Abstract:
:We investigated the presence of Chlamydia pneumoniae in 81 normal and pathological specimens obtained from postmortem brain tissues of patients with multiple sclerosis and with other neurological or nonneurological diseases. The assays used included PCR amplification of all DNA samples in the initial study. Culture and a second PCR amplification of the organism in a subset of 19 brain specimens were also performed in two separate laboratories. All results were negative. Thus, this study on a large number of brain tissues suggests that C. pneumoniae is not involved in inflammatory demyelination.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Hammerschlag MR,Ke Z,Lu F,Roblin P,Boman J,Kalman Bdoi
10.1128/JCM.38.11.4274-4276.2000subject
Has Abstractpub_date
2000-11-01 00:00:00pages
4274-6issue
11eissn
0095-1137issn
1098-660Xjournal_volume
38pub_type
杂志文章abstract::We evaluated the clinical performance of Check-Direct CPE for carbapenemase detection directly from 301 perirectal swabs (258 patients) in a nonoutbreak setting. Culture of a PCR-confirmed, carbapenemase-containing organism, or history of colonization with such organism within the previous 2 weeks, was used as the ref...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01921-15
更新日期:2015-12-01 00:00:00
abstract::About 2.5 years after the first isolation of the VanA phenotype of vancomycin-resistant Enterococcus faecium (VREM) in Poland, the first VREM strains with the VanB phenotype have emerged independently in two different Warsaw hospitals. In one of these the VREM strain was selected during the long-term antimicrobial tre...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.2.811-815.2001
更新日期:2001-02-01 00:00:00
abstract::Herpes simplex virus (HSV) infections of the central nervous system (CNS) are associated with significant morbidity and mortality rates in children. This study assessed the impact of a direct HSV (dHSV) PCR assay on the time to result reporting and the duration of acyclovir therapy for children with signs and symptoms...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02559-16
更新日期:2017-05-01 00:00:00
abstract::New Delhi metallo-β-lactamase (NDM)-producing bacteria are considered potential global health threats. It is necessary to monitor NDM-1 and its variants in clinical isolates in order to understand the NDM-1 epidemic and the impact of its variants on β-lactam resistance. To reduce the lengthy time needed for cloning an...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.03363-13
更新日期:2014-06-01 00:00:00
abstract::Two ambient-temperature, dry plasma transport systems, ViveST tubes and RNASound RNA sampling cards, and two extraction methods were compared to frozen plasma for HIV-1 RNA recovery. Significant RNA loss occurred: ViveST+MiniMag > ViveST+QIAamp > RNASound+QIAamp. RNA loss and low specimen volumes may affect the sensit...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00541-16
更新日期:2016-07-01 00:00:00
abstract::With use of the rabbit intestinal loop model, heat-labile enterotoxins from 21 Escherichia coli strains isolated from a wide spectrum of patients with diarrheal diseases were all neutralized to high titer by two antisera prepared against enterotoxins of either E. coli or Vibrio cholerae. These findings suggest marked ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
abstract::Ninety-one isolates of nutritionally variant streptococci (NVS) that were previously isolated from the human mouth were regarded as consisting of 7 Streptococcus defectivus isolates, 78 Streptococcus adjacens isolates, and 6 Gemella morbillorum isolates. However, recent references to the taxonomic reclassification of ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.8.2553-2556.1999
更新日期:1999-08-01 00:00:00
abstract::Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural protein 3AB was used as the antigen in an enzyme-linked immunosorbent assay. This assay allowed the differentiation of vaccinated from infected pigs. Serial studies were performed using sera collected from pigs in the field. Positive reactions wer...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.40.8.2843-2848.2002
更新日期:2002-08-01 00:00:00
abstract::Two closely related species of Rochalimaea, Rochalimaea quintana and Rochalimaea henselae, are nutritionally fastidious but can be cultivated on bacteriologic media from the blood of patients with diverse clinical presentations. We report a case of culture-proven R. henselae bacteremia in a child with persistent fever...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.9.2381-2386.1993
更新日期:1993-09-01 00:00:00
abstract::From November 2000 to October 2001, a reverse transcription-PCR using primers directed to the norovirus RNA polymerase coding region was included in a viral and bacterial routine screening to diagnose sporadic cases of acute gastroenteritis among children in Asturias, Spain. The role of noroviruses (8.6% of the positi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.42.6.2668-2674.2004
更新日期:2004-06-01 00:00:00
abstract::The Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis, (AP), and 20 age- and sex-matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.10.2411-2413.1996
更新日期:1996-10-01 00:00:00
abstract::Two cyp51-related genes (cyp51A and cyp51B) encoding 14-alpha sterol demethylase-like enzymes were identified in the opportunistic human pathogen Aspergillus fumigatus. PCR amplification using degenerate oligonucleotides based on conserved areas of cytochrome P450 demethylases of other filamentous fungi and yeasts all...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.7.2431-2438.2001
更新日期:2001-07-01 00:00:00
abstract::Salmonella enteritidis, serotype Horsham, identified rarely worldwide, was isolated from the stools of six American Indians with gastroenteritis. The patients were from separate tribes located in three geographically distinct villages. Although significant contact was demonstrated between ill individuals within two tr...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.18.1.219-221.1983
更新日期:1983-07-01 00:00:00
abstract::Five strains of an unknown, multidrug-resistant coryneform, gram-positive rod were isolated from blood, bronchial aspirate, and abscess specimens. Four of the five strains isolated were highly resistant to antimicrobial agents, including beta-lactams, aminoglycosides, macrolides, quinolones, and tetracyclines, except ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.8.3713-3717.2005
更新日期:2005-08-01 00:00:00
abstract::Methicillin-resistant coagulase-negative staphylococci were isolated from the nares and skin of 1- to 8-week-old healthy chickens in three flocks from a farm. Isolation of methicillin-resistant coagulase-negative staphylococci was positive for 72 (25.7%) of the 280 chickens tested, with the frequency varying from 2.2 ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.9.2072-2077.1996
更新日期:1996-09-01 00:00:00
abstract::A multilaboratory study designed to determine the in vitro susceptibility criteria and quality control parameters for ofloxacin against Neisseria gonorrhoeae was conducted according to the guidelines of the National Committee for Clinical Laboratory Standards. Proposed susceptibility breakpoints are MICs of less than ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.4.1024-1026.1992
更新日期:1992-04-01 00:00:00
abstract::We surveyed 75 clinical laboratories to determine if National Committee for Clinical Laboratory Standards (NCCLS) were being used for the susceptibility testing of Haemophilus influenzae. Of the 66 laboratories that performed susceptibility testing, all claimed to follow current NCCLS guidelines. However, upon further...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.8.2013-2015.1994
更新日期:1994-08-01 00:00:00
abstract::A fecal PCR assay for detection of Helicobacter infections in laboratory rodents was developed. DNA was isolated from murine fecal pellets, and a region of the 16S rRNA gene conserved among murine Helicobacter species was amplified. The fecal PCR was sensitive and specific. This assay does not require euthanasia of ro...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.6.1620-1623.1997
更新日期:1997-06-01 00:00:00
abstract::During the last 10 to 15 years, Aerococcus-like organisms have been isolated from urinary tract specimens from hospitalized patients at Bispebjerg Hospital, Copenhagen, Denmark, with signs of urinary tract infection, and a single strain has been deposited at the National Collection of Type Cultures (NCTC 12142). An ad...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.5.1049-1053.1991
更新日期:1991-05-01 00:00:00
abstract::The human herpesvirus 6 (HHV-6) early protein, p41, encoded by the U27 gene has been detected in oligodendrocytes of multiple sclerosis (MS) patients by using a monoclonal antibody (MAb to p41/38). We here report the antigenic epitope of HHV-6 p41 recognized by this MAb. First, we established that the MAb to p41/38 re...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.4.1449-1455.2001
更新日期:2001-04-01 00:00:00
abstract::Bacillus cereus is a well-known cause of food-borne illness, but infection with this organism is not commonly reported because of its usually mild symptoms. A fatal case due to liver failure after the consumption of pasta salad is described and demonstrates the possible severity of the emetic syndrome. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.8.4277-4279.2005
更新日期:2005-08-01 00:00:00
abstract::The Triage parasite panel (BIOSITE Diagnostics, San Diego, Calif.) is a new qualitative enzyme immunoassay (EIA) panel for the detection of Giardia lamblia, Entamoeba histolytica/E. dispar, and Cryptosporidium parvum in fresh or fresh, frozen, unfixed human fecal specimens. By using specific antibodies, antigens speci...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.38.9.3337-3340.2000
更新日期:2000-09-01 00:00:00
abstract::Spirochetes of the genus Treponema were cultured from 7 of 10 cases of digital dermatitis in sheep. Two cultures comprised Treponema phagedenis-like and Treponema medium/Treponema vincentii-like spirochetes, respectively, while the remaining cultures comprised mixed populations of Treponema medium/Treponema vincentii-...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01934-08
更新日期:2009-04-01 00:00:00
abstract::The recent emergence of carbapenemase-producing Enterobacteriaceae strains represents a major threat for hospitalized patients. We document the dissemination and control of carbapenemase-producing Klebsiella pneumoniae clones in a Greek hospital. During a 3-year study period (January 2009 to December 2011), carbapenem...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00459-12
更新日期:2012-08-01 00:00:00
abstract::Minimum essential medium supplemented with either 7% fetal bovine serum or 2% fetal bovine serum and 1.5% low protein serum replacement (Sigma Chemical Co., St. Louis, Mo.) factor was used to evaluate the growth of Vero 76 cells (E-6 clone) in a cell culture bioreactor. Three different microcarriers, collagen-coated p...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.2.283-286.1990
更新日期:1990-02-01 00:00:00
abstract::Capnocytophaga canimorsus is a dog oral commensal that causes rare but severe infections in humans. C. canimorsus was recently shown to be endowed with a capsular polysaccharide implicated in resistance to the innate immune system of the host. Here, we developed the first C. canimorsus capsular serotyping scheme. We d...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00249-17
更新日期:2017-06-01 00:00:00
abstract::A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is useful for the sur...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02316-14
更新日期:2014-12-01 00:00:00
abstract::Immunoblots were used to study the immunoglobulin G response to Borrelia burgdorferi in experimentally and naturally exposed dogs. Adsorption studies confirmed that the antibodies were specific for B. burgdorferi. Experimentally exposed dogs were asymptomatic. Naturally exposed dogs included both asymptomatic animals ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.4.648-653.1988
更新日期:1988-04-01 00:00:00
abstract::Leishmania (Viannia) braziliensis causes three main types of American tegumentary leishmaniasis (ATL), localized cutaneous leishmaniasis (CL), mucosal leishmaniasis (ML), and disseminated leishmaniasis (DL). All forms are observed among individuals of Corte de Pedra, Brazil. We previously used random amplified markers...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02064-12
更新日期:2012-12-01 00:00:00
abstract::The recovery of Mycobacterium tuberculosis from sputa positive or negative for acid-fast bacilli that were stored for 17 +/- 7 days and inoculated in the BACTEC MGIT 960 system (MGIT) was higher than that from sputa inoculated in Lowenstein-Jensen medium. MGIT is useful for isolation of M. tuberculosis from sputa subj...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01985-06
更新日期:2007-02-01 00:00:00