Characterization of Leuconostoc lactis strains from human sources.

abstract：:The CLSI (formerly NCCLS) M38-A document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. A multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (MICs and m...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Fothergill A,Ghannoum M,Manavathu E,Ostrosky-Zeichner L,Pfaller MA,Rinaldi MG,Schell W,Walsh TJ

更新日期：2007-07-01 00:00:00

abstract：:The conventional phage typing scheme proposed by S. Basu and S. Mukerjee (Experientia 24:299-300, 1968) has been used routinely for identification of the strains at the Vibrio Phage Reference Laboratory since 1968. However, because of limitations of this scheme, a new phage typing scheme using five newly isolated phag...

journal_title：Journal of clinical microbiology

authors： Chattopadhyay DJ,Sarkar BL,Ansari MQ,Chakrabarti BK,Roy MK,Ghosh AN,Pal SC

更新日期：1993-06-01 00:00:00

abstract：:An enzyme-linked immunosorbent microassay using nitrocellulose paper as the solid-phase support was developed for the detection of peste-des-petits-ruminants virus antigens in infected caprine tissue homogenates. Dots of tissue homogenates were applied to nitrocellulose papers, and any unreacted sites were blocked wit...

journal_title：Journal of clinical microbiology

authors： Obi TU,Ojeh CK

更新日期：1989-09-01 00:00:00

abstract：:The Lyme disease-associated spirochete, Borrelia burgdorferi, is maintained in enzootic cycles involving Ixodes ticks and small mammals. Previous studies demonstrated that B. burgdorferi expresses outer surface protein A (OspA) but not OspC when residing in the midgut of unfed ticks. However, after ticks feed on blood...

journal_title：Journal of clinical microbiology

authors： Schwan TG,Piesman J

更新日期：2000-01-01 00:00:00

abstract：:A new liquid medium (modified Lombard-Dowell broth) was inoculated with stock culture strains of aerobic and anaerobic bacteria and compared with prereduced chopped-meat glucose inoculated with the same anaerobes. Both broths were subcultured at 48 and 72 h to aerobic and anaerobic blood agar plates, and the numbers o...

journal_title：Journal of clinical microbiology

authors： Jessee MT,Robinson PJ

更新日期：1977-11-01 00:00:00

abstract：:The slightly revised, current scheme of 20 flagellar (H) antigens of Serratia marcesens was examined. The seven new H antigens were demonstrated to be antigenically distinct as determined with Le Minor's H-immobilization test. The H-immobilization antibodies of rabbit anti-H immune sera proved resistant to treatment w...

journal_title：Journal of clinical microbiology

authors： Traub WH,Fukushima PI

更新日期：1979-07-01 00:00:00

abstract：:We compared the Abbott enzyme immunoassay for human immunodeficiency virus (HIV) antigen with the reverse transcriptase assay (RTA) as a means of monitoring HIV infection during an antiviral trial. The Abbott enzyme immunoassay detected HIV earlier than RTA whether or not the patients were antigenemic and appears to b...

journal_title：Journal of clinical microbiology

authors： Burger H,Paul D,Siegal FP,Wendel I,Neff S,Eilbott D,Gehan K,Grimson R,Weiser B

更新日期：1988-09-01 00:00:00

abstract：:Streptococcus pyogenes, a major human pathogen, is still considered susceptible to beta-lactams, but for other relevant antibiotics, highly variable resistance rates have been reported. Since no data were available from Iran, we tested 1,335 throat isolates from two different regions of the country for their antibioti...

journal_title：Journal of clinical microbiology

authors： Jasir A,Tanna A,Noorani A,Mirsalehian A,Efstratiou A,Schalen C

更新日期：2000-06-01 00:00:00

abstract：:Traveler's diarrhea (TD) is the most common infectious illness acquired by visitors to developing nations. The purpose of this study was to utilize molecular diagnostic techniques to determine the prevalence of norovirus (NoV) in TD occurring among visitors from the United States to Guatemala and Mexico. Stool samples...

journal_title：Journal of clinical microbiology

authors： Chapin AR,Carpenter CM,Dudley WC,Gibson LC,Pratdesaba R,Torres O,Sanchez D,Belkind-Gerson J,Nyquist I,Kärnell A,Gustafsson B,Halpern JL,Bourgeois AL,Schwab KJ

更新日期：2005-03-01 00:00:00

abstract：:Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97 (95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399). ...

journal_title：Journal of clinical microbiology

authors： Twin J,Taylor N,Garland SM,Hocking JS,Walker J,Bradshaw CS,Fairley CK,Tabrizi SN

更新日期：2011-03-01 00:00:00

abstract：:Restriction endonucleases BamHI, BclI, BglI, BglII, BstEII, EcoRI, HindIII, HpaI, SalI, SmalI, XbalI, and XholI were used to analyze 61 selected strains of adenovirus type 3 (Ad3) isolated from Africa, Asia, Australia, Europe, North America, and South America. It was noted that the use of BamHI, BclI, BglII, HpaI, Sal...

journal_title：Journal of clinical microbiology

authors： Li QG,Wadell G

更新日期：1988-05-01 00:00:00

abstract：:The clinical value of immunoenzymatic (enzyme-linked immunosorbent assay) detection of anti-Toxoplasma immunoglobulin E (IgE) was assessed by studying 2,036 sera from 792 subjects, comprising seronegative controls and subjects with acute, active, reactivated, or congenital toxoplasmosis. Included were nonimmunized adu...

journal_title：Journal of clinical microbiology

authors： Foudrinier F,Villena I,Jaussaud R,Aubert D,Chemla C,Martinot F,Pinon JM

更新日期：2003-04-01 00:00:00

abstract：:Clostridium hathewayi is a newly described Clostridium species isolated from the feces of healthy human individuals, but its clinical significance is not known. We describe a case of human infection associated with C. hathewayi. The bacterium (strain HKU18) was isolated from the blood culture of a 39-year-old patient ...

journal_title：Journal of clinical microbiology

authors： Woo PC,Lau SK,Woo GK,Fung AM,Yiu VP,Yuen KY

更新日期：2004-12-01 00:00:00

abstract：:Carbapenem-resistant Enterobacter species are emerging nosocomial pathogens. As with most multidrug-resistant Gram-negative pathogens, the polymyxins are often the only therapeutic option. In this study involving clinical isolates of E. cloacae and E. aerogenes, susceptibility testing methods with polymyxin B were ana...

journal_title：Journal of clinical microbiology

authors： Landman D,Salamera J,Quale J

更新日期：2013-12-01 00:00:00

abstract：:We describe the isolation of Actinobacillus lignieresii and an A. equuli-like bacterium from an infected horse-bite wound in a 22-year-old stable foreman and A. suis from a bite injury in a 35-year-old man who had been attacked by a horse. A. lignieresii was also isolated in pure culture from an infected sheep-bite wo...

journal_title：Journal of clinical microbiology

authors： Peel MM,Hornidge KA,Luppino M,Stacpoole AM,Weaver RE

更新日期：1991-11-01 00:00:00

abstract：:Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and ...

journal_title：Journal of clinical microbiology

authors： Lebrun L,Espinasse F,Poveda JD,Vincent-Levy-Frebault V

更新日期：1992-09-01 00:00:00

abstract：:Amplified fragment length polymorphism (AFLP) was employed as a genetic analysis tool for the study of the genetic relatedness of Mycobacterium avium subsp. paratuberculosis isolates harvested from bovine fecal samples and from bovine or human tissues. This analysis revealed genetic differences between these two isola...

journal_title：Journal of clinical microbiology

authors： O'Shea B,Khare S,Klein P,Roussel A,Adams LG,Ficht TA,Rice-Ficht AC

更新日期：2011-06-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of human immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by using lipopolysaccharides as antigens is described. The results obtained with the lipopolysaccharide ELISA were compared with the results of the wh...

journal_title：Journal of clinical microbiology

authors： Granfors K,Viljanen MK,Toivanen A

更新日期：1981-07-01 00:00:00

abstract：:Antibody responses of 85 patients to human immunodeficiency virus type 1 antigens were quantitated by densitometric analysis of Western blot (immunoblot) assays. All patients had been classified into the following three clinical categories: asymptomatic (ASY), acquired immunodeficiency syndrome (AIDS)-related complex ...

journal_title：Journal of clinical microbiology

authors： Schmidt G,Amiraian K,Frey H,Wethers J,Stevens RW,Berns DS

更新日期：1989-05-01 00:00:00

abstract：:The prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (ESBLEC) in Spain increased 8-fold from 2000 to 2006. ESBL type, clonal relationship, antimicrobial susceptibility, and clinical data about infections caused by ESBLEC are evaluated in a second nationwide study developed in 2006. From...

journal_title：Journal of clinical microbiology

authors： Díaz MA,Hernández-Bello JR,Rodríguez-Baño J,Martínez-Martínez L,Calvo J,Blanco J,Pascual A,Spanish Group for Nosocomial Infections (GEIH).

更新日期：2010-08-01 00:00:00

abstract：:The fungus Fusarium sacchari was isolated repeatedly from the blood of an immunosuppressed host. The infection was treated successfully with a small dose of amphotericin B. The strain was resistant to this antifungal in vitro. MICs and minimum fungicidal concentrations of six antifungals for the clinical isolate are p...

journal_title：Journal of clinical microbiology

authors： Guarro J,Nucci M,Akiti T,Gené J,Barreiro MD,Gonçalves RT

更新日期：2000-01-01 00:00:00

abstract：:By using primers based on the sequence of a species-specific antigen of Helicobacter pylori (P. O'Toole, S.M. Logan, M. Kostrzynska. T. Wadström, and T.J. Trust, J. Bacteriol. 173:505-513, 1991), a protocol was established for detection of this microorganism in gastric biopsy samples by the polymerase chain reaction (...

journal_title：Journal of clinical microbiology

authors： Hammar M,Tyszkiewicz T,Wadström T,O'Toole PW

更新日期：1992-01-01 00:00:00

abstract：:We compared two multistep diagnostic algorithms based on C. Diff Quik Chek Complete and, as confirmatory tests, GenomEra C. difficile and Xpert C. difficile. The sensitivity, specificity, positive predictive value, and negative predictive value were 87.2%, 99.7%, 97.1%, and 98.3%, respectively, for the GenomEra-based ...

journal_title：Journal of clinical microbiology

authors： Alcalá L,Reigadas E,Marín M,Fernández-Chico A,Catalán P,Bouza E

更新日期：2015-01-01 00:00:00

abstract：:Recent studies have shown the public health importance of identifying individuals with acute human immunodeficiency virus infection (AHI); however, the cost of nucleic acid amplification testing (NAAT) makes individual testing of at-risk individuals prohibitively expensive in many settings. Pooled NAAT (or group testi...

journal_title：Journal of clinical microbiology

authors： Westreich DJ,Hudgens MG,Fiscus SA,Pilcher CD

更新日期：2008-05-01 00:00:00

abstract：:We compared 14 molecular assays for their ability to detect the Mycobacterium tuberculosis complex in bronchoalveolar lavage fluid samples. Three approaches were followed. First, by using DNA from Mycobacterium bovis BCG, we determined the detection limits of the assays using routine molecular methods. Second, in orde...

journal_title：Journal of clinical microbiology

authors： Akkerman OW,van der Werf TS,de Boer M,de Beer JL,Rahim Z,Rossen JW,van Soolingen D,Kerstjens HA,van der Zanden AG

更新日期：2013-11-01 00:00:00

abstract：:The lack of a quick, simple, and inexpensive diagnostic test has limited the ability of public health officials to rapidly assess and control outbreaks of Giardia lamblia in child day-care centers. We evaluated the performance of a commercially available enzyme-linked immunosorbent assay (ELISA) for the detection of a...

journal_title：Journal of clinical microbiology

authors： Addiss DG,Mathews HM,Stewart JM,Wahlquist SP,Williams RM,Finton RJ,Spencer HC,Juranek DD

更新日期：1991-06-01 00:00:00

abstract：:We report the first case of spontaneous intrapartum Atopobium vaginae bacteremia identified by 16S rRNA gene sequencing. The bacterium was misidentified by RapID ANA II, API Rapid ID 32A, and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The likely source of bacteremia wa...

journal_title：Journal of clinical microbiology

authors： Chan JF,Lau SK,Curreem SO,To KK,Leung SS,Cheng VC,Yuen KY,Woo PC

更新日期：2012-07-01 00:00:00

abstract：:The serological properties of six new pneumococcal capsular types are described. A table listing all 90 pneumococcal types and their cross-reactions is included. ...

journal_title：Journal of clinical microbiology

authors： Henrichsen J

更新日期：1995-10-01 00:00:00

abstract：:A sensitive rolling-circle amplification (RCA)-based method utilizing species-specific padlock probes targeted to the internal transcribed spacer 2 region of the fungal ribosomal DNA gene complex was developed. The assay was rapid (2 hours) and specific. Of 28 fungal isolates (16 of Candida, six of Aspergillus, and si...

journal_title：Journal of clinical microbiology

authors： Zhou X,Kong F,Sorrell TC,Wang H,Duan Y,Chen SC

更新日期：2008-07-01 00:00:00

abstract：:Production of staphylococcal alpha- or alpha-beta-toxins correlated well with production of coagulase or thermonuclease (or both) in 203 Staphylococcus aureus isolates from milk and should be reliable indicators of S. aureus in the absence of Staphylococcus intermedius. Failures to produce toxin, tube coagulase, or th...

journal_title：Journal of clinical microbiology

authors： Jasper DE,Infante F,Dellinger JD

更新日期：1985-04-01 00:00:00