Evaluation of Alere i RSV for Rapid Detection of Respiratory Syncytial Virus in Children Hospitalized with Acute Respiratory Tract Infection.

abstract：:The algorithm for a new identification system was designed on the basis of colony color and morphology on CHROMagar Orientation medium in conjunction with simple biochemical tests such as indole (IND), lysine decarboxylase (LDC), and ornithine decarboxylase (ODC) utilization tests with gram-negative bacilli isolated f...

journal_title：Journal of clinical microbiology

authors： Ohkusu K

更新日期：2000-12-01 00:00:00

abstract：:The Lyme disease-associated spirochete, Borrelia burgdorferi, is maintained in enzootic cycles involving Ixodes ticks and small mammals. Previous studies demonstrated that B. burgdorferi expresses outer surface protein A (OspA) but not OspC when residing in the midgut of unfed ticks. However, after ticks feed on blood...

journal_title：Journal of clinical microbiology

authors： Schwan TG,Piesman J

更新日期：2000-01-01 00:00:00

abstract：:This longitudinal study evaluated the response to periodontal treatment by subjects infected with either JP2 (n = 25) or non-JP2 (n = 25) Aggregatibacter (Actinobacillus) actinomycetemcomitans. Participants were treated during the first 4 months by receiving (i) scaling and root planing, (ii) systemic antibiotic thera...

journal_title：Journal of clinical microbiology

authors： Cortelli SC,Costa FO,Kawai T,Aquino DR,Franco GC,Ohara K,Roman-Torres CV,Cortelli JR

更新日期：2009-07-01 00:00:00

abstract：:The population structure of 3,776 Mycobacterium tuberculosis isolates was determined using variable-number tandem-repeat (VNTR) typing. The degree of clonality was so high that a more relaxed definition of clustering cannot be applied. Among recent immigrants with non-Euro-American isolates, transmission is overestima...

journal_title：Journal of clinical microbiology

authors： Sloot R,Borgdorff MW,de Beer JL,van Ingen J,Supply P,van Soolingen D

更新日期：2013-07-01 00:00:00

abstract：:The importance of euparal as a permanent mounting medium for eggs of parasites and the process of making permanent slides are analyzed and discussed in detail. Studies show that helminth eggs mounted in euparal exhibit excellent optical and drying properties. Euparal also can be used to identify proglottids of Taenia ...

journal_title：Journal of clinical microbiology

authors： Berlin OG,Miller MJ

更新日期：1980-11-01 00:00:00

abstract：:The modification of an auxanographic method used in yeast species identification to determine potassium nitrate (KNO3) assimilation resulted in a simple and inexpensive KNO3 assimilation test medium. The medium provided accurate and reliable results in less than 24 h. ...

journal_title：Journal of clinical microbiology

authors： Pincus DH,Salkin IF,Hurd NJ,Levy IL,Kemna MA

更新日期：1988-02-01 00:00:00

abstract：:Recent reports of natural paratuberculosis (or Johne's disease) in rabbits, foxes, and stoats has focused debate on the presence and importance of wildlife reservoirs in the epidemiology of this disease. This paper describes an extensive study investigating 18 nonruminant wildlife species for evidence of paratuberculo...

journal_title：Journal of clinical microbiology

authors： Beard PM,Daniels MJ,Henderson D,Pirie A,Rudge K,Buxton D,Rhind S,Greig A,Hutchings MR,McKendrick I,Stevenson K,Sharp JM

更新日期：2001-04-01 00:00:00

abstract：:We previously demonstrated that whole blood contains significantly more hepatitis C virus (HCV) RNA than plasma. To validate the whole-blood-based HCV RNA detection method, a prospective comparison of HCV RNA detection in whole blood and plasma from 50 patients with chronic liver disease was undertaken. Whole-blood an...

journal_title：Journal of clinical microbiology

authors： Stapleton JT,Klinzman D,Schmidt WN,Pfaller MA,Wu P,LaBrecque DR,Han Jq,Phillips MJ,Woolson R,Alden B

更新日期：1999-03-01 00:00:00

abstract：:A new disposable plastic tube device and technique for ovum and parasite concentration, with upper and lower chambers separated by a fine screen, was compared with the standard gauze and open-funnel filtration technique as well as with the technique of direct smearing with an acid-fast stain. The new concentrator was ...

journal_title：Journal of clinical microbiology

authors： Zierdt WS

更新日期：1984-11-01 00:00:00

abstract：:Common causes of chronic diarrhea among travelers worldwide include protozoan parasites. The majority of parasitic infections are caused by Giardia duodenalis, Entamoeba histolytica, Cryptosporidium parvum, and Cryptosporidium hominis Similarly, these species cause the majority of parasitic diarrhea acquired in the Un...

journal_title：Journal of clinical microbiology

authors： Madison-Antenucci S,Relich RF,Doyle L,Espina N,Fuller D,Karchmer T,Lainesse A,Mortensen JE,Pancholi P,Veros W,Harrington SM

更新日期：2016-11-01 00:00:00

abstract：:Human immunodeficiency virus type 2 (HIV-2) is naturally resistant to several antiretroviral drugs, including all of the non-nucleoside reverse transcriptase inhibitors and the entry inhibitor T-20, and may have reduced susceptibility to some protease inhibitors. These resistance properties make treatment of HIV-2 pat...

journal_title：Journal of clinical microbiology

authors： Jallow S,Kaye S,Schutten M,Brandin E,Albert J,McConkey SJ,Corrah T,Whittle H,Vanham G,Rowland-Jones S,Janssens W

更新日期：2007-05-01 00:00:00

abstract：:The BACTEC 9240 (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) is a new continuous-monitoring blood culture system that uses internal, fluorescent-CO2 sensors. In a multicenter clinical trial, organism yield and times to detection with the prototype BACTEC 9240 system were compared with those of the BAC...

journal_title：Journal of clinical microbiology

authors： Nolte FS,Williams JM,Jerris RC,Morello JA,Leitch CD,Matushek S,Schwabe LD,Dorigan F,Kocka FE

更新日期：1993-03-01 00:00:00

abstract：:Brevibacterium sp. was isolated from the blood of an acutely ill 18-year-old female with AIDS. The isolate was identified as Brevibacterium casei by use of carbohydrate assimilation tests. Treatment was successful with intravenously administered ciprofloxacin. To our knowledge, this is the first report of sepsis cause...

journal_title：Journal of clinical microbiology

authors： Brazzola P,Zbinden R,Rudin C,Schaad UB,Heininger U

更新日期：2000-09-01 00:00:00

abstract：:Potomac horse fever is an acute systemic equine disease caused by Ehrlichia risticii. Currently, serologic methods are widely used to diagnose this disease. However, serologic methods cannot determine whether the horse is presently infected or has been exposed to ehrlichial antigens in the past. The purpose of the pre...

journal_title：Journal of clinical microbiology

authors： Mott J,Rikihisa Y,Zhang Y,Reed SM,Yu CY

更新日期：1997-09-01 00:00:00

abstract：:The effects of Tween 80 supplementation of liquid culture medium on the formation of the superficial L1 layer of the Mycobacterium avium-Mycobacterium intracellulare complex (MAC) were examined by serological and scanning electron microscopic experiments. Specific antiserum to the glycopeptidolipids on the L1 layer of...

journal_title：Journal of clinical microbiology

authors： Masaki S,Sugimori G,Okamoto A,Imose J,Hayashi Y

更新日期：1991-07-01 00:00:00

abstract：:Standard repetitive extragenic palindromic (REP)-PCR, enterobacterial repetitive intergenic consensus-PCR, and Salmonella enteritidis repetitive element-PCR methods for bacterial strain typing were performed with DNA extracted by boiling members of each of the currently recognized species of human viridans group strep...

journal_title：Journal of clinical microbiology

authors： Alam S,Brailsford SR,Whiley RA,Beighton D

更新日期：1999-09-01 00:00:00

abstract：:The development and application of nucleic acid sequence-based amplification (NASBA) assays for the detection of West Nile (WN) and St. Louis encephalitis (SLE) viruses are reported. Two unique detection formats were developed for the NASBA assays: a postamplification detection step with a virus-specific internal capt...

journal_title：Journal of clinical microbiology

authors： Lanciotti RS,Kerst AJ

更新日期：2001-12-01 00:00:00

abstract：:Among 23 patients carrying methicillin-resistant Staphylococcus aureus (MRSA) in their anterior nares, 6 (26%) also carried methicillin-susceptible S. aureus (MSSA) as less prevalent flora. In 4 of the 6 patients, the MSSA was unrelated to prevalent MRSA, as determined by pulsed-field gel electrophoresis (PFGE), multi...

journal_title：Journal of clinical microbiology

authors： Boundy S,Zhao Q,Fairbanks C,Folgosa L,Climo M,Archer GL

更新日期：2012-02-01 00:00:00

abstract：:A multicenter cross-sectional survey was conducted comparing a commercially available chlamydial optical immunoassay (OIA) to the chlamydial ligase chain reaction (LCR). Endocervical samples from 415 outpatients visiting clinics from three hospitals in South Kalimantan, Indonesia, were evaluated. Relative to the LCR, ...

journal_title：Journal of clinical microbiology

authors： Widjaja S,Cohen S,Brady WE,O'reilly K,Susanto,Wibowo A,Cahyono,Graham RR,Porter KR

更新日期：1999-12-01 00:00:00

abstract：:A total of 525 cerebrospinal fluid (CSF) samples submitted during the 2007 and 2008 enteroviral seasons were included in a study to determine the prevalence of and potential risk factors for invalid Cepheid GeneXpert enterovirus assay (GXEA) results, as well as possible solutions for the problem. The invalid GXEA resu...

journal_title：Journal of clinical microbiology

authors： Sefers SE,Raymer AK,Kilby JT,Persing DH,Tang YW

更新日期：2009-09-01 00:00:00

abstract：:Ewingella americana was isolated from two blood cultures from a 75-year-old male after cholecystectomy. The characteristics of this strain are compared with the reported biochemical characteristics of 44 American strains. Previously described infections and pseudoinfections with E. americana are reviewed. ...

journal_title：Journal of clinical microbiology

authors： Devreese K,Claeys G,Verschraegen G

更新日期：1992-10-01 00:00:00

abstract：:A total of 215 typical strains of Candida albicans were studied for their ability to assimilate 11 carbon compounds. All isolates assimilated lactic acid, ribitol, succinic acid, methyl alpha-D-glucopyranoside. None of the isolates assimilated cellobiose and salicin; 1.9% of the isolates assimilated L-arabinose. Citri...

journal_title：Journal of clinical microbiology

authors： Syverson RE

更新日期：1981-01-01 00:00:00

abstract：:Using the Staphylococcus aureus absorption method, we have modified a commercial radioimmunoassay kit for the determination of total antibody to hepatitis B core antigen so that we can now determine the predominant globulin species of that antibody. One-hundred percent of acute hepatitis B sera had a predominance of i...

journal_title：Journal of clinical microbiology

authors： Schable CA,Maynard JE

更新日期：1982-11-01 00:00:00

abstract：:Fifteen monoclonal antibodies (MAbs) made to coxsackievirus B-3 were tested against a panel of enteroviruses by indirect immunofluorescence. The MAbs included seven which reacted with coxsackievirus B-3 only, five which reacted with more than one enterovirus included in the panel, one which reacted with broad enterovi...

journal_title：Journal of clinical microbiology

authors： Yagi S,Schnurr D,Lin J

更新日期：1992-09-01 00:00:00

abstract：:We have introduced a time-resolved fluorometry (TRF)-based microwell hybridization assay for PCR products in detection of herpes simplex virus (HSV) in cerebrospinal fluid (CSF) specimens. TRF is a sensitive nonradioactive detection technique which involves the use of lanthanide chelates as fluorescent labels. We used...

journal_title：Journal of clinical microbiology

authors： Hukkanen V,Rehn T,Kajander R,Sjöroos M,Waris M

更新日期：2000-09-01 00:00:00

abstract：:Helcococcus kunzii, a nonvirulent member of the human skin flora, has recently been implicated in causing infections in immunosuppressed patients. We report a case of breast abscess associated with H. kunzii in an immunocompetant patient and discuss the criteria used in its identification and our observations of susce...

journal_title：Journal of clinical microbiology

authors： Chagla AH,Borczyk AA,Facklam RR,Lovgren M

更新日期：1998-08-01 00:00:00

abstract：:Thirty-seven bacterial clones producing human recombinant monoclonal antibody Fab fragments (rFabs) reactive to herpes simplex virus (HSV) antigens were selected from a human combinatorial antibody library constructed in a phage-display vector by a panning procedure against an HSV lysate. Thirty-four of the HSV-specif...

journal_title：Journal of clinical microbiology

authors： Cattani P,Rossolini GM,Cresti S,Santangelo R,Burton DR,Williamson RA,Sanna PP,Fadda G

更新日期：1997-06-01 00:00:00

abstract：:A modified CAMP test was used to identify 973 Vibrio cholerae isolates by phenotype. Eltor and non-O1 strains were CAMP positive; classical strains were CAMP negative. Sausage-shaped zones of hemolysis of eltor strains were easily distinguished from narrower bands of non-O1 isolates. For O1 isolates, there was 100% ag...

journal_title：Journal of clinical microbiology

authors： Lesmana M,Subekti D,Tjaniadi P,Pazzaglia G

更新日期：1994-01-01 00:00:00

abstract：:The development of a satisfactory means to reliably distinguish between the two closely related species Candida albicans and Candida dubliniensis in the clinical mycology laboratory has proved difficult because these two species are phenotypically so similar. In this study, we have detected homologues of the pH-regula...

journal_title：Journal of clinical microbiology

authors： Kurzai O,Heinz WJ,Sullivan DJ,Coleman DC,Frosch M,Mühlschlegel FA

更新日期：1999-05-01 00:00:00

abstract：:Direct enzyme-linked immunosorbent assay methods offer several advantages in assessing past or recent exposure to cytomegalovirus (CMV) infection, but there persist many pitfalls in the use of these methods for determining specific immunoglobulin M (IgM). The efficiency of absorption of sera by IgG-coated latex beads,...

journal_title：Journal of clinical microbiology

authors： Joassin L,Reginster M

更新日期：1986-03-01 00:00:00