Abstract:
:The subgroups and serotypes of 178 strains of rotavirus isolated from diarrheic and healthy children in Bangui, Central African Republic, during a 27-month period were determined by enzyme-linked immunosorbent assay. The subgroup was determined for 152 of the viral strains, 18.4% being subgroup I and 81.6% being subgroup II. Of the 143 strains which could be serotyped, 71.3% were serotype 1, 15.4% were serotype 2, and 13.3% were serotype 3. Serotypes 1 and 3 were detected throughout the study, while serotype 2 was detected only during 8 months. No serotype exhibited any special epidemiological properties. The serotypes were found to consist of three different electrophoretypes, two long ones (A and B) and a short one (C). All subgroup I, serotype 2 strains presented short electrophoretypes. Strains with identical long electrophoretypes A were either serotype 1 or serotype 3.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Georges-Courbot MC,Beraud AM,Beards GM,Campbell AD,Gonzalez JP,Georges AJ,Flewett THdoi
10.1128/JCM.26.4.668-671.1988subject
Has Abstractpub_date
1988-04-01 00:00:00pages
668-71issue
4eissn
0095-1137issn
1098-660Xjournal_volume
26pub_type
杂志文章abstract::Conventional culture techniques are limited in the ability to detect multiple Streptococcus pneumoniae serotypes in nasopharyngeal (NP) secretions. We developed an immunoblot (IB) method with monoclonal antibodies (MAbs) to detect S. pneumoniae and to identify serotypes. NP specimens stored in skim milk-tryptone-gluco...
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