Comparison of GonoGen, GonoGen II, and MicroTrak direct fluorescent-antibody test with carbohydrate fermentation for confirmation of culture isolates of Neisseria gonorrhoeae.

abstract：:This study evaluated the quantitative results from and quality of the inoculation patterns of urine specimens produced by two automated instruments, the Copan WASP and the BD InoqulA. Five hundred twenty-six urine samples submitted in 10-ml canisters containing boric acid were processed within 30 min on an InoqulA ins...

journal_title：Journal of clinical microbiology

authors： Iversen J,Stendal G,Gerdes CM,Meyer CH,Andersen CØ,Frimodt-Møller N

更新日期：2016-02-01 00:00:00

abstract：:A uracil-to-cytosine mutation at nucleotide position 472 of oral poliovirus vaccine type 3 (OPV3) contributes to the development of vaccine-associated paralytic poliomyelitis (VAPP). To analyze OPV3 shedding patterns, we previously used the multistep method of mutant analysis by PCR and enzyme cleavage (MAPREC). This ...

journal_title：Journal of clinical microbiology

authors： Gnanashanmugam D,Falkovitz-Halpern MS,Dodge A,Fang M,Wong LJ,Esparza M,Hammon R,Rivas-Merelles EE,Santos JI,Maldonado Y

更新日期：2007-08-01 00:00:00

abstract：:Klebsiella pneumoniae has been emerging as the leading cause of liver abscess in diabetic patients. Results of molecular typing of K. pneumoniae isolates from two siblings with liver abscess, their family members, and the environment suggest a possibility of cross infection of liver abscess by the fecal-oral route wit...

journal_title：Journal of clinical microbiology

authors： Chiu CH,Su LH,Wu TL,Hung IJ

更新日期：2001-06-01 00:00:00

abstract：:Ureaplasma urealyticum has been associated with urethritis in men, obstetric problems in women, and respiratory distress syndrome in preterm infants. U. urealyticum can be divided into two biovars comprising 14 serovars. Partial sequences of genes encoding the multiple-banded antigens of the cell surface are known. Us...

journal_title：Journal of clinical microbiology

authors： Pitcher D,Sillis M,Robertson JA

更新日期：2001-05-01 00:00:00

abstract：:Real-time PCR analysis of the total bacterial load in advanced carious lesions has shown that the total load exceeds the number of cultivable bacteria. This suggests that an unresolved complexity exists in bacteria associated with advanced caries. In this report, the profile of the microflora of carious dentine was ex...

journal_title：Journal of clinical microbiology

authors： Chhour KL,Nadkarni MA,Byun R,Martin FE,Jacques NA,Hunter N

更新日期：2005-02-01 00:00:00

abstract：:The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycle...

journal_title：Journal of clinical microbiology

authors： Preus HR,Haraszthy VI,Zambon JJ,Genco RJ

更新日期：1993-10-01 00:00:00

abstract：:This is the first reported case of native valve endocarditis caused by Engyodontium album. This fungus, rarely seen as a human pathogen, is separated from Tritirachium species by its lack of pigmentation and from Beauveria species by the presence of conidiogenous cells in whorls. ...

journal_title：Journal of clinical microbiology

authors： Augustinsky J,Kammeyer P,Husain A,deHoog GS,Libertin CR

更新日期：1990-06-01 00:00:00

abstract：:During 1984, the recovery of enteric pathogens from patients with acute diarrhea was enhanced by the use of both rectal swab and stool specimens. With 513 patients for whom both methods were used, the overall recovery rate was increased a minimum of about 10%. Almost 50% of the organisms recovered were detected by onl...

journal_title：Journal of clinical microbiology

authors： Adkins HJ,Santiago LT

更新日期：1987-01-01 00:00:00

abstract：:A duplex PCR to detect Bordetella pertussis and Bordetella parapertussis was developed with the insertion sequences IS481 (B. pertussis) and IS1001 (B. parapertussis) and evaluated with specimens from 520 consecutive patients presenting with possible pertussis. No culture-positive-PCR-negative results occurred, giving...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Daggard G,Mukkur TK

更新日期：1999-03-01 00:00:00

abstract：:Direct enzyme immunoassay (Herpchek) was compared with culture on 21,522 specimens mainly from asymptomatic women for herpes simplex virus detection. Sensitivity and specificity were 73.8 and 97.7%, respectively. The 33% detection rate by enzyme immunoassay in 5 h increased to 43% when the enzyme immunoassay was combi...

journal_title：Journal of clinical microbiology

authors： Verano L,Michalski FJ

更新日期：1995-05-01 00:00:00

abstract：:Test strips for the detection of antibodies to human immunodeficiency virus type 1 were investigated using specimens from risk groups in Thailand (141 reactive; 445 nonreactive) in a local Thai laboratory. The diagnostic sensitivity and specificity were both 100%. Using a set of seroconversion panels, the sensitivity ...

journal_title：Journal of clinical microbiology

authors： Pagcharoenpol P,Burgess-Cassler A,Schramm W

更新日期：1996-04-01 00:00:00

abstract：:Multi-virulence-locus sequence typing (MVLST) was used to analyze isolates from two major listeriosis outbreaks in the United States in 1998 and 2002 that were due to consumption of contaminated hot dogs and turkey deli meat, respectively. MVLST demonstrated high epidemiological relevance and indicated that the two ou...

journal_title：Journal of clinical microbiology

authors： Chen Y,Zhang W,Knabel SJ

更新日期：2005-10-01 00:00:00

abstract：:We examined the reliability and accuracy of a colorimetric assay using Alamar Blue reagent in the performance of susceptibility tests for Candida albicans. We compared the broth macrodilution method recommended by the National Committee for Clinical Laboratory Standards (NCCLS) with a macrodilution method modified wit...

journal_title：Journal of clinical microbiology

authors： Tiballi RN,He X,Zarins LT,Revankar SG,Kauffman CA

更新日期：1995-04-01 00:00:00

abstract：:Pregnant cows were successfully hyperimmunized with all four human rotavirus serotypes, resulting in a 100-fold increase in neutralizing milk antibody titers over those of controls. Milk antibodies were isolated batchwise from 1,000 kg of pooled milk for the first 10 lactation days, yielding 10 kg of freeze-dried milk...

journal_title：Journal of clinical microbiology

authors： Brüssow H,Hilpert H,Walther I,Sidoti J,Mietens C,Bachmann P

更新日期：1987-06-01 00:00:00

abstract：:Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the p...

journal_title：Journal of clinical microbiology

authors： Somoskovi A,Dormandy J,Parsons LM,Kaswa M,Goh KS,Rastogi N,Salfinger M

更新日期：2007-02-01 00:00:00

abstract：:Phosphatase activity was found to be applicable as a criterion for the differentiation of Mycoplasma salivarium and Mycoplasma orale, predominant constituents of oral mycoplasmal flora. Therefore, a simple procedure for the phosphatase activity assay was established as a screening test for the differentiation of oral ...

journal_title：Journal of clinical microbiology

authors： Shibata K,Totsuka M,Watanabe T

更新日期：1986-05-01 00:00:00

abstract：:Thirteen different serogroup strains of Yersinia pseudotuberculosis and two strains of Yersinia enterocolitica O:3 were examined for the presence of plasmids and plasmid-mediated properties, calcium growth dependency, and autoagglutination. Two Y. enterocolitica strains and eight serogroup (IA, IIA, IIC, III, IVA, VB,...

journal_title：Journal of clinical microbiology

authors： Ishiguro N,Nakaoka Y,Sato G,Tsubokura M

更新日期：1985-04-01 00:00:00

abstract：:Resistance to linezolid is rare in clinical isolates of Enterococcus faecalis. A strain resistant to this antimicrobial but susceptible to vancomycin was found to cause central venous catheter colonization in a patient who never received linezolid. ...

journal_title：Journal of clinical microbiology

authors： Marra AR,Major Y,Edmond MB

更新日期：2006-05-01 00:00:00

abstract：:Accurate and rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) is needed to screen MRSA carriers and improve treatment. The current widely used duplex PCR methods are not able to differentiate MRSA from coexisting methicillin-susceptible S. aureus (MSSA) or other methicillin-resistant staphylo...

journal_title：Journal of clinical microbiology

authors： Luo J,Li J,Yang H,Yu J,Wei H

更新日期：2017-10-01 00:00:00

abstract：:A space-saving media table designed to facilitate pouring large quantities of plated media in a small area is described. ...

journal_title：Journal of clinical microbiology

authors： McGonagle LA,Schmidt G,Eng S,Wirsing K

更新日期：1975-07-01 00:00:00

abstract：:The incidence of vancomycin resistance among enterococci is increasing in the United States and elsewhere in the world, but automated susceptibility testing methods have difficulty detecting resistance expressed by certain strains. The agar screening method described by Willey et al. (B. M. Willey, B. N. Kreiswirth, A...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Clark NC,Ferraro MJ,Sahm DF,Doern G,Pfaller MA,Reller LB,Weinstein MP,Zabransky RJ,Tenover FC

更新日期：1994-07-01 00:00:00

abstract：:Eighty-four pneumococci with various MICs of penicillin (38 with MICs of less than or equal to 0.06 micrograms/ml [susceptible], 35 with MICs of 0.12 to 1.0 micrograms/ml [relatively resistant], and 11 with MICs of greater than 1.0 micrograms/ml [resistant] ) were screened by a disk diffusion test using oxacillin and ...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Hill BC,Thornsberry C

更新日期：1986-11-01 00:00:00

abstract：:We report on a novel strategy for the detection of mRNA targets derived from Cryptosporidium parvum oocysts by the use of oligonucleotide-gold nanoparticles. Gold nanoparticles are functionalized with oligonucleotides which are complementary to unique sequences present on the heat shock protein 70 (HSP70) DNA/RNA targ...

journal_title：Journal of clinical microbiology

authors： Javier DJ,Castellanos-Gonzalez A,Weigum SE,White AC Jr,Richards-Kortum R

更新日期：2009-12-01 00:00:00

abstract：:Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Siqueira LPM,Gimenes VMF,de Freitas RS,Melhem MSC,Bonfietti LX,da Silva AR Jr,Souza Santos LB,Motta AL,Rossi F,Benard G,de Almeida JN Jr

更新日期：2019-01-02 00:00:00

abstract：:A clinical evaluation was conducted in six North American centers to determine the ability of APTIMA CT (ACT) and APTIMA GC (AGC) nucleic acid amplification assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections in 1,322 men by testing their urethral swabs and first-catch urine (FCU). The results ...

journal_title：Journal of clinical microbiology

authors： Chernesky MA,Martin DH,Hook EW,Willis D,Jordan J,Wang S,Lane JR,Fuller D,Schachter J

更新日期：2005-01-01 00:00:00

abstract：:Real-time PCR shows the widespread presence of Coxiella burnetii DNA in a broad range of commercially available milk and milk products. MLVA genotyping shows that this is the result of the presence of a predominant C. burnetii genotype in the dairy cattle population. ...

journal_title：Journal of clinical microbiology

authors： Tilburg JJ,Roest HJ,Nabuurs-Franssen MH,Horrevorts AM,Klaassen CH

更新日期：2012-06-01 00:00:00

abstract：:The effect of supplementing nutrient substrate with various combinations of concentrations of oleic and linoleic acids on the growth of 11 strains of Staphylococcus aureus was assessed. Whereas increasing the concentration of linoleic acid by itself greatly diminished the growth of all 11 strains, concomitant increase...

journal_title：Journal of clinical microbiology

authors： Campbell IM,Crozier DN,Pawagi AB,Buivids IA

更新日期：1983-08-01 00:00:00

abstract：:We report five new IS711 chromosomal locations that are specific for marine mammal Brucella groups of strains and useful for their identification and classification. Our data support their current classification into two species, Brucella ceti and B. pinnipedialis, with subgroups in each, but also the possibility of a...

journal_title：Journal of clinical microbiology

authors： Zygmunt MS,Maquart M,Bernardet N,Doublet B,Cloeckaert A

更新日期：2010-10-01 00:00:00

abstract：:Two cyp51-related genes (cyp51A and cyp51B) encoding 14-alpha sterol demethylase-like enzymes were identified in the opportunistic human pathogen Aspergillus fumigatus. PCR amplification using degenerate oligonucleotides based on conserved areas of cytochrome P450 demethylases of other filamentous fungi and yeasts all...

journal_title：Journal of clinical microbiology

authors： Mellado E,Diaz-Guerra TM,Cuenca-Estrella M,Rodriguez-Tudela JL

更新日期：2001-07-01 00:00:00

abstract：:Using blaZ PCR as the "gold standard," the sensitivities of CLSI penicillin zone edge and nitrocefin-based tests for β-lactamase production in Staphylococcus aureus were 64.5% and 35.5%, respectively, with specificity of 99.8% for both methods. In 2013, 13.5% of 3,083 S. aureus isolates from 31 U.S. centers were penic...

journal_title：Journal of clinical microbiology

authors： Richter SS,Doern GV,Heilmann KP,Miner S,Tendolkar S,Riahi F,Diekema DJ

更新日期：2016-03-01 00:00:00