Francisella tularensis Periprosthetic Joint Infections Diagnosed with Growth in Cultures.

abstract：:Given that the integration of human papillomavirus type 16 (HPV16) into the host genome occurs preferentially with the disruption of the E2 gene, a ratio of E2 to E7 gene copies is often used as a marker for integration. It is largely undetermined, however, whether ratio estimates are affected by HPV intratypic variat...

journal_title：Journal of clinical microbiology

authors： Jiang M,Baseman JG,Koutsky LA,Feng Q,Mao C,Kiviat NB,Xi LF

更新日期：2009-03-01 00:00:00

abstract：:Time to detection (TTD) in automated blood culture systems is delayed for sensitive microorganisms in the presence of antimicrobial substances and has been associated with worse outcomes for sepsis patients on inadequate empirical therapy. While resin addition removes antimicrobial substances to various degrees from b...

journal_title：Journal of clinical microbiology

authors： Mitteregger D,Barousch W,Nehr M,Kundi M,Zeitlinger M,Makristathis A,Hirschl AM

更新日期：2013-05-01 00:00:00

abstract：:The increasing incidence of invasive aspergillosis, a life-threatening infection in immunocompromised patients, emphasizes the need to improve the diagnostic tools for this disease. We established a LightCycler-based real-time PCR assay to detect and quantify rapidly, specifically, and sensitively Aspergillus fumigatu...

journal_title：Journal of clinical microbiology

authors： Spiess B,Buchheidt D,Baust C,Skladny H,Seifarth W,Zeilfelder U,Leib-Mösch C,Mörz H,Hehlmann R

更新日期：2003-05-01 00:00:00

abstract：:A 55-year-old man with severe peripheral vascular disease developed nosocomial septicemia which was caused by the gram-negative bacterium CDC group IV c-2, presumably from a plantar abscess on the left foot. Recovery followed amputation of the infected extremity and antibiotic therapy. This is the first reported case ...

journal_title：Journal of clinical microbiology

authors： Crowe HM,Brecher SM

更新日期：1987-11-01 00:00:00

abstract：:Tularemia is the zoonotic disease caused by the gram-negative coccobacillus Francisella tularensis. Its wide distribution in the environment poses a challenge for understanding the transmission, ecology, and epidemiology of the disease. F. tularensis is also considered a potential biological weapon due to its extreme ...

journal_title：Journal of clinical microbiology

authors： Versage JL,Severin DD,Chu MC,Petersen JM

更新日期：2003-12-01 00:00:00

abstract：:The CLSI (formerly NCCLS) M38-A document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. A multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (MICs and m...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A,Fothergill A,Ghannoum M,Manavathu E,Ostrosky-Zeichner L,Pfaller MA,Rinaldi MG,Schell W,Walsh TJ

更新日期：2007-07-01 00:00:00

abstract：:Acute viral gastroenteritis is an intestinal infection that can be caused by several different viruses. Here we describe the evaluation and verification of Seeplex Diarrhea-V ACE (Seeplex DV), a novel commercial multiplex reverse transcription-PCR (RT-PCR) assay that detects 5 diarrheal pathogens, including adenovirus...

journal_title：Journal of clinical microbiology

authors： Higgins RR,Beniprashad M,Cardona M,Masney S,Low DE,Gubbay JB

更新日期：2011-09-01 00:00:00

abstract：:Quantitative lactate determinations were performed on cerebrospinal fluids to assess their value in the rapid diagnosis of bacterial and mycotic meningitis and to evaluate their value in assessing the prognosis in these patients. Cerebrospinal fluid lactate concentrations were elevated in all patients with untreated b...

journal_title：Journal of clinical microbiology

authors： Komorowski RA,Farmer SG,Hanson GA,Hause LL

更新日期：1978-07-01 00:00:00

abstract：:The BACTEC NR660 blood culture system, which uses infrared spectroscopy to detect carbon dioxide generated by bacterial growth, was compared with the new medium formulation of the Oxoid Signal system. Two trials were conducted: a comparative study of 88 organisms in simulated blood cultures and a clinical trial of 3,3...

journal_title：Journal of clinical microbiology

authors： Daley C,Lim I,Modra J,Wilkinson I

更新日期：1990-07-01 00:00:00

abstract：:Fifty-three isolates of Streptococcus uberis from various sources were examined for the presence of a common antigen. Initially, a serum was produced in rabbits which, by using rocket line immunoelectrophoresis, proved to react with identity to all of the S. uberis crude extracts as well as group B and E streptococcal...

journal_title：Journal of clinical microbiology

authors： Jones KF,Norcross NL

更新日期：1983-05-01 00:00:00

abstract：:Carbohydrate antigen detection, nucleic acid probe detection, and bacterial culture are commonly used to confirm group A streptococcus (GAS) pharyngitis. Compared to standard throat swab specimens, the sensitivities of these tests with mouth specimens are poor. When testing for GAS pharyngitis, the throat remains the ...

journal_title：Journal of clinical microbiology

authors： Fox JW,Marcon MJ,Bonsu BK

更新日期：2006-07-01 00:00:00

abstract：:We describe a multiplex allele-specific (MAS)-PCR assay to detect simultaneously mutations in the first and third bases of the embB gene codon 306ATG. These mutations are known to confer ethambutol (EMB) resistance in the majority of clinical Mycobacterium tuberculosis isolates worldwide. The mutated bases are reveale...

journal_title：Journal of clinical microbiology

authors： Mokrousov I,Narvskaya O,Limeschenko E,Otten T,Vyshnevskiy B

更新日期：2002-05-01 00:00:00

abstract：:Cowdria ruminantium causes severe, often fatal disease in domestic ruminants, whereas wildlife species usually are not affected. Blood and bone marrow samples from healthy, free-ranging Zimbabwean ungulates were taken during translocation from areas harboring Amblyomma ticks and tested for the presence of C. ruminanti...

journal_title：Journal of clinical microbiology

authors： Kock ND,van Vliet AH,Charlton K,Jongejan F

更新日期：1995-09-01 00:00:00

abstract：:Twenty-two isolates of St. Louis encephalitis (SLE) virus of various geographical origins (Brazil, Argentina, Panama, Texas, Missouri, Maryland, California, and Florida) were examined for genetic variation by the base excision sequence scanning (BESS T-scan) method. A fragment was amplified in the envelope gene with t...

journal_title：Journal of clinical microbiology

authors： Charrel RN,Lévy N,Tesh RB,Chandler LJ

更新日期：1999-06-01 00:00:00

abstract：:Microsporidia are protozoa parasites responsible for significant gastrointestinal disease in patients infected with human immunodeficiency virus. We evaluated a PCR assay of stool samples, duodenal aspirates, and biopsy specimens from patients with Enterocytozoon bieneusi infection. A 210-bp DNA fragment of the unique...

journal_title：Journal of clinical microbiology

authors： Velásquez JN,Carnevale S,Guarnera EA,Labbé JH,Chertcoff A,Cabrera MG,Rodríguez MI

更新日期：1996-12-01 00:00:00

abstract：:Malignant catarrhal fever (MCF) was diagnosed by clinical signs and lesions in five out of six white-tailed deer (Odocoileus virginianus) in a North American zoo. The clinical signs and histopathological lesions in these deer were typical of MCF. Antibody to an epitope conserved among the MCF viruses was detected in t...

journal_title：Journal of clinical microbiology

authors： Li H,Dyer N,Keller J,Crawford TB

更新日期：2000-04-01 00:00:00

abstract：:A transferable plasmid encoding SHV-12 extended-spectrum beta-lactamase, TEM-116, and aminoglycoside resistance was responsible for two sequential clonal outbreaks of Enterobacter cloacae and Acinetobacter baumannii bacteria. A similar plasmid was present among isolates of four different bacterial species. Recognition...

journal_title：Journal of clinical microbiology

authors： Naiemi NA,Duim B,Savelkoul PH,Spanjaard L,de Jonge E,Bart A,Vandenbroucke-Grauls CM,de Jong MD

更新日期：2005-09-01 00:00:00

abstract：:Real-time quantitative PCR (qPCR) assay of sputum or nasopharyngeal specimens has shown promising results in the detection of pneumococcal community-acquired pneumonia (PncCAP). We applied qPCR for the autolysin gene (lytA) and compared sputum and nasopharyngeal swab (NPS) pneumococcal loads in elderly patients with c...

journal_title：Journal of clinical microbiology

authors： Saukkoriipi A,Palmu AA,Pascal T,Verlant V,Hausdorff WP,Jokinen J

更新日期：2017-12-26 00:00:00

abstract：:The specificity of Mycobacterium kansasii anti-mycoside A antiserum prepared in rabbits injected with purified samples of the phenolic glycolipid was evaluated by an enzyme-linked immunosorbent assay. Chloroform-methanol extracts from representative strains of 23 mycobacterial species and 50 strains of M. kansasii sho...

journal_title：Journal of clinical microbiology

authors： Papa F,Riviere M,Fournié JJ,Puzo G,David H

更新日期：1987-12-01 00:00:00

abstract：:A radial immunodiffusion (RID) test employing a polysaccharide antigen (poly B) was compared with tests currently used in the diagnosis of bovine brucellosis. Over 1,000 sera from vaccinated and infected cattle, all of which had been examined bacteriologically, were used to determine the sensitivity and specificity of...

journal_title：Journal of clinical microbiology

authors： Jones LM,Berman DT,Moreno E,Deyoe BL,Gilsdorf MJ,Huber JD,Nicoletti P

更新日期：1980-12-01 00:00:00

abstract：:Two types of commercially available microdilution trays were inoculated with log-phase cultures, stationary-phase cultures, and direct suspensions of 18- to 24-h colonies, with essentially comparable results. Direct pick-up of colonies was also tested with two Prompt inoculation systems (3M Co.); they were found to be...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE,Hawkinson RW

更新日期：1983-09-01 00:00:00

abstract：:A total of 49 adenovirus type 3 (Ad3) strains from patients with epidemic keratoconjunctivitis at an eye clinic in Sapporo, Japan, from 1983 to 1986 were classified into either genome type Ad3f (26 strains) or Ad3g (23 strains). By the use of Hinf1 and TaqI restriction endonucleases, they were classified into 11 and 3...

journal_title：Journal of clinical microbiology

authors： Itakura S,Aoki K,Sawada H,Shinagawa M

更新日期：1990-10-01 00:00:00

abstract：:The results of anaerobic susceptibility tests performed with inocula prepared directly from agar isolation media and from overnight broth cultures were compared. Altogether, 93.0% of the results with these two inoculum preparations were within one twofold dilution, including 92.5 and 95.6% of the results with Clostrid...

journal_title：Journal of clinical microbiology

authors： Murray PR,Niles AC

更新日期：1983-09-01 00:00:00

abstract：:In an initial experiment, culture-grown Borrelia burgdorferi was added to freshly collected uninfected human blood. This in vitro study demonstrated that more spirochetes were distributed into the plasma than into the serum fraction. In a subsequent clinical study, B. burgdorferi was recovered from plasma cultures of ...

journal_title：Journal of clinical microbiology

authors： Wormser GP,Bittker S,Cooper D,Nowakowski J,Nadelman RB,Pavia C

更新日期：2000-04-01 00:00:00

abstract：:Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by thes...

journal_title：Journal of clinical microbiology

authors： Babady NE,Buckwalter SP,Hall L,Le Febre KM,Binnicker MJ,Wengenack NL

更新日期：2011-09-01 00:00:00

abstract：:Five serological assays were evaluated for the diagnosis of brucellosis in goats: the rose bengal test (RBT), complement fixation test (CFT), radial immunodiffusion (RID) with Brucella and Yersinia enterocolitica O:9 polysaccharides, counterimmunoelectrophoresis (CIEP) with cytosol, and enzyme-linked immunosorbent ass...

journal_title：Journal of clinical microbiology

authors： Díaz-Aparicio E,Marín C,Alonso-Urmeneta B,Aragón V,Pérez-Ortiz S,Pardo M,Blasco JM,Díaz R,Moriyón I

更新日期：1994-05-01 00:00:00

abstract：:Between 1998 and 2001, tissues from four captive white-tailed deer were observed to have histologic lesions of systemic lymphocytic vasculitis. These lesions suggested malignant catarrhal fever, although epizootic hemorrhagic disease and bluetongue were included in the differential diagnosis. Initial diagnostic effort...

journal_title：Journal of clinical microbiology

authors： Kleiboeker SB,Miller MA,Schommer SK,Ramos-Vara JA,Boucher M,Turnquist SE

更新日期：2002-04-01 00:00:00

abstract：:A multiplexed biomarker bundle consisting of nine different inflammation markers was evaluated regarding their diagnostic and prognostic performances in 159 adult systemic inflammatory response syndrome (SIRS) patients enrolled at the emergency department. Fibronectin, interleukin-8 (IL-8), biotin, and neutrophil gela...

journal_title：Journal of clinical microbiology

authors： Reichsoellner M,Raggam RB,Wagner J,Krause R,Hoenigl M

更新日期：2014-11-01 00:00:00

abstract：:The CO2, O2, and pH levels of commercially available blood culture bottles with tryptic soy broth medium from five different manufacturers were compared. Ranges of 1.3 to 6.9% for CO2, 1.1 to 6.0% for O2, and pH 6.94 to 7.26 were found. Different venting procedures revealed that blood culture bottles from which the ru...

journal_title：Journal of clinical microbiology

authors： Welch WD,Porschen RK,Zarifi SZ

更新日期：1984-11-01 00:00:00

abstract：:Sheep and guinea pigs were immunized with cellular and extracellular antigen from Legionella pneumophila bacteria. After immunization, the animals developed immunoglobulin G titers against the immunizing agent. The same sera were also tested in a Mycoplasma pneumoniae complement fixation test. All the preimmunization ...

journal_title：Journal of clinical microbiology

authors： Berdal BP,Eng J

更新日期：1982-11-01 00:00:00