Proposed interpretive criteria and quality control parameters for ofloxacin susceptibility testing of Neisseria gonorrhoeae.

abstract：:Of 45 influenza virus strains (43 type A and 2 type B) detected in conventional tube cell cultures (average time, 4 days), 25 (56%) were detected by immunofluorescence in the shell vial assay 24 h postinoculation. The specific fluorescence produced should allow this procedure to be readily adapted by laboratories with...

journal_title：Journal of clinical microbiology

authors： Espy MJ,Smith TF,Harmon MW,Kendal AP

更新日期：1986-10-01 00:00:00

abstract：:Streptococcus pneumoniae is a major cause of bacteremia, meningitis, pneumonia, sinusitis, and acute otitis media in children. Although optochin susceptibility, bile solubility, and Quellung testing are the standards for identifying and differentiating pneumococci, there are several reports of nontypeable pneumococci ...

journal_title：Journal of clinical microbiology

authors： Ing J,Mason EO,Kaplan SL,Lamberth LB,Revell PA,Luna RA,Hulten KG

更新日期：2012-04-01 00:00:00

abstract：:Sequence analysis of an 850-bp fragment internal to the aspecific drug efflux gene ade B revealed 11 sequence types (STs) among a collection of 50 multidrug-resistant Acinetobacter baumannii (MDRAB) strains, including members of pan-European clones I, II, and III. The delineation of STs conformed with the intraspecifi...

journal_title：Journal of clinical microbiology

authors： Huys G,Cnockaert M,Nemec A,Swings J

更新日期：2005-10-01 00:00:00

abstract：:Lipovitellin-salt-mannitol (LSM) plate medium was examined for its ability to directly isolate, recover, and presumptively identify Staphylococcus aureus from 418 clinical specimens. The criteria for medium evaluation included colony morphology reactions, selectivity, and ease of isolation. For 298 specimens used for ...

journal_title：Journal of clinical microbiology

authors： Merlino J,Gill R,Robertson GJ

更新日期：1996-12-01 00:00:00

abstract：:A modified auxanographic carbohydrate assimilation procedure for the identification of medically important yeasts is described. This method employs a heavy inoculum of unstarved yeasts, autoclaved yeast assimilation medium, pour plates of shallow depth, and commercially available carbohydrate-impregnated disks. The ac...

journal_title：Journal of clinical microbiology

authors： Mickelsen PA,McCarthy LR,Propst MA

更新日期：1977-03-01 00:00:00

abstract：:The reported rate of detection of Chlamydia pneumoniae DNA within atherosclerotic lesions by PCR varies between 0 and 100%. In this study, identical sets of coded experimental atheroma samples (n = 15) and spiked controls (n = 5) were analyzed by 16 test methods in nine centers by means of PCR. The positive controls w...

journal_title：Journal of clinical microbiology

authors： Apfalter P,Blasi F,Boman J,Gaydos CA,Kundi M,Maass M,Makristathis A,Meijer A,Nadrchal R,Persson K,Rotter ML,Tong CY,Stanek G,Hirschl AM

更新日期：2001-02-01 00:00:00

abstract：:Four HIV rapid tests were subjected to field validation in Panama and compared to an enzyme-linked immunosorbent assay/Multispot-based testing algorithm. The sensitivities of Determine, Uni-Gold, SD Bioline, and INSTI were 99.8%. The specificities of Determine, SD-Bioline, and Uni-Gold were 100%, and the specificity o...

journal_title：Journal of clinical microbiology

authors： Juarez SI,Nuñez AE,Aranda MM,Mojica D,Kim AA,Parekh B

更新日期：2016-04-01 00:00:00

abstract：:Antibiotic neutralization in blood culture media from two automated systems was evaluated by measuring the recovery of organisms and times to detection in simulated cultures. Overall, BD Bactec Plus media (Bactec FX system) outperformed TREK 80 ml Redox media (VersaTREK system), although results suggest a relative rat...

journal_title：Journal of clinical microbiology

authors： Miller NS,Rogan D,Orr BL,Whitney D

更新日期：2011-04-01 00:00:00

abstract：:A toxic shock syndrome toxin 1 (TSST-1) antibody-binding protein produced by an ovine-associated strain of Staphylococcus aureus was examined. The protein showed total identity to TSST-1 by immunodiffusion analysis. Western blots (immunoblots) of proteins separated by isoelectric focusing revealed that the TSST-1 anti...

journal_title：Journal of clinical microbiology

authors： Ho G,Campbell WH,Carlson E

更新日期：1989-01-01 00:00:00

abstract：:Electrophoretic karyotypes of atypical isolates of Candida albicans, e.g., strains that were germ tube negative, failed to express proteinase activity, demonstrated low virulence for mice, formed hyperchlamydoconidia, produced hyperhyphae, or were sucrose negative (including the type strain of Candida stellatoidea), w...

journal_title：Journal of clinical microbiology

authors： Mahrous M,Lott TJ,Meyer SA,Sawant AD,Ahearn DG

更新日期：1990-05-01 00:00:00

abstract：:As contagious bovine pleuropneumonia (CBPP) is spreading fast in many African countries, there is an increasing demand for rapid and sensitive diagnostic methods that can be used to confirm the initial diagnosis based on clinical symptoms or pathological findings. Two PCR-based diagnostic systems for identification of...

journal_title：Journal of clinical microbiology

authors： Persson A,Pettersson B,Bölske G,Johansson KE

更新日期：1999-12-01 00:00:00

abstract：:Of 35 stool specimens isolated and examined in 293 cells, 15 isolates contained adenovirus species 40 (Ad40), and 4 of these 15 isolates also contained a nonfastidious adenovirus species (Ad1 in two cases, Ad18 or Ad31) which was selected over Ad40 during serial passage in the 293 cells. The selection of Ad1 over Ad40...

journal_title：Journal of clinical microbiology

authors： Brown M

更新日期：1985-08-01 00:00:00

abstract：:Carbohydrate antigen detection, nucleic acid probe detection, and bacterial culture are commonly used to confirm group A streptococcus (GAS) pharyngitis. Compared to standard throat swab specimens, the sensitivities of these tests with mouth specimens are poor. When testing for GAS pharyngitis, the throat remains the ...

journal_title：Journal of clinical microbiology

authors： Fox JW,Marcon MJ,Bonsu BK

更新日期：2006-07-01 00:00:00

abstract：:Acute and convalescent-phase sera obtained from 10 patients infected with a Japanese strain of spotted fever group (SFG) rickettsia were tested by the indirect immunoperoxidase test, the Weil-Felix test, an enzyme-linked immunosorbent assay (ELISA), and immunoblotting. By the Weil-Felix test, the reactivity of these s...

journal_title：Journal of clinical microbiology

authors： Amano K,Hatakeyama H,Okuta M,Suto T,Mahara F

更新日期：1992-09-01 00:00:00

abstract：:Rotavirus is the most common cause of severe dehydrating gastroenteritis in infants. To date, 10 different serotypes of rotavirus have been identified in human stools. While four or five serotypes dominate, serotype circulation varies with season and geography. Since our laboratory has been involved in the development...

journal_title：Journal of clinical microbiology

authors： DiStefano DJ,Kraiouchkine N,Mallette L,Maliga M,Kulnis G,Keller PM,Clark HF,Shaw AR

更新日期：2005-12-01 00:00:00

abstract：:In Korea, vancomycin-resistant enterococci have become important nosocomial pathogens since the late 1990s, and most vancomycin-resistant enterococcal isolates have been VanA phenotype-vanA genotype strains. In 2001, we experienced an outbreak of VanB phenotype-vanA genotype vancomycin-resistant enterococci at a unive...

journal_title：Journal of clinical microbiology

authors： Eom JS,Hwang IS,Hwang BY,Lee JG,Lee YJ,Cheong HJ,Park YH,Park SC,Kim WJ

更新日期：2004-04-01 00:00:00

abstract：:Ectromelia virus, an orthopoxvirus that can cause extensive morbidity and mortality (mousepox) in colonized mice, has been epizootically responsible for serious disruption of biomedical research since 1930. The lack of a sensitive and specific serological assay for infection with this virus became apparent during outb...

journal_title：Journal of clinical microbiology

authors： Buller RM,Bhatt PN,Wallace GD

更新日期：1983-11-01 00:00:00

abstract：:The performance characteristics of three real-time influenza A/B virus reverse transcription-PCR (RT-PCR) assays and two real-time 2009 H1N1 RT-PCR assays were evaluated using previously characterized clinical specimens. A total of 150 respiratory specimens from children (30 influenza A/H1 virus-, 30 influenza A/H3 vi...

journal_title：Journal of clinical microbiology

authors： Selvaraju SB,Selvarangan R

更新日期：2010-11-01 00:00:00

abstract：:We have developed a single-tube multiplex real-time PCR method for the detection of the eight most common Candida species causing septicemia: Candida albicans, C. dubliniensis, C. famata, C. glabrata, C. guilliermondii, C. krusei, C. parapsilosis, and C. tropicalis. The method developed targets the RNase P RNA gene RP...

journal_title：Journal of clinical microbiology

authors： Innings A,Ullberg M,Johansson A,Rubin CJ,Noreus N,Isaksson M,Herrmann B

更新日期：2007-03-01 00:00:00

abstract：:During 1984, the recovery of enteric pathogens from patients with acute diarrhea was enhanced by the use of both rectal swab and stool specimens. With 513 patients for whom both methods were used, the overall recovery rate was increased a minimum of about 10%. Almost 50% of the organisms recovered were detected by onl...

journal_title：Journal of clinical microbiology

authors： Adkins HJ,Santiago LT

更新日期：1987-01-01 00:00:00

abstract：:Candida albicans is an important cause of systemic fungal infections, and rapid diagnostics for identifying and differentiating C. albicans from other Candida species are critical for the timely application of appropriate antimicrobial therapy, improved patient outcomes, and pharmaceutical cost savings. In this work, ...

journal_title：Journal of clinical microbiology

authors： Kim HJ,Brehm-Stecher BF

更新日期：2015-02-01 00:00:00

abstract：:During 1994, a cross-contamination problem leading to false-positive cultures of Mycobacterium tuberculosis was revealed in a mycobacteriology laboratory processing 30,000 to 35,000 samples per year. Molecular strain typing based on restriction fragment length polymorphism confirmed the contaminations. Out of 1,439 po...

journal_title：Journal of clinical microbiology

authors： Bauer J,Thomsen VO,Poulsen S,Andersen AB

更新日期：1997-04-01 00:00:00

abstract：:Group B streptococci (GBS) cause severe invasive disease in both neonates and adults. Understanding the epidemiology of GBS provides information that can include determining disease prevalence rates in defined populations and geographic regions, documenting the success of GBS screening programs, and understanding anti...

journal_title：Journal of clinical microbiology

authors： Alhhazmi A,Hurteau D,Tyrrell GJ

更新日期：2016-07-01 00:00:00

abstract：:The usefulness of Candida ID 2 (CAID2) reformulated medium (bioMérieux, France) has been compared with that of the former Candida ID (CAID; bioMérieux), Albicans ID 2 (ALB2; bioMérieux), and CHROMagar Candida (CAC; Chromagar, France) chromogenic media for the isolation and presumptive identification of clinically rele...

journal_title：Journal of clinical microbiology

authors： Eraso E,Moragues MD,Villar-Vidal M,Sahand IH,González-Gómez N,Pontón J,Quindós G

更新日期：2006-09-01 00:00:00

abstract：:During a survey of Group G and C streptococcal infections of humans two epidemiologically unrelated Group G streptococcal isolates were identified, one from a case of bacteremia and one from a wound infection. These isolates were atypical among this sample in that the emm gene could not be amplified from them by PCR. ...

journal_title：Journal of clinical microbiology

authors： Whatmore AM,Engler KH,Gudmundsdottir G,Efstratiou A

更新日期：2001-11-01 00:00:00

abstract：:Well-defined Legionella pneumophila strains were analyzed by amplification of variable genomic regions with arbitrary and repeat sequence primers. Clinical and environmental outbreak-related isolates showed closely related amplicon patterns. Eleven strains of unrelated origins displayed 10 distinct patterns. Fingerpri...

journal_title：Journal of clinical microbiology

authors： van Belkum A,Struelens M,Quint W

更新日期：1993-08-01 00:00:00

abstract：:This study evaluated the feasibility of a molecular strategy based on identification of Mycobacterium tuberculosis by IS6110 PCR or Cobas Amplicor PCR, and rpoB PCR-DNA sequencing of the 81-bp rifampin resistance determining region (RRDR) for direct detection of rifampin resistance in respiratory specimens. A collecti...

journal_title：Journal of clinical microbiology

authors： Yam WC,Tam CM,Leung CC,Tong HL,Chan KH,Leung ET,Wong KC,Yew WW,Seto WH,Yuen KY,Ho PL

更新日期：2004-10-01 00:00:00

abstract：:Until now, serologic tests that distinguish the closely related human T-cell lymphotropic virus types I (HTLV-I) and II (HTLV-II) infections have not been available. Synthetic peptide assays, employing peptides derived from the core and envelope proteins of HTLV-I and HTLV-II (SynthEIA and Select-HTLV tests), were eva...

journal_title：Journal of clinical microbiology

authors： Lal RB,Heneine W,Rudolph DL,Present WB,Hofhienz D,Hartley TM,Khabbaz RF,Kaplan JE

更新日期：1991-10-01 00:00:00

abstract：:A combination of immunomagnetic separation (IMS) and a polymerase chain reaction (PCR) procedure was used for direct isolation and identification of Shigella dysenteriae type 1 and Shigella flexneri from feces. Immunomagnetic particles were coated with monoclonal antibody MASFB, which is specific for a common epitope ...

journal_title：Journal of clinical microbiology

authors： Islam D,Lindberg AA

更新日期：1992-11-01 00:00:00

abstract：:Ninety-five isolates of Haemophilus paragallinarum were classified serologically by a hemagglutinin serotyping scheme, and the results were compared with results from agglutinin serotyping of the same isolates. Of the isolates, 65 were from Australia, 5 were from Japan, 6 were from the Federal Republic of Germany, 7 w...

journal_title：Journal of clinical microbiology

authors： Eaves LE,Rogers DG,Blackall PJ

更新日期：1989-07-01 00:00:00