Genetic similarity of Candida albicans strains from vaginitis patients and their partners.

abstract：:Sapporo-like viruses (SLVs) are associated with acute gastroenteritis in humans. Due to a limited supply of available reagents for diagnosis, little is known about the incidence and pathogenicity of these viruses. We have developed a first-generation generic reverse transcriptase (RT) PCR assay based on a single prime...

journal_title：Journal of clinical microbiology

authors： Vinjé J,Deijl H,van der Heide R,Lewis D,Hedlund KO,Svensson L,Koopmans MP

更新日期：2000-02-01 00:00:00

abstract：:The API Staph-Ident system was evaluated as a means for identifying the species of bovine strains of staphylococci routinely isolated from quarter-milk samples. The species identity of 314 of 581 (54%) isolates of staphylococci was correctly determined by this method. The API Staph-Ident system was more accurate in co...

journal_title：Journal of clinical microbiology

authors： Langlois BE,Harmon RJ,Akers K

更新日期：1983-11-01 00:00:00

abstract：:We describe the serum immunoglobulin A (IgA) antibody response to Norwalk virus infection in human volunteers and compare it with previously described IgM and total antibody responses. Whereas specific IgA and IgM peak within 2 weeks after onset of symptoms, titers of total blocking antibody continue to rise, implying...

journal_title：Journal of clinical microbiology

authors： Erdman DD,Gary GW,Anderson LJ

更新日期：1989-06-01 00:00:00

abstract：:The increased prevalence and the severity of oropharyngeal candidiasis in human immunodeficiency virus (HIV)-positive patients are attributed exclusively to the virus-induced immune deficiency of the host. The present study was aimed at answering the question of whether Candida albicans secretory proteinase, a putativ...

journal_title：Journal of clinical microbiology

authors： Ollert MW,Wende C,Görlich M,McMullan-Vogel CG,Borg-von Zepelin M,Vogel CW,Korting HC

更新日期：1995-10-01 00:00:00

abstract：:Comparative evaluations of immune status for rubella virus are described for enzyme-linked immunosorbent assay, hemagglutination inhibition, and indirect hemagglutination. A 92.1% agreement between enzyme-linked immunosorbent assay and indirect hemagglutination assay was demonstrated for rubella immune status. Enzyme-...

journal_title：Journal of clinical microbiology

authors： Truant AL,Barksdale BL,Huber TW,Elliott LB

更新日期：1983-01-01 00:00:00

abstract：:Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization...

journal_title：Journal of clinical microbiology

authors： Kusunoki S,Ezaki T,Tamesada M,Hatanaka Y,Asano K,Hashimoto Y,Yabuuchi E

更新日期：1991-08-01 00:00:00

abstract：:Serum hepatitis B virus (HBV) DNA was extracted from a chronically infected patient with cocirculation of hepatitis B surface antigen (HBsAg) and anti-HBs antibodies. Direct PCR and clone-derived sequences of the S and overlapped P genes were obtained. DNA sequences and phylogenetic analysis ascribed this isolate to g...

journal_title：Journal of clinical microbiology

authors： Cuestas ML,Mathet VL,Ruiz V,Minassian ML,Rivero C,Sala A,Corach D,Alessio A,Pozzati M,Frider B,Oubiña JR

更新日期：2006-06-01 00:00:00

abstract：:We have developed an easy and rapid detection and identification system for the diagnosis of mycobacterial diseases. The system is based on selective amplification by PCR of mycobacteria with primers based on the genes coding for 16S rRNA. During PCR, a label (digoxigenin-11-dUTP) is incorporated with biotinylated spe...

journal_title：Journal of clinical microbiology

authors： De Beenhouwer H,Liang Z,De Rijk P,Van Eekeren C,Portaels F

更新日期：1995-11-01 00:00:00

abstract：:Members of the genus Abiotrophia, formerly known as nutritionally variant streptococci, are important pathogens causing septicemia and endocarditis. Cultivation and biochemical differentiation of Abiotrophia spp. are often difficult. Based on 16S rRNA sequences, two PCR assays for detection and identification of Abiot...

journal_title：Journal of clinical microbiology

authors： Roggenkamp A,Leitritz L,Baus K,Falsen E,Heesemann J

更新日期：1998-10-01 00:00:00

abstract：:A 44-year-old diabetic female presented to a hospital in Jamaica with thermal burns. Trichosporon asahii was isolated from facial wounds, sputum, and a meningeal swab. Dissemination of the fungus was demonstrated in stained histological sections of the meninges and a brain abscess at autopsy. Pure growth of the fungus...

journal_title：Journal of clinical microbiology

authors： Heslop OD,Nyi Nyi MP,Abbott SP,Rainford LE,Castle DM,Coard KC

更新日期：2011-12-01 00:00:00

abstract：:This paper describes the first isolation of a new Shigella flexneri serotype, designated 4s, in Beijing, China. Genotypic and phenotypic profiling suggests that this isolate is a clone of the S. flexneri serotype X variant reference strain. Of particular concern is the multidrug resistance exhibited by this isolate. ...

journal_title：Journal of clinical microbiology

authors： Qiu S,Wang Z,Chen C,Liu N,Jia L,Liu W,Wang L,Hao R,Zhang L,Wang Y,Song H

更新日期：2011-03-01 00:00:00

abstract：:Actinobacillus pleuropneumoniae strains of serotypes 1 and 9 were studied for their serological properties by means of agglutination, coagglutination (CoA), indirect hemagglutination (IHA), Co-IHA, ring precipitation (RP), and immunodiffusion (ID) tests. Particulate and soluble antigens of unheated and heat-treated ba...

journal_title：Journal of clinical microbiology

authors： Mittal KR

更新日期：1990-03-01 00:00:00

abstract：:The objective of this hospital-based study was to determine the relationship between colonizing and infecting strains of Candida species and Torulopsis glabrata. Surveillance cultures from high-risk patients were paired with subsequent bloodstream isolates. Organisms were typed by using restriction endonuclease digest...

journal_title：Journal of clinical microbiology

authors： Reagan DR,Pfaller MA,Hollis RJ,Wenzel RP

更新日期：1990-12-01 00:00:00

abstract：:The objective of this study was to measure the performance of the Affirm Ambient Temperature Transport System (ATTS) over time and to estimate the length of time the system can preserve a vaginal specimen containing the three common organisms causing vaginitis: Trichomonas vaginalis, Candida species, and Gardnerella v...

journal_title：Journal of clinical microbiology

authors： Brown HL,Fuller DA,Davis TE,Schwebke JR,Hillier SL

更新日期：2001-09-01 00:00:00

abstract：:Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Siqueira LPM,Gimenes VMF,de Freitas RS,Melhem MSC,Bonfietti LX,da Silva AR Jr,Souza Santos LB,Motta AL,Rossi F,Benard G,de Almeida JN Jr

更新日期：2019-01-02 00:00:00

abstract：:Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuc...

journal_title：Journal of clinical microbiology

authors： Conville PS,Fischer SH,Cartwright CP,Witebsky FG

更新日期：2000-01-01 00:00:00

abstract：:Osiris is a video zone size reader for disk diffusion tests featuring a built-in extended expert system (EES). The efficacy of the EES for the identification of the beta-lactam susceptibility phenotypes of Pseudomonas aeruginosa isolates was evaluated. Thirteen beta-lactams were tested in four laboratories by the disk...

journal_title：Journal of clinical microbiology

authors： Bert F,Ould-Hocine Z,Juvin M,Dubois V,Loncle-Provot V,Lefranc V,Quentin C,Lambert N,Arlet G

更新日期：2003-08-01 00:00:00

abstract：:Counterimmunoelectrophoresis was compared with electron microscopy for detection of human reovirus-like agent in fecal specimens. Both tests gave very similar results. ...

journal_title：Journal of clinical microbiology

authors： Spence L,Fauvel M,Petro R,Bloch S

更新日期：1977-02-01 00:00:00

abstract：:A total of 141 independent strains of Pseudomonas aeruginosa with different heterogeneities in the exo gene (exoS, exoT, exoU, and exoY) background were examined for their pathogenic roles. Results indicated that the exoU gene is the major contributor to cytotoxicity in Madin-Darby canine kidney cells but is not relat...

journal_title：Journal of clinical microbiology

authors： Lin HH,Huang SP,Teng HC,Ji DD,Chen YS,Chen YL

更新日期：2006-12-01 00:00:00

abstract：:We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The d...

journal_title：Journal of clinical microbiology

authors： Korbsrisate S,Suwanasai N,Leelaporn A,Ezaki T,Kawamura Y,Sarasombath S

更新日期：1999-11-01 00:00:00

abstract：:A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg. ...

journal_title：Journal of clinical microbiology

authors： Zhao Y,Petraitiene R,Walsh TJ,Perlin DS

更新日期：2013-03-01 00:00:00

abstract：:The impact of the viability of Trichomonas vaginalis in urine on wet mount, culture, and PCR methods was assessed. To minimize the chance of false-negative results, urine specimens should be processed within 30 min of specimen collection and maintained at 37 degrees C, since temperature appears to affect the viability...

journal_title：Journal of clinical microbiology

authors： Shafir SC,Sorvillo FJ

更新日期：2006-10-01 00:00:00

abstract：:The effects of Tween 80 supplementation of liquid culture medium on the formation of the superficial L1 layer of the Mycobacterium avium-Mycobacterium intracellulare complex (MAC) were examined by serological and scanning electron microscopic experiments. Specific antiserum to the glycopeptidolipids on the L1 layer of...

journal_title：Journal of clinical microbiology

authors： Masaki S,Sugimori G,Okamoto A,Imose J,Hayashi Y

更新日期：1991-07-01 00:00:00

abstract：:Gemella morbillorum and Gemella haemolysans are opportunistic pathogens which cause endocarditis and other severe infections. We report on three patients with endocarditis, one with endocarditis caused by G. haemolysans and two with endocarditis caused by G. morbillorum. The paucity of reports concerning these bacteri...

journal_title：Journal of clinical microbiology

authors： La Scola B,Raoult D

更新日期：1998-04-01 00:00:00

abstract：:Four slightly yellow-pigmented, alpha-hemolytic, gram-negative coccobacilli, three from wound specimens and one from multiple blood cultures of a patient with endocarditis, were identified as Neisseria elongata subsp. glycolytica on the basis of their overall biochemical and genetic similarities to this subspecies. Th...

journal_title：Journal of clinical microbiology

authors： Andersen BM,Weyant RS,Steigerwalt AG,Moss CW,Hollis DG,Weaver RE,Ashford D,Brenner DJ

更新日期：1995-01-01 00:00:00

abstract：:The stability of cervical specimens in SurePath preservative fluid for human papillomavirus (HPV) testing with Roche cobas 4800 was determined using a panel of 308 pooled specimens from a colposcopy referral population. The SurePath specimens appeared to be stable for up to 10 weeks at ambient temperature for HPV test...

journal_title：Journal of clinical microbiology

authors： Gilbert L,Oates E,Ratnam S

更新日期：2013-10-01 00:00:00

abstract：:Trauma intensive care unit (TICU) patients requiring mechanical respiratory support frequently develop ventilator-associated pneumonia (VAP). Oral and oropharyngeal bacteria are believed to be responsible for many cases of VAP, but definitive evidence of this relationship is lacking. Earlier studies used conventional ...

journal_title：Journal of clinical microbiology

authors： Bahrani-Mougeot FK,Paster BJ,Coleman S,Barbuto S,Brennan MT,Noll J,Kennedy T,Fox PC,Lockhart PB

更新日期：2007-05-01 00:00:00

abstract：:In Brazil, the most common etiological agent of American tegumentary leishmaniasis is Leishmania (Viannia) braziliensis. In general, diagnostic techniques envisage the visualization of the parasite, but that technique has a low sensitivity. The main purpose of the present work was to evaluate the PCR as a routine tool...

journal_title：Journal of clinical microbiology

authors： Pirmez C,da Silva Trajano V,Paes-Oliveira Neto M,da-Cruz AM,Gonçalves-da-Costa SC,Catanho M,Degrave W,Fernandes O

更新日期：1999-06-01 00:00:00

abstract：:Comparative sequence analysis was performed upon Bacillus anthracis and its closest relatives, B. cereus and B. thuringiensis. Portions of rpoB DNA from 10 strains of B. anthracis, 16 of B. cereus, 10 of B. thuringiensis, 1 of B. mycoides, and 1 of B. megaterium were amplified and sequenced. The determined rpoB sequen...

journal_title：Journal of clinical microbiology

authors： Ko KS,Kim JM,Kim JW,Jung BY,Kim W,Kim IJ,Kook YH

更新日期：2003-07-01 00:00:00

abstract：:The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) has infected at least 1,082 people, including 439 fatalities. So far, no empirical virus isolation study has been done to elucidate infectious virus secretion or serotype variability. Here, we used 51 respiratory samples from 32 patients with co...

journal_title：Journal of clinical microbiology

authors： Muth D,Corman VM,Meyer B,Assiri A,Al-Masri M,Farah M,Steinhagen K,Lattwein E,Al-Tawfiq JA,Albarrak A,Müller MA,Drosten C,Memish ZA

更新日期：2015-09-01 00:00:00