Proposed changes in interpretive criteria and potency of ampicillin and ampicillin-sulbactam disks for susceptibility tests.

abstract：:Forty human clinical Mycobacterium avium-M. intracellulare complex strains isolated in Greece were characterized to the species level by PCR with three sets of primers specific for one or both species. M. avium predominated in both human immunodeficiency virus-positive and -negative patients, but the frequency of M. i...

journal_title：Journal of clinical microbiology

authors： Kyriakopoulos AM,Tassios PT,Matsiota-Bernard P,Marinis E,Tsaousidou S,Legakis NJ

更新日期：1997-11-01 00:00:00

abstract：:A total of 309 Helicobacter pylori isolates from 18 different countries were analyzed with a previously developed lectin typing system. The system was developed by using a proteolytic pretreatment to enhance the carbohydrate fraction of the sample. Four lectins from Ulex europaeus, Lotus tetragonolobus, Erythrina cris...

journal_title：Journal of clinical microbiology

authors： Hynes SO,Broutet N,Wadström T,Mikelsaar M,O'Toole PW,Telford J,Engstrand L,Kamiya S,Mentis AF,Moran AP,Eurohepygast Study Group.

更新日期：2002-01-01 00:00:00

abstract：:Antiserum specific for Salmonella O3 antigen was raised by immunization of rabbits with an artificial glycoconjugate consisting of the synthetic trisaccharide beta-D- Manp (1----4)-alpha-L- Rhap (1----3)-alpha-D-Galp covalently linked to bovine serum albumin (beta- MRG -BSA). Enzyme immunoassays showed that only lipop...

journal_title：Journal of clinical microbiology

authors： Ekwall E,Norberg T,Svenson SB,Lindberg AA

更新日期：1984-05-01 00:00:00

abstract：:Broad-range amplification of bacterial DNA from clinical specimens has proved useful for the diagnosis of various bacterial infections, especially during antimicrobial treatment of the patient. Optimal sample processing protocols for diagnostic broad-range bacterial PCR should release DNA from an array of target organ...

journal_title：Journal of clinical microbiology

authors： Rantakokko-Jalava K,Jalava J

更新日期：2002-11-01 00:00:00

abstract：:The increased prevalence and the severity of oropharyngeal candidiasis in human immunodeficiency virus (HIV)-positive patients are attributed exclusively to the virus-induced immune deficiency of the host. The present study was aimed at answering the question of whether Candida albicans secretory proteinase, a putativ...

journal_title：Journal of clinical microbiology

authors： Ollert MW,Wende C,Görlich M,McMullan-Vogel CG,Borg-von Zepelin M,Vogel CW,Korting HC

更新日期：1995-10-01 00:00:00

abstract：:We recently tested serum from a human immunodeficiency virus-infected patient for the presence of cryptococcal antigen using the Meridian latex agglutination (LA) test (Cryptococcal Antigen Latex Agglutination System). Two pronase-treated serum specimens from the patient had LA titers of 80 and 160, but the patient ha...

journal_title：Journal of clinical microbiology

authors： Whittier S,Hopfer RL,Gilligan P

更新日期：1994-09-01 00:00:00

abstract：:Simple sample-processing methods for PCR detection of Porphyromonas gingivalis, a major pathogen causing adult periodontitis, from saliva were studied. The ability to detect P. gingivalis from 118 salivary samples by PCR after boiling and Chelex 100 processing was compared with bacterial culture. P. gingivalis was det...

journal_title：Journal of clinical microbiology

authors： Mättö J,Saarela M,Alaluusua S,Oja V,Jousimies-Somer H,Asikainen S

更新日期：1998-01-01 00:00:00

abstract：:Staphylococcus aureus ST45 is a major global MRSA lineage with huge strain diversity and a high clinical impact. It is one of the most prevalent carrier lineages but also frequently causes severe invasive disease, such as bacteremia. Little is known about its evolutionary history. In this study, we used whole-genome s...

journal_title：Journal of clinical microbiology

authors： Effelsberg N,Stegger M,Peitzmann L,Altinok O,Coombs GW,Pichon B,Kearns A,Randad PR,Heaney CD,Bletz S,Schaumburg F,Mellmann A

更新日期：2020-12-17 00:00:00

abstract：:Concentrations of Mycobacterium avium-M. intracellulare ranging from 10(-1) to 10(3) CFU/ml were added to blood, placed in Isolator tubes, and held at room temperature for intervals ranging from 4 h to 56 days before being processed (centrifugation and culture on Middlebrook 7H10 agar). At all concentrations tested, M...

journal_title：Journal of clinical microbiology

authors： von Reyn CF,Hennigan S,Niemczyk S,Jacobs NJ

更新日期：1991-06-01 00:00:00

abstract：:The moderately repetitive sequence Ca3 was used to fingerprint strains of Candida albicans isolated from vulvovaginal infections of 10 women and strains isolated from their male partners. The Dendron software package was then used to compare the DNA fingerprints of these strains with those of vaginal commensals from w...

journal_title：Journal of clinical microbiology

authors： Schmid J,Rotman M,Reed B,Pierson CL,Soll DR

更新日期：1993-01-01 00:00:00

abstract：:High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on ty...

journal_title：Journal of clinical microbiology

authors： Castellanos E,Aranaz A,De Buck J

更新日期：2010-04-01 00:00:00

abstract：:An examination of 23 vancomycin-resistant, streptococcuslike isolates of clinical origin revealed a group of 14 to be related to Leuconostoc lactis (type strain, CIP 102422) on the basis of chemotaxonomic studies. These isolates were initially shown to be atypical by classical biochemical tests. However, they were cha...

journal_title：Journal of clinical microbiology

authors： Barreau C,Wagener G

更新日期：1990-08-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAEC) is an emerging enteric pathogen that causes acute and chronic diarrhea among children, human immunodeficiency virus-infected patients, and travelers to developing regions of the world. The pathogenesis of EAEC strains involves the production of biofilm. In this study, we deter...

journal_title：Journal of clinical microbiology

authors： Mohamed JA,Huang DB,Jiang ZD,DuPont HL,Nataro JP,Belkind-Gerson J,Okhuysen PC

更新日期：2007-01-01 00:00:00

abstract：:Mycobacterium tuberculosis infection and nontuberculous mycobacteria (NTM) infections exhibit similar clinical symptoms; however, the therapies for these two types of infections are different. Therefore, the rapid and accurate identification of M. tuberculosis and NTM species is very important for the control of tuber...

journal_title：Journal of clinical microbiology

authors： Xiao G,He X,Zhang S,Liu Y,Liang Z,Liu H,Zhang J,Ou M,Cai S,Lai W,Zhang T,Ren L,Zhang G

更新日期：2020-01-28 00:00:00

abstract：:We examined the reliability and accuracy of a colorimetric assay using Alamar Blue reagent in the performance of susceptibility tests for Candida albicans. We compared the broth macrodilution method recommended by the National Committee for Clinical Laboratory Standards (NCCLS) with a macrodilution method modified wit...

journal_title：Journal of clinical microbiology

authors： Tiballi RN,He X,Zarins LT,Revankar SG,Kauffman CA

更新日期：1995-04-01 00:00:00

abstract：:D-Arabinitol (DA) is a useful diagnostic marker for candidiasis in patients with neutropenia and other high-risk groups, but its use in unselected patients with a broad range of underlying diseases and conditions has not been studied. We used an automated enzymatic fluorometric assay to measure serum DA/creatinine rat...

journal_title：Journal of clinical microbiology

authors： Yeo SF,Huie S,Sofair AN,Campbell S,Durante A,Wong B

更新日期：2006-11-01 00:00:00

abstract：:During a surveillance program associated with the Italian clinical trial for the evaluation of new acellular pertussis vaccines, two bacterial isolates were obtained in cultures of samples from immunocompetent infants who had episodes of cough. Both clinical isolates were identified as Bordetella bronchiseptica by bio...

journal_title：Journal of clinical microbiology

authors： Stefanelli P,Mastrantonio P,Hausman SZ,Giuliano M,Burns DL

更新日期：1997-06-01 00:00:00

abstract：:A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of specific primers. Th...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Tsunomori Y,Seki R

更新日期：2003-11-01 00:00:00

abstract：:We studied the use of dried spots of bodily fluids (plasma, whole blood, and mother's milk) on filter paper as a means of sample collection and storage for human immunodeficiency virus type 1 (HIV-1) viral load testing under stringent field conditions. Plasma placed directly in lysis buffer, which is customarily used ...

journal_title：Journal of clinical microbiology

authors： Ayele W,Schuurman R,Messele T,Dorigo-Zetsma W,Mengistu Y,Goudsmit J,Paxton WA,de Baar MP,Pollakis G

更新日期：2007-03-01 00:00:00

abstract：:The hexagonal surface structure believed to be characteristic for influenza C virus was not initially observed for a newly isolated virus of this type. After a few additional egg passages of the virus, however, the hexagonal structure could be readily seen on many virions. ...

journal_title：Journal of clinical microbiology

authors： Martin ML,Palmer EL,Kendal AP

更新日期：1977-07-01 00:00:00

abstract：:The zygomycetous mold Cokeromyces recurvatus was isolated from the pleural and peritoneal fluids of a 64-year-old man with a history of peptic ulcer disease and alcohol abuse. The patient presented to the hospital with severe abdominal pain and a ruptured duodenal ulcer. This is one of the few times that C.recurvatus ...

journal_title：Journal of clinical microbiology

authors： Munipalli B,Rinaldi MG,Greenberg SB

更新日期：1996-10-01 00:00:00

abstract：:We compared serial measurements of antibodies to mannan and to a cytoplasmic antigen (enzyme-linked immunosorbent assays), detection of mannan and an unidentified candidal antigen (latex agglutination), and assays of mannose and arabinitol (gas chromatographic assay of per-O-acetylated aldonitrile derivatives). In a h...

journal_title：Journal of clinical microbiology

authors： Greenfield RA,Troutt DL,Rickard RC,Altmiller DH

更新日期：1988-03-01 00:00:00

abstract：:Examination of a number of previously published aligned Borrelia 16S rRNA sequences revealed the presence of regions which could serve as oligonucleotide probe targets for both species-specific identification of Borrelia burgdorferi and distinction between genomic groups. Total cellular RNA isolated from Borrelia cult...

journal_title：Journal of clinical microbiology

authors： Marconi RT,Lubke L,Hauglum W,Garon CF

更新日期：1992-03-01 00:00:00

abstract：:Stool microscopy as performed in clinical parasitology laboratories is a complex procedure with subjective interpretation. Quality assurance (QA) programs often emphasize proficiency testing as an assessment tool. We describe a result reproducibility assessment tool, which can form part of a broader QA program, and wh...

journal_title：Journal of clinical microbiology

authors： Libman MD,Gyorkos TW,Kokoskin E,Maclean JD

更新日期：2008-07-01 00:00:00

abstract：:We examined the blastogenic responses of lymphocytes to Staphylococcus aureus antigen in 49 uninfected non-insulin-dependent diabetic subjects and in 56 uninfected nondiabetic subjects. We found a significantly decreased response to this antigen in the diabetic patients (P less than 0.05). Diabetics whose blood sugar ...

journal_title：Journal of clinical microbiology

authors： Casey J,Sturm C Jr

更新日期：1982-01-01 00:00:00

abstract：:A total of 221 isolates of multidrug-resistant Salmonella enterica serovar Typhimurium in Japan were characterized in the present study. The results revealed that clonal serovar Typhimurium definitive phage type 104 strains prevailed and that these strains had drug resistance patterns, integron types, and pulsed-field...

journal_title：Journal of clinical microbiology

authors： Izumiya H,Terajima J,Matsushita S,Tamura K,Watanabe H

更新日期：2001-07-01 00:00:00

abstract：:A slight modification of the chemically defined medium of McVeigh and Morton resulted in an excellent substratum for the cultivation of Paracoccidioides brasiliensis yeast phase. ...

journal_title：Journal of clinical microbiology

authors： Restrepo A,Jiménez BE

更新日期：1980-08-01 00:00:00

abstract：:The sensitivity and specificity of the human immunodeficiency virus (HIV) type 1-specific immunoglobulin G capture enzyme-linked immunosorbent assay (BED-CEIA) were compared with those of the avidity index method to identify recent HIV infection using a panel of 148 samples (81 patients) representing durations of infe...

journal_title：Journal of clinical microbiology

authors： Loschen S,Bätzing-Feigenbaum J,Poggensee G,Cordes C,Hintsche B,Rausch M,Dupke S,Gohlke-Micknis S,Rödig J,Hamouda O,Kücherer C

更新日期：2008-01-01 00:00:00

abstract：:The low incidence of group B streptococcal (GBS) invasive neonatal disease in Mexico has been attributed to the low prevalence of serotype III strains, a major serotype in developed countries. In addition, nontypeable strains account for 12% of the isolates in Mexico and < 1% of the isolates in the United States. In t...

journal_title：Journal of clinical microbiology

authors： Palacios GC,Eskew EK,Solorzano F,Mattingly SJ

更新日期：1997-11-01 00:00:00

abstract：:Rotaviruses were detected by counterimmunoelectro-osmophoresis in the feces of 16 (13.8%) of 116 infants and young children with gastroenteritis during a 5-month period (September 1979 through January 1980) in Ife, Nigeria. The rate of rotavirus detection varied inversely with relative humidity and was highest in Dece...

journal_title：Journal of clinical microbiology

authors： Paul MO,Erinle EA

更新日期：1982-02-01 00:00:00