Comparison of four plating media for isolating Vibrio cholerae.

abstract：:Clostridium difficile is the bacterium most commonly surmised to cause antimicrobial- and hospital-associated diarrhea in developed countries worldwide, and such infections are thought to be increasing in frequency and severity. A laboratory-based study was carried out to characterize C. difficile strains isolated fro...

journal_title：Journal of clinical microbiology

authors： Martin H,Willey B,Low DE,Staempfli HR,McGeer A,Boerlin P,Mulvey M,Weese JS

更新日期：2008-09-01 00:00:00

abstract：:The Sensititre Breakpoint Autoreader system (SBAS) is a broth microdilution method with one to three concentrations of each antibiotic and innovative fluorescence technology to define inhibitory endpoints. We tested 248 gram-negative bacilli and 80 gram-positive cocci using both the rapid (5 h) and overnight (18 h) SB...

journal_title：Journal of clinical microbiology

authors： Nolte FS,Krisher KK,Beltran LA,Christianson NP,Sheridan GE

更新日期：1988-06-01 00:00:00

abstract：:Hepatitis G virus (HGV) was recently identified as a new member of the Flaviviridae, but its clinical significance is still unclear. Since no immunoassay for the diagnosis of HGV is available, we developed a sensitive reverse transcription-PCR (RT-PCR) assay to facilitate the detection of the viral genome by mass scre...

journal_title：Journal of clinical microbiology

authors： Schlueter V,Schmolke S,Stark K,Hess G,Ofenloch-Haehnle B,Engel AM

更新日期：1996-11-01 00:00:00

abstract：:Nasopharyngeal swabs (NPSs) are being widely used as specimens for multiplex real-time reverse transcription (RT)-PCR for respiratory virus detection. However, it remains unclear whether NPS specimens are optimal for all viruses targeted by multiplex RT-PCR. In addition, the procedure to obtain NPS specimens causes co...

journal_title：Journal of clinical microbiology

authors： Kim YG,Yun SG,Kim MY,Park K,Cho CH,Yoon SY,Nam MH,Lee CK,Cho YJ,Lim CS

更新日期：2016-12-28 00:00:00

abstract：:Of 12,321 stool samples analyzed over a 6-year interval, 870 (7.1%) were positive for a total of 1,019 parasites, of which 1,011 (99.2%) were found in trichrome-stained smears of unconcentrated specimens while only 479 (47.0%) were detected in iodine-stained smears of concentrated samples. Stool specimens were next an...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Elder CJ

更新日期：1999-03-01 00:00:00

abstract：:Helicobacter pylori cagA and vacA genotypes have been used for almost a decade as stable entities to link the severity of gastritis and ulcer disease. We describe here microevolution of the two genomic islands, cag pathogenicity island (cagPAI; 40 kb) and tfs3 (16 kb) from isolates obtained at inclusion (one subclone)...

journal_title：Journal of clinical microbiology

authors： Alvi A,Devi SM,Ahmed I,Hussain MA,Rizwan M,Lamouliatte H,Mégraud F,Ahmed N

更新日期：2007-12-01 00:00:00

abstract：:Invasive pulmonary aspergillosis (IPA) is a frequently fatal infection in immunocompromised patients that is difficult to diagnose. Present methods for detection of Aspergillus spp. in bronchoalveolar lavage (BAL) fluid and in tissue vary in sensitivity and specificity. We therefore developed an A. fumigatus-specific ...

journal_title：Journal of clinical microbiology

authors： O'Sullivan CE,Kasai M,Francesconi A,Petraitis V,Petraitiene R,Kelaher AM,Sarafandi AA,Walsh TJ

更新日期：2003-12-01 00:00:00

abstract：:Cryptococcus gattii emerged in North America in 1999 as a human and veterinary pathogen on Vancouver Island, British Columbia. The emergent subtype, VGIIa, and the closely related subtype VGIIb can now be found in the United States in Washington, Oregon, and California. We performed multilocus sequence typing and anti...

journal_title：Journal of clinical microbiology

authors： Iqbal N,DeBess EE,Wohrle R,Sun B,Nett RJ,Ahlquist AM,Chiller T,Lockhart SR,Cryptococcus gattii Public Health Working Group.

更新日期：2010-02-01 00:00:00

abstract：:Determination of the G and P serotypes of group A bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-PCR typing assay. The G6 serotype was the most prevalent, accounting for viruses in 55....

journal_title：Journal of clinical microbiology

authors： Falcone E,Tarantino M,Di Trani L,Cordioli P,Lavazza A,Tollis M

更新日期：1999-12-01 00:00:00

abstract：:Influenza viruses related to the markedly antigenically divergent strains B/Yamagata/16/88 and B/Victoria/2/87 are circulating in human populations. Adults develop cross-reacting antibodies against recent and earlier influenza B virus strains after vaccination with B/Yamagata/16/88, probably because of previous influe...

journal_title：Journal of clinical microbiology

authors： Levandowski RA,Gross PA,Weksler M,Staton E,Williams MS,Bonelli J

更新日期：1991-07-01 00:00:00

abstract：:We used several molecular typing methods to analyze 196 methicillin-resistant Staphylococcus aureus (MRSA) and 139 methicillin-susceptible S. aureus (MSSA) isolates collected between 1996 and 2005. The sequence type 72 MRSA has increased in frequency in the community in the Republic of Korea and in hospitals in recent...

journal_title：Journal of clinical microbiology

authors： Kim ES,Lee HJ,Chung GT,Lee YS,Shin DH,Jung SI,Song KH,Park WB,Kim NJ,Park KU,Kim EC,Oh MD,Kim HB

更新日期：2011-05-01 00:00:00

abstract：:New blood culture media containing antibiotic-binding polymeric beads have been developed for the BacT/Alert (bioMérieux, Inc., Durham, NC) blood culture system. To assess the performance of these new media, we compared the new BacT/Alert aerobic medium (FA Plus) with resins to BacT/Alert FA medium with activated char...

journal_title：Journal of clinical microbiology

authors： Kirn TJ,Mirrett S,Reller LB,Weinstein MP

更新日期：2014-03-01 00:00:00

abstract：:The development of a satisfactory means to reliably distinguish between the two closely related species Candida albicans and Candida dubliniensis in the clinical mycology laboratory has proved difficult because these two species are phenotypically so similar. In this study, we have detected homologues of the pH-regula...

journal_title：Journal of clinical microbiology

authors： Kurzai O,Heinz WJ,Sullivan DJ,Coleman DC,Frosch M,Mühlschlegel FA

更新日期：1999-05-01 00:00:00

abstract：:The performance of four media for the isolation of Aeromonas strains from stool specimens, the importance of ampicillin-susceptible Aeromonas strains in the selection of culture media, and the usefulness of beta-hemolysis in screening blood-containing media for Aeromonas strains were evaluated in two phases. In the fi...

journal_title：Journal of clinical microbiology

authors： Kelly MT,Stroh EM,Jessop J

更新日期：1988-09-01 00:00:00

abstract：:Two colorimetric methods that use Alamar Blue or 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) for assaying the in vitro activities of antifungal agents have been described. We report that both tests performed similarly when the antifungal activity of amphotericin B against Candida albicans wa...

journal_title：Journal of clinical microbiology

authors： Jahn B,Stüben A,Bhakdi S

更新日期：1996-08-01 00:00:00

abstract：:A total of 122 adenovirus type 4 (Ad4) strains isolated from patients with epidemic keratoconjunctivitis at an eye clinic in Sapporo, Japan, from 1985 to 1989 were identified as Ad4a. By the use of HinfI and TaqI restriction endonucleases, these Ad4a strains were classified into 12 subgenome types. During this observa...

journal_title：Journal of clinical microbiology

authors： Itakura S,Aoki K,Sawada H,Ishiguro N,Shinagawa M

更新日期：1991-08-01 00:00:00

abstract：:The PNA-FISH Yeast Traffic Light assay was performed on 54 clinical isolates of yeasts inoculated into blood culture bottles. The assay showed high sensitivity (Candida albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 92.3%; C. tropicalis, 100%) and specificity (C. albicans/C. parapsilosis, 100%; C. glabrata/C. ...

journal_title：Journal of clinical microbiology

authors： Stone NR,Gorton RL,Barker K,Ramnarain P,Kibbler CC

更新日期：2013-04-01 00:00:00

abstract：:We previously demonstrated the transmission of murine cytomegalovirus to syngeneic mice and preformed monolayers of mouse embryo fibroblasts with plastic-adherent peritoneal exudate cells from infected mice as a source of macrophages. In the present studies we compared this standard feeder layer method with a reverse ...

journal_title：Journal of clinical microbiology

authors： Winkler SR,Booss J

更新日期：1980-12-01 00:00:00

abstract：:A surveillance network was implemented by the Istituto Superiore di Sanità of Rome in collaboration with laboratories of virology in Czech Republic, Slovenia, Croatia, Albania, and Bulgaria. About 1,500 rotavirus-positive stool samples were collected from children with severe gastroenteritis admitted to hospitals or o...

journal_title：Journal of clinical microbiology

authors： Tcheremenskaia O,Marucci G,De Petris S,Ruggeri FM,Dovecar D,Sternak SL,Matyasova I,Dhimolea MK,Mladenova Z,Fiore L,Rotavirus Study Group.

更新日期：2007-07-01 00:00:00

abstract：:A laboratory-based surveillance network of 11 clinical virological laboratories for influenza viruses was established in Taiwan under the coordination of the Center for Disease Control and Prevention (CDC), Taiwan. From October 2000 to March 2004, 3,244 influenza viruses were isolated, including 1,969 influenza A and ...

journal_title：Journal of clinical microbiology

authors： Shih SR,Chen GW,Yang CC,Yang WZ,Liu DP,Lin JH,Chiu SC,Chen HY,Tsao KC,Huang CG,Huang YL,Mok CK,Chen CJ,Lin TY,Wang JR,Kao CL,Lin KH,Chen LK,Eng HL,Liu YC,Chen PY,Lin JS,Wang JH,Lin CW,Chan YJ,Lu JJ,Hsi

更新日期：2005-04-01 00:00:00

abstract：:We evaluated the abilities of three different avian influenza A viruses to attenuate the wild-type human influenza A/Korea/1/82 (H3N2) virus in squirrel monkeys, chimpanzees, and adult seronegative human volunteers. Two of these, avian influenza A/Mallard/NY/78 and A/Mallard/Alberta/76 viruses, appeared to be satisfac...

journal_title：Journal of clinical microbiology

authors： Snyder MH,Clements ML,Herrington D,London WT,Tierney EL,Murphy BR

更新日期：1986-09-01 00:00:00

abstract：:We describe a multiplex PCR assay to identify and discriminate between isolates of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis. The C. jejuni isolate F38011 lpxA gene, encoding a UDP-N-acetylglucosamine acyltransferase, was identified by sequence analysis of an expressio...

journal_title：Journal of clinical microbiology

authors： Klena JD,Parker CT,Knibb K,Ibbitt JC,Devane PM,Horn ST,Miller WG,Konkel ME

更新日期：2004-12-01 00:00:00

abstract：:We investigated the performance of a double-antigen sandwich recombinant enzyme immunoassay (EIA; Abbott Laboratories, North Chicago, Ill.) and compared it with that of a synthetic-peptide-based EIA (Biochem Immunosystems, Montreal, Quebec, Canada) for the detection of human immunodeficiency type 1 (HIV-1) and HIV-2 a...

journal_title：Journal of clinical microbiology

authors： Galli RA,Castriciano S,Fearon M,Major C,Choi KW,Mahony J,Chernesky M

更新日期：1996-04-01 00:00:00

abstract：:Contamination of broth cultures of acid-fast bacilli (AFB) by bacterial species other than Mycobacterium species frequently occurs. Many of these contaminated cultures require redecontamination and reincubation before the appropriate tests can be performed for identification, significantly affecting the turnaround tim...

journal_title：Journal of clinical microbiology

authors： Zheng X,Pang M,Engler HD,Tanaka S,Reppun T

更新日期：2001-10-01 00:00:00

abstract：:HIV-1 drug resistance (HIVDR) assays are important tools in clinical management of HIV-infected patients on antiretroviral therapy (ART) and surveillance of drug-resistant variants at population levels. The high cost associated with commercial assays hinders their use in resource-limited settings. We adopted and valid...

journal_title：Journal of clinical microbiology

authors： Inzaule S,Yang C,Kasembeli A,Nafisa L,Okonji J,Oyaro B,Lando R,Mills LA,Laserson K,Thomas T,Nkengasong J,Zeh C

更新日期：2013-02-01 00:00:00

abstract：:This study evaluated the feasibility of a molecular strategy based on identification of Mycobacterium tuberculosis by IS6110 PCR or Cobas Amplicor PCR, and rpoB PCR-DNA sequencing of the 81-bp rifampin resistance determining region (RRDR) for direct detection of rifampin resistance in respiratory specimens. A collecti...

journal_title：Journal of clinical microbiology

authors： Yam WC,Tam CM,Leung CC,Tong HL,Chan KH,Leung ET,Wong KC,Yew WW,Seto WH,Yuen KY,Ho PL

更新日期：2004-10-01 00:00:00

abstract：:Mupirocin is a topical antimicrobial used to eradicate methicillin-resistant Staphylococcus aureus (MRSA) colonization, usually in the absence of susceptibility testing. We hypothesized that high-level (HL) mupirocin resistance was associated with multidrug resistance (MDR). To this end, unique patient isolates identi...

journal_title：Journal of clinical microbiology

authors： Cadilla A,David MZ,Daum RS,Boyle-Vavra S

更新日期：2011-01-01 00:00:00

abstract：:The recent Zaire Ebola virus (EBOV) outbreak in West Africa illustrates clearly the need for additional studies with humans and animals to elucidate the ecology of Ebola viruses (EBVs). In this study, we developed a serological assay based on the Luminex technology. Nine recombinant proteins representing different vir...

journal_title：Journal of clinical microbiology

authors： Ayouba A,Touré A,Butel C,Keita AK,Binetruy F,Sow MS,Foulongne V,Delaporte E,Peeters M

更新日期：2016-12-28 00:00:00

abstract：:A standardized broth microdilution method was compared to the E test and an agar dilution method for the antimicrobial susceptibility testing of Campylobacter jejuni and C. coli isolates. A group of 47 human clinical isolates, 37 isolates from retail poultry, and 29 isolates from living turkeys (total, 113 isolates) w...

journal_title：Journal of clinical microbiology

authors： Luber P,Bartelt E,Genschow E,Wagner J,Hahn H

更新日期：2003-03-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for detection of feline leukemia virus (FeLV) p27 in saliva was tested for its accuracy and sensitivity in diagnosing FeLV infections. Saliva and serum samples from 564 clinical cases were tested with a 99.2% specificity. The overall accuracy of the saliva ELISA reactive to...

journal_title：Journal of clinical microbiology

authors： Lewis MG,Wright KA,Lafrado LJ,Shanker PJ,Palumbo NE,Lemoine ED,Olsen RG

更新日期：1987-07-01 00:00:00