Multicenter comparison trial of DNA extraction methods and PCR assays for detection of Chlamydia pneumoniae in endarterectomy specimens.

abstract：:We compared the performance of Organon Teknika's NucliSens and Roche Diagnostic Systems' Monitor quantitative human immunodeficiency type 1 RNA assays. Both had similar linearity and sensitivity over most of the dynamic range of the assays, although the Monitor assay was superior at the low range of RNA values while t...

journal_title：Journal of clinical microbiology

authors： Dyer JR,Pilcher CD,Shepard R,Schock J,Eron JJ,Fiscus SA

更新日期：1999-02-01 00:00:00

abstract：:The sequencing of the VP1 hypervariable region of the human enterovirus (HEV) genome has become the reference test for typing field isolates. This study describes a new strategy for typing HEV at the serotype level that uses a reverse transcription-PCR assay targeting the central part of the VP2 capsid protein. Two pa...

journal_title：Journal of clinical microbiology

authors： Nasri D,Bouslama L,Omar S,Saoudin H,Bourlet T,Aouni M,Pozzetto B,Pillet S

更新日期：2007-08-01 00:00:00

abstract：:Fourth-generation human immunodeficiency virus (HIV) screening immunoassays reduce the diagnostic window between infection and diagnosis by the inclusion of HIV p24 antigen detection together with HIV antibody detection in the same test. We compared third- and fourth-generation HIV immunoassays and a dedicated HIV p24...

journal_title：Journal of clinical microbiology

authors： Speers D,Phillips P,Dyer J

更新日期：2005-10-01 00:00:00

abstract：:Infectious gastroenteritis is one of the most common diseases in young children. To clarify the infectious etiology of diarrhea in Danish children less than 5 years of age, we conducted a 2-year prospective case-control study. Stools from 424 children with diarrhea and 870 asymptomatic age-matched controls were examin...

journal_title：Journal of clinical microbiology

authors： Olesen B,Neimann J,Böttiger B,Ethelberg S,Schiellerup P,Jensen C,Helms M,Scheutz F,Olsen KE,Krogfelt K,Petersen E,Mølbak K,Gerner-Smidt P

更新日期：2005-08-01 00:00:00

abstract：:Molecular analysis of Mycobacterium ulcerans has revealed two new insertion sequences (ISs), IS2404 and IS2606. IS2404 was identified by complete sequencing of a previously described repetitive DNA segment from M. ulcerans. This element is 1,274 bp long, contains 12-bp inverted repeats and a single open reading frame ...

journal_title：Journal of clinical microbiology

authors： Stinear T,Ross BC,Davies JK,Marino L,Robins-Browne RM,Oppedisano F,Sievers A,Johnson PD

更新日期：1999-04-01 00:00:00

abstract：:A method of phage subtyping group A type 49 streptococci is described. The method is similar to that used for phage typing staphylococci, except that lysates obtained by induction with mitomycin C rather than propagated stock phages were used. Five type 49 strains were used as phage donors. Seventy-two strains of type...

journal_title：Journal of clinical microbiology

authors： Skjold SA,Wannamaker LW

更新日期：1976-09-01 00:00:00

abstract：:To investigate whether both tissue culture and PCR on a sequence from the repetitive rDNA could contribute to the diagnosis of toxoplasmosis, blood samples and, if they were available, cerebrospinal fluid (CSF) and aqueous humor samples from 72 human immunodeficiency virus-seropositive patients with suspected toxoplas...

journal_title：Journal of clinical microbiology

authors： Dupon M,Cazenave J,Pellegrin JL,Ragnaud JM,Cheyrou A,Fischer I,Leng B,Lacut JY

更新日期：1995-09-01 00:00:00

abstract：:Ehrlichia canis causes a potentially fatal rickettsial disease of dogs that requires rapid and accurate diagnosis in order to initiate appropriate therapy leading to a favorable prognosis. We recently reported the cloning of two immunoreactive E. canis proteins, P28 and P140, that were applicable for serodiagnosis of ...

journal_title：Journal of clinical microbiology

authors： McBride JW,Corstvet RE,Breitschwerdt EB,Walker DH

更新日期：2001-01-01 00:00:00

abstract：:The nucleotide sequences of the partial rpoB gene were determined from 38 Legionella species, including 15 serogroups of Legionella pneumophila. These sequences were then used to infer the phylogenetic relationships among the Legionella species in order to establish a molecular differentiation method appropriate for t...

journal_title：Journal of clinical microbiology

authors： Ko KS,Lee HK,Park MY,Lee KH,Yun YJ,Woo SY,Miyamoto H,Kook YH

更新日期：2002-07-01 00:00:00

abstract：:Bacterial contamination frequently interferes with successful recovery of the Lyme spirochete from cultures of tissue from Borrelia burgdorferi-infected humans, rodents, or ticks. We used 0.20- and 0.45-microns-pore-size syringe-tip filters to recover spirochetes from cultures contaminated with other bacteria. Low con...

journal_title：Journal of clinical microbiology

authors： Jobe DA,Callister SM,Schell RF

更新日期：1993-07-01 00:00:00

abstract：:We evaluated a new hepatitis C virus RNA assay based on one-stage PCR followed by liquid hybridization with an oligonucleotide probe and compared it with nested-set PCR. The one-stage and nested-set PCR assays had identical sensitivities in analytical experiments and showed 100% concordance when clinical specimens wer...

journal_title：Journal of clinical microbiology

authors： Gretch DR,Wilson JJ,Carithers RL Jr,dela Rosa C,Han JH,Corey L

更新日期：1993-02-01 00:00:00

abstract：:Freshly isolated clinical strains of Klebsiella were tested for the ability to fix dinitrogen by the acetylene reduction assay. Ability to detect this trait was markedly affected by cultural conditions. When the test was run at 37 degrees C in the presence of yeast extract (50 mg/liter), only 1.6% of the organisms wer...

journal_title：Journal of clinical microbiology

authors： Chambers CA,Silver WS

更新日期：1977-11-01 00:00:00

abstract：:Molecular techniques such as spa typing and multilocus sequence typing use DNA sequence data for differentiating Staphylococcus aureus isolates. Although spa typing is capable of detecting both genetic micro- and macrovariation, it has less discriminatory power than the more labor-intensive pulsed-field gel electropho...

journal_title：Journal of clinical microbiology

authors： Koreen L,Ramaswamy SV,Naidich S,Koreen IV,Graff GR,Graviss EA,Kreiswirth BN

更新日期：2005-08-01 00:00:00

abstract：:We previously demonstrated that whole blood contains significantly more hepatitis C virus (HCV) RNA than plasma. To validate the whole-blood-based HCV RNA detection method, a prospective comparison of HCV RNA detection in whole blood and plasma from 50 patients with chronic liver disease was undertaken. Whole-blood an...

journal_title：Journal of clinical microbiology

authors： Stapleton JT,Klinzman D,Schmidt WN,Pfaller MA,Wu P,LaBrecque DR,Han Jq,Phillips MJ,Woolson R,Alden B

更新日期：1999-03-01 00:00:00

abstract：:Early and accurate diagnosis is essential for optimal therapeutic outcomes in patients infected with HIV. Currently, none of the commercially available fourth-generation assays differentiate HIV-1 and HIV-2 antibodies (Ab) or the HIV-1 p24 antigen (Ag). The aim of this study was to evaluate the performance of a novel ...

journal_title：Journal of clinical microbiology

authors： Salmona M,Delarue S,Delaugerre C,Simon F,Maylin S

更新日期：2014-01-01 00:00:00

abstract：:A space-saving media table designed to facilitate pouring large quantities of plated media in a small area is described. ...

journal_title：Journal of clinical microbiology

authors： McGonagle LA,Schmidt G,Eng S,Wirsing K

更新日期：1975-07-01 00:00:00

abstract：:Many central vascular catheters (CVCs) are removed unnecessarily because current diagnostic methods for CVC-associated infection are unreliable. A quantitative PCR assay using primers and probe targeted to bacterial 16S ribosomal DNA was used to measure the levels of bacterial DNA in blood samples drawn through the CV...

journal_title：Journal of clinical microbiology

authors： Warwick S,Wilks M,Hennessy E,Powell-Tuck J,Small M,Sharp J,Millar MR

更新日期：2004-04-01 00:00:00

abstract：:Clostridium difficile is the bacterium most commonly surmised to cause antimicrobial- and hospital-associated diarrhea in developed countries worldwide, and such infections are thought to be increasing in frequency and severity. A laboratory-based study was carried out to characterize C. difficile strains isolated fro...

journal_title：Journal of clinical microbiology

authors： Martin H,Willey B,Low DE,Staempfli HR,McGeer A,Boerlin P,Mulvey M,Weese JS

更新日期：2008-09-01 00:00:00

abstract：:IS6110 restriction fragment length polymorphism (RFLP) genotyping is the most widely used genotyping method to study the epidemiology of Mycobacterium tuberculosis. However, due to the complexity of the IS6110 RFLP genotyping technique, and the interpretation of RFLP data, mycobacterial interspersed repetitive-unit-va...

journal_title：Journal of clinical microbiology

authors： Hanekom M,van der Spuy GD,Gey van Pittius NC,McEvoy CR,Hoek KG,Ndabambi SL,Jordaan AM,Victor TC,van Helden PD,Warren RM

更新日期：2008-10-01 00:00:00

abstract：:An isolate of Proteus mirabilis recovered from blood cultures of a diabetic patient was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole-cell and/or plasmid DNA recovered from the isolate with primers specific for the bla(KPC) carbapenemase ge...

journal_title：Journal of clinical microbiology

authors： Tibbetts R,Frye JG,Marschall J,Warren D,Dunne W

更新日期：2008-09-01 00:00:00

abstract：:The prevalence of the Epstein-Barr virus (EBV) Taiwan variant was investigated in the throat washing (TW) samples from patients with head and neck tumors, persons with nonmalignant diseases, and healthy adults in Taiwan. By using the EBV (BNLF-1 gene)-specific primers and PCR, the EBV latent membrane protein gene BNLF...

journal_title：Journal of clinical microbiology

authors： Jeng KC,Hsu CY,Liu MT,Chung TT,Liu ST

更新日期：1994-01-01 00:00:00

abstract：:Rapid diagnostic testing reduces the turnaround time for pathogen identification in the clinical microbiology laboratory, but the impact on patient care and hospital costs is a matter of speculation. Patel et al. (J. Clin. Microbiol. 55:60-67, 2017, https://doi.org/10.1128/JCM.01452-16) investigate the impact of matri...

journal_title：Journal of clinical microbiology

authors： McElvania TeKippe E

更新日期：2016-12-28 00:00:00

abstract：:Quantitation of hepatitis C virus (HCV) RNA in plasma and serum samples is a costly procedure in both time and reagents. Additionally, cell-associated viral RNA may not be detected. This study evaluated the accuracy of HCV RNA quantitation in small-volume whole-blood (WB) samples, which would be appropriate for point-...

journal_title：Journal of clinical microbiology

authors： Bruns T,Steinmetzer K,Ermantraut E,Stallmach A

更新日期：2009-10-01 00:00:00

abstract：:The ability to rapidly recognize Ebola virus infections is critical to quickly limit further spread of the disease. A rapid, sensitive, and specific laboratory diagnostic test is needed to confirm outbreaks of Ebola virus infection and to distinguish it from other diseases that can cause similar clinical symptoms. A o...

journal_title：Journal of clinical microbiology

authors： Gibb TR,Norwood DA Jr,Woollen N,Henchal EA

更新日期：2001-11-01 00:00:00

abstract：:Sera from goats suffering from caprine arthritis-encephalitis contained antibodies to virus proteins of 15, 17, 28, 40, and 130 kilodaltons in immunoblots of maedi-visna virus. We propose to use immunoblotting as a validation test for enzyme-linked immunosorbent assay and demonstrate that the specificity of indirect e...

journal_title：Journal of clinical microbiology

authors： Zanoni R,Krieg A,Peterhans E

更新日期：1989-03-01 00:00:00

abstract：:The 37-kDa protein (P37) of Borrelia burgdorferi is an antigen that elicits an early immunoglobulin M (IgM) antibody response in Lyme disease patients. The P37 gene was cloned from a B. burgdorferi genomic library by screening with antibody from a Lyme disease patient who had developed a prominent humoral response to ...

journal_title：Journal of clinical microbiology

authors： Gilmore RD Jr,Murphree RL,James AM,Sullivan SA,Johnson BJ

更新日期：1999-03-01 00:00:00

abstract：:High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on ty...

journal_title：Journal of clinical microbiology

authors： Castellanos E,Aranaz A,De Buck J

更新日期：2010-04-01 00:00:00

abstract：:Lyme borreliosis is a tick-borne disease caused by the Borrelia burgdorferisensu lato complex. Bio-Rad Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B. burgdorferi The BioPlex 2200 Lyme Total assay exhibits an improved rate of seropositivity in p...

journal_title：Journal of clinical microbiology

authors： Hahm JB,Breneman JW 4th,Liu J,Rabkina S,Zheng W,Zhou S,Walker RP,Kaul R

更新日期：2020-04-23 00:00:00

abstract：:Anti-idiotypic antibodies (anti-Ids) were raised in a rabbit against a murine monoclonal antibody (MAb) neutralizing the yeast killer toxin produced by a strain of Pichia (Hansenula) anomala. In an immunodiffusion test, the anti-Ids produced in the rabbit recognized the antigen-binding site of the MAb used as the immu...

journal_title：Journal of clinical microbiology

authors： Polonelli L,Morace G

更新日期：1988-03-01 00:00:00

abstract：:Mycobacterium bovis infection in wildlife and feral species is a potential source of infection for livestock and a threat to protected and endangered species. The aim of this study was to identify Spanish wild animal species infected with M. bovis through bacteriological culture and spacer oligonucleotide typing (spol...

journal_title：Journal of clinical microbiology

authors： Aranaz A,De Juan L,Montero N,Sánchez C,Galka M,Delso C,Alvarez J,Romero B,Bezos J,Vela AI,Briones V,Mateos A,Domínguez L

更新日期：2004-06-01 00:00:00