Ability of new APTIMA CT and APTIMA GC assays to detect Chlamydia trachomatis and Neisseria gonorrhoeae in male urine and urethral swabs.

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently been described as a fast and inexpensive method for the identification of mycobacteria. Although mycobacteria require extraction prior to MALDI-TOF MS analysis, previously published protocols have been relatively c...

journal_title：Journal of clinical microbiology

authors： Mather CA,Rivera SF,Butler-Wu SM

更新日期：2014-01-01 00:00:00

abstract：:Evaluation of previously described chemically defined media for the growth of Legionella pneumophila showed that these media supported poor growth of several strains of L. pneumophila and did not support growth of certain of the Legionella species described later. Growth was stimulated by the dialysate from yeast extr...

journal_title：Journal of clinical microbiology

authors： Pine L,Franzus MJ,Malcolm GB

更新日期：1986-01-01 00:00:00

abstract：:The way in which the extensive use of highly active antiretroviral therapy (HAART) has influenced the incidence of visceral leishmaniasis (VL) among human immunodeficiency type 1 (HIV-1)-infected patients is not yet understood. The present study assessed whether the incidence of symptomatic VL in HIV-infected patients...

journal_title：Journal of clinical microbiology

authors： de La Rosa R,Pineda JA,Delgado J,Macías J,Morillas F,Mira JA,Sánchez-Quijano A,Leal M,Lissen E

更新日期：2002-03-01 00:00:00

abstract：:We have shown that a modification of the color-coded antigen developed by Kasatiya and Lambert (Appl. Microbiol. 28:317-318, 1974) can be used as a substitute for other nontreponemal antigens used in screening tests for syphilis. The antigen is based on the Venereal Disease Research Laboratory antigen, with EDTA, chol...

journal_title：Journal of clinical microbiology

authors： Pettit DE,Larsen SA,Harbec PS,Feeley JC,Parham CE,Cruce DD,Hambie EA,Perryman MW

更新日期：1983-11-01 00:00:00

abstract：:Colonization of the gastrointestinal and genitourinary tracts of pregnant women with group B Streptococcus (GBS) can result in vertical transmission to neonates during labor/delivery. GBS infections in neonates can cause severe complications, such as sepsis, meningitis, and pneumonia. Accurate detection is critical be...

journal_title：Journal of clinical microbiology

authors： Shin JH,Pride DT

更新日期：2019-05-24 00:00:00

abstract：:Although two phenotypes of the opportunistic pathogen Propionibacterium acnes (types I and II) have been described, epidemiological investigations of their roles in different infections have not been widely reported. Using immunofluorescence microscopy with monoclonal antibodies (MAbs) QUBPa1 and QUBPa2, specific for ...

journal_title：Journal of clinical microbiology

authors： McDowell A,Valanne S,Ramage G,Tunney MM,Glenn JV,McLorinan GC,Bhatia A,Maisonneuve JF,Lodes M,Persing DH,Patrick S

更新日期：2005-01-01 00:00:00

abstract：:We compared examination of unstained and Gram-stained smears as methods of evaluating sputum quality. Of 100 slides examined, 96 (96%) were graded identically. Each discrepancy between methods was near the cutoff for specimen acceptability. We concluded that examination of unstained smears can provide an accurate, cos...

journal_title：Journal of clinical microbiology

authors： Wetterau LM,Zeimis RT,Hollick GE

更新日期：1986-07-01 00:00:00

abstract：:Nineteen isolates of leptospires recovered from patients during three epidemics that occurred at different places and different times in the Andaman Islands and eight isolates from sporadic cases were characterized using serological and molecular genetic techniques. Group sera and monoclonal antibodies were used for a...

journal_title：Journal of clinical microbiology

authors： Vijayachari P,Ahmed N,Sugunan AP,Ghousunnissa S,Rao KR,Hasnain SE,Sehgal SC

更新日期：2004-08-01 00:00:00

abstract：:Optimal conditions for a fluorescence immunoassay of antibodies to, and surface antigens of, Actinomyces viscosus ATCC 19246 are described. In the standard fluorescence immunoassay, 10(8) colony-forming units of A. viscosus reacted with an antibody preparation, were washed, and then were treated with an excess of fluo...

journal_title：Journal of clinical microbiology

authors： Gillis TP,Thompson JJ

更新日期：1978-02-01 00:00:00

abstract：:The genotypes of 63 isolates of Aspergillus fumigatus obtained from three hospitals in different geographical areas and of eight culture collection strains were determined by multilocus enzyme electrophoresis. Twelve of the 17 enzymatic loci studied were polymorphic, giving rise to 48 different electrophoretic types. ...

journal_title：Journal of clinical microbiology

authors： Rodriguez E,De Meeüs T,Mallie M,Renaud F,Symoens F,Mondon P,Piens MA,Lebeau B,Viviani MA,Grillot R,Nolard N,Chapuis F,Tortorano AM,Bastide JM

更新日期：1996-10-01 00:00:00

abstract：:Botulism developed in a patient following surgical repair of an open radial fracture. Symptoms resolved after treatment with antitoxin and antibiotics, and hardware excision was deferred. Subsequent osteomyelitis necessitated hardware exchange, and wound cultures grew Clostridium argentinense. This case highlights the...

journal_title：Journal of clinical microbiology

authors： Taylor SM,Wolfe CR,Dixon TC,Ruch DS,Cox GM

更新日期：2010-02-01 00:00:00

abstract：:A variety of fungi produce the hydrolytic enzyme beta-N-acetylhexosaminidase (HexNAcase), which can be readily detected in assays by using p-nitrophenyl-N-acetyl-beta-D-glucosaminide as a substrate. In the present study we developed a microtiter plate-based HexNAcase assay for distinguishing Candida albicans and Candi...

journal_title：Journal of clinical microbiology

authors： Niimi K,Shepherd MG,Cannon RD

更新日期：2001-06-01 00:00:00

abstract：:Monoclonal antibodies reactive with three different viral polypeptides were evaluated singly and in combination as the capture antibody(s) in an enzyme-linked immunosorbent assay system for the detection of bovine enteric coronavirus. Similar levels of sensitivity were found for all combinations tested. A sensitive, h...

journal_title：Journal of clinical microbiology

authors： Crouch CF,Raybould TJ,Acres SD

更新日期：1984-03-01 00:00:00

abstract：:Production of staphylococcal alpha- or alpha-beta-toxins correlated well with production of coagulase or thermonuclease (or both) in 203 Staphylococcus aureus isolates from milk and should be reliable indicators of S. aureus in the absence of Staphylococcus intermedius. Failures to produce toxin, tube coagulase, or th...

journal_title：Journal of clinical microbiology

authors： Jasper DE,Infante F,Dellinger JD

更新日期：1985-04-01 00:00:00

abstract：:By quantitative gas-liquid chromatography of glucose-containing blood cultures at the moment of first signs of growth, a presumptive diagnosis of anaerobic bacteremia could be made in 24 out of 26 cultures yielding obligate anaerobes upon subsequent culture. With Bacteroides sp. (20 strains isolated), elevated levels ...

journal_title：Journal of clinical microbiology

authors： Wüst J

更新日期：1977-12-01 00:00:00

abstract：:Staphylococcal cassette chromosome mec (SCCmec) types and Panton-Valentine leukocidin (PVL) gene carriage were compared among suspected community-associated methicillin-resistant Staphylococcus aureus MRSA (CA-MRSA) and health care-associated MRSA (HA-MRSA) isolates. CA-MRSA isolates carried the SCCmec type IV complex...

journal_title：Journal of clinical microbiology

authors： Moroney SM,Heller LC,Arbuckle J,Talavera M,Widen RH

更新日期：2007-03-01 00:00:00

abstract：:The performance of a disk diffusion test using broth from positive blood cultures as inoculum (direct disk diffusion [dDD]) was evaluated for a collection of 20 challenge isolates of Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa Isolates seeded into human blood were inoculated into Bactec Plu...

journal_title：Journal of clinical microbiology

authors： Chandrasekaran S,Abbott A,Campeau S,Zimmer BL,Weinstein M,Thrupp L,Hejna J,Walker L,Ammann T,Kirn T,Patel R,Humphries RM

更新日期：2018-02-22 00:00:00

abstract：:We compared the Gen-Probe transcription-mediated amplification assay (AMP CT), the Abbott LCx assay, and the Roche COBAS AMPLICOR assay for the detection of Chlamydia trachomatis in a mixed population in urine samples. First-void urine, urethral specimens, and cervical specimens in females were obtained from 1,000 pat...

journal_title：Journal of clinical microbiology

authors： Goessens WH,Mouton JW,van der Meijden WI,Deelen S,van Rijsoort-Vos TH,Lemmens-den Toom N,Verbrugh HA,Verkooyen RP

更新日期：1997-10-01 00:00:00

abstract：:We retrospectively analyzed epidemiologic information associated with 22 cultures of Neisseria elongata subsp. nitroreducens (formerly CDC group M-6) submitted to the Microbial Diseases Laboratory, California Department of Health Services, Berkeley, over a 16-year period. The most common illnesses noted with this bact...

journal_title：Journal of clinical microbiology

authors： Wong JD,Janda JM

更新日期：1992-03-01 00:00:00

abstract：:A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuab...

journal_title：Journal of clinical microbiology

authors： Prod'hom G,Guilhot C,Gutierrez MC,Varnerot A,Gicquel B,Vincent V

更新日期：1997-12-01 00:00:00

abstract：:Analysis of the electrophoretic profiles of the outer membrane proteins could be used to differentiate Campylobacter jejuni (16 strains) from Campylobacter coli (10 strains). This observation was confirmed by the study of DNA homology obtained by a quantitative filter hybridization method. The hippurate hydrolysis tes...

journal_title：Journal of clinical microbiology

authors： Derclaye I,Delor I,Van Bouchaute M,Moureau P,Wauters G,Cornelis GR

更新日期：1989-05-01 00:00:00

abstract：:Large hospital-based clinical laboratories must be prepared to rapidly investigate potential infectious disease outbreaks. To challenge the ability of our molecular diagnostics laboratory to use whole-genome sequencing in a potential outbreak scenario and identify impediments to these efforts, we studied 84 invasive s...

journal_title：Journal of clinical microbiology

authors： Olsen RJ,Fittipaldi N,Kachroo P,Sanson MA,Long SW,Como-Sabetti KJ,Valson C,Cantu C,Lynfield R,Van Beneden C,Beres SB,Musser JM

更新日期：2014-12-01 00:00:00

abstract：:Ten clinical yeast isolates submitted to the Centers for Disease Control from diverse geographic areas were identified as Candida ciferrii and Candida chiropterorum. The association of C. ciferrii with clinical specimens, particularly its repeated isolation from a case of onychomycosis, suggests that this species may ...

journal_title：Journal of clinical microbiology

authors： Furman RM,Ahearn DG

更新日期：1983-11-01 00:00:00

abstract：:An ATP-based biocide susceptibility assay for mycobacteria was developed by optimizing the cell lysis and assay conditions. Compared to the conventional agar plating method, the assay was rapid (1.5 h) and showed high sensitivity and specificity as determined by receiver operating characteristic (ROC) analysis. The te...

journal_title：Journal of clinical microbiology

authors： Kapoor R,Yadav JS

更新日期：2010-10-01 00:00:00

abstract：:Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced ...

journal_title：Journal of clinical microbiology

authors： Appuhamy S,Coote JG,Low JC,Parton R

更新日期：1998-03-01 00:00:00

abstract：:The emergence of extended-spectrum beta-lactamase (ESBL) and plasmid-mediated AmpC (pAmpC) enzymes in Escherichia coli raises concern regarding accurate laboratory detection and interpretation of susceptibility testing results. Twenty-six cefpodoxime ESBL screen-positive, cefoxitin-resistant E. coli clinical isolates ...

journal_title：Journal of clinical microbiology

authors： Robberts FJ,Kohner PC,Patel R

更新日期：2009-02-01 00:00:00

abstract：:In order to increase the database for in vitro growth and/or susceptibility testing in liquid media, we evaluated the growth of Helicobacter pylori in broth media containing 5% sheep blood. We also compared the effect of bismuth on the growth of H. pylori in broth media containing 10% fetal calf serum with the effect ...

journal_title：Journal of clinical microbiology

authors： Coudron PE,Stratton CW

更新日期：1995-04-01 00:00:00

abstract：:During a 15-month period, two methods for detection of herpes simplex virus (HSV) in 699 clinical specimens were compared: (i) 24-well-plate centrifugation (24WPC) with MRC-5 cells and staining with type-specific monoclonal antibodies (Syva Co., Palo Alto, Calif.) after incubation for 16 to 18 h and (ii) conventional ...

journal_title：Journal of clinical microbiology

authors： Woods GL,Mills RD

更新日期：1988-03-01 00:00:00

abstract：:Quantitative PCR (qPCR) is more sensitive than microscopy for detecting Pneumocystis jirovecii in bronchoalveolar lavage (BAL) fluid. We therefore developed a qPCR assay and compared the results with those of a routine immunofluorescence assay (IFA) and clinical data. The assay included automated DNA extraction, ampli...

journal_title：Journal of clinical microbiology

authors： Botterel F,Cabaret O,Foulet F,Cordonnier C,Costa JM,Bretagne S

更新日期：2012-02-01 00:00:00

abstract：:Whether targeted or universal decolonization strategies for the control of methicillin-resistant Staphylococcus aureus (MRSA) select for resistance to decolonizing agents is unresolved. The REDUCE-MRSA trial (ClinicalTrials registration no. NCT00980980) provided an opportunity to investigate this question. REDUCE-MRSA...

journal_title：Journal of clinical microbiology

authors： Hayden MK,Lolans K,Haffenreffer K,Avery TR,Kleinman K,Li H,Kaganov RE,Lankiewicz J,Moody J,Septimus E,Weinstein RA,Hickok J,Jernigan J,Perlin JB,Platt R,Huang SS

更新日期：2016-11-01 00:00:00