Global Epidemiology and Evolutionary History of Staphylococcus aureus ST45.

abstract：:The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with HindIII, EcoRV, and AspI to generate restriction fragmen...

journal_title：Journal of clinical microbiology

authors： Carter SE,Ravyn MD,Xu Y,Johnson RC

更新日期：2001-09-01 00:00:00

abstract：:A group of 300 clinically derived isolates of coagulase-negative staphylococci were tested in parallel with the API STAPH-IDENT system (Analytab Products) and 14 conventional biochemical tests contained in Kloos and Schleifer's simplified scheme for identification of human Staphylococcus species. STAPH-IDENT is a mini...

journal_title：Journal of clinical microbiology

authors： Almeida RJ,Jorgensen JH

更新日期：1983-08-01 00:00:00

abstract：:A multiplex PCR (mPCR) protocol was developed for simultaneous detection of the gyrB gene in Streptococcus pneumoniae, Streptococcus mitis, and Streptococcus oralis, and the specificity was evaluated using 141 coccus strains. Genomic DNAs purified from S. pneumoniae, S. mitis, and S. oralis strains were efficiently de...

journal_title：Journal of clinical microbiology

authors： Kim W,Park HK,Hwang WJ,Shin HS

更新日期：2013-03-01 00:00:00

abstract：:A collection of 54 unrelated Streptococcus agalactiae strains isolated from cerebrospinal fluid samples from neonates and 60 unrelated strains isolated from carriers that had been previously studied by multilocus enzyme electrophoresis (R. Quentin, H. Huet, F.-S. Wang, P. Geslin, A. Goudeau, and R. K. Selander, J. Cli...

journal_title：Journal of clinical microbiology

authors： Chatellier S,Ramanantsoa C,Harriau P,Rolland K,Rosenau A,Quentin R

更新日期：1997-10-01 00:00:00

abstract：:Antibody to equid herpesvirus 1, which mediates single radial hemolysis, is that responsible for neutralization. Hemagglutination inhibition antibody is not necessarily involved in neutralization or hemolysis. ...

journal_title：Journal of clinical microbiology

authors： Klingeborn B,Dinter Z

更新日期：1978-05-01 00:00:00

abstract：:The molecular epidemiology of tuberculosis (TB) in Finland was studied by spoligotyping 380 Mycobacterium tuberculosis isolates. The isolates were obtained during a 1-year study period from July 2000 to June 2001 and represented 90% of new M. tuberculosis findings by culture in the whole country during the study perio...

journal_title：Journal of clinical microbiology

authors： Puustinen K,Marjamäki M,Rastogi N,Sola C,Filliol I,Ruutu P,Holmström P,Viljanen MK,Soini H

更新日期：2003-04-01 00:00:00

abstract：:Monoclonal antibodies were generated from mice immunized with scolex protein antigen of Cysticercus cellulosae. Three monoclonal antibodies specific for cysticercal antigens, which did not show any cross-reactivity with Taenia solium or Taenia saginata antigens, were selected. Each monoclonal antibody coupled to Sepha...

journal_title：Journal of clinical microbiology

authors： Nascimento E,Tavares CA,Lopes JD

更新日期：1987-07-01 00:00:00

abstract：:Two serotypes of exfoliatin, A and B, previously reported by Kondo et al. (1974) were examined for their presence in 43 strains of Staphylococcus aureus, most of which were isolated from patients with Ritter's disease and impetigo. The tested strains consisted of 24 strains of phage group II and 19 strains not of phag...

journal_title：Journal of clinical microbiology

authors： Kondo I,Sakurai S,Sarai Y,Futaki S

更新日期：1975-05-01 00:00:00

abstract：:Feces and serum specimens were collected from three farms in Michigan on which approximately 50-lb (8- to 9-week-old) pigs experienced diarrhea just after placement into all-in-all-out finishing barns. The clinical signs (profuse watery diarrhea lasting about 2 weeks and no vomiting) were similar on all farms, and the...

journal_title：Journal of clinical microbiology

authors： Kim Y,Chang KO,Straw B,Saif LJ

更新日期：1999-05-01 00:00:00

abstract：:The aim of this study was to develop a PCR and reverse line blot hybridization (PCR-RLB) macroarray assay based on 16S-23S rRNA gene internal transcribed spacer sequences for the identification and differentiation of 34 mycobacterial species or subspecies. The performance of the PCR-RLB assay was assessed and validate...

journal_title：Journal of clinical microbiology

authors： Xiong L,Kong F,Yang Y,Cheng J,Gilbert GL

更新日期：2006-10-01 00:00:00

abstract：:The 30-micrograms cefprozil disk and the cephalosporin class (30-micrograms cephalothin) disk were compared for their abilities to predict cefprozil susceptibility by agar disk diffusion testing. High error (5.02% major and 14.11% minor) rates were encountered with the cephalothin disk and were most frequently observe...

journal_title：Journal of clinical microbiology

authors： Fung-Tomc J,Stickle T,Doyle C,Huczko E,Kessler RE

更新日期：1991-11-01 00:00:00

abstract：:Rotavirus is the most common cause of severe dehydrating gastroenteritis in infants. To date, 10 different serotypes of rotavirus have been identified in human stools. While four or five serotypes dominate, serotype circulation varies with season and geography. Since our laboratory has been involved in the development...

journal_title：Journal of clinical microbiology

authors： DiStefano DJ,Kraiouchkine N,Mallette L,Maliga M,Kulnis G,Keller PM,Clark HF,Shaw AR

更新日期：2005-12-01 00:00:00

abstract：:Restriction endonucleases BamHI, BclI, BglI, BglII, BstEII, EcoRI, HindIII, HpaI, SalI, SmalI, XbalI, and XholI were used to analyze 61 selected strains of adenovirus type 3 (Ad3) isolated from Africa, Asia, Australia, Europe, North America, and South America. It was noted that the use of BamHI, BclI, BglII, HpaI, Sal...

journal_title：Journal of clinical microbiology

authors： Li QG,Wadell G

更新日期：1988-05-01 00:00:00

abstract：:AmpC regulatory genes in 21 ceftazidime-resistant clinical isolates of Enterobacter cloacae (MICs of > or = 16 microg/ml) were characterized. All isolates exhibited AmpC overproduction due to AmpD mutation. Additionally, we found two AmpR mutants among the isolates. This is the first report of chromosomal ampR mutatio...

journal_title：Journal of clinical microbiology

authors： Kaneko K,Okamoto R,Nakano R,Kawakami S,Inoue M

更新日期：2005-06-01 00:00:00

abstract：:Prosthetic joint infection (PJI) is a rare but refractory complication of arthroplasty. Accurate identification of pathogens is a key step for successful treatment of PJI, which remains a challenge for clinicians and laboratory workers. We designed a combined culture method with sonication of implants and incubation i...

journal_title：Journal of clinical microbiology

authors： Shen H,Tang J,Wang Q,Jiang Y,Zhang X

更新日期：2015-03-01 00:00:00

abstract：:HIV-1 drug resistance (HIVDR) assays are important tools in clinical management of HIV-infected patients on antiretroviral therapy (ART) and surveillance of drug-resistant variants at population levels. The high cost associated with commercial assays hinders their use in resource-limited settings. We adopted and valid...

journal_title：Journal of clinical microbiology

authors： Inzaule S,Yang C,Kasembeli A,Nafisa L,Okonji J,Oyaro B,Lando R,Mills LA,Laserson K,Thomas T,Nkengasong J,Zeh C

更新日期：2013-02-01 00:00:00

abstract：:The usefulness of pneumococcal urinary antigen tests (UATs) in severe pneumococcal infection relies heavily on the performance in bacteremic patients. Fluorescence technology and automatic reading of test results may improve UAT performance. We evaluated the automatically read Sofia S. pneumoniae FIA for diagnosing pn...

journal_title：Journal of clinical microbiology

authors： Olofsson E,Özenci V,Athlin S

更新日期：2019-07-26 00:00:00

abstract：:High-performance liquid chromatography was evaluated as a rapid means of identifying obligately anaerobic gram-positive cocci of medical interest. Isolates were inoculated into a defined chemical medium consisting primarily of amino acids and were incubated aerobically for 1 h at 35 degrees C. After removal of organis...

journal_title：Journal of clinical microbiology

authors： Harpold DJ,Wasilauskas BL

更新日期：1987-06-01 00:00:00

abstract：:The aim of this study was to determine if gastroenteric viruses were present on surfaces and equipment in a pediatric primary immunodeficiency unit (PPIU) by environmental sampling using swabs and subsequent nucleic acid extraction and reverse transcriptase PCR assays. A PPIU was chosen, and 11 swabs were taken at the...

journal_title：Journal of clinical microbiology

authors： Gallimore CI,Taylor C,Gennery AR,Cant AJ,Galloway A,Iturriza-Gomara M,Gray JJ

更新日期：2006-02-01 00:00:00

abstract：:A recent case report of a child infected with enterohemorrhagic Escherichia coli (EHEC) of serotype O118:H16 in Bavaria, in association with the isolation of a bovine O118 strain on the same farm (A. Weber, H. Klie, H. Richter, P. Gallien, M. Timm, and K. W. Perlberg, Berl. Muench. Tieraerztl. Wochenschr. 110:211-213,...

journal_title：Journal of clinical microbiology

authors： Wieler LH,Busse B,Steinrück H,Beutin L,Weber A,Karch H,Baljer G

更新日期：2000-06-01 00:00:00

abstract：:The pathogenesis of gastroduodenal diseases is related to the diversity of Helicobacter pylori strains. CagA-positive strains are more likely to cause gastric cancer than CagA-negative strains. Based on EPIYA (Glu-Pro-Ile-Tyr-Ala) motifs at the carboxyl terminus corresponding to phosphorylation sites, H. pylori CagA i...

journal_title：Journal of clinical microbiology

authors： Truong BX,Mai VT,Tanaka H,Ly le T,Thong TM,Hai HH,Van Long D,Furumatsu K,Yoshida M,Kutsumi H,Azuma T

更新日期：2009-12-01 00:00:00

abstract：:Herpes simplex virus type 2 (HSV-2) seroprevalence in 68 patients with AIDS was 77% by Western blot (immunoblot) and 44% by glycoprotein G-2 immunoassay. Each of 16 patients with culture-proven HSV-2 infection was positive by Western blot versus 8 by glycoprotein G-2 immunoassay. No differences in age, race, duration ...

journal_title：Journal of clinical microbiology

authors： Safrin S,Arvin A,Mills J,Ashley R

更新日期：1992-05-01 00:00:00

abstract：:Many truly human immunodeficiency virus (HIV) antibody-negative serum samples may be unnecessarily subjected to costly and time-consuming Western blots (immunoblots). An investigation was undertaken to evaluate the efficiency of using a recombinant protein-based enzyme immunosorbent assay (EIA; Cambridge BioScience [C...

journal_title：Journal of clinical microbiology

authors： Lepine DG,Neumann PW,Frenette SL,O'Shaughnessy MV

更新日期：1990-06-01 00:00:00

abstract：:Characteristics differentiating Escherichia coli strains that cause cystitis or pyelonephritis from fecal E. coli remain incompletely defined, particularly among adult women in the United States. Accordingly, phylogenetic group, O antigens, and virulence factors (VFs) were analyzed among 329 E. coli isolates from the ...

journal_title：Journal of clinical microbiology

authors： Johnson JR,Owens K,Gajewski A,Kuskowski MA

更新日期：2005-12-01 00:00:00

abstract：:The aims of this study were to compare a novel PCR-based Chlamydia trachomatis detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available C. trachomatis detection Hybrid Capture 2 (HC2) assay and to investigate the C. trachomatis serovar distribution among young women in a rural Costa Rican s...

journal_title：Journal of clinical microbiology

authors： Quint K,Porras C,Safaeian M,González P,Hildesheim A,Quint W,van Doorn LJ,Silva S,Melchers W,Schiffman M,Rodríguez AC,Wacholder S,Freer E,Cortes B,Herrero R,Costa Rican Vaccine Trial Group.

更新日期：2007-12-01 00:00:00

abstract：:Three strains of a Pasteurella haemolytica-like bacterium were isolated from lesions of a progressive granuloma of cattle in southern Brazil. Their characteristics and their differentiation from P. haemolytica varieties and Actinobacillus lignieresii are described. The name "Pasteurella granulomatis" is proposed for t...

journal_title：Journal of clinical microbiology

authors： Ribeiro GA,Carter GR,Frederiksen W,Riet-Correa F

更新日期：1989-06-01 00:00:00

abstract：:We recently described a novel megaplasmid-encoded adhesin produced by certain Shiga toxigenic Escherichia coli (STEC) strains that lack the locus for enterocyte effacement (LEE) pathogenicity island. This adhesin, designated Saa (STEC autoagglutinating adhesin), may be a marker for a subset of LEE-negative STEC strain...

journal_title：Journal of clinical microbiology

authors： Paton AW,Paton JC

更新日期：2002-01-01 00:00:00

abstract：:The identification of Pasteurella and related bacteria remains a challenge. Here, a 449- to 473-bp fragment (sodA(int)) internal to the sodA gene, encoding the manganese-dependent superoxide dismutase, was amplified and sequenced with a single pair of degenerate primers from the type strains of Pasteurella (18 strains...

journal_title：Journal of clinical microbiology

authors： Gautier AL,Dubois D,Escande F,Avril JL,Trieu-Cuot P,Gaillot O

更新日期：2005-05-01 00:00:00

abstract：:A case of severe progressive subcutaneous abscesses and necrotizing tenosynovitis of the right arm of a 30-year-old woman caused by Rhodococcus aurantiacus is reported. ...

journal_title：Journal of clinical microbiology

authors： Tsukamura M,Hikosaka K,Nishimura K,Hara S

更新日期：1988-02-01 00:00:00

abstract：:Azole-resistant Aspergillus fumigatus is an increasing worldwide problem with major clinical implications. Surveillance is warranted to guide clinicians to provide optimal treatment to patients. To investigate azole resistance in clinical Aspergillus isolates in our institution, a Belgian university hospital, we condu...

journal_title：Journal of clinical microbiology

authors： Montesinos I,Argudín MA,Hites M,Ahajjam F,Dodémont M,Dagyaran C,Bakkali M,Etienne I,Jacobs F,Knoop C,Patteet S,Lagrou K

更新日期：2017-08-01 00:00:00