Mechanisms of and risk factors for fluoroquinolone resistance in clinical Enterococcus faecalis isolates from patients with urinary tract infections.

Abstract:

:We examined Enterococcus faecalis strains clinically isolated from 100 patients with urinary tract infections (UTIs) for their susceptibility to levofloxacin (LVX) by measuring the MIC and investigated amino acid mutations by direct DNA sequencing, which were then correlated with LVX resistance. Next, we studied risk factors for LVX resistance, such as age, gender, and previous fluoroquinolone use, and investigated the statistical correlation of these risk factors with each amino acid mutation and LVX resistance. Of the 100 isolates tested, 14 isolates showed LVX resistance and all of these isolates had amino acid mutations. We demonstrated that 2 out of 4 mutations (Ser83-to-Ile in gyrA and Ser80-to-Ile in parC) had a significant correlation with LVX resistance. There was a significant relationship between isolates with 2 or 3 amino acid mutations and LVX resistance. In addition, we found a significant correlation between the previous use of fluoroquinolones and LVX resistance or the presence of mutations and also demonstrated that previous use of other types of antibiotics was significantly related to the presence of mutations by multivariate analysis. In conclusion, we found significant correlation between amino acid mutations in E. faecalis, LVX resistance, and risk factors such as previous use of fluoroquinolones.

journal_name

J Clin Microbiol

authors

Yasufuku T,Shigemura K,Shirakawa T,Matsumoto M,Nakano Y,Tanaka K,Arakawa S,Kawabata M,Fujisawa M

doi

10.1128/JCM.05549-11

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

3912-6

issue

11

eissn

0095-1137

issn

1098-660X

pii

JCM.05549-11

journal_volume

49

pub_type

杂志文章
  • Performance of a polymer-based DNA chip platform in detection and genotyping of human papillomavirus in clinical samples.

    abstract::Human papillomavirus (HPV) plays a key role in the development of cervical and laryngeal cancers. The aim of our study was to compare the performance of a new hydrogel-based HPV genotyping biochip assay (Biochip) to a commercially available and CE-marked conventional PCR followed by reverse hybridization (GenID-PCR). ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02080-08

    authors: Schenk T,Brandstetter T,Zur Hausen A,Alt-Mörbe J,Huzly D,Rühe J

    更新日期:2009-05-01 00:00:00

  • Infectivity of human coronavirus strain 229E.

    abstract::The replication of human coronavirus strain 229E was observed by using indirect immunofluorescence in infected monolayers of MRC continuous cells. By 8 h after infection, bright cytoplasmic fluorescence was detected in cells infected with human coronavirus 229E. Discrete foci of infection were observed from 8 to 16 h ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.12.3.462-468.1980

    authors: Macnaughton MR,Thomas BJ,Davies HA,Patterson S

    更新日期:1980-09-01 00:00:00

  • Comparative Evaluation of Subtyping Tools for Surveillance of Newly Emerging HIV-1 Strains.

    abstract::HIV-1 non-B subtypes/circulating recombinant forms (CRFs) are increasing worldwide. Since subtype identification can be clinically relevant, we assessed the added value in HIV-1 subtyping using updated molecular phylogeny (Mphy) and the performance of routinely used automated tools. Updated Mphy (2015 updated referenc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00656-17

    authors: Fabeni L,Berno G,Fokam J,Bertoli A,Alteri C,Gori C,Forbici F,Takou D,Vergori A,Zaccarelli M,Maffongelli G,Borghi V,Latini A,Pennica A,Mastroianni CM,Montella F,Mussini C,Andreoni M,Antinori A,Perno CF,Santoro MM,

    更新日期:2017-09-01 00:00:00

  • Paratuberculosis infection of nonruminant wildlife in Scotland.

    abstract::Recent reports of natural paratuberculosis (or Johne's disease) in rabbits, foxes, and stoats has focused debate on the presence and importance of wildlife reservoirs in the epidemiology of this disease. This paper describes an extensive study investigating 18 nonruminant wildlife species for evidence of paratuberculo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.4.1517-1521.2001

    authors: Beard PM,Daniels MJ,Henderson D,Pirie A,Rudge K,Buxton D,Rhind S,Greig A,Hutchings MR,McKendrick I,Stevenson K,Sharp JM

    更新日期:2001-04-01 00:00:00

  • Evaluation of an rRNA-derived oligonucleotide probe for culture confirmation of Neisseria gonorrhoeae.

    abstract::The reliability of an rRNA-derived oligonucleotide probe for Neisseria gonorrhoeae was tested with 187 N. gonorrhoeae isolates, 81 Neisseria meningitidis isolates, and several strains of other bacterial species. The probe proved to be 100% specific and 100% sensitive. N. gonorrhoeae cells could also be reliably identi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.5.944-948.1990

    authors: Rossau R,Duhamel M,Van Dyck E,Piot P,Van Heuverswyn H

    更新日期:1990-05-01 00:00:00

  • Evaluation of the pathotec Rapid I-D system for identification of Enterobacteriaceae.

    abstract::The PathoTec Rapid I-D System for identifying Enterobacteriaceae was evaluated with 471 cultures. In 4,910 individual test comparisons, 95.5% of the results agreed, with results of only two test strips, those for esculin hydrolysis and urease production, agreeing with conventional tests in less than 94% of the trials....

    journal_title:Journal of clinical microbiology

    pub_type: 临床试验,杂志文章

    doi:10.1128/JCM.1.4.359-362.1975

    authors: Smith PB,Rhoden DL,Tomfohrde KM

    更新日期:1975-04-01 00:00:00

  • Human infections caused by thiamine- or menadione-requiring Staphylococcus aureus.

    abstract::Stable dwarf forms of Staphylococcus aureus have been identified in clinical specimens as the sole or predominant isolate in eight cases. These organisms have been shown to be menadione or thiamine dependent, i.e., cultivation in the presence of one of these agents has permitted growth of colonies which appear typical...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Acar JF,Goldstein FW,Lagrange P

    更新日期:1978-08-01 00:00:00

  • Population Structure and Antimicrobial Resistance of Canine Uropathogenic Escherichia coli.

    abstract::Escherichia coli is the most common cause of human and canine urinary tract infection (UTI). Clonal groups, often with high levels of antimicrobial resistance, are a major component of the E. coli population that causes human UTI. While little is known about the population structure of E. coli that causes UTI in dogs,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.00788-18

    authors: LeCuyer TE,Byrne BA,Daniels JB,Diaz-Campos DV,Hammac GK,Miller CB,Besser TE,Davis MA

    更新日期:2018-08-27 00:00:00

  • Turnover of nontypable Haemophilus influenzae in the nasopharynges of healthy children.

    abstract::The nasopharyngeal Haemophilus influenzae flora of healthy children in a day care center was analyzed by repeated sampling during 4 winter months. The average carrier rate was 39%, but 74% of the children became colonized at some time during the study. The H. influenzae isolates were identified by capsular type, bioty...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.10.2175-2179.1989

    authors: Trottier S,Stenberg K,Svanborg-Edén C

    更新日期:1989-10-01 00:00:00

  • Multicomponent chimeric antigen for serodiagnosis of canine visceral leishmaniasis.

    abstract::In this work, we describe the assembly of a synthetic gene coding for several antigenic determinants found in different Leishmania infantum antigens. Selected epitopes were derived from the ribosomal proteins LiP2a, LiP2b, and LiP0 and from the histone H2A. The resulting gene was overexpressed in Escherichia coli eith...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.1.58-63.1998

    authors: Soto M,Requena JM,Quijada L,Alonso C

    更新日期:1998-01-01 00:00:00

  • Evaluation of Ebola virus inactivation procedures for Plasmodium falciparum malaria diagnostics.

    abstract::Plasmodium falciparum malaria is highly endemic in the three most affected countries in the current epidemic of Ebola virus disease (EVD) in West Africa. As EVD and malaria are clinically indistinguishable, both remain part of the differential diagnosis of ill travelers from returning from areas of EVD transmission. W...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00165-15

    authors: Lau R,Wang A,Chong-Kit A,Ralevski F,Boggild AK

    更新日期:2015-04-01 00:00:00

  • Characterization of Hafnia alvei by biochemical tests, random amplified polymorphic DNA PCR, and partial sequencing of 16S rRNA gene.

    abstract::Hafnia alvei strains which possess the attachment-effacement gene (eaeA) may have clinical importance as new diarrhea-causing pathogens and should therefore be differentiated from other H. alvei strains. We characterized diarrheal H. alvei strains, which were positive in the PCR test for the eaeA gene, using biochemic...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.9.2372-2376.1995

    authors: Ridell J,Siitonen A,Paulin L,Lindroos O,Korkeala H,Albert MJ

    更新日期:1995-09-01 00:00:00

  • Identification of Enterocytozoon bieneusi spores in respiratory samples from an AIDS patient with a 2-year history of intestinal microsporidiosis.

    abstract::Enterocytozoon bieneusi, a microsporidian parasite, has been recognized since 1985 as an agent of intestinal microsporidiosis leading to malabsorption syndrome, diarrhea, and weight loss in AIDS patients. Recently, however, we have identified E. bieneusi spores in the sputum, bronchoalveolar lavage, and stool samples ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.35.7.1862-1866.1997

    authors: del Aguila C,Lopez-Velez R,Fenoy S,Turrientes C,Cobo J,Navajas R,Visvesvara GS,Croppo GP,Da Silva AJ,Pieniazek NJ

    更新日期:1997-07-01 00:00:00

  • Recurrent osteomyelitis caused by infection with different bacterial strains without obvious source of reinfection.

    abstract::Recurrence of osteomyelitis by the same bacterial strain is well known. We report three patients with a second episode of osteomyelitis at the same site caused by different strains of bacteria from the original. Formerly infected and altered bone surface might present a region of diminished resistance for a new infect...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.44.3.1194-1196.2006

    authors: Uçkay I,Assal M,Legout L,Rohner P,Stern R,Lew D,Hoffmeyer P,Bernard L

    更新日期:2006-03-01 00:00:00

  • Comparative evaluation of three different genotyping methods for investigation of nosocomial outbreaks of Legionnaires' disease in hospitals.

    abstract::The increased incidence of nosocomial Legionnaires' disease in two hospitals prompted investigation of possible environmental sources. In the search for an effective DNA-typing technique for use in hospital epidemiology, the performance and convenience of three methods-SfiI macrorestriction analysis (MRA), amplified f...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Jonas D,Meyer HG,Matthes P,Hartung D,Jahn B,Daschner FD,Jansen B

    更新日期:2000-06-01 00:00:00

  • Analysis with restriction endonucleases recognizing 4- or 5-base-pair sequences of human adenovirus type 3 isolated from ocular diseases in Sapporo, Japan.

    abstract::A total of 49 adenovirus type 3 (Ad3) strains from patients with epidemic keratoconjunctivitis at an eye clinic in Sapporo, Japan, from 1983 to 1986 were classified into either genome type Ad3f (26 strains) or Ad3g (23 strains). By the use of Hinf1 and TaqI restriction endonucleases, they were classified into 11 and 3...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.10.2365-2369.1990

    authors: Itakura S,Aoki K,Sawada H,Shinagawa M

    更新日期:1990-10-01 00:00:00

  • Cellular fatty acid compositions and isoprenoid quinone contents of 23 Legionella species.

    abstract::The cellular fatty acid compositions and ubiquinone contents of 182 Legionella strains representing 23 species were determined by capillary gas-liquid chromatography and reverse-phase high-performance liquid chromatography, respectively. Except for the type strain of Legionella erythra (ATCC 35303T), all Legionella sp...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.3.465-473.1989

    authors: Lambert MA,Moss CW

    更新日期:1989-03-01 00:00:00

  • Coinfection with human herpesvirus 6 variants A and B in lung tissue.

    abstract::Human herpesvirus 6 (HHV-6) variant B is frequently identified in peripheral blood, but identification of HHV-6 variant A is relatively rare. We devised a PCR-based method for sensitive, simultaneous detection of both HHV-6 variants. The method was applied to 34 lung tissue specimens that were previously shown to cont...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.4.877-881.1996

    authors: Cone RW,Huang ML,Hackman RC,Corey L

    更新日期:1996-04-01 00:00:00

  • Cobas-Bact system for identification of members of the family Enterobacteriaceae in 4 h 20 min.

    abstract::The Cobas-Bact (Roche Diagnostics, Basel, Switzerland) new rotor for the identification (ID) in 4 h 20 min of 33 members of the family Enterobacteriaceae to genus and species level was evaluated by testing 444 strains of which 398 belonged to common species and 46 belonged to rare species of Enterobacteriaceae. Each s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.1.61-66.1987

    authors: Wenger A,Tissières LJ,Praplan FR,Kamm WR,Bille J

    更新日期:1987-01-01 00:00:00

  • Genetic and antigenic characterization of rotavirus serotype G9 strains isolated in Australia between 1997 and 2001.

    abstract::Rotavirus serotype G9 is recognized as the most widespread of the emerging serotypes, emerging since 1996 as a frequent cause of severe acute gastroenteritis in children from many countries covering all continents of the world. This study characterized serotype G9 strains collected in three widely separated Australian...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.8.3649-3654.2003

    authors: Kirkwood C,Bogdanovic-Sakran N,Palombo E,Masendycz P,Bugg H,Barnes G,Bishop R

    更新日期:2003-08-01 00:00:00

  • Incidence of and risk factors for symptomatic visceral leishmaniasis among human immunodeficiency virus type 1-infected patients from Spain in the era of highly active antiretroviral therapy.

    abstract::The way in which the extensive use of highly active antiretroviral therapy (HAART) has influenced the incidence of visceral leishmaniasis (VL) among human immunodeficiency type 1 (HIV-1)-infected patients is not yet understood. The present study assessed whether the incidence of symptomatic VL in HIV-infected patients...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.3.762-767.2002

    authors: de La Rosa R,Pineda JA,Delgado J,Macías J,Morillas F,Mira JA,Sánchez-Quijano A,Leal M,Lissen E

    更新日期:2002-03-01 00:00:00

  • A cluster of cases of Escherichia coli O157 infection in a day-care center in a communal settlement (Kibbutz) in Israel.

    abstract::This is the first report in Israel of a cluster of cases of diarrhea associated with the isolation of Escherichia coli O157 in stool samples. E. coli O157:NM (nonmotile), producing verotoxins 1 and 2 was isolated from the stool samples of three infants less than 1 year of age with diarrhea and from a fourth asymptomat...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.2.520-521.1992

    authors: Lerman Y,Cohen D,Gluck A,Ohad E,Sechter I

    更新日期:1992-02-01 00:00:00

  • Prolonged Bartonella bacteremia in cats associated with cat-scratch disease patients.

    abstract::Recent evidence supports a causal relationship between Bartonella (Rochalimaea) henselae, cat-scratch disease (CSD), and bacillary angiomatosis. Cats appear to be the primary reservoir. Blood from 19 cats owned by 14 patients diagnosed with CSD was cultured. Blood samples from cats owned by veterinary students (n = 25...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.12.3245-3251.1995

    authors: Kordick DL,Wilson KH,Sexton DJ,Hadfield TL,Berkhoff HA,Breitschwerdt EB

    更新日期:1995-12-01 00:00:00

  • Reaction of fungal products with amebocyte lysates of the Japanese horseshoe crab, Tachypleus tridentatus.

    abstract::A commercially available endotoxin assay (CS-TAL) employing a chromogenic peptide and an amebocyte lysate from the Japanese horseshoe crab, Tachypleus tridentatus, gave a positive result with aqueous extracts of all 15 strains of Candida albicans and 1 strain each of Candida tropicalis, Cryptococcus neoformans, and a ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.9.1701-1704.1987

    authors: Hodes DS,Heon D,Hass A,Hyatt AC,Hodes HL

    更新日期:1987-09-01 00:00:00

  • Four methods for identification of gram-negative nonfermenting rods: organisms more commonly encountered in clinical specimens.

    abstract::Four commercial kits, Oxi/Ferm (OF), API 20E (AP), Minitek (MT; BBL Microbiology Systems), and Flow N/F (NF), were evaluated, without additional tests, for identification of 258 gram-negative nonfermentative rods. OF and MT were read after 48 h of incubation, and AP and NF were read after both 24 and 48 h of incubatio...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.12.2.271-278.1980

    authors: Appelbaum PC,Stavitz J,Bentz MS,von Kuster LC

    更新日期:1980-08-01 00:00:00

  • Comparison of three commercially available amplification assays, AMP CT, LCx, and COBAS AMPLICOR, for detection of Chlamydia trachomatis in first-void urine.

    abstract::We compared the Gen-Probe transcription-mediated amplification assay (AMP CT), the Abbott LCx assay, and the Roche COBAS AMPLICOR assay for the detection of Chlamydia trachomatis in a mixed population in urine samples. First-void urine, urethral specimens, and cervical specimens in females were obtained from 1,000 pat...

    journal_title:Journal of clinical microbiology

    pub_type: 临床试验,杂志文章

    doi:10.1128/JCM.35.10.2628-2633.1997

    authors: Goessens WH,Mouton JW,van der Meijden WI,Deelen S,van Rijsoort-Vos TH,Lemmens-den Toom N,Verbrugh HA,Verkooyen RP

    更新日期:1997-10-01 00:00:00

  • Prevalence of rotavirus, adenovirus, norovirus, and astrovirus infections and coinfections among hospitalized children in northern France.

    abstract::From January to December 2007, 973 stool specimens were prospectively collected from children hospitalized for gastroenteritis signs or from neonates and premature cases who were born in two French hospital settings in the north of France. They were tested by rapid enzyme immunoassay (EIA) analyses for rotavirus and a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02181-09

    authors: Tran A,Talmud D,Lejeune B,Jovenin N,Renois F,Payan C,Leveque N,Andreoletti L

    更新日期:2010-05-01 00:00:00

  • Usefulness of plasmid profiles for differentiation of Shigella isolates in Bangladesh.

    abstract::We studied the plasmid profiles of 136 Shigella isolates in Bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. Many different plasmid patterns were observed within each species, indicating that many genetically different strains of Shigella are re...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.20.2.300-301.1984

    authors: Tacket CO,Shahid N,Huq MI,Alim AR,Cohen ML

    更新日期:1984-08-01 00:00:00

  • Comparison of the sequences of the internal transcribed spacer regions and PbGP43 genes of Paracoccidioides brasiliensis from patients and armadillos (Dasypus novemcinctus).

    abstract::Paracoccidioides brasiliensis isolates from 10 nine-banded armadillos (Dasypus novemcinctus) were comparable with 19 clinical isolates by sequence analysis of the PbGP43 gene and ribosomal internal transcribed spacer 1 (ITS1) and ITS2 and by random amplified polymorphic DNA. In this original ITS study, eight isolates ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.12.5735-5737.2003

    authors: Hebeler-Barbosa F,Morais FV,Montenegro MR,Kuramae EE,Montes B,McEwen JG,Bagagli E,Puccia R

    更新日期:2003-12-01 00:00:00

  • Comparison of microscan broth microdilution, synergy quad plate agar dilution, and disk diffusion screening methods for detection of high-level aminoglycoside resistance in enterococcus species.

    abstract::We compared the dried MicroScan microdilution panel, Synergy Quad plate agar dilution, and high-potency disk diffusion screening methods for the detection of high-level aminoglycoside resistance in 815 enterococcal bloodstream isolates. Agreement between the three methods was 99% when testing for high-level gentamicin...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.6.2703-2705.2003

    authors: Murdoch DR,Mirrett S,Harrell LJ,Donabedian SM,Zervos MJ,Reller LB

    更新日期:2003-06-01 00:00:00