Development of a rapid ATP bioluminescence assay for biocidal susceptibility testing of rapidly growing mycobacteria.

Abstract:

:An ATP-based biocide susceptibility assay for mycobacteria was developed by optimizing the cell lysis and assay conditions. Compared to the conventional agar plating method, the assay was rapid (1.5 h) and showed high sensitivity and specificity as determined by receiver operating characteristic (ROC) analysis. The test species, Mycobacterium immunogenum, M. chelonae, and M. abscessus, showed various susceptibilities to the glutaraldehyde- and isothiazolone-based test biocides.

journal_name

J Clin Microbiol

authors

Kapoor R,Yadav JS

doi

10.1128/JCM.01482-10

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

3725-8

issue

10

eissn

0095-1137

issn

1098-660X

pii

JCM.01482-10

journal_volume

48

pub_type

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