Potential use of continuous cell lines to distinguish between pathogenic and nonpathogenic Listeria spp.

abstract：:HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific f...

journal_title：Journal of clinical microbiology

authors： Estrade C,Sahli R

更新日期：2014-11-01 00:00:00

abstract：:Bacterial vaginosis (BV) remains the most common cause of abnormal vaginal discharge in women of reproductive age and is associated with increased susceptibility to human immunodeficiency virus and sexually transmitted infections and preterm delivery. Present diagnostic methods require access to microscopy and laborat...

journal_title：Journal of clinical microbiology

authors： Bradshaw CS,Morton AN,Garland SM,Horvath LB,Kuzevska I,Fairley CK

更新日期：2005-03-01 00:00:00

abstract：:We developed a blood spot test for syphilis antibody using enzyme-linked immunosorbent assay (ELISA) technology. Dried blood was eluted by buffered saline or, for a supplementary confirmatory test, by treponemal-antibody test diluent. Eluates were diluted in an absorption buffer (Calypte Biomedical, Berkeley, Calif.) ...

journal_title：Journal of clinical microbiology

authors： Stevens R,Pass K,Fuller S,Wiznia A,Noble L,Duva S,Neal M

更新日期：1992-09-01 00:00:00

abstract：:The Echinococcus Western Blot IgG (LDBIO Diagnostics, Lyon, France), using a whole larval antigen from Echinococcus multilocularis, was evaluated for serodiagnosis and differentiation between two human parasitic infections of worldwide importance: cystic echinococcosis, due to Echinococcus granulosus, and alveolar ech...

journal_title：Journal of clinical microbiology

authors： Liance M,Janin V,Bresson-Hadni S,Vuitton DA,Houin R,Piarroux R

更新日期：2000-10-01 00:00:00

abstract：:Gram-negative, anaerobic bacilli are unusual organisms to be isolated in cases of acute septic arthritis. We report the isolation of Fusobacterium necrophorum from joint aspirate in a case of acute septic arthritis, which presented 3 weeks after the drainage of a dental abscess. ...

journal_title：Journal of clinical microbiology

authors： Sonsale PD,Philipson MR,Bowskill J

更新日期：2004-07-01 00:00:00

abstract：:A combination of two systems for the serotyping of Edwardsiella tarda developed independently at the National Institute of Health, Tokyo, Japan, and the Centers for Disease Control, Atlanta, Ga., has enabled a single serotyping scheme comprising 61 O groups and 45 H antigens to be established for international use. ...

journal_title：Journal of clinical microbiology

authors： Tamura K,Sakazaki R,McWhorter AC,Kosako Y

更新日期：1988-11-01 00:00:00

abstract：:A cell-free growth medium for the opportunistic pathogenic ameba Balamuthia mandrillaris is presented. This represents an advance over the use of monkey kidney cells for growth of the amebas and can be helpful in isolation of these amebas from brain tissue from cases in which amebic meningoencephalitis is a diagnostic...

journal_title：Journal of clinical microbiology

authors： Schuster FL,Visvesvara GS

更新日期：1996-02-01 00:00:00

abstract：:Phoenix 100 and Vitek 2 (operating with the current colorimetric cards) are commonly used in hospital laboratories for rapid identification of microorganisms. The present meta-analysis aims to evaluate and compare their performance on Gram-positive and Gram-negative bacteria. The MEDLINE database was searched up to Oc...

journal_title：Journal of clinical microbiology

authors： Chatzigeorgiou KS,Sergentanis TN,Tsiodras S,Hamodrakas SJ,Bagos PG

更新日期：2011-09-01 00:00:00

abstract：:Single multiplexed assays could replace the standard 2-tiered (STT) algorithm recommended for the laboratory diagnosis of Lyme disease if they perform with a specificity and a sensitivity superior or equal to those of the STT algorithm. We used human serum rigorously characterized to be sera from patients with acute- ...

journal_title：Journal of clinical microbiology

authors： Arumugam S,Nayak S,Williams T,di Santa Maria FS,Guedes MS,Chaves RC,Linder V,Marques AR,Horn EJ,Wong SJ,Sia SK,Gomes-Solecki M

更新日期：2019-11-22 00:00:00

abstract：:Chlamydia pneumoniae has proved to be difficult to isolate and propagate in cell culture. We compared the growth of three strains of C. pneumoniae, TW-183 and two clinical isolates from Brooklyn, N.Y., in five cell lines, including HeLa 229, McCoy, HL, HEp-2, and HTED, an immortalized human tracheal cell line. HEp-2 w...

journal_title：Journal of clinical microbiology

authors： Roblin PM,Dumornay W,Hammerschlag MR

更新日期：1992-08-01 00:00:00

abstract：:We present the case of a 77-year-old woman who developed an Actinomyces naeslundii infection of a hip prosthesis. The isolate grew well aerobically with 5% CO(2). Possible diagnostic problems may arise in the microbiological laboratory because aerobic growth is not sufficiently accounted for in some of the traditional...

journal_title：Journal of clinical microbiology

authors： Wüst J,Steiger U,Vuong H,Zbinden R

更新日期：2000-02-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for the detection of antibodies to dengue virus is described. This method correlates well with a hemagglutination inhibition technique. The enzyme-linked immunosorbent assay can also be specific for human immunoglobulin M antibodies when a mu-chain-specific antiglobulin-enzyme conj...

journal_title：Journal of clinical microbiology

authors： Dittmar D,Cleary TJ,Castro A

更新日期：1979-04-01 00:00:00

abstract：:We examined Enterococcus faecalis strains clinically isolated from 100 patients with urinary tract infections (UTIs) for their susceptibility to levofloxacin (LVX) by measuring the MIC and investigated amino acid mutations by direct DNA sequencing, which were then correlated with LVX resistance. Next, we studied risk ...

journal_title：Journal of clinical microbiology

authors： Yasufuku T,Shigemura K,Shirakawa T,Matsumoto M,Nakano Y,Tanaka K,Arakawa S,Kawabata M,Fujisawa M

更新日期：2011-11-01 00:00:00

abstract：:To differentiate the Borrelia burgdorferi sensu lato genospecies, LightCycler real-time PCR was used for the fluorescence (SYBR Green I) melting curve analysis of borrelial recA gene PCR products. The specific melting temperature analyzed is a function of the GC/AT ratio, length, and nucleotide sequence of the amplifi...

journal_title：Journal of clinical microbiology

authors： Pietilä J,He Q,Oksi J,Viljanen MK

更新日期：2000-07-01 00:00:00

abstract：:Buruli ulcer, an infection caused by Mycobacterium ulcerans, is, after tuberculosis and leprosy, the third most common mycobacterial disease. The mode of transmission of M. ulcerans is not exactly known, but since Buruli ulcer often occurs in focalized swampy areas, it is assumed that there is a reservoir of the patho...

journal_title：Journal of clinical microbiology

authors： Rondini S,Mensah-Quainoo E,Troll H,Bodmer T,Pluschke G

更新日期：2003-09-01 00:00:00

abstract：:The timely and exact diagnosis of prosthetic joint infection (PJI) is crucial for surgical decision-making. Intraoperatively, delivery of the result within an hour is required. Alpha-defensin lateral immunoassay of joint fluid (JF) is precise for the intraoperative exclusion of PJI; however, for patients with a limite...

journal_title：Journal of clinical microbiology

authors： Fillerova R,Gallo J,Radvansky M,Kraiczova V,Kudelka M,Kriegova E

更新日期：2017-09-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently been described as a fast and inexpensive method for the identification of mycobacteria. Although mycobacteria require extraction prior to MALDI-TOF MS analysis, previously published protocols have been relatively c...

journal_title：Journal of clinical microbiology

authors： Mather CA,Rivera SF,Butler-Wu SM

更新日期：2014-01-01 00:00:00

abstract：:Therapeutic options for infections caused by gram-negative organisms expressing plasmid-mediated AmpC beta-lactamases are limited because these organisms are usually resistant to all the beta-lactam antibiotics, except for cefepime, cefpirome, and the carbapenems. These organisms are a major concern in nosocomial infe...

journal_title：Journal of clinical microbiology

authors： Pérez-Pérez FJ,Hanson ND

更新日期：2002-06-01 00:00:00

abstract：:Interfering substances have been reported to inhibit PCR assays for the direct detection of Mycobacterium tuberculosis in clinical specimens. Using an internal control, we determined that 52% of respiratory specimens interfered with our PCR assay. On the basis of these findings, we tried to circumvent the problem by s...

journal_title：Journal of clinical microbiology

authors： Forbes BA,Hicks KE

更新日期：1996-09-01 00:00:00

abstract：:We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia s...

journal_title：Journal of clinical microbiology

authors： Qurollo BA,Riggins D,Comyn A,Zewde MT,Breitschwerdt EB

更新日期：2014-11-01 00:00:00

abstract：:The actinomycete Rhodococcus equi is an important pathogen of horses and an emerging opportunistic pathogen of humans. Identification of R. equi by classical bacteriological techniques is sometimes difficult, and misclassification of an isolate is not uncommon. We report here on a specific PCR assay for the rapid and ...

journal_title：Journal of clinical microbiology

authors： Ladrón N,Fernández M,Agüero J,González Zörn B,Vázquez-Boland JA,Navas J

更新日期：2003-07-01 00:00:00

abstract：:Actinomyces viscosus is a gram-positive, non-acid-fact, facultative, catalase-positive, filamentous, or diphtheroidal microorganism. It was isolated from six canine infections during a period of 1.5 years. The organism was cultured from exudate and flaky granules aspirated from infectious granulomas and empyemas. All ...

journal_title：Journal of clinical microbiology

authors： Davenport AA,Carter GR,Patterson MJ

更新日期：1975-01-01 00:00:00

abstract：:Nocardia infection following anterior cruciate ligament (ACL) allograft reconstruction is a rare occurrence. We report a case of Nocardia infection of an allograft ACL reconstruction and septic arthritis of the knee joint due to an organism most similar to the novel Nocardia species Nocardia aobensis. ...

journal_title：Journal of clinical microbiology

authors： Yong EX,Cheong EY,Boutlis CS,Chen DB,Liu EY,McKew GL

更新日期：2015-08-01 00:00:00

abstract：:Examination of a number of previously published aligned Borrelia 16S rRNA sequences revealed the presence of regions which could serve as oligonucleotide probe targets for both species-specific identification of Borrelia burgdorferi and distinction between genomic groups. Total cellular RNA isolated from Borrelia cult...

journal_title：Journal of clinical microbiology

authors： Marconi RT,Lubke L,Hauglum W,Garon CF

更新日期：1992-03-01 00:00:00

abstract：:A clustered regularly interspaced short palindromic repeat (CRISPR) typing method has recently been developed and used for typing and subtyping of Salmonella spp., but it is complicated and labor intensive because it has to analyze all spacers in two CRISPR loci. Here, we developed a more convenient and efficient meth...

journal_title：Journal of clinical microbiology

authors： Li H,Li P,Xie J,Yi S,Yang C,Wang J,Sun J,Liu N,Wang X,Wu Z,Wang L,Hao R,Wang Y,Jia L,Li K,Qiu S,Song H

更新日期：2014-08-01 00:00:00

abstract：:Candida auris is an emerging worldwide fungal pathogen. Over the past 20 years, 61 patient isolates of C. auris (4 blood and 57 ear) have been obtained from 13 hospitals in Korea. Here, we reanalyzed those molecularly identified isolates using two matrix-assisted laser desorption ionization-time of flight mass spectro...

journal_title：Journal of clinical microbiology

authors： Kwon YJ,Shin JH,Byun SA,Choi MJ,Won EJ,Lee D,Lee SY,Chun S,Lee JH,Choi HJ,Kee SJ,Kim SH,Shin MG

更新日期：2019-03-28 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) variant B is frequently identified in peripheral blood, but identification of HHV-6 variant A is relatively rare. We devised a PCR-based method for sensitive, simultaneous detection of both HHV-6 variants. The method was applied to 34 lung tissue specimens that were previously shown to cont...

journal_title：Journal of clinical microbiology

authors： Cone RW,Huang ML,Hackman RC,Corey L

更新日期：1996-04-01 00:00:00

abstract：:A Legionella-like organism (strain 798-PA-H, ATCC 35999) isolated from a lung aspirate was shown by DNA hybridization to belong to the species Legionella hackeliae. Growth, gas-liquid chromatographic, and physiologic characteristics were consistent with those of the type strain of L. hackeliae, Lansing 2 (ATCC 35250)....

journal_title：Journal of clinical microbiology

authors： Wilkinson HW,Thacker WL,Steigerwalt AG,Brenner DJ,Ampel NM,Wing EJ

更新日期：1985-10-01 00:00:00

abstract：:A convenient assay combining solution hybridization and enzyme immunoassay for DNA-RNA hybrids (polymerase chain reaction-enzyme immunoassay [PCR-EIA]) was developed to detect human immunodeficiency virus type 1 (HIV-1) provirus amplified by the PCR and was compared with oligomer hybridization with 32P-labeled SK19. I...

journal_title：Journal of clinical microbiology

authors： Coutlée F,Saint-Antoine P,Olivier C,Voyer H,Kessous-Elbaz A,Berrada F,Bégin P,Giroux L,Viscidi R

更新日期：1991-11-01 00:00:00

abstract：:Infection of calves with bovine herpesvirus 1 (BHV-1) results in transient immunosuppression that may lead to bacterium-induced pneumonia and, occasionally, death. Although sensory neurons in the trigeminal ganglia (TG) are the primary site of BHV-1 latency, viral genomes are detected in the tonsils of latently infect...

journal_title：Journal of clinical microbiology

authors： Perez S,Inman M,Doster A,Jones C

更新日期：2005-01-01 00:00:00