Rapid detection of Gram-negative bacteriuria by Limulus amoebocyte lysate assay.

abstract：:The DNA fingerprint profiles and serotypes of 63 avian Pasteurella multocida field isolates, 13 attenuated vaccine isolates (propagated from vaccines manufactured by five companies), and 16 somatic reference strains were compared. DNA fingerprinting established the relationship of isolates that could not be distinguis...

journal_title：Journal of clinical microbiology

authors： Wilson MA,Morgan MJ,Barger GE

更新日期：1993-02-01 00:00:00

abstract：:The potential clinical value of colony counts determined by the lysis-centrifugation blood culture method was studied by reviewing the records of eight patients with persistent septicemia in whom colony counts were available on at least 3 days. Colony counts of the five patients who survived decreased steadily as the ...

journal_title：Journal of clinical microbiology

authors： Whimbey E,Wong B,Kiehn TE,Armstrong D

更新日期：1984-06-01 00:00:00

abstract：:The pandemic spread of Vibrio parahaemolyticus is an international public health issue. Because of the outbreak potential of the organism, it is critical to establish an internationally recognized molecular subtyping protocol for V. parahaemolyticus that is both rapid and robust as a means to monitor its further sprea...

journal_title：Journal of clinical microbiology

authors： Kam KM,Luey CK,Parsons MB,Cooper KL,Nair GB,Alam M,Islam MA,Cheung DT,Chu YW,Ramamurthy T,Pazhani GP,Bhattacharya SK,Watanabe H,Terajima J,Arakawa E,Ratchtrachenchai OA,Huttayananont S,Ribot EM,Gerner-Smidt P,Swamin

更新日期：2008-08-01 00:00:00

abstract：:Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399). ...

journal_title：Journal of clinical microbiology

authors： Twin J,Taylor N,Garland SM,Hocking JS,Walker J,Bradshaw CS,Fairley CK,Tabrizi SN

更新日期：2011-03-01 00:00:00

abstract：:The aims of this study were to compare a novel PCR-based Chlamydia trachomatis detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available C. trachomatis detection Hybrid Capture 2 (HC2) assay and to investigate the C. trachomatis serovar distribution among young women in a rural Costa Rican s...

journal_title：Journal of clinical microbiology

authors： Quint K,Porras C,Safaeian M,González P,Hildesheim A,Quint W,van Doorn LJ,Silva S,Melchers W,Schiffman M,Rodríguez AC,Wacholder S,Freer E,Cortes B,Herrero R,Costa Rican Vaccine Trial Group.

更新日期：2007-12-01 00:00:00

abstract：:Genotyping and subtyping of 225 samples with hepatitis C virus (HCV) genotype 1, 2, 3, or 6 infection were done with Versant LiPA 2.0 and Abbott RealTime HCV Genotype (GT) II by using direct sequencing of the NS5B and 5' untranslated regions as the reference standards. The concordance rates were >99.2% for genotypes a...

journal_title：Journal of clinical microbiology

authors： Liu CH,Liang CC,Liu CJ,Lin CL,Su TH,Yang HC,Chen PJ,Chen DS,Kao JH

更新日期：2015-05-01 00:00:00

abstract：:The rapid identification of blood culture isolates and antimicrobial susceptibility test (AST) results play critical roles for the optimal treatment of patients with bloodstream infections. Whereas others have looked at the time to detection in automated culture systems, we examined the overall time from specimen coll...

journal_title：Journal of clinical microbiology

authors： Tabak YP,Vankeepuram L,Ye G,Jeffers K,Gupta V,Murray PR

更新日期：2018-11-27 00:00:00

abstract：:In five separate fecal collections spanning three years, group A rotaviruses were detected by enzyme-linked immunosorbent assay in 35 (25%) of 142 specimens obtained from nondiarrheic, hospitalized neonates in Blantyre, Malawi. Molecular characterization of each strain identified, for the first time in neonates, a sho...

journal_title：Journal of clinical microbiology

authors： Cunliffe NA,Rogerson S,Dove W,Thindwa BD,Greensill J,Kirkwood CD,Broadhead RL,Hart CA

更新日期：2002-04-01 00:00:00

abstract：:Epstein-Barr virus (EBV) infection is associated with a broad spectrum of disease. While quantification of EBV nucleic acid in the peripheral blood has been demonstrated to be useful for diagnosis and patient care, the optimal sample type and reporting format for such testing remain uncertain. Using quantitative real-...

journal_title：Journal of clinical microbiology

authors： Hakim H,Gibson C,Pan J,Srivastava K,Gu Z,Bankowski MJ,Hayden RT

更新日期：2007-07-01 00:00:00

abstract：:A replicator method for screening Staphylococcus aureus isolates for synergistic hemolytic activity is described. The technique should also be useful for assessing the hemolytic activity of staphylococci and streptococci on different lots or brands of blood agar because the inoculum and the distances between spot inoc...

journal_title：Journal of clinical microbiology

authors： Boyce JM

更新日期：1985-05-01 00:00:00

abstract：:White-footed mice, Peromyscus leucopus, were captured in southern Connecticut during 1997 and 1998 to determine the prevalence of infections caused by granulocytic Ehrlichia sp., Borrelia burgdorferi, and Babesia microti. Of the 50 mice captured and recaptured, 25 of 47 (53.2%) and 23 of 48 (47.9%) contained antibodie...

journal_title：Journal of clinical microbiology

authors： Stafford KC 3rd,Massung RF,Magnarelli LA,Ijdo JW,Anderson JF

更新日期：1999-09-01 00:00:00

abstract：:The single-nucleotide variation 823C to T (His275Tyr), responsible for oseltamivir drug resistance has been detected in some isolates of the influenza A/H1N1/2009 virus. Early detection of the presence of this oseltamivir-resistant strain allows prompt consideration of alternative treatment options. An isolated-probe-...

journal_title：Journal of clinical microbiology

authors： Lee HK,Lee CK,Loh TP,Tang JW,Tambyah PA,Koay ES

更新日期：2011-10-01 00:00:00

abstract：:Examination of a number of previously published aligned Borrelia 16S rRNA sequences revealed the presence of regions which could serve as oligonucleotide probe targets for both species-specific identification of Borrelia burgdorferi and distinction between genomic groups. Total cellular RNA isolated from Borrelia cult...

journal_title：Journal of clinical microbiology

authors： Marconi RT,Lubke L,Hauglum W,Garon CF

更新日期：1992-03-01 00:00:00

abstract：:The API STAPH-IDENT system was compared with conventional methods for the identification of 14 Staphylococcus species. Conventional methods included the Kloos and Schleifer simplified scheme and DNA-DNA hybridization. The API STAPH-IDENT strip utilizes a battery of 10 miniaturized biochemical tests, including alkaline...

journal_title：Journal of clinical microbiology

authors： Kloos WE,Wolfshohl JF

更新日期：1982-09-01 00:00:00

abstract：:Multilocus sequence typing was successfully completed on 494 isolates of Streptococcus uberis from clinical mastitis cases in a study of 52 commercial dairy herds over a 12-month period. In total, 195 sequence types (STs) were identified. S. uberis mastitis cases that occurred in different cows within the same herd an...

journal_title：Journal of clinical microbiology

authors： Davies PL,Leigh JA,Bradley AJ,Archer SC,Emes RD,Green MJ

更新日期：2016-01-01 00:00:00

abstract：:An immunofluorescence assay (IFA) for the detection of immunoglobulin G antibodies directed against Helicobacter pylori was evaluated by comparing 20 serum specimens from patients with a positive urease test on biopsy material and 20 serum specimens from patients with a negative test and with defined clinical symptoms...

journal_title：Journal of clinical microbiology

authors： Faulde M,Putzker M,Mertes T,Sobe D

更新日期：1991-02-01 00:00:00

abstract：:Lyme borreliosis is a tick-borne disease caused by the Borrelia burgdorferisensu lato complex. Bio-Rad Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B. burgdorferi The BioPlex 2200 Lyme Total assay exhibits an improved rate of seropositivity in p...

journal_title：Journal of clinical microbiology

authors： Hahm JB,Breneman JW 4th,Liu J,Rabkina S,Zheng W,Zhou S,Walker RP,Kaul R

更新日期：2020-04-23 00:00:00

abstract：:As part of an ongoing investigation into nosocomial Legionella infections at Stanford University Medical Center (SUMC), we applied the technique of restriction endonuclease analysis (REA) to determine strain differences among three species, including Legionella pneumophila, Legionella dumoffii, and Legionella micdadei...

journal_title：Journal of clinical microbiology

authors： Tompkins LS,Troup NJ,Woods T,Bibb W,McKinney RM

更新日期：1987-10-01 00:00:00

abstract：:Continuous cell lines were tested for their potential use in distinguishing pathogenic from nonpathogenic Listeria species. Listeria monocytogenes and Listeria ivanovii strains were lethal for mice, and culture filtrates were cytotoxic for cultured cells and hemolytic for sheep erythrocytes, while Listeria innocua, Li...

journal_title：Journal of clinical microbiology

authors： Farber JM,Speirs JI

更新日期：1987-08-01 00:00:00

abstract：:An outbreak of pseudobacteremia due to Streptococcus pyogenes (group A streptococci [GAS]) and methicillin-susceptible Staphylococcus aureus (MSSA) was traced to the venting procedure for aerobic bottles prior to their loading into the incubator of the BacT/Alert analyzer (Organon Teknika). Bacteria shed by a laborato...

journal_title：Journal of clinical microbiology

authors： Simhon A,Rahav G,Shapiro M,Block C

更新日期：2001-01-01 00:00:00

abstract：:Bordetella pertussis is a Gram-negative bacterium that causes respiratory infections in humans. Ongoing molecular surveillance of B. pertussis acellular vaccine (aP) antigens is critical for understanding the interaction between evolutionary pressures, disease pathogenesis, and vaccine effectiveness. Methods currently...

journal_title：Journal of clinical microbiology

authors： Marchand-Austin A,Tsang RSW,Guthrie JL,Ma JH,Lim GH,Crowcroft NS,Deeks SL,Farrell DJ,Jamieson FB

更新日期：2017-05-01 00:00:00

abstract：:We present the first case of severe pneumonia possibly caused by Leptotrichia species with oral bacteria. This was found in a healthy but elderly subject whose bronchoalveolar lavage fluid was analyzed by 16S rRNA gene sequencing analysis. The combination of this method and microscopic observation provided useful info...

journal_title：Journal of clinical microbiology

authors： Kawanami T,Fukuda K,Yatera K,Kido T,Yoshii C,Taniguchi H,Kido M

更新日期：2009-02-01 00:00:00

abstract：:By using primers based on the sequence of a species-specific antigen of Helicobacter pylori (P. O'Toole, S.M. Logan, M. Kostrzynska. T. Wadström, and T.J. Trust, J. Bacteriol. 173:505-513, 1991), a protocol was established for detection of this microorganism in gastric biopsy samples by the polymerase chain reaction (...

journal_title：Journal of clinical microbiology

authors： Hammar M,Tyszkiewicz T,Wadström T,O'Toole PW

更新日期：1992-01-01 00:00:00

abstract：:Several methods have been previously suggested for the presumptive identification of Gardnerella vaginalis in clinical laboratories, but none is entirely satisfactory. We previously found that sodium polyanetholesulfonate (SPS) inhibits G. vaginalis in blood culture media. We compared susceptibility to an SPS-containi...

journal_title：Journal of clinical microbiology

authors： Reimer LG,Reller LB

更新日期：1985-02-01 00:00:00

abstract：:In this study, 113 Enterococcus faecium, 37 Enterococcus faecalis, 24 Enterococcus gallinarum, 15 Enterococcus raffinosus, and 13 Enterococcus casseliflavus clinical isolates and American Type Culture Collection (ATCC) strains were evaluated by contour-clamped homogeneous electric field electrophoresis. Thirty-one of ...

journal_title：Journal of clinical microbiology

authors： Donabedian S,Chow JW,Shlaes DM,Green M,Zervos MJ

更新日期：1995-01-01 00:00:00

abstract：:A total of 53 strains of enterococci, including recently isolated strains with high-level resistance to vancomycin, were tested for vancomycin susceptibility by broth microdilution and disk diffusion using Mueller-Hinton media with and without supplementation with 5% blood. By using currently published parameters of t...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Hill BC,Thornsberry C

更新日期：1989-09-01 00:00:00

abstract：:We have studied 221 adults drawn from an impoverished urban population with high human immunodeficiency virus (HIV) seroprevalence (35%) to determine the prevalence of gastroduodenal pathology and its relationship to serological markers of Helicobacter pylori virulence proteins and other potential environmental and im...

journal_title：Journal of clinical microbiology

authors： Fernando N,Holton J,Zulu I,Vaira D,Mwaba P,Kelly P

更新日期：2001-04-01 00:00:00

abstract：:In a multi-health center study, a new rapid optical immunoassay (OIA) for the detection of Shiga toxin types 1 and 2, the BioStar OIA SHIGATOX kit (Inverness Medical Professional Diagnostics, Inc.), was used to prospectively screen 742 fresh fecal samples for Shiga toxins in parallel with the Premier enterohemorrhagic...

journal_title：Journal of clinical microbiology

authors： Teel LD,Daly JA,Jerris RC,Maul D,Svanas G,O'Brien AD,Park CH

更新日期：2007-10-01 00:00:00

abstract：:Classical microbiological diagnosis of human brucellosis is time-consuming, hazardous, and subject to variable interpretation. Intact-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the routine identification of Brucella spp. Analysis of mass peak patt...

journal_title：Journal of clinical microbiology

authors： Karger A,Melzer F,Timke M,Bettin B,Kostrzewa M,Nöckler K,Hohmann A,Tomaso H,Neubauer H,Al Dahouk S

更新日期：2013-09-01 00:00:00

abstract：:A TaqMan real-time PCR assay, the COBAS TaqMan human immunodeficiency virus (HIV) (HPS-CTMHIV) PCR assay, recently developed for the quantification of HIV type 1 RNA in plasma, was evaluated in comparison with the licensed COBAS AMPLICOR HIV-1 MONITOR (CAHIM) assay. In this study, we have analyzed the tests' sensitivi...

journal_title：Journal of clinical microbiology

authors： Perrin L,Pawlotsky JM,Bouvier-Alias M,Sarrazin C,Zeuzem S,Colucci G

更新日期：2006-12-01 00:00:00