbiology

authors： Sambourg M,Goudeau A,Courant C,Pinon G,Denis F

更新日期：1985-04-01 00:00:00

abstract：:The polymerase chain reaction was used to detect human herpesvirus 6 (HHV-6) DNA in peripheral blood mononuclear cells from patients with exanthem subitum. Amplified products were detected by agarose gel electrophoresis and dot blot hybridization with a cloned DNA probe. No cross-hybridization with DNAs of five other ...

journal_title：Journal of clinical microbiology

authors： Kondo K,Hayakawa Y,Mori H,Sato S,Kondo T,Takahashi K,Minamishima Y,Takahashi M,Yamanishi K

更新日期：1990-05-01 00:00:00

abstract：:Tropheryma whipplei, the agent of Whipple's disease, is a gram-positive rod-shaped bacterium that belongs to the group of actinobacteria. In order to produce monoclonal antibodies (MAbs) against this bacterium, we inoculated mice with two different strains, Slow2 and Endo5. We produced 13 and 10 MAbs against Slow2 and...

journal_title：Journal of clinical microbiology

authors： Yu Y,Kowalczewska M,Decloquement P,Nappez C,La Scola B

更新日期：2006-11-01 00:00:00

abstract：:We present the first case of severe pneumonia possibly caused by Leptotrichia species with oral bacteria. This was found in a healthy but elderly subject whose bronchoalveolar lavage fluid was analyzed by 16S rRNA gene sequencing analysis. The combination of this method and microscopic observation provided useful info...

journal_title：Journal of clinical microbiology

authors： Kawanami T,Fukuda K,Yatera K,Kido T,Yoshii C,Taniguchi H,Kido M

更新日期：2009-02-01 00:00:00

abstract：:Fluorescence of sensitized bacterial suspensions, reactive with either fluorescein-labeled or rhodamine-labeled antiglobulins, could be quantitatively distinguished in dual-labeled preparations by fluorescence immunoassay. ...

journal_title：Journal of clinical microbiology

authors： Gillis TP,Thompson JJ

更新日期：1978-09-01 00:00:00

abstract：:Four Mycoplasma arthritidis strains, two virulent (158p10p9 and 14124p10) and two avirulent (PG6 and H606), were examined for differences in their antigenic compositions. Rabbit antisera prepared against each strain were compared for their reactivities against both homologous and heterologous strains by enzyme-linked ...

journal_title：Journal of clinical microbiology

authors： Washburn LR,Hirsch S

更新日期：1990-09-01 00:00:00

abstract：:In a stress test, the recently introduced RapID CB Plus system (Remel Inc. [formerly Innovative Diagnostic Systems], Norcross, Ga.) was challenged with a diverse set of gram-positive rods comprising 345 strains of coryneform bacteria and 33 strains of Listeria spp. representing a total of 49 different taxa. Overall, w...

journal_title：Journal of clinical microbiology

authors： Funke G,Peters K,Aravena-Roman M

更新日期：1998-09-01 00:00:00

abstract：:The clinical value of immunoenzymatic (enzyme-linked immunosorbent assay) detection of anti-Toxoplasma immunoglobulin E (IgE) was assessed by studying 2,036 sera from 792 subjects, comprising seronegative controls and subjects with acute, active, reactivated, or congenital toxoplasmosis. Included were nonimmunized adu...

journal_title：Journal of clinical microbiology

authors： Foudrinier F,Villena I,Jaussaud R,Aubert D,Chemla C,Martinot F,Pinon JM

更新日期：2003-04-01 00:00:00

abstract：:A European multicenter study was performed to evaluate the performance of a new method, based on the transcription-reverse transcription concerted reaction (TRC-2), which enabled one-step amplification and real-time detection of the Mycobacterium tuberculosis 16S rRNA target directly in clinical specimens. A total of ...

journal_title：Journal of clinical microbiology

authors： Drouillon V,Delogu G,Dettori G,Lagrange PH,Benecchi M,Houriez F,Baroli K,Ardito F,Torelli R,Chezzi C,Fadda G,Herrmann JL

更新日期：2009-11-01 00:00:00

abstract：:The recently described genus Pandoraea contains five named species (Pandoraea apista, Pandoraea pulmonicola, Pandoraea pnomenusa, Pandoraea sputorum, and Pandoraea norimbergensis) and four unnamed genomospecies. Pandoraea spp. have mainly been recovered from the respiratory tracts of cystic fibrosis (CF) patients. Acc...

journal_title：Journal of clinical microbiology

authors： Coenye T,Liu L,Vandamme P,LiPuma JJ

更新日期：2001-12-01 00:00:00

abstract：:Rapid and accurate identification of mycobacterial species is essential for patient management. We describe the use of the Invader assay in conjunction with the BACTEC MGIT 960 system that together provide an efficient procedure for clinical use. This assay discriminates single-base differences (e.g., genotyping singl...

journal_title：Journal of clinical microbiology

authors： Ichimura S,Nagano M,Ito N,Shimojima M,Egashira T,Miyamoto C,Ohkusu K,Ezaki T

更新日期：2007-10-01 00:00:00

abstract：:A reverse transcriptase PCR (RT-PCR) was used for rapid determination of the hemagglutinin (HA) cleavage site sequence, a marker for the virulence potential of avian influenza viruses. When applied to specimens from chickens experimentally infected with either a virulent or an avirulent virus, RT-PCR uniformly detecte...

journal_title：Journal of clinical microbiology

authors： Horimoto T,Kawaoka Y

更新日期：1995-03-01 00:00:00

abstract：:Antisera prepared against the eight recognized serotypes of Ureaplasma urealyticum were tested against the homologous and heterologous antigens by a modified metabolic inhibition test that used bromothymol blue broth in microtiter plates. The method gives clear endpoint determinations which are usually maintained on c...

journal_title：Journal of clinical microbiology

authors： Robertson JA,Stemke GW

更新日期：1979-06-01 00:00:00

abstract：:Rapid diagnostic tests (RDTs) based on the detection of specific antibodies in serum are commonly used for the diagnosis of visceral leishmaniasis (VL). Several commercial kits are available, and some of them allow the use of whole-blood samples instead of serum. An RDT is much more user-friendly for blood samples tha...

journal_title：Journal of clinical microbiology

authors： Kumar D,Khanal B,Tiwary P,Mudavath SL,Tiwary NK,Singh R,Koirala K,Boelaert M,Rijal S,Sundar S

更新日期：2013-12-01 00:00:00

abstract：:We describe the serum immunoglobulin A (IgA) antibody response to Norwalk virus infection in human volunteers and compare it with previously described IgM and total antibody responses. Whereas specific IgA and IgM peak within 2 weeks after onset of symptoms, titers of total blocking antibody continue to rise, implying...

journal_title：Journal of clinical microbiology

authors： Erdman DD,Gary GW,Anderson LJ

更新日期：1989-06-01 00:00:00

abstract：:The rapid identification of blood culture isolates and antimicrobial susceptibility test (AST) results play critical roles for the optimal treatment of patients with bloodstream infections. Whereas others have looked at the time to detection in automated culture systems, we examined the overall time from specimen coll...

journal_title：Journal of clinical microbiology

authors： Tabak YP,Vankeepuram L,Ye G,Jeffers K,Gupta V,Murray PR

更新日期：2018-11-27 00:00:00

abstract：:Droplet digital PCR (ddPCR) is an emulsion PCR process that performs absolute quantitation of nucleic acids. We developed a ddPCR assay for Chlamydia trachomatis infections and found it to be accurate and precise. Using PCR mixtures containing plasmids engineered to include the PCR target sequences, we were able to qu...

journal_title：Journal of clinical microbiology

authors： Roberts CH,Last A,Molina-Gonzalez S,Cassama E,Butcher R,Nabicassa M,McCarthy E,Burr SE,Mabey DC,Bailey RL,Holland MJ

更新日期：2013-07-01 00:00:00

abstract：:We report the development of a real-time PCR assay for the quantitative detection of Neospora caninum in infected host tissues. The assay uses the double-stranded DNA-binding dye SYBR Green I to continuously monitor product formation. Oligonucleotide primers were designed to amplify a 76-bp DNA fragment corresponding ...

journal_title：Journal of clinical microbiology

authors： Collantes-Fernández E,Zaballos A,Alvarez-García G,Ortega-Mora LM

更新日期：2002-04-01 00:00:00

abstract：:A new DNA probe assay (INNO LiPA Mycobacteria; Innogenetics, Ghent, Belgium) for the simultaneous identification, by means of reverse hybridization and line-probe technology, of Mycobacterium tuberculosis complex, Mycobacterium kansasii, Mycobacterium xenopi, Mycobacterium gordonae, the species of the Mycobacterium av...

journal_title：Journal of clinical microbiology

authors： Tortoli E,Nanetti A,Piersimoni C,Cichero P,Farina C,Mucignat G,Scarparo C,Bartolini L,Valentini R,Nista D,Gesu G,Tosi CP,Crovatto M,Brusarosco G

更新日期：2001-03-01 00:00:00

abstract：:A recently described method for detecting rubella-specific immunoglobulin M (IgM) antibody based on absorption of IgG by Staphylococcus aureus strain Cowan I has been applied to 198 sera collected during a recent rubella epidemic in Israel. Modification of the original method introduced for the present study includes ...

journal_title：Journal of clinical microbiology

authors： Handsher R,Fogel A

更新日期：1977-06-01 00:00:00

abstract：:Vaginal swab specimens may be preferable to cervical swab or urine specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae because of the ease of specimen collection and transport. The purpose of this study was to evaluate whether vaginal swab specimens are equivalent to cervical swab specimens ...

journal_title：Journal of clinical microbiology

authors： Cosentino LA,Landers DV,Hillier SL

更新日期：2003-08-01 00:00:00

abstract：:Achromobacter species are increasingly being detected in cystic fibrosis (CF) patients, with an unclear epidemiology and impact. We studied a cohort of patients attending a Canadian adult CF clinic who had positive sputum cultures for Achromobacter species in the period from 1984 to 2013. Infection was categorized as ...

journal_title：Journal of clinical microbiology

authors： Edwards BD,Greysson-Wong J,Somayaji R,Waddell B,Whelan FJ,Storey DG,Rabin HR,Surette MG,Parkins MD

更新日期：2017-07-01 00:00:00