Evaluation of the clinical performance of the cobas 4800 HPV test in patients referred for colposcopy.

Abstract:

:The clinical performance of the cobas human papillomavirus (HPV) test for detection of high-grade disease in a colposcopy-referred population was compared with that of Hybrid Capture 2 (HC2). The overall agreement between the tests was 92.3%. Clinical sensitivity and specificity for detection of cervical intraepithelial neoplasia grade 2 or greater (CIN2+) were 90.0% and 55.5% for cobas and 90.5% and 50.2% for HC2, respectively. In conclusion, both tests showed comparable performance for detection of CIN2+.

journal_name

J Clin Microbiol

authors

White C,Keegan H,Pilkington L,Ruttle C,Kerr P,Sharp L,O'Toole S,Turner M,Prendiville W,D'Arcy T,Fitzpatrick M,Lenehan P,Flannelly G,O'Leary JJ,Martin CM

doi

10.1128/JCM.01949-13

subject

Has Abstract

pub_date

2013-10-01 00:00:00

pages

3415-7

issue

10

eissn

0095-1137

issn

1098-660X

pii

JCM.01949-13

journal_volume

51

pub_type

杂志文章
  • Detection of poliovirus antigen by enzyme immunoassay.

    abstract::A solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Rabbit and guinea pig antisera for the assay were raised against purified poliovirus type 3/Fin (strain 3/Fin/K) isolated from a fecal specimen from a meningitis patient during an outbreak of poliomyelitis in Finland in 1984....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.24.6.954-958.1986

    authors: Ukkonen P,Huovilainen A,Hovi T

    更新日期:1986-12-01 00:00:00

  • Rapid method for identification of six common species of mycobacteria based on multiplex SNP analysis.

    abstract::A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobact...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01084-09

    authors: Wang H,Yue J,Han M,Yang J,Zhao Y

    更新日期:2010-01-01 00:00:00

  • Cryptococcus neoformans var. neoformans (serotype D) strains are more susceptible to heat than C. neoformans var. grubii (serotype A) strains.

    abstract::Cryptococcus neoformans var. neoformans (serotype D) and C. neoformans var. grubii (serotype A) differ in geographic prevalence and dermatotropism, with C. neoformans var. neoformans strains being more prevalent among isolates from temperate countries as well as from skin infections. Analysis of 19 strains from each s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.39.9.3365-3367.2001

    authors: Martinez LR,Garcia-Rivera J,Casadevall A

    更新日期:2001-09-01 00:00:00

  • Preparation and use of erythrocyte-globulin conjugates to Lassa virus in reversed passive hemagglutination and inhibition.

    abstract::Conditions were defined for functional covalent coupling of anti-Lassa virus globulins to glutaraldehyde-fixed chicken erythrocytes. Tolylene-2,4-diisocyanate in a reaction mixture containing not more than 0.01 M NaCl produced uniformly good conjugates which were used in reversed passive hemagglutination (RPH) and rev...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.11.6.593-599.1980

    authors: Goldwasser RA,Elliott LH,Johnson KM

    更新日期:1980-06-01 00:00:00

  • Fluorescent pseudomonads capable of growth at 41 degrees C but distinct from Pseudomonas aeruginosa.

    abstract::One hundred and twenty-seven apyocyanogenic fluorescent Pseudomonas strains capable of growth at 41 degrees C, but differing from Pseudomonas aeruginosa, were typed serologically and tested for pyocin production, antibiotic susceptibility, selected biochemical reactions, and utilization of selected substrates. Results...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Ajello GW,Hoadley AW

    更新日期:1976-11-01 00:00:00

  • Human herpesvirus 8 seroprevalence and evaluation of nonsexual transmission routes by detection of DNA in clinical specimens from human immunodeficiency virus-seronegative patients from central and southern Italy, with and without Kaposi's sarcoma.

    abstract::In order to investigate the seroprevalence of human herpesvirus 8 (HHV-8) infection in central and southern Italy, sera from human immunodeficiency virus (HIV)-seronegative subjects, with and without Kaposi's sarcoma (KS), were analyzed by immunofluorescence assay, using BC-3, a cell line latently infected with HHV-8....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.4.1150-1153.1999

    authors: Cattani P,Capuano M,Cerimele F,La Parola IL,Santangelo R,Masini C,Cerimele D,Fadda G

    更新日期:1999-04-01 00:00:00

  • Clinical correlations of serial quantitative blood cultures determined by lysis-centrifugation in patients with persistent septicemia.

    abstract::The potential clinical value of colony counts determined by the lysis-centrifugation blood culture method was studied by reviewing the records of eight patients with persistent septicemia in whom colony counts were available on at least 3 days. Colony counts of the five patients who survived decreased steadily as the ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.19.6.766-771.1984

    authors: Whimbey E,Wong B,Kiehn TE,Armstrong D

    更新日期:1984-06-01 00:00:00

  • Rapid detection of Panton-Valentine leukocidin in Staphylococcus aureus cultures by use of a lateral flow assay based on monoclonal antibodies.

    abstract::Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spott...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02285-12

    authors: Monecke S,Müller E,Buechler J,Rejman J,Stieber B,Akpaka PE,Bandt D,Burris R,Coombs G,Hidalgo-Arroyo GA,Hughes P,Kearns A,Abós SM,Pichon B,Skakni L,Söderquist B,Ehricht R

    更新日期:2013-02-01 00:00:00

  • Assessment on experimental bacterial biofilms and in clinical practice of the efficacy of sampling solutions for microbiological testing of endoscopes.

    abstract::Opinions differ on the value of microbiological testing of endoscopes, which varies according to the technique used. We compared the efficacy on bacterial biofilms of sampling solutions used for the surveillance of the contamination of endoscope channels. To compare efficacy, we used an experimental model of a 48-h Ps...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.06221-11

    authors: Aumeran C,Thibert E,Chapelle FA,Hennequin C,Lesens O,Traoré O

    更新日期:2012-03-01 00:00:00

  • Comparative cost-effectiveness of two-tiered testing strategies for serodiagnosis of lyme disease with noncutaneous manifestations.

    abstract::The mainstay of laboratory diagnosis for Lyme disease is two-tiered serological testing, in which a reactive first-tier enzyme-linked immunosorbent assay (ELISA) or an immunofluorescence assay is supplemented by separate IgM and IgG immunoblots. Recent data suggest that the C6 ELISA can be substituted for immunoblots ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01853-13

    authors: Wormser GP,Levin A,Soman S,Adenikinju O,Longo MV,Branda JA

    更新日期:2013-12-01 00:00:00

  • Chlamydia trachomatis serovar distribution and Neisseria gonorrhoeae coinfection in male patients with urethritis in Greece.

    abstract::The distribution of Chlamydia trachomatis serovars and Neisseria gonorrhoeae coinfection was studied in a group of 100 C. trachomatis-positive males with urethritis in Greece. The serovar distribution revealed that apart from the predominant worldwide types E and F, the relatively uncommon type G is also prevalent. Go...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00586-10

    authors: Papadogeorgakis H,Pittaras TE,Papaparaskevas J,Pitiriga V,Katsambas A,Tsakris A

    更新日期:2010-06-01 00:00:00

  • Diagnosis of cutaneous leishmaniasis in Colombia: the sampling site within lesions influences the sensitivity of parasitologic diagnosis.

    abstract::Parasitologic confirmation of cutaneous leishmaniasis is obligatory before chemotherapy can be considered. Direct microscopic examination of scrapings taken from indurated borders of ulcers has been routinely used as primary method of diagnosis. In this report we compared the sensitivity of examination of dermal scrap...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.10.3768-3773.2000

    authors: Ramírez JR,Agudelo S,Muskus C,Alzate JF,Berberich C,Barker D,Velez ID

    更新日期:2000-10-01 00:00:00

  • Performance of the COVID19SEROSpeed IgM/IgG Rapid Test, an Immunochromatographic Assay for the Diagnosis of SARS-CoV-2 Infection: a Multicenter European Study.

    abstract::This study assessed the diagnostic performance of the new COVID19SEROSpeed IgM/IgG rapid test (BioSpeedia, a spinoff of the Pasteur Institute of Paris) for the detection of antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in comparison to other commercial antibody assays through a large ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,多中心研究

    doi:10.1128/JCM.02240-20

    authors: Plebani M,Parčina M,Bechri I,Zehender G,Terkeš V,Abdel Hafith B,Antinori S,Pillet S,Gonzalo S,Hoerauf A,Lai A,Morović M,Bourlet T,Torre A,Pozzetto B,Galli M

    更新日期:2021-01-21 00:00:00

  • Choice of reference assay for the detection of rotavirus in fecal specimens: electron microscopy versus enzyme immunoassay.

    abstract::Two previously demonstrated sensitive and specific enzyme immunoassays (EIAs) for rotavirus, one using polyclonal and monoclonal antisera (TestPack Rotavirus [TPK]; Abbott Laboratories) and the other using only monoclonal anti-rotavirus antibodies (Rotaclone [RTC]; Cambridge BioScience Corporation), were evaluated as ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.6.1280-1283.1990

    authors: Dennehy PH,Gauntlett DR,Spangenberger SE

    更新日期:1990-06-01 00:00:00

  • Use of a modified shell vial technique to quantitate cytomegalovirus viremia in a population of solid-organ transplant recipients.

    abstract::A quantitative modification of the shell vial assay was used to investigate cytomegalovirus viremia in solid-organ transplant recipients. The level of viremia detected in 109 of 407 specimens ranged from 0.02 to 28 infectious foci per 100,000 leukocytes. By using a Poisson model, a technique was developed to determine...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.10.2620-2624.1992

    authors: Buller RS,Bailey TC,Ettinger NA,Keener M,Langlois T,Miller JP,Storch GA

    更新日期:1992-10-01 00:00:00

  • Detection and identification of mycobacteria by DNA amplification and oligonucleotide-specific capture plate hybridization.

    abstract::We have developed an easy and rapid detection and identification system for the diagnosis of mycobacterial diseases. The system is based on selective amplification by PCR of mycobacteria with primers based on the genes coding for 16S rRNA. During PCR, a label (digoxigenin-11-dUTP) is incorporated with biotinylated spe...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.11.2994-2998.1995

    authors: De Beenhouwer H,Liang Z,De Rijk P,Van Eekeren C,Portaels F

    更新日期:1995-11-01 00:00:00

  • Molecular characterization of methicillin-resistant Staphylococcus aureus isolates in Korea.

    abstract::We used several molecular typing methods to analyze 196 methicillin-resistant Staphylococcus aureus (MRSA) and 139 methicillin-susceptible S. aureus (MSSA) isolates collected between 1996 and 2005. The sequence type 72 MRSA has increased in frequency in the community in the Republic of Korea and in hospitals in recent...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00098-11

    authors: Kim ES,Lee HJ,Chung GT,Lee YS,Shin DH,Jung SI,Song KH,Park WB,Kim NJ,Park KU,Kim EC,Oh MD,Kim HB

    更新日期:2011-05-01 00:00:00

  • Semiquantitative species-specific detection of Bartonella henselae and Bartonella quintana by PCR-enzyme immunoassay.

    abstract::Bartonella henselae is the main causative agent of cat-scratch disease, and both B. henselae and Bartonella quintana cause angioproliferative disorders such as bacillary angiomatosis. To increase the sensitivity of Bartonella detection by PCR and to improve the species differentiation, we developed a semiquantitative,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.10.3097-3101.1999

    authors: Sander A,Penno S

    更新日期:1999-10-01 00:00:00

  • Identification of Virulent Capnocytophaga canimorsus Isolates by Capsular Typing.

    abstract::Capnocytophaga canimorsus is a dog oral commensal that causes rare but severe infections in humans. C. canimorsus was recently shown to be endowed with a capsular polysaccharide implicated in resistance to the innate immune system of the host. Here, we developed the first C. canimorsus capsular serotyping scheme. We d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00249-17

    authors: Hess E,Renzi F,Koudad D,Dol M,Cornelis GR

    更新日期:2017-06-01 00:00:00

  • Monitoring human immunodeficiency virus type 1-infected patients by ratio of antibodies to gp41 and p24.

    abstract::Antibody responses of 85 patients to human immunodeficiency virus type 1 antigens were quantitated by densitometric analysis of Western blot (immunoblot) assays. All patients had been classified into the following three clinical categories: asymptomatic (ASY), acquired immunodeficiency syndrome (AIDS)-related complex ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.5.843-848.1989

    authors: Schmidt G,Amiraian K,Frey H,Wethers J,Stevens RW,Berns DS

    更新日期:1989-05-01 00:00:00

  • Direct Detection of Emergent Fungal Pathogen Candida auris in Clinical Skin Swabs by SYBR Green-Based Quantitative PCR Assay.

    abstract::The recent emergence of the multidrug-resistant and pathogenic yeast Candida auris continues to cause public health concern worldwide. C. auris is alarming because it causes health care-associated outbreaks and can establish invasive infections with high mortality rates. Transmission between patients is facilitated by...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01337-18

    authors: Sexton DJ,Kordalewska M,Bentz ML,Welsh RM,Perlin DS,Litvintseva AP

    更新日期:2018-11-27 00:00:00

  • Variations in the cytomegalovirus major immediate-early gene found by direct genomic sequencing.

    abstract::An assay to detect and sequence DNA from human cytomegalovirus (HCMV) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction and direct solid-phase sequencing of the amplified material. Urine samples from 16 patients tested positive for HCMV DNA in both a ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.4.955-960.1992

    authors: Brytting M,Wahlberg J,Lundeberg J,Wahren B,Uhlén M,Sundqvist VA

    更新日期:1992-04-01 00:00:00

  • Design and validation of an H5 TaqMan real-time one-step reverse transcription-PCR and confirmatory assays for diagnosis and verification of influenza A virus H5 infections in humans.

    abstract::Increasing diversity among influenza H5N1 viruses has resulted in the need for sensitive and specific diagnostic assays, fully validated for the detection of H5 viruses belonging to all hemagglutinin (HA) clades, particularly the recently circulating H5N1 viruses of clade 2. In this report, the development and validat...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02007-06

    authors: Ellis JS,Smith JW,Braham S,Lock M,Barlow K,Zambon MC

    更新日期:2007-05-01 00:00:00

  • Characterization of toxigenic Corynebacterium ulcerans strains isolated from humans and domestic cats in the United Kingdom.

    abstract::In the United Kingdom there has been a marked increase in the number of human infections caused by toxigenic Corynebacterium ulcerans. During 2002 and 2003 the organism was also isolated from several domestic cats with bilateral nasal discharge. As C. ulcerans has never previously been isolated from cats, the 16S rRNA...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.9.4377-4381.2005

    authors: De Zoysa A,Hawkey PM,Engler K,George R,Mann G,Reilly W,Taylor D,Efstratiou A

    更新日期:2005-09-01 00:00:00

  • Hantavirus disease outbreak in Germany: limitations of routine serological diagnostics and clustering of virus sequences of human and rodent origin.

    abstract::In Europe, hemorrhagic fever with renal syndrome results mainly from infection with Puumala virus (PUUV) or Dobrava virus. For 31 patients from a hantavirus disease outbreak in Lower Bavaria, a district in southeast Germany, serodiagnosis was undertaken by enzyme-linked immunosorbent assay, immunofluorescence assay, a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02573-06

    authors: Schilling S,Emmerich P,Klempa B,Auste B,Schnaith E,Schmitz H,Krüger DH,Günther S,Meisel H

    更新日期:2007-09-01 00:00:00

  • Isolation of Helicobacter pylori from saliva.

    abstract::Helicobacter pylori was grown in low numbers from the saliva of one of nine patients who were positive for gastric H. pylori. The saliva-derived isolate from this patient was identical to the antral biopsy-derived isolate from the same patient and differed from isolates cultured from the antral biopsies of all other p...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.31.10.2802-2804.1993

    authors: Ferguson DA Jr,Li C,Patel NR,Mayberry WR,Chi DS,Thomas E

    更新日期:1993-10-01 00:00:00

  • Six newly recognized types of Streptococcus pneumoniae.

    abstract::The serological properties of six new pneumococcal capsular types are described. A table listing all 90 pneumococcal types and their cross-reactions is included. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.10.2759-2762.1995

    authors: Henrichsen J

    更新日期:1995-10-01 00:00:00

  • Is Chlamydia pneumoniae present in brain lesions of patients with multiple sclerosis?

    abstract::We investigated the presence of Chlamydia pneumoniae in 81 normal and pathological specimens obtained from postmortem brain tissues of patients with multiple sclerosis and with other neurological or nonneurological diseases. The assays used included PCR amplification of all DNA samples in the initial study. Culture an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.11.4274-4276.2000

    authors: Hammerschlag MR,Ke Z,Lu F,Roblin P,Boman J,Kalman B

    更新日期:2000-11-01 00:00:00

  • Detection of antibodies to caprine arthritis-encephalitis virus by protein G enzyme-linked immunosorbent assay and immunoblotting.

    abstract::Sera from goats suffering from caprine arthritis-encephalitis contained antibodies to virus proteins of 15, 17, 28, 40, and 130 kilodaltons in immunoblots of maedi-visna virus. We propose to use immunoblotting as a validation test for enzyme-linked immunosorbent assay and demonstrate that the specificity of indirect e...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.3.580-582.1989

    authors: Zanoni R,Krieg A,Peterhans E

    更新日期:1989-03-01 00:00:00

  • Decreased susceptibility to teicoplanin and vancomycin in coagulase-negative Staphylococci isolated from orthopedic-device-associated infections.

    abstract::We studied 315 coagulase-negative Staphylococcus strains recovered prospectively during 240 surgical procedures (206 subjects) from proven or suspected device-associated bone and joint infections. Sixteen strains (5.1%) had decreased susceptibility to glycopeptides: 15 (12 S. epidermidis strains, 2 S. capitis strains,...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02098-09

    authors: Cremniter J,Slassi A,Quincampoix JC,Sivadon-Tardy V,Bauer T,Porcher R,Lortat-Jacob A,Piriou P,Judet T,Herrmann JL,Gaillard JL,Rottman M

    更新日期:2010-04-01 00:00:00