Polymerase chain reaction amplification of the constant and variable regions of the Bacteroides nodosus fimbrial gene.

abstract：:A diagnostic test for feline infectious peritonitis virus (FIPV) infection based on a nested PCR (nPCR) assay was developed and tested with FIPV, feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV) and clinical fluid samples from cats with effusive feline infecti...

journal_title：Journal of clinical microbiology

authors： Gamble DA,Lobbiani A,Gramegna M,Moore LE,Colucci G

更新日期：1997-03-01 00:00:00

abstract：:Evaluation of previously described chemically defined media for the growth of Legionella pneumophila showed that these media supported poor growth of several strains of L. pneumophila and did not support growth of certain of the Legionella species described later. Growth was stimulated by the dialysate from yeast extr...

journal_title：Journal of clinical microbiology

authors： Pine L,Franzus MJ,Malcolm GB

更新日期：1986-01-01 00:00:00

abstract：:Cefaclor is less stable than most cephalosporins in media at 35 degrees C. We demonstrated that the activity of cefaclor in Mueller-Hinton agar diminishes continuously at 4 degrees C, resulting in a loss of two-thirds of the activity within 21 days. We recommend that agar dilution plates for this cephalosporin be prep...

journal_title：Journal of clinical microbiology

authors： Surprenant AM,Preston DA

更新日期：1985-01-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for the detection and quantitation of human immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica is described. Formalinized or heat-treated bacteria were adsorbed onto specially designed microcuvettes, and antibodies were allowed to attach to the antigen-...

journal_title：Journal of clinical microbiology

authors： Granfors K

更新日期：1979-03-01 00:00:00

abstract：:This study reports the first isolation of Candida dubliniensis from North American Indians. Of 39 healthy human hosts sampled, two had C. dubliniensis. Genotypic analysis identified polymorphisms in these strains and differences from two reference strains. Our results suggest that yeast populations from indigenous com...

journal_title：Journal of clinical microbiology

authors： Montour L,Tey R,Xu J

更新日期：2003-07-01 00:00:00

abstract：:The API NeIdent system (Analytab Products, Plainview, N.Y.) was evaluated for identifying Neisseria spp. and Branhamella catarrhalis commonly isolated from clinical specimens. The system identified 90% of 303 Neisseria gonorrhoeae isolates, 71% of 113 Neisseria meningitidis isolates, and 63% of 16 Neisseria lactamica ...

journal_title：Journal of clinical microbiology

authors： Janda WM,Morello JA,Bohnhoff M

更新日期：1984-03-01 00:00:00

abstract：:The prevalence of class D extended-spectrum oxacillinases (ES-OXAs) in ceftazidime-resistant strains of Pseudomonas aeruginosa is often underestimated by double-disk synergy tests (DDST) using clavulanate. A DDST with a customized distance between a disk of ceftazidime or cefepime and inhibitors (clavulanate and imipe...

journal_title：Journal of clinical microbiology

authors： Hocquet D,Dehecq B,Bertrand X,Plésiat P

更新日期：2011-06-01 00:00:00

abstract：:Ewingella americana was isolated from two blood cultures from a 75-year-old male after cholecystectomy. The characteristics of this strain are compared with the reported biochemical characteristics of 44 American strains. Previously described infections and pseudoinfections with E. americana are reviewed. ...

journal_title：Journal of clinical microbiology

authors： Devreese K,Claeys G,Verschraegen G

更新日期：1992-10-01 00:00:00

abstract：:A procedure for detecting pneumococcal capsular polysaccharides in immune complexes is described. Separation of antigen from immune complexes is achieved by electrophoresis at 56 degrees C. ...

journal_title：Journal of clinical microbiology

authors： Coonrod JD,Leach RP

更新日期：1978-08-01 00:00:00

abstract：:In West African countries such as Ghana, efficient human immunodeficiency virus (HIV) testing is a priority in the fight against AIDS. A new immunochromatographic rapid test, Determine HIV-1/2 (Abbott Diagnostics, North Chicago, Ill.), that detects antibodies against HIV type 1 (HIV-1) and/or HIV-2 was evaluated using...

journal_title：Journal of clinical microbiology

authors： Aidoo S,Ampofo WK,Brandful JA,Nuvor SV,Ansah JK,Nii-Trebi N,Barnor JS,Apeagyei F,Sata T,Ofori-Adjei D,Ishikawa K

更新日期：2001-07-01 00:00:00

abstract：:The first FDA-approved multiplex PCR panel for a large number of respiratory pathogens was introduced in 2008. Since then, other PCR panels for detection of several respiratory and gastrointestinal pathogens have been approved by the FDA and are commercially available, and more such panels are likely to become availab...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,McAdam AJ

更新日期：2015-10-01 00:00:00

abstract：:To investigate whether both tissue culture and PCR on a sequence from the repetitive rDNA could contribute to the diagnosis of toxoplasmosis, blood samples and, if they were available, cerebrospinal fluid (CSF) and aqueous humor samples from 72 human immunodeficiency virus-seropositive patients with suspected toxoplas...

journal_title：Journal of clinical microbiology

authors： Dupon M,Cazenave J,Pellegrin JL,Ragnaud JM,Cheyrou A,Fischer I,Leng B,Lacut JY

更新日期：1995-09-01 00:00:00

abstract：:The production and characterization of five monoclonal antibodies to Mycobacterium tuberculosis are described. Specificity of the monoclonal antibodies was tested against other mycobacterial species by an enzyme-linked immunosorbent assay and immunoblots. HGT 3a, an immunoglobulin M (IgM) antibody, recognizes a molecu...

journal_title：Journal of clinical microbiology

authors： Kadival GV,Chaparas SD

更新日期：1987-01-01 00:00:00

abstract：:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of an ongoing pandemic that has infected over 36 million and killed over 1 million people. Informed implementation of government public health policies depends on accurate data on SARS-CoV-2 immunity at a population scale. We hypothesized that d...

journal_title：Journal of clinical microbiology

authors： Pisanic N,Randad PR,Kruczynski K,Manabe YC,Thomas DL,Pekosz A,Klein SL,Betenbaugh MJ,Clarke WA,Laeyendecker O,Caturegli PP,Larman HB,Detrick B,Fairley JK,Sherman AC,Rouphael N,Edupuganti S,Granger DA,Granger SW,Coll

更新日期：2020-12-17 00:00:00

abstract：:Current nucleic acid amplification methods to detect Mycobacterium tuberculosis are complex, labor-intensive, and technically challenging. We developed and performed the first analysis of the Cepheid Gene Xpert System's MTB/RIF assay, an integrated hands-free sputum-processing and real-time PCR system with rapid on-de...

journal_title：Journal of clinical microbiology

authors： Helb D,Jones M,Story E,Boehme C,Wallace E,Ho K,Kop J,Owens MR,Rodgers R,Banada P,Safi H,Blakemore R,Lan NT,Jones-López EC,Levi M,Burday M,Ayakaka I,Mugerwa RD,McMillan B,Winn-Deen E,Christel L,Dailey P,Perkins

更新日期：2010-01-01 00:00:00

abstract：:We report the first case of maxillary sinusitis caused by Actinomucor elegans in an 11-year-old patient. Histopathological and mycological examinations of surgical maxillary sinuses samples showed coenocytic hyphae characteristic of mucoraceous fungi. The fungi recovered had stolons and rhizoids, nonapophyseal and glo...

journal_title：Journal of clinical microbiology

authors： Davel G,Featherston P,Fernández A,Abrantes R,Canteros C,Rodero L,Sztern C,Perrotta D

更新日期：2001-02-01 00:00:00

abstract：:Little information is available about the genetic variability of Leishmania populations and the possible correlations with ecoepidemiological features of leishmaniases. The present study was carried out in French Guiana, a country where cutaneous leishmaniases (CL) are endemic over the whole territory. The genetic pol...

journal_title：Journal of clinical microbiology

authors： Rotureau B,Ravel C,Nacher M,Couppié P,Curtet I,Dedet JP,Carme B

更新日期：2006-02-01 00:00:00

abstract：:Diagnostic use of a direct fluorescent-antibody test for detection of Campylobacter jejuni and C. coli in human fecal specimens (n = 497) was compared with detection by culturing (specificity, 99.7%; sensitivity, 40%). Conjugates were prepared from immunoglobulin G antibody against 22 Lior C. jejuni and C. coli refere...

journal_title：Journal of clinical microbiology

authors： Hodge DS,Prescott JF,Shewen PE

更新日期：1986-11-01 00:00:00

abstract：:Twenty-one reference strains and 88 clinical isolates of Aerococcus, Enterococcus, and Streptococcus species were tested for reactions in the Rapid Strep (RS) and modified conventional tests. We conclude that some but not all of the tests in the RS system could be used to substitute for conventional tests. RS tests fo...

journal_title：Journal of clinical microbiology

authors： Fertally SS,Facklam R

更新日期：1987-10-01 00:00:00

abstract：:This study evaluated the feasibility of a molecular strategy based on identification of Mycobacterium tuberculosis by IS6110 PCR or Cobas Amplicor PCR, and rpoB PCR-DNA sequencing of the 81-bp rifampin resistance determining region (RRDR) for direct detection of rifampin resistance in respiratory specimens. A collecti...

journal_title：Journal of clinical microbiology

authors： Yam WC,Tam CM,Leung CC,Tong HL,Chan KH,Leung ET,Wong KC,Yew WW,Seto WH,Yuen KY,Ho PL

更新日期：2004-10-01 00:00:00

abstract：:During an outbreak of diarrhea in a general hospital in 1992, 166 Clostridium difficile isolates from 102 patients were typed by restriction enzyme analysis (REA), arbitrarily primed PCR (AP-PCR), and protein profile analysis (PP) techniques. A total of 18 types and 5 subtypes were identified by REA, 32 types were ide...

journal_title：Journal of clinical microbiology

authors： Rafferty ME,Baltch AL,Smith RP,Bopp LH,Rheal C,Tenover FC,Killgore GE,Lyerly DM,Wilkins TD,Schoonmaker DJ,Hannett GE,Shayegani M

更新日期：1998-10-01 00:00:00

abstract：:Multilocus variable-number tandem-repeat analysis (MLVA) for seven genomic loci was developed for Enterococcus faecalis. MLVA and pulsed-field gel electrophoresis (PFGE) resulted in 37 and 31 genotypes among 83 strains, respectively. Both typing schemes were highly concordant (90.4%). MLVA is an excellent alternative ...

journal_title：Journal of clinical microbiology

authors： Titze-de-Almeida R,Willems RJ,Top J,Rodrigues IP,Ferreira RF 2nd,Boelens H,Brandileone MC,Zanella RC,Felipe MS,van Belkum A

更新日期：2004-10-01 00:00:00

abstract：:Knowledge of polyomavirus BK (BKV) genomic diversity has greatly expanded. The implications of BKV DNA sequence variation for the performance of molecular diagnostic assays is not well studied. We analyzed 184 publically available VP-1 sequences encompassing the BKV genomic region targeted by an in-house quantitative ...

journal_title：Journal of clinical microbiology

authors： Randhawa P,Kant J,Shapiro R,Tan H,Basu A,Luo C

更新日期：2011-12-01 00:00:00

abstract：:To select a method for detecting methicillin-resistant Staphylococcus aureus (MRSA) in nasal swabs, we compared BD GeneOhm MRSA PCR and various culture media (mannitol salt agar with cefoxitin, MRSASelect, CHROMagar MRSA, and Spectra MRSA). While PCR detection of MRSA was more rapid, MRSASelect and Spectra MRSA demons...

journal_title：Journal of clinical microbiology

authors： Bischof LJ,Lapsley L,Fontecchio K,Jacosalem D,Young C,Hankerd R,Newton DW

更新日期：2009-07-01 00:00:00

abstract：:Several unusual fatty acids characterized strains of Francisella tularensis. Long-chain (C20-C26) acids and the hydroxy acids 2-hydroxy-decanoate, 3-hydroxy-hexadecanoate, and 3-hydroxy-octadecanoate appeared to be of special diagnostic value. ...

journal_title：Journal of clinical microbiology

authors： Jantzen E,Berdal BP,Omland T

更新日期：1979-12-01 00:00:00

abstract：:Staphylococcus lugdunensis is a coagulase-negative staphylococcus that has several similarities to Staphylococcus aureus. S. lugdunensis is increasingly being recognized as a cause of prosthetic joint infection (PJI). The goal of the present retrospective cohort study was to determine the laboratory and clinical chara...

journal_title：Journal of clinical microbiology

authors： Shah NB,Osmon DR,Fadel H,Patel R,Kohner PC,Steckelberg JM,Mabry T,Berbari EF

更新日期：2010-05-01 00:00:00

abstract：:We used genotypic and phenotypic assays to estimate the frequency of X4/DM viruses in 131 patients infected with non-subtype-B viruses at the time of primary HIV-1 infection (PHI). All patients were enrolled in the French PRIMO Cohort from 1996 to 2007. Most strains belonged to CRF02_AG (51.1%) and subtype A (14.5%). ...

journal_title：Journal of clinical microbiology

authors： Frange P,Chaix ML,Raymond S,Galimand J,Deveau C,Meyer L,Goujard C,Rouzioux C,Izopet J,French ANRS CO 06 PRIMO Cohort Study Group.

更新日期：2010-10-01 00:00:00

abstract：:Procedures for differentiation between wild and vaccine-derived strains of poliovirus are required, particularly in countries where wild and vaccine-related strains coexist. For this differentiation, we tested the method of Inouye and Hondo (S. Inouye and R. Hondo, Arch. Virol. 129:311-316, 1993) for discrimination of...

journal_title：Journal of clinical microbiology

authors： Takeda N,Sakae K,Agboatwalla M,Isomura S,Hondo R,Inouye S

更新日期：1994-01-01 00:00:00

abstract：:We compared the results of Gram staining and culture of cerebrospinal fluid to results obtained with a rapid PCR assay for the diagnosis of meningococcal meningitis in 281 cases of suspected bacterial meningitis. PCR had a sensitivity of 97% compared to a sensitivity of 55% for culture, and the PCR specificity was 99....

journal_title：Journal of clinical microbiology

authors： Richardson DC,Louie L,Louie M,Simor AE

更新日期：2003-08-01 00:00:00

abstract：:Colistin-nalidixic acid agar, although recently recommended as a replacement for blood agar for primary plating of urine specimens ( Fung et al., J. Clin. Microbiol. 16:632-636, 1982), has also been reported to suppress the growth of some strains of staphylococci that are susceptible to colistin (polymyxin E). The sus...

journal_title：Journal of clinical microbiology

authors： Fung JC,McKinley G,Tyburski MB,Berman M,Goldstein J

更新日期：1984-05-01 00:00:00