Osteolytic phaeohyphomycosis in a German shepherd dog caused by Phialemonium obovatum.

Abstract:

:Phialemonium obovatum was found to be the cause of osteomyelitis in a German shepherd dog. Histologic examination of the biopsied material from the left tibia revealed septate, irregularly branched hyphae, swollen cells, and ovate-to-spherical cells divided by a transverse septum. The majority of the fungal elements were hyaline, but a few had lightly pigmented cell walls that had a greenish yellow tint. The presence of melanin in the cell walls of the hyphae and especially in their septa was verified by the use of the Fontana-Masson silver stain. P. obovatum formed moist, off-white-to-ochraceous, spreading colonies with a characteristic green pigment on their reverse side. The pigment was more prominent in cultures grown at 37 degrees C than in those grown at 25 degrees C. The isolate also grew at 40 degrees C. The dog isolate produced characteristic adelophialides without conspicuous collarettes and also basal septa from the creeping vegetative hyphae growing on the surface of the medium. The numerous obovate phialoconidia were smooth and one-celled.

journal_name

J Clin Microbiol

authors

Lomax LG,Cole JR,Padhye AA,Ajello L,Chandler FW,Smith BR

doi

10.1128/JCM.23.5.987-991.1986

subject

Has Abstract

pub_date

1986-05-01 00:00:00

pages

987-91

issue

5

eissn

0095-1137

issn

1098-660X

journal_volume

23

pub_type

杂志文章
  • Correlation of Treponemal Immunoassay Signal Strength Values with Reactivity of Confirmatory Treponemal Testing.

    abstract::Automated treponemal immunoassays are used for syphilis screening with the reverse-sequence algorithm; discordant results (e.g., enzyme immunoassay [EIA] reactive and reactive plasma reagin [RPR] nonreactive) are resolved with a second treponemal test. We conducted a study to determine automated immunoassay signal str...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01165-17

    authors: Fakile YF,Jost H,Hoover KW,Gustafson KJ,Novak-Weekley SM,Schapiro JM,Tran A,Chow JM,Park IU

    更新日期:2017-12-26 00:00:00

  • Detection of pneumococci in the upper respiratory tract: comparison of media and culture techniques.

    abstract::A survey for carriers of Streptococcus pneumoniae among 573 children was undertaken. Throat and nasopharyngeal cultures from 303 children disclosed pneumococci in 89 (29.4%) nasopharyngeal swabs but in only 8 (2.6%) throat specimens. Carriers of pneumococci were detected with frequencies of 84.4 and 79.9% on aerobical...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.16.1.1-3.1982

    authors: Robins-Browne RM,Kharsany AB,Ramsaroop UG

    更新日期:1982-07-01 00:00:00

  • Comparison of in-house real-time quantitative PCR to the Adenovirus R-Gene kit for determination of adenovirus load in clinical samples.

    abstract::In the context of hematopoietic stem cell transplantation, adenovirus infections are associated with relevant mortality and morbidity. Detection of adenovirus DNA by quantitative PCR is the "gold standard" for these patients. A total of 150 samples, namely, 78 whole-blood, 22 cerebrospinal fluid, 24 digestive biopsy, ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00976-10

    authors: Jeulin H,Salmon A,Bordigoni P,Venard V

    更新日期:2010-09-01 00:00:00

  • Invasive mycosis due to species of Blastobotrys in immunocompromised patients with reduced susceptibility to antifungals.

    abstract::Cases of invasive mycosis due to Blastobotrys serpentis and B. proliferans identified by sequencing in a preterm patient and a rhabdomyosarcoma patient, respectively, are reported. Both species revealed elevated fluconazole and echinocandin MICs by the CLSI broth microdilution method. Additionally, B. serpentis exhibi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01977-14

    authors: Kumar A,Babu R,Bijulal S,Abraham M,Sasidharan P,Kathuria S,Sharma C,Meis JF,Chowdhary A

    更新日期:2014-11-01 00:00:00

  • Negative correlation of distributions of Streptococcus cristatus and Porphyromonas gingivalis in subgingival plaque.

    abstract::Porphyromonas gingivalis is one of the major causative agents of adult periodontitis. One of the features of this periodontal pathogen is its ability to attach to a variety of oral bacterial surfaces and to colonize subgingival dental plaque. We have shown that Streptococcus cristatus CC5A inhibits expression of fimA,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00072-09

    authors: Wang BY,Wu J,Lamont RJ,Lin X,Xie H

    更新日期:2009-12-01 00:00:00

  • Detection of Mycoplasma pulmonis in cilia-associated respiratory bacillus isolates and in respiratory tracts of rats by nested PCR.

    abstract::To improve the detection of Mycoplasma pulmonis contamination of isolates of cilia-associated respiratory (CAR) bacillus, we developed a nested PCR method using primers for 16S rRNA gene sequences. Of 140 samples of 16 different CAR bacillus isolates, 73 (52%) were inhibitory in the first PCR, as indicated by the abse...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.35.7.1667-1670.1997

    authors: Schoeb TR,Dybvig K,Keisling KF,Davidson MK,Davis JK

    更新日期:1997-07-01 00:00:00

  • Evaluation of an enzyme-linked immunosorbent assay for the detection of ectromelia (mousepox) antibody.

    abstract::Ectromelia virus, an orthopoxvirus that can cause extensive morbidity and mortality (mousepox) in colonized mice, has been epizootically responsible for serious disruption of biomedical research since 1930. The lack of a sensitive and specific serological assay for infection with this virus became apparent during outb...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.18.5.1220-1225.1983

    authors: Buller RM,Bhatt PN,Wallace GD

    更新日期:1983-11-01 00:00:00

  • A Fully Automated Multiplex Assay for Diagnosis of Lyme Disease with High Specificity and Improved Early Sensitivity.

    abstract::Lyme borreliosis is a tick-borne disease caused by the Borrelia burgdorferisensu lato complex. Bio-Rad Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B. burgdorferi The BioPlex 2200 Lyme Total assay exhibits an improved rate of seropositivity in p...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01785-19

    authors: Hahm JB,Breneman JW 4th,Liu J,Rabkina S,Zheng W,Zhou S,Walker RP,Kaul R

    更新日期:2020-04-23 00:00:00

  • Rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by reverse transcription and polymerase chain reaction amplification.

    abstract::A rapid method for detection of respiratory syncytial virus (RSV) in nasopharyngeal aspirates, involving a combination of reverse transcription and polymerase chain reaction amplification (RT-PCR), has been developed. The RT-PCR assay employs oligonucleotide primers specific for the region of the RSV genome which enco...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.4.901-904.1992

    authors: Paton AW,Paton JC,Lawrence AJ,Goldwater PN,Harris RJ

    更新日期:1992-04-01 00:00:00

  • Survival, Persistence, and Isolation of the Emerging Multidrug-Resistant Pathogenic Yeast Candida auris on a Plastic Health Care Surface.

    abstract::The emerging multidrug-resistant pathogenic yeast Candida auris represents a serious threat to global health. Unlike most other Candida species, this organism appears to be commonly transmitted within health care facilities and causes health care-associated outbreaks. To better understand the epidemiology of this emer...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00921-17

    authors: Welsh RM,Bentz ML,Shams A,Houston H,Lyons A,Rose LJ,Litvintseva AP

    更新日期:2017-10-01 00:00:00

  • Mesenteric lymphadenitis of swine caused by Rhodococcus sputi.

    abstract::Rhodococcus sputi caused tuberculosislike lymphadenitis of mesenteric lymph nodes in swine. This is the first study reporting that R. sputi can be a pathogen in swine. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.1.155-157.1988

    authors: Tsukamura M,Komatsuzaki C,Sakai R,Kaneda K,Kudo T,Seino A

    更新日期:1988-01-01 00:00:00

  • Aptima HPV E6/E7 mRNA test is as sensitive as Hybrid Capture 2 Assay but more specific at detecting cervical precancer and cancer.

    abstract::Detection of human papillomavirus (HPV) E6/E7 oncogene expression may be more predictive of cervical cancer risk than testing for HPV DNA. The Aptima HPV test (Gen-Probe) detects E6/E7 mRNA of 14 oncogenic types. Its clinical performance was compared with that of the Hybrid Capture 2 DNA test (HC2; Qiagen) in women re...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02147-10

    authors: Ratnam S,Coutlee F,Fontaine D,Bentley J,Escott N,Ghatage P,Gadag V,Holloway G,Bartellas E,Kum N,Giede C,Lear A

    更新日期:2011-02-01 00:00:00

  • Evaluation of the clinical performance of the cobas 4800 HPV test in patients referred for colposcopy.

    abstract::The clinical performance of the cobas human papillomavirus (HPV) test for detection of high-grade disease in a colposcopy-referred population was compared with that of Hybrid Capture 2 (HC2). The overall agreement between the tests was 92.3%. Clinical sensitivity and specificity for detection of cervical intraepitheli...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01949-13

    authors: White C,Keegan H,Pilkington L,Ruttle C,Kerr P,Sharp L,O'Toole S,Turner M,Prendiville W,D'Arcy T,Fitzpatrick M,Lenehan P,Flannelly G,O'Leary JJ,Martin CM

    更新日期:2013-10-01 00:00:00

  • Comparative typing of Pseudomonas aeruginosa by random amplification of polymorphic DNA or pulsed-field gel electrophoresis of DNA macrorestriction fragments.

    abstract::Eighty-seven strains of Pseudomonas aeruginosa were typed by random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of macrorestriction fragments. Stains were clustered on the basis of interpretative criteria as presented previously for the PFGE analysis. Clusters of strains were al...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.12.3190-3195.1996

    authors: Renders N,Römling U,Verbrugh H,van Belkum A

    更新日期:1996-12-01 00:00:00

  • Use of Haemophilus test medium for broth microdilution antimicrobial susceptibility testing of Streptococcus pneumoniae.

    abstract::A recently described medium (Haemophilus test medium [HTM]) for antimicrobial susceptibility testing of Haemophilus influenzae was evaluated in this study for broth microdilution testing of Streptococcus pneumoniae. A total of 137 clinical isolates was tested against 11 antimicrobial agents, using Mueller-Hinton broth...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.3.430-434.1990

    authors: Jorgensen JH,Maher LA,Howell AW

    更新日期:1990-03-01 00:00:00

  • Isolation and rapid identification of Haemophilus ducreyi.

    abstract::During a 2-month period, 62 strains of Haemophilus ducreyi were isolated from 168 genital lesions and 2 lymph node aspirates. Of these strains, 22 were found on both chocolate agar and fetal bovine serum agar supplemented with vancomycin, 29 were found only on chocolate agar, and 9 were found only on fetal bovine seru...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.16.5.861-864.1982

    authors: Hannah P,Greenwood JR

    更新日期:1982-11-01 00:00:00

  • Multiplex PCR for detection of seven virulence factors and K1/K2 capsular serotypes of Klebsiella pneumoniae.

    abstract::A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is useful for the sur...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02316-14

    authors: Compain F,Babosan A,Brisse S,Genel N,Audo J,Ailloud F,Kassis-Chikhani N,Arlet G,Decré D

    更新日期:2014-12-01 00:00:00

  • Characterization of a Vesivirus Associated with an Outbreak of Acute Hemorrhagic Gastroenteritis in Domestic Dogs.

    abstract::Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence h...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01951-17

    authors: Renshaw RW,Griffing J,Weisman J,Crofton LM,Laverack MA,Poston RP,Duhamel GE,Dubovi EJ

    更新日期:2018-04-25 00:00:00

  • Actinobacillus spp. and related bacteria in infected wounds of humans bitten by horses and sheep.

    abstract::We describe the isolation of Actinobacillus lignieresii and an A. equuli-like bacterium from an infected horse-bite wound in a 22-year-old stable foreman and A. suis from a bite injury in a 35-year-old man who had been attacked by a horse. A. lignieresii was also isolated in pure culture from an infected sheep-bite wo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.11.2535-2538.1991

    authors: Peel MM,Hornidge KA,Luppino M,Stacpoole AM,Weaver RE

    更新日期:1991-11-01 00:00:00

  • IS1311 and IS1245 restriction fragment length polymorphism analyses, serotypes, and drug susceptibilities of Mycobacterium avium complex isolates obtained from a human immunodeficiency virus-negative patient.

    abstract::Six isolates of Mycobacterium avium of genotype dnaJ(+) IS901(-) IS1311(+) IS1245(+) and serotypes 6 (n = 1), 6/9, (n = 2), and 9 (n = 3) were obtained within a 5-month period from a human immunodeficiency virus-negative patient treated for tuberculosis. The isolates were identified with PvuII restriction fragment len...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.10.3712-3719.2002

    authors: Dvorska L,Bartos M,Ostadal O,Kaustova J,Matlova L,Pavlik I

    更新日期:2002-10-01 00:00:00

  • Diagnostic implications of parasite-specific immune responses in immunocompromised patients with strongyloidiasis.

    abstract::Parasite-specific humoral and cellular immune responses were evaluated in in nine immunosuppressed patients with Strongyloides stercoralis infection. Four patients had the disseminated form of the disease, and five had serious manifestations without evidence of hyperinfection. All patients had high levels of immunoglo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.23.6.1099-1103.1986

    authors: Genta RM,Douce RW,Walzer PD

    更新日期:1986-06-01 00:00:00

  • Immunoblot method to detect Streptococcus pneumoniae and identify multiple serotypes from nasopharyngeal secretions.

    abstract::Conventional culture techniques are limited in the ability to detect multiple Streptococcus pneumoniae serotypes in nasopharyngeal (NP) secretions. We developed an immunoblot (IB) method with monoclonal antibodies (MAbs) to detect S. pneumoniae and to identify serotypes. NP specimens stored in skim milk-tryptone-gluco...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.4.1596-1600.2004

    authors: Bronsdon MA,O'Brien KL,Facklam RR,Whitney CG,Schwartz B,Carlone GM

    更新日期:2004-04-01 00:00:00

  • Improved detection of Lassa virus by reverse transcription-PCR targeting the 5' region of S RNA.

    abstract::The method of choice for the detection of Lassa virus is reverse transcription (RT)-PCR. However, the high degree of genetic variability of the virus poses a problem with the design of RT-PCR assays that will reliably detect all strains. Recently, we encountered difficulties in detecting some strains from Liberia and ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02351-09

    authors: Olschläger S,Lelke M,Emmerich P,Panning M,Drosten C,Hass M,Asogun D,Ehichioya D,Omilabu S,Günther S

    更新日期:2010-06-01 00:00:00

  • Dermatitis caused by Neosartorya hiratsukae infection in a hedgehog.

    abstract::This case report describes the fungal dermatitis caused by Neosartorya hiratsukae infection in a household hedgehog confirmed by microscopic examination of conidiophores and DNA analyses including the internal transcribed spacer region, partial beta-tubulin, and the calmodulin gene. It is the first report of a natural...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00737-08

    authors: Han JI,Na KJ

    更新日期:2008-09-01 00:00:00

  • Clinical evaluation of the cartridge-based GeneXpert human papillomavirus assay in women referred for colposcopy.

    abstract::High-risk human papillomavirus (hrHPV) testing is now being introduced as a potential primary screening test for improved detection of cervical precancer and cancer. Current U.S. Food and Drug Administration-approved tests are batch tests that take several hours to complete. A rapid, non-batch test might permit point-...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.00176-14

    authors: Einstein MH,Smith KM,Davis TE,Schmeler KM,Ferris DG,Savage AH,Gray JE,Stoler MH,Wright TC Jr,Ferenczy A,Castle PE

    更新日期:2014-06-01 00:00:00

  • Rapid identification of bacterial pathogens in positive blood culture bottles by use of a broad-based PCR assay coupled with high-resolution melt analysis.

    abstract::We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species le...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00718-10

    authors: Won H,Rothman R,Ramachandran P,Hsieh YH,Kecojevic A,Carroll KC,Aird D,Gaydos C,Yang S

    更新日期:2010-09-01 00:00:00

  • Genetic similarity and phenotypic diversity of commensal and pathogenic strains of Candida albicans isolated from the oral cavity.

    abstract::Colony phenotype and genetic similarity were assessed within and between groups of commensal and pathogenic strains of Candida albicans collected from the oral cavities of individuals in a single geographical locale. Thirty-eight percent of pathogenic isolates contained predominant or minor variant colony morphologies...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.12.3190-3199.1993

    authors: Hellstein J,Vawter-Hugart H,Fotos P,Schmid J,Soll DR

    更新日期:1993-12-01 00:00:00

  • Evaluation of the Osiris expert system for identification of beta-lactam phenotypes in isolates of Pseudomonas aeruginosa.

    abstract::Osiris is a video zone size reader for disk diffusion tests featuring a built-in extended expert system (EES). The efficacy of the EES for the identification of the beta-lactam susceptibility phenotypes of Pseudomonas aeruginosa isolates was evaluated. Thirteen beta-lactams were tested in four laboratories by the disk...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.8.3712-3718.2003

    authors: Bert F,Ould-Hocine Z,Juvin M,Dubois V,Loncle-Provot V,Lefranc V,Quentin C,Lambert N,Arlet G

    更新日期:2003-08-01 00:00:00

  • Detection of Campylobacter jejuni by culture and real-time PCR in a French cohort of patients with Guillain-Barre syndrome.

    abstract::Bacteriological culture and real-time PCR (RT-PCR) were used to detect Campylobacter jejuni in fecal samples from a French cohort of 237 patients with Guillain-Barré syndrome (GBS). We provide evidence that diverse serotypes and genotypes of C. jejuni are a major trigger of GBS in France. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00381-10

    authors: Sivadon-Tardy V,Orlikowski D,Porcher R,Ronco E,Caudie C,Roussi J,Fauchère JL,Mégraud F,Tabor H,Sharshar T,Annane D,Raphaël JC,Gaillard JL

    更新日期:2010-06-01 00:00:00

  • Frequency of microbiologically correct antibiotic therapy increased by infectious disease consultations and microbiological results.

    abstract::In a prospective observational study of bacteremic patients we ascertained the influence of different parts of culture results on the correctness of empirical antibiotic therapy. Ninety-three bacteremic patients requiring antibiotic treatment were included. Patients who had consultations with an infectious disease con...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.06051-11

    authors: Kerremans JJ,Verbrugh HA,Vos MC

    更新日期:2012-06-01 00:00:00