Acetylene reduction (dinitrogen fixation) by clinical isolates of Klebsiella pneumoniae.

abstract：:Gas-liquid chromatographic (GLC) profiles of cellular fatty acids and metabolic products were useful in identifying strains of Peptococcus saccharolyticus, Peptococcus asaccharolyticus, Peptostreptococcus anaerobius, Peptostreptococcus micros, and Streptococcus intermedius. The GLC results supported the recent taxonom...

journal_title：Journal of clinical microbiology

authors： Lambert MA,Armfield AY

更新日期：1979-10-01 00:00:00

abstract：:A latex agglutination test was evaluated as a method for detection and titration of antiplague antibodies. Slide and microtiter techniques using polystyrene latex particles coated with specific fraction-I-antigen of Yersinia pestis were found to be comparable in specificity and sensitivity to serological tests commonl...

journal_title：Journal of clinical microbiology

authors： Suzuki S,Sakakibara H,Hotta S

更新日期：1977-10-01 00:00:00

abstract：:The role of human papillomavirus (HPV) in anogenital carcinogenesis is firmly established, but evidence that supports a similar role in skin remains speculative. Immunosuppressed renal transplant recipients have an increased incidence of viral warts and nonmelanoma skin cancer, and the presence of HPV DNA in these les...

journal_title：Journal of clinical microbiology

authors： Harwood CA,Spink PJ,Surentheran T,Leigh IM,de Villiers EM,McGregor JM,Proby CM,Breuer J

更新日期：1999-11-01 00:00:00

abstract：:The clinical observation has been made that there might be an unacceptable number of false-positive enzyme immunoassay (EIA) test results for IgM among persons suspected of having coccidioidomycosis. Patients with a positive result for IgM by EIA are thought to have a diagnosis of acute coccidioidomycosis. However, th...

journal_title：Journal of clinical microbiology

authors： Kuberski T,Herrig J,Pappagianis D

更新日期：2010-06-01 00:00:00

abstract：:Toxin-specific enzyme immunoassays, cytotoxicity assays, and PCR were used to analyze 48 toxin A-negative, toxin B-positive Clostridium difficile isolates from various geographical sites around the world. All the isolates were negative by the TOX-A TEST and positive by the TOX A/B TEST. A deletion of approximately 1.7...

journal_title：Journal of clinical microbiology

authors： Moncrief JS,Zheng L,Neville LM,Lyerly DM

更新日期：2000-08-01 00:00:00

abstract：:Laboratory tests are essential for confirming sporadic cases and outbreaks of rubella. Detection of rubella virus is often necessary to confirm rubella cases and to identify specimens to be used to characterize wild-type rubella viruses. The sensitivities of four methods for detecting rubella virus infection using thr...

journal_title：Journal of clinical microbiology

authors： Zhu Z,Xu W,Abernathy ES,Chen MH,Zheng Q,Wang T,Zhang Z,Li C,Wang C,He W,Zhou S,Icenogle J

更新日期：2007-09-01 00:00:00

abstract：:Detection of methicillin (meticillin)-resistant Staphylococcus aureus colonization was assessed using combined nose and groin swabs in two commercial PCR assays (the Xpert MRSA assay and the BD GeneOhm MRSA assay). Compared to routine culture, both had similar sensitivities (87.0% versus 84.8%, respectively) and speci...

journal_title：Journal of clinical microbiology

authors： Kelley PG,Grabsch EA,Howden BP,Gao W,Grayson ML

更新日期：2009-11-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek's disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did t...

journal_title：Journal of clinical microbiology

authors： Angamuthu R,Baskaran S,Gopal DR,Devarajan J,Kathaperumal K

更新日期：2012-03-01 00:00:00

abstract：:An examination of 45 clinical isolates of Cryptococcus neoformans revealed an unusually high incidence of C. neoformans var. gattii in South Australia (65%) and in the Northern Territory (95%). In assessing all the available data from Australian isolates of C. neoformans, there appeared to be an endemic focus for the ...

journal_title：Journal of clinical microbiology

authors： Ellis DH

更新日期：1987-02-01 00:00:00

abstract：:The API Staph-Ident system was evaluated as a means for identifying the species of bovine strains of staphylococci routinely isolated from quarter-milk samples. The species identity of 314 of 581 (54%) isolates of staphylococci was correctly determined by this method. The API Staph-Ident system was more accurate in co...

journal_title：Journal of clinical microbiology

authors： Langlois BE,Harmon RJ,Akers K

更新日期：1983-11-01 00:00:00

abstract：:A DNA macroarray containing 465 intragenic amplicons was designed to identify Staphylococcus aureus at the species level and to type S. aureus isolates. The genes selected included those encoding (i) S. aureus-specific proteins, (ii) staphylococcal and enterococcal proteins mediating antibiotic resistance and factors ...

journal_title：Journal of clinical microbiology

authors： Trad S,Allignet J,Frangeul L,Davi M,Vergassola M,Couve E,Morvan A,Kechrid A,Buchrieser C,Glaser P,El-Solh N

更新日期：2004-05-01 00:00:00

abstract：:The replication of human coronavirus strain 229E was observed by using indirect immunofluorescence in infected monolayers of MRC continuous cells. By 8 h after infection, bright cytoplasmic fluorescence was detected in cells infected with human coronavirus 229E. Discrete foci of infection were observed from 8 to 16 h ...

journal_title：Journal of clinical microbiology

authors： Macnaughton MR,Thomas BJ,Davies HA,Patterson S

更新日期：1980-09-01 00:00:00

abstract：:By comparing nucleic acid sequences determined for one of the most variable areas of 23S rRNA genes of 23 Campylobacter strains, we were able to identify regions specific for thermophilic Campylobacter strains. Oligonucleotide primers corresponding to these unique regions were synthesized and used in the polymerase ch...

journal_title：Journal of clinical microbiology

authors： Eyers M,Chapelle S,Van Camp G,Goossens H,De Wachter R

更新日期：1993-12-01 00:00:00

abstract：:Penicillium marneffei is recognized as one of the most frequently detected opportunistic pathogens of AIDS patients in northern Thailand. We undertook a genomic epidemiology study of 64 P. marneffei isolates collected from immunosuppressed patients by pulsed-field gel electrophoresis (PFGE) with restriction enzyme Not...

journal_title：Journal of clinical microbiology

authors： Trewatcharegon S,Sirisinha S,Romsai A,Eampokalap B,Teanpaisan R,Chaiyaroj SC

更新日期：2001-12-01 00:00:00

abstract：:To objectively assess the value of examining multiple sputum specimens in maximizing the sensitivity of detection of Mycobacterium tuberculosis, we retrospectively reviewed the acid-fast bacillus smear and culture results of patients diagnosed with culture-proven pulmonary tuberculosis (TB) at Hennepin County Medical ...

journal_title：Journal of clinical microbiology

authors： Nelson SM,Deike MA,Cartwright CP

更新日期：1998-02-01 00:00:00

abstract：:Developing interpretive breakpoints for any given organism-drug combination requires integration of the MIC distribution, pharmacokinetic and pharmacodynamic parameters, and the relationship between the in vitro activity and outcome from both in vivo and clinical studies. Using data generated by standardized broth mic...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Diekema DJ,Rex JH,Espinel-Ingroff A,Johnson EM,Andes D,Chaturvedi V,Ghannoum MA,Odds FC,Rinaldi MG,Sheehan DJ,Troke P,Walsh TJ,Warnock DW

更新日期：2006-03-01 00:00:00

abstract：:Standard culture methods for diagnosis of Streptococcus pneumoniae pneumonia take at least 24 h. The BinaxNOW urine-based test for S. pneumoniae (BinaxNOW-SP) takes only 15 min to conduct, potentially enabling earlier diagnosis and targeted treatment. This study was conducted to assess whether the use of BinaxNOW-SP a...

journal_title：Journal of clinical microbiology

authors： Sinclair A,Xie X,Teltscher M,Dendukuri N

更新日期：2013-07-01 00:00:00

abstract：:We have developed a sensitive assay for Treponema pallidum subsp. pallidum (T. pallidum), the agent of veneral syphilis, based upon the polymerase chain reaction (PCR). A 658-bp portion of the gene encoding the 47-kDa membrane immunogen was amplified, and the PCR products were probed by DNA-DNA hybridization with a 49...

journal_title：Journal of clinical microbiology

authors： Burstain JM,Grimprel E,Lukehart SA,Norgard MV,Radolf JD

更新日期：1991-01-01 00:00:00

abstract：:The classification of viruses is relevant to a number of scientific and clinical disciplines, including the practice of diagnostic virology. Here, we provide an update to our previous review of taxonomic changes for disease-causing viruses in humans and vertebrate animals, covering changes between 2018 and 2020. Recen...

journal_title：Journal of clinical microbiology

authors： Loeffelholz MJ,Fenwick BW

更新日期：2021-01-21 00:00:00

abstract：:A total of 322 single and paired serum samples from children (newborn to 10 years old), young adults (18 to 20 years old), pregnant women and their cord serum samples, and elderly people (more than 70 years old) was tested for antibodies to enteric adenovirus types 40 and 41 by neutralization test. Serum samples were ...

journal_title：Journal of clinical microbiology

authors： Shinozaki T,Araki K,Ushijima H,Fujii R

更新日期：1987-09-01 00:00:00

abstract：:A VIM metallo-beta-lactamase-producing Aeromonas hydrophila strain carrying an integron-borne bla(VIM-4) gene was isolated from a cirrhotic patient's fecal sample in a Budapest hospital. The variable region of this integron is identical with that of a previously characterized integron from Pseudomonas aeruginosa clini...

journal_title：Journal of clinical microbiology

authors： Libisch B,Giske CG,Kovács B,Tóth TG,Füzi M

更新日期：2008-05-01 00:00:00

abstract：:Sera (920) were tested to evaluate the use of heated versus unheated sera in the hemagglutination treponemal test for syphilis. The heated and unheated samples were tested on the same day with the hemagglutination treponemal test for syphilis kit according to the manufacturer's protocol. Agreement of results between t...

journal_title：Journal of clinical microbiology

authors： Hambie EA,Larsen SA,Perryman MW,Pettit DE,Feeley JC

更新日期：1982-02-01 00:00:00

abstract：:The arbitrary primer polymerase chain reaction (also called random amplified polymorphic DNA, or RAPD) is a DNA fingerprinting method that provides an efficient, sensitive way of discriminating between independent isolates of Histoplasma capsulatum, but its widespread application has been hampered by the arduous 2-day...

journal_title：Journal of clinical microbiology

authors： Woods JP,Kersulyte D,Goldman WE,Berg DE

更新日期：1993-02-01 00:00:00

abstract：:A new principle that uses anti-immunoglobulin M-coated polystyrene microtiter plates for the detection of immunoglobulin M antibodies against Toxoplasma gondii by an enzyme-linked immunosorbent assay was developed. ...

journal_title：Journal of clinical microbiology

authors： Duermeyer W,Wielaard F,van Gruijthuijsen H,Swinkels J

更新日期：1980-12-01 00:00:00

abstract：:We report the isolation of Pseudomonas putrefaciens from an intra-abdominal abscess in a patient with colonic carcinoma and from bile in two patients with biliary tract disease. In all three cases, P. putrefaciens was isolated in mixed culture with enteric bacteria. ...

journal_title：Journal of clinical microbiology

authors： Marne C,Pallarés R,Sitges-Serra A

更新日期：1983-06-01 00:00:00

abstract：:During the 1971 epidemic of Venezuelan equine encephalomyelitis (VEE) in south Texas, 203 suspect VEE cases were evaluated by the Center for Disease Control. Sixty-seven were confirmed as cases of VEE. Laboratory confirmation was accomplished by isolation of VEE virus from a serum specimen taken during the acute illne...

journal_title：Journal of clinical microbiology

authors： Bowen GS,Calisher CH

更新日期：1976-07-01 00:00:00

abstract：:This study compared four methods for serotyping isolates of Haemophilus influenzae. Slide agglutination with commercial antisera (Difco Laboratories and Wellcome Diagnostics), coagglutination (Phadebact Haemophilus Test [Pharmacia Fine Chemicals]), latex agglutination with affinity-purified anticapsular antibody, and ...

journal_title：Journal of clinical microbiology

authors： Himmelreich CA,Barenkamp SJ,Storch GA

更新日期：1985-02-01 00:00:00

abstract：:The PathoTec Rapid I-D System for identifying Enterobacteriaceae was evaluated with 471 cultures. In 4,910 individual test comparisons, 95.5% of the results agreed, with results of only two test strips, those for esculin hydrolysis and urease production, agreeing with conventional tests in less than 94% of the trials....

journal_title：Journal of clinical microbiology

authors： Smith PB,Rhoden DL,Tomfohrde KM

更新日期：1975-04-01 00:00:00

abstract：:We compared 14 molecular assays for their ability to detect the Mycobacterium tuberculosis complex in bronchoalveolar lavage fluid samples. Three approaches were followed. First, by using DNA from Mycobacterium bovis BCG, we determined the detection limits of the assays using routine molecular methods. Second, in orde...

journal_title：Journal of clinical microbiology

authors： Akkerman OW,van der Werf TS,de Boer M,de Beer JL,Rahim Z,Rossen JW,van Soolingen D,Kerstjens HA,van der Zanden AG

更新日期：2013-11-01 00:00:00

abstract：:A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg. ...

journal_title：Journal of clinical microbiology

authors： Zhao Y,Petraitiene R,Walsh TJ,Perlin DS

更新日期：2013-03-01 00:00:00