Fast DNA isolation from Histoplasma capsulatum: methodology for arbitrary primer polymerase chain reaction-based epidemiological and clinical studies.

abstract：:Salmonella enterica subsp. enterica serovar Agona was not frequently encountered in Finland until an increase in rates of isolation among animal and feed was seen in 1994. A small outbreak among cattle farms in the regions of Oulu and Vaasa in northwestern Finland in 1994-1995 included eight farms. After the outbreak,...

journal_title：Journal of clinical microbiology

authors： Lindqvist N,Siitonen A,Pelkonen S

更新日期：2002-10-01 00:00:00

abstract：:Aspergillus fumigatus has widely evolved resistance to the most commonly used class of antifungal chemicals, the azoles. Current methods for identifying azole resistance are time-consuming and depend on specialized laboratories. There is an urgent need for rapid detection of these emerging pathogens at point-of-care t...

journal_title：Journal of clinical microbiology

authors： Yu LS,Rodriguez-Manzano J,Moser N,Moniri A,Malpartida-Cardenas K,Miscourides N,Sewell T,Kochina T,Brackin A,Rhodes J,Holmes AH,Fisher MC,Georgiou P

更新日期：2020-10-21 00:00:00

abstract：:Between January 1988 and December 1989 Aeromonas species were isolated from 45 (1.8%) of 2,480 patients with acute gastroenteritis. No other bacterial enteric pathogens were found in any of these 45 patients. Of the 45 Aeromonas isolates, 35 strains (77.8%) were Aeromonas hydrophila, 7 (15.5%) were Aeromonas sobria, a...

journal_title：Journal of clinical microbiology

authors： Deodhar LP,Saraswathi K,Varudkar A

更新日期：1991-05-01 00:00:00

abstract：:The Centers for Disease Control and Prevention recommended confirming positive screening tests for Chlamydia trachomatis when positive predictive values are <90%. It is accepted that less sensitive tests (i.e., culture and immunoassays) should not be used to confirm the results of more sensitive nucleic acid amplifica...

journal_title：Journal of clinical microbiology

authors： Schachter J,Hook EW,Martin DH,Willis D,Fine P,Fuller D,Jordan J,Janda WM,Chernesky M

更新日期：2005-03-01 00:00:00

abstract：:Campylobacter jejuni isolates from stool samples of patients with domestically acquired sporadic infections and from chicken from retail shops were studied during seasonal peaks from June to September over a 3-year period from 1996 to 1998. A large number of pulsed-field gel electrophoresis (PFGE) genotypes (a combine...

journal_title：Journal of clinical microbiology

authors： Hänninen ML,Perko-Mäkelä P,Pitkälä A,Rautelin H

更新日期：2000-05-01 00:00:00

abstract：:This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on...

journal_title：Journal of clinical microbiology

authors： Hans A,Gaudaire D,Manuguerra JC,Leon A,Gessain A,Laugier C,Berthet N,Zientara S

更新日期：2015-01-01 00:00:00

abstract：:Invasive pulmonary aspergillosis (IPA) is a frequently fatal infection in immunocompromised patients that is difficult to diagnose. Present methods for detection of Aspergillus spp. in bronchoalveolar lavage (BAL) fluid and in tissue vary in sensitivity and specificity. We therefore developed an A. fumigatus-specific ...

journal_title：Journal of clinical microbiology

authors： O'Sullivan CE,Kasai M,Francesconi A,Petraitis V,Petraitiene R,Kelaher AM,Sarafandi AA,Walsh TJ

更新日期：2003-12-01 00:00:00

abstract：:In order to increase the application potential of the direct agglutination test (DAT) for the detection of anti-Leishmania antibodies in human serum samples, we developed an antigen based on stained and freeze-dried Leishmania donovani promastigotes. We describe here the evaluation of the performance of the DAT based ...

journal_title：Journal of clinical microbiology

authors： Meredith SE,Kroon NC,Sondorp E,Seaman J,Goris MG,van Ingen CW,Oosting H,Schoone GJ,Terpstra WJ,Oskam L

更新日期：1995-07-01 00:00:00

abstract：:A new molecular biological approach for the identification of bacteria is described. This approach employs PCR of bacterial cell lysates with conserved primers located in the 16S rRNA sequence flanking a variable region, and analysis of the amplified product was based on the principle of single-strand conformation pol...

journal_title：Journal of clinical microbiology

authors： Widjojoatmodjo MN,Fluit AC,Verhoef J

更新日期：1994-12-01 00:00:00

abstract：:Given that the integration of human papillomavirus type 16 (HPV16) into the host genome occurs preferentially with the disruption of the E2 gene, a ratio of E2 to E7 gene copies is often used as a marker for integration. It is largely undetermined, however, whether ratio estimates are affected by HPV intratypic variat...

journal_title：Journal of clinical microbiology

authors： Jiang M,Baseman JG,Koutsky LA,Feng Q,Mao C,Kiviat NB,Xi LF

更新日期：2009-03-01 00:00:00

abstract：:The analytical performance of the AIX1000 system, a fully automated and recently FDA-cleared rapid plasma reagin (RPR) system, was evaluated by comparison to a manual RPR test in a traditional syphilis testing algorithm. A total of 1,028 consecutive serum samples submitted for syphilis testing to the University of Nor...

journal_title：Journal of clinical microbiology

authors： Sanfilippo AM,Freeman K,Schmitz JL

更新日期：2018-07-26 00:00:00

abstract：:Whole-genome sequencing (WGS) is now routinely performed in clinical microbiology laboratories to assess isolate relatedness. With appropriately developed analytics, the same data can be used for prediction of antimicrobial susceptibility. We assessed WGS data for identification using open-source tools and antibiotic ...

journal_title：Journal of clinical microbiology

authors： Ferreira I,Beisken S,Lueftinger L,Weinmaier T,Klein M,Bacher J,Patel R,von Haeseler A,Posch AE

更新日期：2020-06-24 00:00:00

abstract：:Polyomavirus BK (BKV) establishes latent infection in various human tissues, including the kidney. Reactivation following renal transplantation (RT) may cause BKV-associated nephropathy, leading to graft loss. BKV reactivation is often associated with extensive rearrangements in the BKV noncoding regulatory region (NC...

journal_title：Journal of clinical microbiology

authors： Perets TT,Silberstein I,Rubinov J,Sarid R,Mendelson E,Shulman LM

更新日期：2009-05-01 00:00:00

abstract：:A protocol was optimized for the isolation of Mycobacterium avium subsp. paratuberculosis (MAP) from milk and colostrum, with parameters including chemical decontamination, antibiotics, and different culture media. This study demonstrates that the efficiency of MAP recovery from milk is highly dependent upon the cultu...

journal_title：Journal of clinical microbiology

authors： Bradner L,Robbe-Austerman S,Beitz DC,Stabel JR

更新日期：2013-05-01 00:00:00

abstract：:In a long-term rodent bioassay evaluating the carcinogenicity of triethanolamine, there was equivocal evidence of carcinogenic activity in male B6C3F1 mice, based on a marginal increase in the number of hepatocellular adenomas and hepatoblastomas. Interpretation was complicated by the presence of Helicobacter hepaticu...

journal_title：Journal of clinical microbiology

authors： Fox JG,MacGregor JA,Shen Z,Li X,Lewis R,Dangler CA

更新日期：1998-05-01 00:00:00

abstract：:The utility of combined 16S rRNA (rrs) gene restriction fragment length polymorphism and plasmid profiles to differentiate between and within Campylobacter upsaliensis of human and canine origin was examined. Fourteen distinct rrs gene restriction fragment length polymorphs consisting of bands sized between 1.9 and 4....

journal_title：Journal of clinical microbiology

authors： Stanley J,Jones C,Burnens A,Owen RJ

更新日期：1994-07-01 00:00:00

abstract：:In a large prospective comparison, the illumigene test detected Clostridium difficile in 98% of toxin-positive and 58% of toxin-negative samples confirmed positive by other methods. The Xpert was uniformly sensitive. Most samples with discrepant results had C. difficile concentrations below the illumigene limit of det...

journal_title：Journal of clinical microbiology

authors： Gyorke CE,Wang S,Leslie JL,Cohen SH,Solnick JV,Polage CR

更新日期：2013-01-01 00:00:00

abstract：:During a 2-month period, 62 strains of Haemophilus ducreyi were isolated from 168 genital lesions and 2 lymph node aspirates. Of these strains, 22 were found on both chocolate agar and fetal bovine serum agar supplemented with vancomycin, 29 were found only on chocolate agar, and 9 were found only on fetal bovine seru...

journal_title：Journal of clinical microbiology

authors： Hannah P,Greenwood JR

更新日期：1982-11-01 00:00:00

abstract：:Rapid identification of pathogens from blood cultures can decrease lengths of stay and improve patient outcomes. We evaluated the accuracy of the Verigene Gram-positive blood culture (BC-GP) nucleic acid test for investigational use only (Nanosphere, Inc., Northbrook, IL) for the identification of Gram-positive bacter...

journal_title：Journal of clinical microbiology

authors： Wojewoda CM,Sercia L,Navas M,Tuohy M,Wilson D,Hall GS,Procop GW,Richter SS

更新日期：2013-07-01 00:00:00

abstract：:A PCR specific for spacer regions 33 and 34 of the direct repeat region of the Mycobacterium tuberculosis complex was developed to complement the biochemical differentiation of M. tuberculosis, Mycobacterium bovis, M. bovis BCG, and Mycobacterium africanum subtypes I and II. In addition, this approach was incorporated...

journal_title：Journal of clinical microbiology

authors： Yeboah-Manu D,Yates MD,Wilson SM

更新日期：2001-11-01 00:00:00

abstract：:Since the introduction of antiviral compounds such as lamivudine and famciclovir in the treatment schedules of patients with chronic hepatitis B virus (HBV) infection, the accumulation of a variety of mutations in the HBV polymerase gene has been observed. The selection of these mutations is generally considered the c...

journal_title：Journal of clinical microbiology

authors： Stuyver L,Van Geyt C,De Gendt S,Van Reybroeck G,Zoulim F,Leroux-Roels G,Rossau R

更新日期：2000-02-01 00:00:00

abstract：:Although two phenotypes of the opportunistic pathogen Propionibacterium acnes (types I and II) have been described, epidemiological investigations of their roles in different infections have not been widely reported. Using immunofluorescence microscopy with monoclonal antibodies (MAbs) QUBPa1 and QUBPa2, specific for ...

journal_title：Journal of clinical microbiology

authors： McDowell A,Valanne S,Ramage G,Tunney MM,Glenn JV,McLorinan GC,Bhatia A,Maisonneuve JF,Lodes M,Persing DH,Patrick S

更新日期：2005-01-01 00:00:00

abstract：:Alere i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from children hospitalized...

journal_title：Journal of clinical microbiology

authors： Peters RM,Schnee SV,Tabatabai J,Schnitzler P,Pfeil J

更新日期：2017-04-01 00:00:00

abstract：:A female patient suffered a typical attack of typhoid fever due to a lactose-negative, fully susceptible Salmonella typhi strain. During convalescence she became febrile, and a lactose-fermenting S. typhi strain resistant to ampicillin, chloramphenicol, tetracycline, and trimethoprim was isolated from blood culture. T...

journal_title：Journal of clinical microbiology

authors： Cohen SL,Wylie BA,Sooka A,Koornhof HJ

更新日期：1987-08-01 00:00:00

abstract：:The capsular-like envelope of Legionella pneumophila strains Togus 1 (serotype 2) and Philadelphia 1 (serotype 1) was isolated and purified by column chromatography on Sepharose 6B. Antibody raised in rabbits to these two antigenic materials did not cross-react in gel diffusion. Upon electrophoresis followed by gel di...

journal_title：Journal of clinical microbiology

authors： Smith RA,DiGiorgio S,Darner J,Wilhelm A

更新日期：1981-04-01 00:00:00

abstract：:Increasing frequencies of community-acquired methicillin-resistant Staphylococcus aureus (MRSA) strain isolation have been reported from many countries. The overall prevalence of MRSA in Norway is still very low. MRSA isolates (n = 67) detected between 1995 and 2003 in northern Norway were analyzed by pulsed-field gel...

journal_title：Journal of clinical microbiology

authors： Hanssen AM,Fossum A,Mikalsen J,Halvorsen DS,Bukholm G,Sollid JU

更新日期：2005-05-01 00:00:00

abstract：:A DNA microarray platform, based on the nucleotide sequences of the internal transcribed spacer regions (ITS1 and ITS2) of the rRNA gene, was developed to identify 32 fungal pathogens at the species level. The probe sequences were spotted onto polycarbonate slides with a mini-microarray printer, and after the hybridiz...

journal_title：Journal of clinical microbiology

authors： Sturaro LL,Gonoi T,Busso-Lopes AF,Tararam CA,Levy CE,Lyra L,Trabasso P,Schreiber AZ,Kamei K,Moretti ML

更新日期：2018-04-25 00:00:00

abstract：:AmpC regulatory genes in 21 ceftazidime-resistant clinical isolates of Enterobacter cloacae (MICs of > or = 16 microg/ml) were characterized. All isolates exhibited AmpC overproduction due to AmpD mutation. Additionally, we found two AmpR mutants among the isolates. This is the first report of chromosomal ampR mutatio...

journal_title：Journal of clinical microbiology

authors： Kaneko K,Okamoto R,Nakano R,Kawakami S,Inoue M

更新日期：2005-06-01 00:00:00

abstract：:A convenient assay combining solution hybridization and enzyme immunoassay for DNA-RNA hybrids (polymerase chain reaction-enzyme immunoassay [PCR-EIA]) was developed to detect human immunodeficiency virus type 1 (HIV-1) provirus amplified by the PCR and was compared with oligomer hybridization with 32P-labeled SK19. I...

journal_title：Journal of clinical microbiology

authors： Coutlée F,Saint-Antoine P,Olivier C,Voyer H,Kessous-Elbaz A,Berrada F,Bégin P,Giroux L,Viscidi R

更新日期：1991-11-01 00:00:00

abstract：:We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern Japanese prefecture of Nagasaki. The random amplified polymorphic DNA profiles obtained by using three primers revealed six pat...

journal_title：Journal of clinical microbiology

authors： Yamamoto Y,Kohno S,Koga H,Kakeya H,Tomono K,Kaku M,Yamazaki T,Arisawa M,Hara K

更新日期：1995-12-01 00:00:00