Analysis with restriction endonucleases recognizing 4- or 5-base-pair sequences of human adenovirus type 3 isolated from ocular diseases in Sapporo, Japan.

abstract：:Seven commercial rotavirus antigen assays were compared with in-house PCR methods for detecting rotavirus in stool specimens. The assay sensitivities were 80% to 100%, while the specificities were 54.3% for one commercial immunochromatographic (ICT) method and 99.4% to 100% for other assays. Thus, except for one comme...

journal_title：Journal of clinical microbiology

authors： Ye S,Lambert SB,Grimwood K,Roczo-Farkas S,Nimmo GR,Sloots TP,Kirkwood CD,Whiley DM

更新日期：2015-01-01 00:00:00

abstract：:The clinical, histological, and bacteriological findings in five cases of acute appendicitis caused by Haemophilus segnis are reported. This is the first documentation of appendicitis associated with this organism. ...

journal_title：Journal of clinical microbiology

authors： Welch WD,Southern PM Jr,Schneider NR

更新日期：1986-11-01 00:00:00

abstract：:The detection and quantitation of human parainfluenza virus type 1 (HPIV-1) RNA in nasal wash specimens from 49 children with lower respiratory infections were performed by a reverse transcription-PCR-enzyme hybridization assay (RT-PCR-EHA). The HPIV-1 RT-PCR-EHA was then used to test 40 samples from asymptomatic chil...

journal_title：Journal of clinical microbiology

authors： Fan J,Henrickson KJ

更新日期：1996-08-01 00:00:00

abstract：:Blood culture records from 1994 to 1995 from five U.S. medical centers all using the Difco ESP continuous monitoring blood culture system were reviewed retrospectively. Among a total of 7,362 isolates of bacteria and yeasts, only 0.1% of possibly significant isolates would have been missed had blood cultures been rout...

journal_title：Journal of clinical microbiology

authors： Doern GV,Brueggemann AB,Dunne WM,Jenkins SG,Halstead DC,McLaughlin JC

更新日期：1997-05-01 00:00:00

abstract：:A cytopathic astrovirus was isolated from pigs with acute diarrhea in an established cell line that was derived from porcine embryonic kidneys with the aid of trypsin. The virus showed a distinct cytopathic effect characterized by an enlargement of cells and the appearance of fine granules in the cytoplasm. Porcine as...

journal_title：Journal of clinical microbiology

authors： Shimizu M,Shirai J,Narita M,Yamane T

更新日期：1990-02-01 00:00:00

abstract：:We present the case of a 77-year-old woman who developed an Actinomyces naeslundii infection of a hip prosthesis. The isolate grew well aerobically with 5% CO(2). Possible diagnostic problems may arise in the microbiological laboratory because aerobic growth is not sufficiently accounted for in some of the traditional...

journal_title：Journal of clinical microbiology

authors： Wüst J,Steiger U,Vuong H,Zbinden R

更新日期：2000-02-01 00:00:00

abstract：:Sixteen metabolically deficient dwarf-colony mutants of Escherichia coli were isolated from urine culture and represented about 2% of all E. coli isolated during the same period. In 14 cases, mutants were isolated from debilitated patients: elderly persons or patients in the terminal stages of a chronic disease. In 15...

journal_title：Journal of clinical microbiology

authors： Borderon E,Horodniceanu T

更新日期：1978-12-01 00:00:00

abstract：:A colony blot enzyme-linked immunosorbent assay was developed for the rapid identification of smooth Brucella species, i.e., Brucella abortus, B. melitensis, and B. suis. Bacterial colonies from plates were blotted onto nitrocellulose disks, lysed by immersion in chloroform, and reacted with BRU 38, a rat monoclonal a...

journal_title：Journal of clinical microbiology

authors： Roop RM 2nd,Preston-Moore D,Bagchi T,Schurig GG

更新日期：1987-11-01 00:00:00

abstract：:The accuracy of disk susceptibility tests with ampicillin and ampicillin-sulbactam was not improved when the amount of ampicillin was increased from 10 to 20 or 30 micrograms per disk. For testing members of the family Enterobacteriaceae, ampicillin disk tests correlated better with broth microdilution tests when the ...

journal_title：Journal of clinical microbiology

authors： Barry AL,Jones RN

更新日期：1988-04-01 00:00:00

abstract：:The causes of sarcoidosis are unknown. In this study, we report the presence of Mycobacterium tuberculosis complex and Propionibacterium granulosum DNA in a significant proportion of Greek patients with sarcoidosis. Human herpesvirus 8 DNA was not detected in sarcoid tissues from Greek patients. Our findings are discu...

journal_title：Journal of clinical microbiology

authors： Gazouli M,Ikonomopoulos J,Trigidou R,Foteinou M,Kittas C,Gorgoulis V

更新日期：2002-08-01 00:00:00

abstract：:Variability of disease manifestations has been noted in patients with Lyme disease. A contributing factor to this variation may be the number of spirochetes present in infected patients. We evaluated clinical and laboratory findings for patients with erythema migrans with regard to the number of Borrelia burgdorferi o...

journal_title：Journal of clinical microbiology

authors： Liveris D,Wang G,Girao G,Byrne DW,Nowakowski J,McKenna D,Nadelman R,Wormser GP,Schwartz I

更新日期：2002-04-01 00:00:00

abstract：:The recent Zaire Ebola virus (EBOV) outbreak in West Africa illustrates clearly the need for additional studies with humans and animals to elucidate the ecology of Ebola viruses (EBVs). In this study, we developed a serological assay based on the Luminex technology. Nine recombinant proteins representing different vir...

journal_title：Journal of clinical microbiology

authors： Ayouba A,Touré A,Butel C,Keita AK,Binetruy F,Sow MS,Foulongne V,Delaporte E,Peeters M

更新日期：2016-12-28 00:00:00

abstract：:The efficacy of antiviral treatment for chronic hepatitis C virus (HCV) infection is determined by measuring HCV RNA at specific time points throughout therapy using highly sensitive and accurate HCV RNA assays. This study compared the performances of two recently developed real-time PCR HCV RNA assays, cobas HCV for ...

journal_title：Journal of clinical microbiology

authors： Vermehren J,Stelzl E,Maasoumy B,Michel-Treil V,Berkowski C,Marins EG,Paxinos EE,Marino E,Wedemeyer H,Sarrazin C,Kessler HH

更新日期：2017-04-01 00:00:00

abstract：:Surveillance methods that measure St. Louis encephalitis (SLE) virus activity in nature may provide forewarning of its epidemic occurrence in humans. An antigen capture enzyme immunoassay was developed to detect SLE virus in infected mosquitoes. The assay detected purified SLE viral antigen at a concentration of 62 pg...

journal_title：Journal of clinical microbiology

authors： Tsai TF,Bolin RA,Montoya M,Bailey RE,Francy DB,Jozan M,Roehrig JT

更新日期：1987-02-01 00:00:00

abstract：:For prevention and control of gonorrhea, an objective, highly discriminating, and reproducible molecular epidemiological characterization of Neisseria gonorrhoeae is essential. In the present study, in pursuance of providing such qualities, pyrosequencing technology, a fast real-time DNA sequence analysis, was applied...

journal_title：Journal of clinical microbiology

authors： Unemo M,Olcén P,Jonasson J,Fredlund H

更新日期：2004-07-01 00:00:00

abstract：:A PCR for protein D (hpd#3) was used to differentiate nontypeable Haemophilus influenzae (NTHI) from Haemophilus haemolyticus. While 90% of nasopharyngeal specimens and 100% of lower-airway specimens from 84 Indigenous Australian children with bronchiectasis had phenotypic NTHI isolates confirmed as H. influenzae, onl...

journal_title：Journal of clinical microbiology

authors： Hare KM,Binks MJ,Grimwood K,Chang AB,Leach AJ,Smith-Vaughan H

更新日期：2012-07-01 00:00:00

abstract：:Lyme disease prevails as the most commonly transmitted tick-borne infection in the United States, and serologic evaluation for antibodies to Borrelia burgdorferi remains the recommended modality for diagnosis. This review presents a brief historical perspective on the evolution of serologic assays for Lyme disease and...

journal_title：Journal of clinical microbiology

authors： Theel ES

更新日期：2016-05-01 00:00:00

abstract：:By the use of enzyme immunoassay and RNA electrophoresis, rotavirus serotyping was performed with stool specimens from 605 children. Serotypes 1, 2, 3, and 4 accounted for 63, 9, 9, and 19% of the cases, respectively, with considerable yearly variation. An average of three serotypes cocirculated each year. ...

journal_title：Journal of clinical microbiology

authors： Begue RE,Dennehy PH,Huang J,Martin P

更新日期：1992-06-01 00:00:00

abstract：:The IDI-MRSA assay (Infectio Diagnostic, Inc., Sainte-Foy, Quebec, Canada) with the Smart Cycler II rapid DNA amplification system (Cepheid, Sunnyvale, CA) appears to be sensitive and specific for the rapid detection of nasal colonization by methicillin-resistant Staphylococcus aureus (MRSA). We assessed the sensitivi...

journal_title：Journal of clinical microbiology

authors： Bishop EJ,Grabsch EA,Ballard SA,Mayall B,Xie S,Martin R,Grayson ML

更新日期：2006-08-01 00:00:00

abstract：:Serologic methods are an important part of a clinical laboratory's portfolio of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) tests and are essential to the broader response to coronavirus infectious disease 2019 (COVID-19), including epidemiological studies and vaccine development. There are currently ...

journal_title：Journal of clinical microbiology

authors： Loeffelholz MJ

更新日期：2020-10-21 00:00:00

abstract：:Haemophilus influenzae type b (Hib) is, in contrast to non-type b H. influenzae, associated with severe invasive disease, such as meningitis and epiglottitis, in small children. To date, accurate H. influenzae capsule typing requires PCR, a time-consuming and cumbersome method. Matrix-assisted laser desorption ionizat...

journal_title：Journal of clinical microbiology

authors： Månsson V,Resman F,Kostrzewa M,Nilson B,Riesbeck K

更新日期：2015-07-01 00:00:00

abstract：:We isolated Shiga toxin-producing Escherichia coli O157:H7 strains resistant to third-generation cephalosporins. The resistant strains harbored blaCMY-2, a plasmid-mediated AmpC β-lactamase. Genotyping of isolates revealed the possible spread of this problematic bacterium. Results suggested the importance of the inves...

journal_title：Journal of clinical microbiology

authors： Kawahara R,Seto K,Taguchi M,Nakajima C,Kumeda Y,Suzuki Y

更新日期：2015-09-01 00:00:00

abstract：:Real-time isothermal nucleic acid sequence-based amplification (RT-NASBA) was applied to the detection of Mycoplasma pneumoniae. In vitro-generated M. pneumoniae RNA was used to assess the sensitivity of the assay. The 95% hit rate was 148 molecules of M. pneumoniae RNA in the amplification and 10(4) molecules of in v...

journal_title：Journal of clinical microbiology

authors： Loens K,Ieven M,Ursi D,Beck T,Overdijk M,Sillekens P,Goossens H

更新日期：2003-09-01 00:00:00

abstract：:The Tigecycline Evaluation and Surveillance Trial (TEST) was designed to monitor global longitudinal changes in bacterial susceptibility to a panel of antimicrobial agents, including tigecycline. In this study, we examine susceptibility among Gram-positive isolates collected from pediatric patients globally between 20...

journal_title：Journal of clinical microbiology

authors： Brandon M,Dowzicky MJ

更新日期：2013-07-01 00:00:00

abstract：:Quantitative PCR (qPCR) is more sensitive than microscopy for detecting Pneumocystis jirovecii in bronchoalveolar lavage (BAL) fluid. We therefore developed a qPCR assay and compared the results with those of a routine immunofluorescence assay (IFA) and clinical data. The assay included automated DNA extraction, ampli...

journal_title：Journal of clinical microbiology

authors： Botterel F,Cabaret O,Foulet F,Cordonnier C,Costa JM,Bretagne S

更新日期：2012-02-01 00:00:00

abstract：:The ability of fluorescence resonance energy transfer, molecular-beacon, and TaqMan probes to detect single nucleotide polymorphism (SNP) in the presence of a wild-type allele was evaluated using drug resistance-conferring SNPs in mixed Mycobacterium tuberculosis DNA. It was found that both the absolute quantity and t...

journal_title：Journal of clinical microbiology

authors： Yesilkaya H,Meacci F,Niemann S,Hillemann D,Rüsch-Gerdes S,LONG DRUG Study Group.,Barer MR,Andrew PW,Oggioni MR

更新日期：2006-10-01 00:00:00

abstract：:The AutoMicrobic system (AMS; Vitek Systems, Inc., Hazelwood, Mo.) was studied for its ability to produce accurate and precise MIC interpretations for Pseudomonas aeruginosa susceptibility to gentamicin, tobramycin, and amikacin. MICs were determined in parallel on 200 selected P. aeruginosa isolates by using the AMS ...

journal_title：Journal of clinical microbiology

authors： Woolfrey BF,Lally RT,Ederer MN,Quall CO

更新日期：1984-04-01 00:00:00

abstract：:A severe outbreak of hemorrhagic colitis occurred in London, Ontario, during the month of September 1985. A total of 55 residents and 18 employees of a nursing home developed diarrhea, and 17 residents (age range, 78 to 99 years) died. Specimens from 38 patients, 37 employees and contacts, and 10 autopsies were invest...

journal_title：Journal of clinical microbiology

authors： Krishnan C,Fitzgerald VA,Dakin SJ,Behme RJ

更新日期：1987-06-01 00:00:00

abstract：:Noroviruses (NVs) are important human pathogens that cause acute gastroenteritis. Genetically related animal enteric NVs have also been described, but there is no evidence of interspecies transmission of NVs. In this study we characterized antibody prevalence among domestic pigs by using recombinant capsid antigens of...

journal_title：Journal of clinical microbiology

authors： Farkas T,Nakajima S,Sugieda M,Deng X,Zhong W,Jiang X

更新日期：2005-02-01 00:00:00

abstract：:When testing 248 clinical isolates of Neisseria gonorrhoeae, the sensitivity was 100% with GonoGen (Becton Dickinson Microbiology Systems), 99.6% (247 of 248) with GonoGen II (Becton Dickinson), 97.2% (241 of 248) with the MicroTrak direct fluorescent-antibody test (Syva), and 97.6% (242 of 248) with Rapid Fermentatio...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Orwig LK

更新日期：1995-02-01 00:00:00