Multicenter evaluation of a transcription-reverse transcription concerted assay for rapid detection of mycobacterium tuberculosis complex in clinical specimens.

abstract：:Broth microdilution and disk diffusion susceptibility testing were performed on 73 strains of Pasteurella species isolated from human infections and on five American Type Culture Collection strains of Pasteurella species. Both methods appear reliable for testing susceptibilities of Pasteurella species. ...

journal_title：Journal of clinical microbiology

authors： Citron DM,Warren YA,Fernandez HT,Goldstein MA,Tyrrell KL,Goldstein EJ

更新日期：2005-05-01 00:00:00

abstract：:The relationship of coagulase-negative staphylococcal slime production and adherence with the development and outcome of bloodstream infections in two Finnish hospitals was evaluated. Analysis of 64 strains from 62 adult septicemias disclosed 34 (53%) adherent slime producers. In comparison, only 142 (29%) of 489 sing...

journal_title：Journal of clinical microbiology

authors： Kotilainen P

更新日期：1990-12-01 00:00:00

abstract：:For a positive coagulase test with certain isolates of Staphylococcus aureus, inoculation of 0.5 ml of coagulase plasma with at least two colonies and 24 h of incubation at 35 to 37 degrees C may be required. S. aureus does not multiply in coagulase plasma. There is also lot-to-lot variation in the sensitivity of coag...

journal_title：Journal of clinical microbiology

authors： Selepak ST,Witebsky FG

更新日期：1985-11-01 00:00:00

abstract：:Twenty-two isolates of St. Louis encephalitis (SLE) virus of various geographical origins (Brazil, Argentina, Panama, Texas, Missouri, Maryland, California, and Florida) were examined for genetic variation by the base excision sequence scanning (BESS T-scan) method. A fragment was amplified in the envelope gene with t...

journal_title：Journal of clinical microbiology

authors： Charrel RN,Lévy N,Tesh RB,Chandler LJ

更新日期：1999-06-01 00:00:00

abstract：:We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalacti...

journal_title：Journal of clinical microbiology

authors： Creti R,Fabretti F,Orefici G,von Hunolstein C

更新日期：2004-03-01 00:00:00

abstract：:The predominant Helicobacter pylori strains circulating among geographic locations differ in regard to genomic structure. The association of the cagA-positive, vacA s1 genotypes with peptic ulcer disease (PUD) and gastric cancer was reported in Western countries but not in East Asian countries. Strains from Western co...

journal_title：Journal of clinical microbiology

authors： Saribasak H,Salih BA,Yamaoka Y,Sander E

更新日期：2004-04-01 00:00:00

abstract：:Hepatitis B surface antigen was concentrated and purified from plasma by two simple steps of purification. In the first step the antigen was purified 24-fold by polyethylene glycol precipitation. An additional 10-fold purification was achieved by batchwise adsorption to hydroxylapatite and subsequent elution with 0.02...

journal_title：Journal of clinical microbiology

authors： Vnek J,Prince AM

更新日期：1976-06-01 00:00:00

abstract：:The incidence of vancomycin resistance among enterococci is increasing in the United States and elsewhere in the world, but automated susceptibility testing methods have difficulty detecting resistance expressed by certain strains. The agar screening method described by Willey et al. (B. M. Willey, B. N. Kreiswirth, A...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Clark NC,Ferraro MJ,Sahm DF,Doern G,Pfaller MA,Reller LB,Weinstein MP,Zabransky RJ,Tenover FC

更新日期：1994-07-01 00:00:00

abstract：:There are no previous reports of human infection due to Acinetobacter baylyi. In this study, we report on six patients with bacteremia due to A. baylyi, based on analysis of the 16S-23S rRNA intergenic spacer and the 16S rRNA gene. All six patients had multiple underlying diseases. The infection was nosocomially acqui...

journal_title：Journal of clinical microbiology

authors： Chen TL,Siu LK,Lee YT,Chen CP,Huang LY,Wu RC,Cho WL,Fung CP

更新日期：2008-09-01 00:00:00

abstract：:We described the molecular epidemiology of expanded-spectrum cephalosporin-resistant gram-negative bacilli (RGN) recovered from inanimate surfaces. RGN were isolated from 9% of environmental cultures. Numerous species, each with multiple unique strains, were recovered. Epidemiological links between environmental, pers...

journal_title：Journal of clinical microbiology

authors： D'Agata EM,Venkataraman L,DeGirolami P,Samore M

更新日期：1999-09-01 00:00:00

abstract：:In this study, the optimal combination of three commercial glycoprotein G-2 (gG-2)-based herpes simplex virus type 2 (HSV-2) type-specific enzyme-linked immunosorbent assays (Euroimmun anti-HSV-2 immunoglobulin G [IgG] ELISA [Eu2], Gull HSV-2-specific IgG ELISA [Gu2], and Radim HSV-2 IgG ELISA [Ra2]) and one gG-2-base...

journal_title：Journal of clinical microbiology

authors： Eing BR,Lippelt L,Lorentzen EU,Hafezi W,Schlumberger W,Steinhagen K,Kühn JE

更新日期：2002-02-01 00:00:00

abstract：:Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s. Here we reassessed the capacity of PCR-restriction enzyme pattern analysis (PRA) of the hsp65 gene to differentiate Nocardia species, including 36 new species. Ou...

journal_title：Journal of clinical microbiology

authors： Rodríguez-Nava V,Couble A,Devulder G,Flandrois JP,Boiron P,Laurent F

更新日期：2006-02-01 00:00:00

abstract：:The increasing incidence of invasive aspergillosis, a life-threatening infection in immunocompromised patients, emphasizes the need to improve the diagnostic tools for this disease. We established a LightCycler-based real-time PCR assay to detect and quantify rapidly, specifically, and sensitively Aspergillus fumigatu...

journal_title：Journal of clinical microbiology

authors： Spiess B,Buchheidt D,Baust C,Skladny H,Seifarth W,Zeilfelder U,Leib-Mösch C,Mörz H,Hehlmann R

更新日期：2003-05-01 00:00:00

abstract：:Eleven reference and 25 clinical isolates of Fusarium were subject to multilocus DNA sequence analysis to determine the species and haplotypes of the fusarial isolates from Beijing and Shandong, China. Seven loci were analyzed: the translation elongation factor 1 alpha gene (EF-1α); the nuclear rRNA internal transcrib...

journal_title：Journal of clinical microbiology

authors： Wang H,Xiao M,Kong F,Chen S,Dou HT,Sorrell T,Li RY,Xu YC

更新日期：2011-05-01 00:00:00

abstract：:Chlamydia pneumoniae is an important human respiratory pathogen. Classification of C. pneumoniae isolates into distinguishable serovars or genotypes has not yet been reported. To determine whether antigenic or molecular variants among C. pneumoniae isolates exist, six strains were studied via immunoblot analysis and D...

journal_title：Journal of clinical microbiology

authors： Jantos CA,Heck S,Roggendorf R,Sen-Gupta M,Hegemann JH

更新日期：1997-03-01 00:00:00

abstract：:In order to determine whether mixed infections of human herpesvirus 6B (HHV-6B) occur in immunocompetent and immunocompromised individuals, we examined the copy numbers of telomeric repeat sequences (TRS) of clinical isolates. In clinical isolates obtained from patients with exanthem subitum caused by primary HHV-6B i...

journal_title：Journal of clinical microbiology

authors： Kato Y,Ihira M,Umeda M,Higashimoto Y,Kawamura Y,Ohashi M,Ishi J,Yoshikawa T

更新日期：2014-02-01 00:00:00

abstract：:Despite the high virological response rates achieved with current directly acting antiviral agents (DAAs) against hepatitis C virus (HCV), around 2% to 5% of treated patients do not achieve a sustained viral response. The identification of amino acid substitutions associated with treatment failure requires analytical ...

journal_title：Journal of clinical microbiology

authors： Soria ME,García-Crespo C,Martínez-González B,Vázquez-Sirvent L,Lobo-Vega R,de Ávila AI,Gallego I,Chen Q,García-Cehic D,Llorens-Revull M,Briones C,Gómez J,Ferrer-Orta C,Verdaguer N,Gregori J,Rodríguez-Frías F,Buti M,Este

更新日期：2020-11-18 00:00:00

abstract：:Ectromelia virus, an orthopoxvirus that can cause extensive morbidity and mortality (mousepox) in colonized mice, has been epizootically responsible for serious disruption of biomedical research since 1930. The lack of a sensitive and specific serological assay for infection with this virus became apparent during outb...

journal_title：Journal of clinical microbiology

authors： Buller RM,Bhatt PN,Wallace GD

更新日期：1983-11-01 00:00:00

abstract：:A set of 104 isolates from human clinical samples from the United States, morphologically compatible with Bipolaris, were morphologically and molecularly identified through the sequence analysis of the internal transcribed space (ITS) region of the nuclear ribosomal DNA (rDNA). The predominant species was Bipolaris sp...

journal_title：Journal of clinical microbiology

authors： da Cunha KC,Sutton DA,Fothergill AW,Cano J,Gené J,Madrid H,De Hoog S,Crous PW,Guarro J

更新日期：2012-12-01 00:00:00

abstract：:Rapid detection of drug resistance in Mycobacterium tuberculosis is essential for efficient treatment and control of this pathogen. The amplification refractory mutation system (ARMS) was used to detect mutations in the rifampin resistance-determining region of the rpoB gene. A total of 39 rifampin-resistant M. tuberc...

journal_title：Journal of clinical microbiology

authors： Fan XY,Hu ZY,Xu FH,Yan ZQ,Guo SQ,Li ZM

更新日期：2003-03-01 00:00:00

abstract：:A mass spectrometry (MS) method that detects a serum disaccharide (DS) (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this assay. Patients with the following IFI were selected according to the availability of sera obtained...

journal_title：Journal of clinical microbiology

authors： Cornu M,Sendid B,Mery A,François N,Mikulska M,Letscher-Bru V,De Carolis E,Damonti L,Titecat M,Bochud PY,Alanio A,Sanguinetti M,Viscoli C,Herbrecht R,Guerardel Y,Poulain D

更新日期：2019-04-26 00:00:00

abstract：:The genetic variation within a bacterial clone (known as Shigella sonnei but in effect a clone of Escherichia coli) was examined by studying 46 clinical isolates that were epidemiologically unassociated and isolated from patients in different countries over a period of 41 years (1950 to 1991). Restriction fragment len...

journal_title：Journal of clinical microbiology

authors： Karaolis DK,Lan R,Reeves PR

更新日期：1994-03-01 00:00:00

abstract：:A modified CAMP test was used to identify 973 Vibrio cholerae isolates by phenotype. Eltor and non-O1 strains were CAMP positive; classical strains were CAMP negative. Sausage-shaped zones of hemolysis of eltor strains were easily distinguished from narrower bands of non-O1 isolates. For O1 isolates, there was 100% ag...

journal_title：Journal of clinical microbiology

authors： Lesmana M,Subekti D,Tjaniadi P,Pazzaglia G

更新日期：1994-01-01 00:00:00

abstract：:We report a case of septic arthritis of a native knee joint due to Corynebacterium striatum, a rare and unusual cause of septic arthritis of native joints. The isolate was identified by a combination of phenotypic, mass spectrometric, and nucleic acid-based assays and exhibited high-level resistance to most antimicrob...

journal_title：Journal of clinical microbiology

authors： Westblade LF,Shams F,Duong S,Tariq O,Bulbin A,Klirsfeld D,Zhen W,Sakaria S,Ford BA,Burnham CA,Ginocchio CC

更新日期：2014-05-01 00:00:00

abstract：:We compared 75 nontypeable (NT) Haemophilus influenzae isolates by pulsed-field gel electrophoresis (PFGE), enterobacterial repetitive intergenic consensus (ERIC)-PCR, and automated ribotyping. PFGE was the most discriminatory of the techniques. ERIC-PCR provides a useful screen but should not replace other techniques...

journal_title：Journal of clinical microbiology

authors： Pettigrew MM,Foxman B,Ecevit Z,Marrs CF,Gilsdorf J

更新日期：2002-02-01 00:00:00

abstract：:The ability of Yersinia to digest polypectate may be of some value in differentiating Y. enterocolitica and Y. pseudotuberculosis from some of the other fermenting gram-negative bacilli, such as Enterobacter agglomerans, with which they can be confused. Pectolytic activity in Yersinia may also have some teleologic or ...

journal_title：Journal of clinical microbiology

authors： von Riesen VL

更新日期：1975-12-01 00:00:00

abstract：:PCR of a Candida albicans cytochrome P-450 lanosterol-alpha-demethylase (P450-L1A1) gene segment is a rapid and sensitive method of detection in clinical specimens. This enzyme is a target for azole antifungal action. In order to directly detect and identify the clinically most important species of Candida, we cloned ...

journal_title：Journal of clinical microbiology

authors： Burgener-Kairuz P,Zuber JP,Jaunin P,Buchman TG,Bille J,Rossier M

更新日期：1994-08-01 00:00:00

abstract：:Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, including cultured HIV-1 isol...

journal_title：Journal of clinical microbiology

authors： Shafer RW,Winters MA,Mayers DL,Japour AJ,Kuritzkes DR,Weislow OS,White F,Erice A,Sannerud KJ,Iversen A,Pena F,Dimitrov D,Frenkel LM,Reichelderfer PS

更新日期：1996-07-01 00:00:00

abstract：:Quantitative PCR on nasopharyngeal aspirate (NPA) can achieve high sensitivity and specificity in diagnosing Pneumocystis pneumonia (PCP) compared to microscopic examination of bronchoscopic specimens in a population with low HIV prevalence. Since NPA is a minimally invasive procedure, it is ideal as a screening test ...

journal_title：Journal of clinical microbiology

authors： To KK,Wong SC,Xu T,Poon RW,Mok KY,Chan JF,Cheng VC,Chan KH,Hung IF,Yuen KY

更新日期：2013-05-01 00:00:00

abstract：:The subgroups and serotypes of 178 strains of rotavirus isolated from diarrheic and healthy children in Bangui, Central African Republic, during a 27-month period were determined by enzyme-linked immunosorbent assay. The subgroup was determined for 152 of the viral strains, 18.4% being subgroup I and 81.6% being subgr...

journal_title：Journal of clinical microbiology

authors： Georges-Courbot MC,Beraud AM,Beards GM,Campbell AD,Gonzalez JP,Georges AJ,Flewett TH

更新日期：1988-04-01 00:00:00