Simultaneous runs of the Bayer VERSANT HIV-1 version 3.0 and HCV bDNA version 3.0 quantitative assays on the system 340 platform provide reliable quantitation and improved work flow.

abstract：:Interferon gamma (IFN-γ) release assays (IGRAs) are increasingly used to test for latent tuberculosis (TB) infection. Although highly specific, IGRAs have a relatively high false-negative rate in active TB patients. A more sensitive assay is needed. IFN-γ-induced protein 10 (IP-10) is an alternative biomarker with a 1...

journal_title：Journal of clinical microbiology

authors： Blauenfeldt T,Villar-Hernández R,García-García E,Latorre I,Holm LL,Muriel-Moreno B,De Souza-Galvão ML,Millet JP,Sabriá F,Sánchez-Montalva A,Ruiz-Manzano J,Pilarte J,Jiménez MA,Centeno C,Torres C,Molina-Pinargote I,Gonzále

更新日期：2020-09-22 00:00:00

abstract：:The noninvasive diagnosis of amebic liver abscess is challenging, as most patients at the time of diagnosis do not have a concurrent intestinal infection with Entamoeba histolytica. Fecal testing for E. histolytica parasite antigen or DNA is negative in most patients. A real-time PCR assay was evaluated for detection ...

journal_title：Journal of clinical microbiology

authors： Haque R,Kabir M,Noor Z,Rahman SM,Mondal D,Alam F,Rahman I,Al Mahmood A,Ahmed N,Petri WA Jr

更新日期：2010-08-01 00:00:00

abstract：:We designed a highly sensitive and specific polymerase chain reaction assay for the detection of Epstein-Barr virus (EBV)-related sequences in B-cell DNA of EBV-seropositive healthy individuals. By using this assay, we were able to amplify at least 10 copies of a plasmid containing the BamHI-W region, which is repeate...

journal_title：Journal of clinical microbiology

authors： Wagner HJ,Bein G,Bitsch A,Kirchner H

更新日期：1992-11-01 00:00:00

abstract：:Recent molecular studies have shown Mycobacterium porcinum, recovered from cases of lymphadenitis in swine, to have complete 16S rDNA sequence identity and >70% DNA-DNA homology with human isolates within the M. fortuitum third biovariant complex. We identified 67 clinical and two environmental isolates of the M. fort...

journal_title：Journal of clinical microbiology

authors： Wallace RJ Jr,Brown-Elliott BA,Wilson RW,Mann L,Hall L,Zhang Y,Jost KC Jr,Brown JM,Kabani A,Schinsky MF,Steigerwalt AG,Crist CJ,Roberts GD,Blacklock Z,Tsukamura M,Silcox V,Turenne C

更新日期：2004-12-01 00:00:00

abstract：:Broad-spectrum analysis for pathogens in patients with respiratory tract infections is becoming more relevant as the number of potential infectious agents is still increasing. Here we describe the new multiparameter RespiFinder assay, which is based on the multiplex ligation-dependent probe amplification (MLPA) techno...

journal_title：Journal of clinical microbiology

authors： Reijans M,Dingemans G,Klaassen CH,Meis JF,Keijdener J,Mulders B,Eadie K,van Leeuwen W,van Belkum A,Horrevorts AM,Simons G

更新日期：2008-04-01 00:00:00

abstract：:Human immunodeficiency virus type 1 serotype C was found in 545 of 712 Ethiopian patients by peptide enzyme immunoassay. Serotyping failed in 146 samples due to the absence of V3 antibodies or multiple reactivities. In 6 of 34 samples, discordant results were obtained by serotyping and genotyping, possibly due to dive...

journal_title：Journal of clinical microbiology

authors： Sherefa K,Sällberg M,Johansson B,Salminen M,Sönnerborg A

更新日期：1997-09-01 00:00:00

abstract：:Actinomyces viscosus is a gram-positive, non-acid-fact, facultative, catalase-positive, filamentous, or diphtheroidal microorganism. It was isolated from six canine infections during a period of 1.5 years. The organism was cultured from exudate and flaky granules aspirated from infectious granulomas and empyemas. All ...

journal_title：Journal of clinical microbiology

authors： Davenport AA,Carter GR,Patterson MJ

更新日期：1975-01-01 00:00:00

abstract：:Intravenous and intraarterial catheters were examined microbiologically and morphologically. Bacteria or yeasts were recovered from 38 of the 63 catheters examined, and Staphylococcus epidermidis was present on 29 of the 38 colonized catheters. Examination of unused Teflon catheters ( Jelco ; Surgikos , Inc., Peterbor...

journal_title：Journal of clinical microbiology

authors： Marrie TJ,Costerton JW

更新日期：1984-05-01 00:00:00

abstract：:In a study of 1,486 men attending two sexually transmitted disease clinics, of whom 891 had no symptoms of urethritis, we compared an enzyme immunoassay (EIA) (Baxter-Bartels, formerly Northumbria AntigEnz) of urine sediment to urethral culture for the detection of Chlamydia trachomatis. C. trachomatis prevalence by c...

journal_title：Journal of clinical microbiology

authors： Sanders JW,Hook EW 3rd,Welsh LE,Shepherd ME,Quinn TC

更新日期：1994-01-01 00:00:00

abstract：:Despite its theoretical advantages, direct antimicrobial susceptibility testing (DST) of urine specimens remains controversial largely because of concerns regarding its accuracy, particularly with mixed cultures. To evaluate the performance of DST in the setting of acute urinary tract infection (UTI), we performed DST...

journal_title：Journal of clinical microbiology

authors： Johnson JR,Tiu FS,Stamm WE

更新日期：1995-09-01 00:00:00

abstract：:We have previously reported that the pCS20 PCR detection assay for Cowdria ruminantium, the causative agent of heartwater disease of ruminants, is more sensitive than xenodiagnosis and the pCS20 DNA probe for the detection of infection in the vector Amblyomma ticks. Here, we further assessed the reliability of the PCR...

journal_title：Journal of clinical microbiology

authors： Peter TF,Barbet AF,Alleman AR,Simbi BH,Burridge MJ,Mahan SM

更新日期：2000-04-01 00:00:00

abstract：:It has been difficult to obtain pure Pneumocystis carinii antigen either from cultures or from infected lungs for use in producing a specific antibody against P. carinii. This report describes an approach toward producing a monoclonal antibody that bypasses the antigen purification steps. P. carinii infection was deve...

journal_title：Journal of clinical microbiology

authors： Lee CH,Bolinger CD,Bartlett MS,Kohler RB,Wilde CE 3rd,Smith JW

更新日期：1986-03-01 00:00:00

abstract：:The automated and computerized AutoMicrobic system (AMS; Vitek Systems, Inc., subsidiary of McDonnell Douglas, Hazelwood, Mo.) was evaluated as a means of identifying the Enterobacteriaceae. The Micro-ID system (General Diagnostics, Morris Plains, N.J.) and, when necessary, conventional tubed media were used for compa...

journal_title：Journal of clinical microbiology

authors： Burdash NM,Teti G,West ME,Bannister ER,Manos JP

更新日期：1981-02-01 00:00:00

abstract：:Use of whole-genome sequencing (WGS) for routine mycobacterial species identification and drug susceptibility testing (DST) is becoming a reality. We compared the performances of WGS and standard laboratory workflows prospectively, by parallel processing at a major mycobacterial reference service over the course of 1 ...

journal_title：Journal of clinical microbiology

authors： Quan TP,Bawa Z,Foster D,Walker T,Del Ojo Elias C,Rathod P,MMM Informatics Group.,Iqbal Z,Bradley P,Mowbray J,Walker AS,Crook DW,Wyllie DH,Peto TEA,Smith EG

更新日期：2018-01-24 00:00:00

abstract：:Actinomyces naeslundii is a saprophyte, sometimes a pathogen, of the human oral cavity. Very few extra-oral infections related to this agent have been described. We report the first instance of A. naeslundii as an etiological agent of pelvic actinomycosis in a user of an intrauterine device, an infection so far exclus...

journal_title：Journal of clinical microbiology

authors： Bonnez W,Lattimer G,Mohanraj NA,Johnson TH

更新日期：1985-02-01 00:00:00

abstract：:Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. The available rapid detection methods either lack sensitivity or require complex laboratory manipulation. This study describes a rapid, sensitive detection method that can be easily applied to routine diagnosi...

journal_title：Journal of clinical microbiology

authors： van Elden LJ,Nijhuis M,Schipper P,Schuurman R,van Loon AM

更新日期：2001-01-01 00:00:00

abstract：:Yersinia enterocolitica is known to be associated with gastroenteritis in children and Reiter's syndrome in adults, but it has only rarely been the cause of primary soft tissue infections. A patient with diabetes mellitus developed a calf abscess, from which Y. enterocolitica was isolated in pure culture. Incision, dr...

journal_title：Journal of clinical microbiology

authors： Brennessel DJ,Robbins N,Hindman S

更新日期：1984-08-01 00:00:00

abstract：:We report a case of pneumonia with a fatal outcome caused by a nonhemolytic strain of Streptococcus pyogenes. This strain was isolated in pure growth from blood cultures and was fully identified biochemically. Such strains will be difficult to recognize and isolate from sites with heavy growth of normal flora. This ph...

journal_title：Journal of clinical microbiology

authors： Taylor MB,Barkham T

更新日期：2002-06-01 00:00:00

abstract：:Phialemonium obovatum was found to be the cause of osteomyelitis in a German shepherd dog. Histologic examination of the biopsied material from the left tibia revealed septate, irregularly branched hyphae, swollen cells, and ovate-to-spherical cells divided by a transverse septum. The majority of the fungal elements w...

journal_title：Journal of clinical microbiology

authors： Lomax LG,Cole JR,Padhye AA,Ajello L,Chandler FW,Smith BR

更新日期：1986-05-01 00:00:00

abstract：:To determine that susceptibility of AMPLICOR Chlamydia trachomatis PCR to inhibitory factors possibly present in cervical specimens, we obtained cervical specimens from 200 gynecology patients attending our outpatient clinic. The prevalence of C. trachomatis infection was 4.1%, as determined by cell culture. All AMPLI...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Luijendijk A,Huisman WM,Goessens WH,Kluytmans JA,van Rijsoort-Vos JH,Verbrugh HA

更新日期：1996-12-01 00:00:00

abstract：:Antiretroviral therapy (ART) during acute HIV infection (AHI) interrupts viral dynamics and may delay the emergence of serological markers targeted by current HIV screening and confirmatory assays, thus creating challenges for correctly classifying HIV infection status. The performance of three HIV antigen/antibody co...

journal_title：Journal of clinical microbiology

authors： Manak MM,Jagodzinski LL,Shutt A,Malia JA,Leos M,Ouellette J,Akapirat S,Colby DL,Phanuphak N,Eller LA,Robb ML,de Souza M,Ananworanich J,Peel SA,RV254\/SEARCH010 and the RV217 Study Teams.

更新日期：2019-09-24 00:00:00

abstract：:We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing. ...

journal_title：Journal of clinical microbiology

authors： Ahmed AO,Desplaces N,Leonard P,Goldstein F,De Hoog S,Verbrugh H,van Belkum A

更新日期：2003-12-01 00:00:00

abstract：:Cell line 293, a continuous line of transformed human embryonic kidney cells, has been recognized for its sensitivity in the isolation of adenoviruses, particularly the fastidious species 40 and 41, from stool specimens. To explore the possibility of using this cell line for the isolation of other viruses from clinica...

journal_title：Journal of clinical microbiology

authors： Brown M,Petric M

更新日期：1986-04-01 00:00:00

abstract：:Using the Staphylococcus aureus absorption method, we have modified a commercial radioimmunoassay kit for the determination of total antibody to hepatitis B core antigen so that we can now determine the predominant globulin species of that antibody. One-hundred percent of acute hepatitis B sera had a predominance of i...

journal_title：Journal of clinical microbiology

authors： Schable CA,Maynard JE

更新日期：1982-11-01 00:00:00

abstract：:When Shiga toxin-producing Escherichia coli (STEC) strains emerged as agents of human disease, two types of toxin were identified: Shiga toxin type 1 (Stx1) (almost identical to Shiga toxin produced by Shigella dysenteriae type 1) and the immunologically distinct type 2 (Stx2). Subsequently, numerous STEC strains have...

journal_title：Journal of clinical microbiology

authors： Scheutz F,Teel LD,Beutin L,Piérard D,Buvens G,Karch H,Mellmann A,Caprioli A,Tozzoli R,Morabito S,Strockbine NA,Melton-Celsa AR,Sanchez M,Persson S,O'Brien AD

更新日期：2012-09-01 00:00:00

abstract：:We have conducted an international quality assurance study of filovirus, Lassa virus, and orthopox virus PCR with 24 participants. Of the participating laboratories, 45.8 and 66.7% detected virus in all plasma samples, which contained > or = 5,000 and > or = 100,000 copies per ml, respectively. Sensitivity levels were...

journal_title：Journal of clinical microbiology

authors： Niedrig M,Schmitz H,Becker S,Günther S,ter Meulen J,Meyer H,Ellerbrok H,Nitsche A,Gelderblom HR,Drosten C

更新日期：2004-04-01 00:00:00

abstract：:Several patients receiving blood transfusions during the summer of 1991 developed bacteremia after the transfusion. In all cases, the infection was caused by Serratia marcescens. The same strain of Serratia marcescens was isolated from the patients and from the outer surface of unfilled blood bags. The transport conta...

journal_title：Journal of clinical microbiology

authors： Szewzyk U,Szewzyk R,Stenström TA

更新日期：1993-07-01 00:00:00

abstract：:Fecal specimens from patients with acute diarrhea were collected from 10 prefectures in Japan over a 6-month period (November 1992 to April 1993), and the specimens that were negative for human group A rotaviruses were screened for the presence of human group C rotaviruses (CHRVs) by the reverse passive hemagglutinati...

journal_title：Journal of clinical microbiology

authors： Kuzuya M,Fujii R,Hamano M,Yamada M,Shinozaki K,Sasagawa A,Hasegawa S,Kawamoto H,Matsumoto K,Kawamoto A,Itagaki A,Funatsumaru S,Urasawa S

更新日期：1998-01-01 00:00:00

abstract：:The type strains of six Campylobacter species or subspecies were examined in eight tests used for the identification of such organisms. False-positive results were obtained in certain tests (growth on 1% bile, brilliant green, selenite, trimethylamine-N-oxide, 2,3,5-triphenyl tetrazolium chloride, and minimal media) w...

journal_title：Journal of clinical microbiology

authors： On SL,Holmes B

更新日期：1991-05-01 00:00:00

abstract：:Anthopsis deltoidea was found to be the cause of an olecranon bursitis in a 79-year-old golfer. Serial histological sections of the olecranon bursa showed faintly stained, brown-walled, septate, hyphal elements in the centers of the necrotic debris. The combination of bursectomy and flucytosine treatment cured the inf...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Droller DD

更新日期：1984-08-01 00:00:00