Evaluation of serological tests for diagnosis of Brucella melitensis infection of goats.

abstract：:The prevalence of coinfection of Campylobacter species in dogs was determined using four isolation methods. In 26% of the positive-testing stools, multiple Campylobacter species were identified. The use of multiple isolation methods as well as the time lapse between sampling and processing are important for detection ...

journal_title：Journal of clinical microbiology

authors： Koene MG,Houwers DJ,Dijkstra JR,Duim B,Wagenaar JA

更新日期：2004-02-01 00:00:00

abstract：:Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuc...

journal_title：Journal of clinical microbiology

authors： Conville PS,Fischer SH,Cartwright CP,Witebsky FG

更新日期：2000-01-01 00:00:00

abstract：:Due to the inadequate automation in the amplification and sequencing procedures, the use of 16S rRNA gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. In this study, using conventional full-sequence 16...

journal_title：Journal of clinical microbiology

authors： Woo PC,Ng KH,Lau SK,Yip KT,Fung AM,Leung KW,Tam DM,Que TL,Yuen KY

更新日期：2003-05-01 00:00:00

abstract：:Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. ...

journal_title：Journal of clinical microbiology

authors： Arko RJ,Wong KH,Peacock WL

更新日期：1979-04-01 00:00:00

abstract：:Direct enzyme-linked immunosorbent assay methods offer several advantages in assessing past or recent exposure to cytomegalovirus (CMV) infection, but there persist many pitfalls in the use of these methods for determining specific immunoglobulin M (IgM). The efficiency of absorption of sera by IgG-coated latex beads,...

journal_title：Journal of clinical microbiology

authors： Joassin L,Reginster M

更新日期：1986-03-01 00:00:00

abstract：:Twenty (1.4%) of 1,421 adult Ixodes pacificus ticks and 2 (20%) of 10 adult Ixodes neotomae ticks collected in five counties of northern California were found to contain spirochetes by direct immunofluorescence examination of their tissues with a polyvalent conjugate. Borreliae isolated from the tissues of nine of the...

journal_title：Journal of clinical microbiology

authors： Lane RS,Pascocello JA

更新日期：1989-10-01 00:00:00

abstract：:Performance in proficiency testing (PT) schemes is an objective measure of a laboratory's best performance. We examined the performance of participants in two parasitology PT schemes in South Africa from 2004 through 2010. The average rates of acceptable scores over the period were 58% and 66% for the stool and blood ...

journal_title：Journal of clinical microbiology

authors： Poonsamy B,Dini L,Frean J

更新日期：2012-10-01 00:00:00

abstract：:Because of their biofilm-forming capacity, invasive Staphylococcus epidermidis isolates, which cause the majority of nosocomial catheter-related bloodstream infections (BSIs), are thought to be selected at the time of catheter insertion from a population of less virulent commensal strains. This fact allows the predict...

journal_title：Journal of clinical microbiology

authors： Rohde H,Kalitzky M,Kröger N,Scherpe S,Horstkotte MA,Knobloch JK,Zander AR,Mack D

更新日期：2004-12-01 00:00:00

abstract：:We developed an accurate nonradioactive colony hybridization assay (NCHA) using a digoxigenin-labeled polynucleotide probe and an antidigoxigenin alkaline phosphatase conjugate for the identification of enterotoxigenic Escherichia coli (ETEC) harboring genes for colonization factor antigen I (CFA/I), coli surface anti...

journal_title：Journal of clinical microbiology

authors： Sommerfelt H,Grewal HM,Gaastra W,Svennerholm AM,Bhan MK

更新日期：1992-07-01 00:00:00

abstract：:Members of the genus Exophiala are often difficult to identify to the species level because of their variable morphological appearances. This paper describes the methods used to identify Exophiala mesophila and provides salient differential features for distinguishing other mesophilic members of the genus. ...

journal_title：Journal of clinical microbiology

authors： Porteous NB,Grooters AM,Redding SW,Thompson EH,Rinaldi MG,De Hoog GS,Sutton DA

更新日期：2003-08-01 00:00:00

abstract：:We assessed the genetic and the antigenic variability within the env gene of peripheral blood human immunodeficiency virus (HIV) type 1 (HIV-1) group O populations during the natural course of a female heterosexual infection. Our data revealed the existence of a significant increase in amino acid sequence variability ...

journal_title：Journal of clinical microbiology

authors： Andréoletti L,Réveil B,Moret H,Brodard V,Philbert F,Tabary T,Cohen JH

更新日期：2007-04-01 00:00:00

abstract：:An examination of 23 vancomycin-resistant, streptococcuslike isolates of clinical origin revealed a group of 14 to be related to Leuconostoc lactis (type strain, CIP 102422) on the basis of chemotaxonomic studies. These isolates were initially shown to be atypical by classical biochemical tests. However, they were cha...

journal_title：Journal of clinical microbiology

authors： Barreau C,Wagener G

更新日期：1990-08-01 00:00:00

abstract：:The DNA relatedness and restriction fragment length polymorphism patterns of whole-cell DNA and the general phenotypic properties of 14 isolates of the form species Candida parapsilosis representing three diverse genetic groups were compared. The data confirm the unrelatedness of the three groups at the species level....

journal_title：Journal of clinical microbiology

authors： Roy B,Meyer SA

更新日期：1998-01-01 00:00:00

abstract：:Acinetobacter isolates associated with casualties from the Iraq conflict from the United States were compared with those from the United Kingdom by pulsed-field gel electrophoresis and integron analysis. Representatives of the main outbreak strain associated with casualties from both countries were indistinguishable i...

journal_title：Journal of clinical microbiology

authors： Turton JF,Kaufmann ME,Gill MJ,Pike R,Scott PT,Fishbain J,Craft D,Deye G,Riddell S,Lindler LE,Pitt TL

更新日期：2006-07-01 00:00:00

abstract：:A simple, rapid procedure to separate Pneumocystis carinii obtained by lavage of lungs of steroid-treated rats from rat leukocytes is described. Commercially available monoclonal antibody to rat common leukocyte determinants is used to sediment the leukocytes, resulting in supernatant fluid containing P. carinii cells...

journal_title：Journal of clinical microbiology

authors： Pesanti EL,McCarron BA

更新日期：1988-08-01 00:00:00

abstract：:A TaqMan PCR was established for identification and quantitation of members of the Ehrlichia phagocytophila group in experimentally infected cows and in Ixodes ricinus ticks. The TaqMan PCR identified a 106-bp section of the 16S rRNA gene by use of a specific fluorogenic probe and two primers. This technique was speci...

journal_title：Journal of clinical microbiology

authors： Pusterla N,Huder JB,Leutenegger CM,Braun U,Madigan JE,Lutz H

更新日期：1999-05-01 00:00:00

abstract：:Early diagnosis of Neisseria gonorrhoeae infections is important with regard to patients' health and infectivity. We report the development of a specific and sensitive TaqMan assay for the detection of N. gonorrhoeae in clinical samples. The target sequence is a 76-bp fragment of the 5' untranslated region of the opa ...

journal_title：Journal of clinical microbiology

authors： Geraats-Peters CW,Brouwers M,Schneeberger PM,van der Zanden AG,Bruisten SM,Weers-Pothoff G,Boel CH,van den Brule AJ,Harmsen HG,Hermans MH

更新日期：2005-11-01 00:00:00

abstract：:Ten clinical yeast isolates submitted to the Centers for Disease Control from diverse geographic areas were identified as Candida ciferrii and Candida chiropterorum. The association of C. ciferrii with clinical specimens, particularly its repeated isolation from a case of onychomycosis, suggests that this species may ...

journal_title：Journal of clinical microbiology

authors： Furman RM,Ahearn DG

更新日期：1983-11-01 00:00:00

abstract：:Human metapneumovirus (HMPV) was the unique viral pathogen detected by a real-time reverse transcriptase PCR (RT-PCR) assay in 6 (6.4%) of 94 consecutive French children hospitalized for acute bronchiolitis from September 2001 to June 2002. This virus was identified as the third etiological cause of bronchiolitis, aft...

journal_title：Journal of clinical microbiology

authors： Bouscambert-Duchamp M,Lina B,Trompette A,Moret H,Motte J,Andréoletti L

更新日期：2005-03-01 00:00:00

abstract：:The impact of the viability of Trichomonas vaginalis in urine on wet mount, culture, and PCR methods was assessed. To minimize the chance of false-negative results, urine specimens should be processed within 30 min of specimen collection and maintained at 37 degrees C, since temperature appears to affect the viability...

journal_title：Journal of clinical microbiology

authors： Shafir SC,Sorvillo FJ

更新日期：2006-10-01 00:00:00

abstract：:The value of immunoglobulin M (IgM) detection in the early diagnosis of influenza A was examined in a prospective study during an outbreak of influenza A/Philippines/2/82 (H3N2) virus infection in February and March 1986. The IgM response was investigated in sera from 64 adults with influenzalike symptoms; we found a ...

journal_title：Journal of clinical microbiology

authors： Vikerfors T,Lindegren G,Grandien M,van der Logt J

更新日期：1989-03-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek's disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did t...

journal_title：Journal of clinical microbiology

authors： Angamuthu R,Baskaran S,Gopal DR,Devarajan J,Kathaperumal K

更新日期：2012-03-01 00:00:00

abstract：:Rapid identification and differentiation of orthopoxviruses by PCR were achieved with primers based on genome sequences encoding the hemagglutinin (HA) protein, an infected-cell membrane antigen that distinguishes orthopoxviruses from other poxvirus genera. The initial identification step used a primer pair of consens...

journal_title：Journal of clinical microbiology

authors： Ropp SL,Jin Q,Knight JC,Massung RF,Esposito JJ

更新日期：1995-08-01 00:00:00

abstract：:We evaluated the performance and the cost of toxigenic culture using a commercial chromogenic medium (CDIF) for 538 stool specimens. Compared with real-time PCR, this method was found to detect an additional 9% of positive specimens and result in 61% reduction in material costs, with a trade-off increase in turnaround...

journal_title：Journal of clinical microbiology

authors： Luk S,To WK,Ng TK,Hui WT,Lee WK,Lau F,Ching AM

更新日期：2014-02-01 00:00:00

abstract：:Yersinia enterocolitica is known to be associated with gastroenteritis in children and Reiter's syndrome in adults, but it has only rarely been the cause of primary soft tissue infections. A patient with diabetes mellitus developed a calf abscess, from which Y. enterocolitica was isolated in pure culture. Incision, dr...

journal_title：Journal of clinical microbiology

authors： Brennessel DJ,Robbins N,Hindman S

更新日期：1984-08-01 00:00:00

abstract：:MICs and minimal bactericidal concentrations (MBCs) were evaluated for the four antibiotics azithromycin, amoxicillin, ceftriaxone, and doxycycline against the three main genospecies of Borrelia burgdorferi sensu lato. In MBC testing, statistically significant differences between the genospecies could be found in 7 ou...

journal_title：Journal of clinical microbiology

authors： Sicklinger M,Wienecke R,Neubert U

更新日期：2003-04-01 00:00:00

abstract：:A total of 192 hybridomas were developed from mice immunized with Rickettsia japonica, a newly identified spotted fever group rickettsia pathogenic for humans. Of these hybridomas, 101 were species specific, 37 were spotted fever group reactive, and the other 54 were also reactive with one or more of the other pathoge...

journal_title：Journal of clinical microbiology

authors： Uchiyama T,Uchida T,Walker DH

更新日期：1990-06-01 00:00:00

abstract：:Serogrouping of Bacteroides nodosus is based on antigenic differences in fimbriae of the different New Zealand prototype strains. Because of the time needed to isolate and grow pure cultures of B. nodosus and the difficulty in distinguishing between different serogroups because of cross-agglutination, a new DNA-based ...

journal_title：Journal of clinical microbiology

authors： John GH,Carlson JO,Kimberling CV,Ellis RP

更新日期：1990-11-01 00:00:00

abstract：:Escherichia hermannii was isolated in pure culture from a patient with acute purulent conjunctivitis after a minor ocular injury. This is the first report of E. hermannii isolated as the sole pathogen from an infected site without prior antibiotic exposure, confirming the pathogenic potential of the microorganism. ...

journal_title：Journal of clinical microbiology

authors： Poulou A,Dimitroulia E,Markou F,Tsakris A

更新日期：2008-11-01 00:00:00

abstract：:Enteroviral meningoencephalitis was diagnosed in a patient with an immunodeficiency syndrome acquired after treatment with rituximab for a relapsed primary B-cell lymphoma. A second meningoencephalitic episode was diagnosed 6 months later and was successfully treated with a combination of immunoglobulins and pleconari...

journal_title：Journal of clinical microbiology

authors： Archimbaud C,Bailly JL,Chambon M,Tournilhac O,Travade P,Peigue-Lafeuille H

更新日期：2003-10-01 00:00:00