Comparison of chromogenic media to BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR for detection of MRSA in nasal swabs.

abstract：:An automated enzymatic method was developed for the measurement of D-arabinitol in human serum. The assay is based on a novel, highly specific D-arabinitol dehydrogenase from Candida tropicalis. This enzyme catalyzes the oxidation of D-arabinitol to D-ribulose and the concomitant reduction of NAD+ to NADH. The NADH pr...

journal_title：Journal of clinical microbiology

authors： Switchenko AC,Miyada CG,Goodman TC,Walsh TJ,Wong B,Becker MJ,Ullman EF

更新日期：1994-01-01 00:00:00

abstract：:Cag pathogenicity island-containing Helicobacter pylori (type I) induces signal transduction pathways resulting in tyrosine phosphorylation of proteins adjacent to the site of bacterial adhesion on host gastric epithelial cells. Conventional block PCR-restriction fragment length polymorphism (RFLP) and real-time Light...

journal_title：Journal of clinical microbiology

authors： Owen RJ,Sharp SI,Chisholm SA,Rijpkema S

更新日期：2003-07-01 00:00:00

abstract：:Six rapid agglutination tests for identification of Staphylococcus aureus were evaluated by using 62 strains of S. aureus, 63 strains of S. saprophyticus, and 67 strains of other coagulase-negative staphylococci. S. saprophyticus was responsible for 19 of 26 false-positive results and 20 uninterpretable reactions. Thu...

journal_title：Journal of clinical microbiology

authors： Gregson DB,Low DE,Skulnick M,Simor AE

更新日期：1988-07-01 00:00:00

abstract：:Increasing frequencies of community-acquired methicillin-resistant Staphylococcus aureus (MRSA) strain isolation have been reported from many countries. The overall prevalence of MRSA in Norway is still very low. MRSA isolates (n = 67) detected between 1995 and 2003 in northern Norway were analyzed by pulsed-field gel...

journal_title：Journal of clinical microbiology

authors： Hanssen AM,Fossum A,Mikalsen J,Halvorsen DS,Bukholm G,Sollid JU

更新日期：2005-05-01 00:00:00

abstract：:A novel PCR detection assay that amplifies the Helicobacter pylori-specific vacuolating cytotoxin gene (vacA) and thus enables rapid diagnosis of infection is described. Additionally, a real-time probe hybridization melting point analysis assay to detect all three mutations in the 23S rRNA gene associated with clarith...

journal_title：Journal of clinical microbiology

authors： Chisholm SA,Owen RJ,Teare EL,Saverymuttu S

更新日期：2001-04-01 00:00:00

abstract：:Cellulosimicrobium funkei is a rare, opportunistic pathogen. We describe a case of bacteremia and possibly prosthetic valve endocarditis by this organism in a nonimmunocompromised patient. Useful phenotypic tests for differentiating C. funkei from Cellulosimicrobium cellulans and Cellulosimicrobium terreum include mot...

journal_title：Journal of clinical microbiology

authors： Petkar H,Li A,Bunce N,Duffy K,Malnick H,Shah JJ

更新日期：2011-03-01 00:00:00

abstract：:An international program of surveillance of bloodstream infections (BSIs) in the United States, Canada, and South America between January and December 1997 detected 306 episodes of candidemia in 34 medical centers (22 in the United States, 6 in Canada, and 6 in South America). Eighty percent of the BSIs were nosocomia...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Jones RN,Doern GV,Sader HS,Hollis RJ,Messer SA

更新日期：1998-07-01 00:00:00

abstract：:Reference strains of serotypes 1 to 12 of Actinobacillus (Haemophilus) pleuropneumoniae were cultured in Eagle minimal essential medium with 10% Serum Plus. Culture supernatants were examined for cytotoxicity to alveolar macrophages and for the ability to hemolyze sheep erythrocytes. All strains except the reference s...

journal_title：Journal of clinical microbiology

authors： Kamp EM,van Leengoed LA

更新日期：1989-06-01 00:00:00

abstract：:Branched DNA (bDNA) assays to quantify human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) consist of three distinct steps, including sample processing, hybridization, and detection, and utilize the System 340 platform for plate incubation and washing. Sample processing differs: HIV-1 from 1 ml of ...

journal_title：Journal of clinical microbiology

authors： Elbeik T,Markowitz N,Nassos P,Kumar U,Beringer S,Haller B,Ng V

更新日期：2004-07-01 00:00:00

abstract：:Two bacteriologic systems for detecting bacteria in blood were compared; the automated radiometric BACTEC and the conventional method used in our laboratory for many years. BACTEC consisted of two bottles with 30 ml and the conventional method with 50 ml of media for aerobes and anaerobes. The BACTEC bottles were inoc...

journal_title：Journal of clinical microbiology

authors： Thiemke WA,Wicher K

更新日期：1975-03-01 00:00:00

abstract：:The identification and elimination of persistently infected (PI) cattle are the most effective measures for controlling bovine pestiviruses, including bovine viral diarrhea virus (BVDV) and the emerging HoBi-like viruses. Here, colostrum-deprived calves persistently infected with HoBi-like pestivirus (HoBi-like PI cal...

journal_title：Journal of clinical microbiology

authors： Bauermann FV,Falkenberg SM,Vander Ley B,Decaro N,Brodersen BW,Harmon A,Hessman B,Flores EF,Ridpath JF

更新日期：2014-11-01 00:00:00

abstract：:Hepatitis E virus (HEV) is responsible for a majority of sporadic and epidemic viral hepatitides in India and other developing countries. Even though the genomes of four geographically distinct strains of HEV have been cloned and sequenced, the Indian strain of HEV remains largely uncharacterized. We have cloned and s...

journal_title：Journal of clinical microbiology

authors： Panda SK,Nanda SK,Zafrullah M,Ansari IH,Ozdener MH,Jameel S

更新日期：1995-10-01 00:00:00

abstract：:Real-time PCR (rt-PCR) is an important diagnostic tool for the identification of Bordetella pertussis, Bordetella holmesii, and Bordetella parapertussis. Most U.S. public health laboratories (USPHLs) target IS481, present in 218 to 238 copies in the B. pertussis genome and 32 to 65 copies in B. holmesii. The CDC devel...

journal_title：Journal of clinical microbiology

authors： Williams MM,Taylor TH Jr,Warshauer DM,Martin MD,Valley AM,Tondella ML

更新日期：2015-01-01 00:00:00

abstract：:A disease similar to ulcerative colitis in humans has been identified in cotton-top tamarins (CTTs) in captivity. The clinical signs include weight loss, diarrhea, and rectal bleeding with the pathological features and biochemical abnormalities of ulcerative colitis. Approximately 25 to 40% of these animals develop co...

journal_title：Journal of clinical microbiology

authors： Saunders KE,Shen Z,Dewhirst FE,Paster BJ,Dangler CA,Fox JG

更新日期：1999-01-01 00:00:00

abstract：:Cryptococcus gattii emerged in 1999 in a distinct ecologic environment on Vancouver Island, Canada. Estimates of incubation period were derived from an analysis of travelers with discreet exposures to this region. Individual case incubation periods ranged from 2 to 11 months, with a median of 6 to 7 months. ...

journal_title：Journal of clinical microbiology

authors： MacDougall L,Fyfe M

更新日期：2006-05-01 00:00:00

abstract：:An indirect fluorescent antibody technique has been developed for the serological diagnosis of gonorrhea. The selected strain(s) of Neisseria gonorrhoeae possesses a heat-labile surface antigen (L-antigen). Sera are diluted 1:10, and an aliquot is hear inactivated at 59 C for 30 min. The treated and untreated aliquots...

journal_title：Journal of clinical microbiology

authors： Gaafar HA,D'Arcangelis DC

更新日期：1976-04-01 00:00:00

abstract：:Of 532 strains of viridans streptococci isolated from clinical specimens, 517 were identified by using a scheme based on the work of Facklam (R. R. Facklam, J. Clin. Microbiol. 5:184-201, 1977). The strains were distributed among nine of the species described by Facklam and an additional group of physiologically homog...

journal_title：Journal of clinical microbiology

authors： Ruoff KL,Kunz LJ

更新日期：1982-05-01 00:00:00

abstract：:This study estimated the regional and age- and gender-specific seroprevalences of herpes simplex virus type 1 (HSV-1) and HSV-2 in Ontario, Canada. Stored serum specimens from subjects aged 15 to 44 years, including men (n = 979), women not under prenatal care (n = 638), and women under prenatal care (n = 701) submitt...

journal_title：Journal of clinical microbiology

authors： Howard M,Sellors JW,Jang D,Robinson NJ,Fearon M,Kaczorowski J,Chernesky M

更新日期：2003-01-01 00:00:00

abstract：:A total of 137 strains of Staphylococcus species were blindly tested by four rapid serological assays, and the results were compared with those of the tube coagulase assay. For the S. aureus isolates, the Sero-STAT Staph assay gave six false-negative results, four of which were for methicillin-resistant strains. The A...

journal_title：Journal of clinical microbiology

authors： Aldridge KE,Kogos C,Sanders CV,Marier RL

更新日期：1984-05-01 00:00:00

abstract：:The specific identification of verocytotoxin (VT)-producing Escherichia coli (VTEC) requires the detection of VTs in bacterial culture filtrates or the detection of genes encoding these toxins in bacterial cells by specific DNA probes. The standard method for detecting these toxins involves a time-consuming, labor-int...

journal_title：Journal of clinical microbiology

authors： Basta M,Karmali M,Lingwood C

更新日期：1989-07-01 00:00:00

abstract：:To establish the exact pathogenic role of Mycobacterium abscessus in cystic fibrosis (CF), molecular tests are required for accurate identification. Forty-eight M. abscessus isolates from seven patients with CF were analyzed by sequence analysis for sequence variants within the hsp65 gene and the 16S-23S intergenic se...

journal_title：Journal of clinical microbiology

authors： König B,Tammer I,Sollich V,König W

更新日期：2005-07-01 00:00:00

abstract：:We retrospectively analyzed epidemiologic information associated with 22 cultures of Neisseria elongata subsp. nitroreducens (formerly CDC group M-6) submitted to the Microbial Diseases Laboratory, California Department of Health Services, Berkeley, over a 16-year period. The most common illnesses noted with this bact...

journal_title：Journal of clinical microbiology

authors： Wong JD,Janda JM

更新日期：1992-03-01 00:00:00

abstract：:A new integrated extraction and real-time PCR-based system for the detection of group B streptococci in antepartum screening samples enriched in Lim broth was compared to the CDC-recommended culture method. The BD Max GBS assay exhibited acceptable sensitivity (95%) and specificity (96.7%) compared to those of the cul...

journal_title：Journal of clinical microbiology

authors： Riedlinger J,Beqaj SH,Milish MA,Young S,Smith R,Dodd M,Hankerd RE,Lebar WD,Newton DW

更新日期：2010-11-01 00:00:00

abstract：:Candida dubliniensis is often associated with C. albicans in cultures. Easy-to-perform selective isolation procedures for these closely related species do not exist. Therefore, we evaluated previously described discriminatory phenotypic markers for C. dubliniensis. A total of 150 oral rinses from human immunodeficienc...

journal_title：Journal of clinical microbiology

authors： Tintelnot K,Haase G,Seibold M,Bergmann F,Staemmler M,Franz T,Naumann D

更新日期：2000-04-01 00:00:00

abstract：:CHROMagar Candida is a selective and differential chromogenic medium that has been shown to be useful for identification of Candida albicans, Candida krusei, Candida tropicalis, and perhaps Candida glabrata. Colony morphology and color have been well defined when CHROMagar Candida has been used to isolate yeast direct...

journal_title：Journal of clinical microbiology

authors： Horvath LL,Hospenthal DR,Murray CK,Dooley DP

更新日期：2003-06-01 00:00:00

abstract：:The first outbreak caused by vancomycin-resistant enterococci of the VanB phenotype in Poland was analyzed. It occurred in a single ward of a Warsaw hospital which is a specialized center for the treatment of hematological disorders. Between July 1999 and February 2000, 11 patients in the ward were found to be infecte...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zaleska M,Ozorowski T,Konopka L,Hryniewicz W

更新日期：2001-05-01 00:00:00

abstract：:A direct comparison of two automated blood culture systems was conducted to compare their ability to detect Candida growth. The systems evaluated were the BACTEC 9240 (Bactec) and BacT/ALERT 3D (BacT). The aerobic, anaerobic, and mycology media for each system were evaluated: Bactec Plus Aerobic/F, Plus Anaerobic/F, a...

journal_title：Journal of clinical microbiology

authors： Horvath LL,George BJ,Murray CK,Harrison LS,Hospenthal DR

更新日期：2004-01-01 00:00:00

abstract：:We developed an accurate nonradioactive colony hybridization assay (NCHA) using a digoxigenin-labeled polynucleotide probe and an antidigoxigenin alkaline phosphatase conjugate for the identification of enterotoxigenic Escherichia coli (ETEC) harboring genes for colonization factor antigen I (CFA/I), coli surface anti...

journal_title：Journal of clinical microbiology

authors： Sommerfelt H,Grewal HM,Gaastra W,Svennerholm AM,Bhan MK

更新日期：1992-07-01 00:00:00

abstract：:Sixty-one Bordetella pertussis isolates were tested blindly in two laboratories to determine their serotype nature by monoclonal antibodies using two independent methods: the standard bacterial microagglutination assay and an indirect whole-cell enzyme-linked immunosorbent assay. Both methods gave concordant results i...

journal_title：Journal of clinical microbiology

authors： Tsang RS,Sill ML,Advani A,Xing D,Newland P,Hallander H

更新日期：2005-05-01 00:00:00

abstract：:We mapped the coding single nucleotide polymorphisms in four toxin genes-exoS, exoT, exoU, and exoY-of the Pseudomonas aeruginosa type III secretion system among several clinical isolates. We then used this information to design a multiplex PCR assay based on the simultaneous amplification of fragments of these genes....

journal_title：Journal of clinical microbiology

authors： Ajayi T,Allmond LR,Sawa T,Wiener-Kronish JP

更新日期：2003-08-01 00:00:00