Time-resolved fluorometry PCR assay for rapid detection of herpes simplex virus in cerebrospinal fluid.

abstract：:Acute and convalescent-phase sera obtained from 10 patients infected with a Japanese strain of spotted fever group (SFG) rickettsia were tested by the indirect immunoperoxidase test, the Weil-Felix test, an enzyme-linked immunosorbent assay (ELISA), and immunoblotting. By the Weil-Felix test, the reactivity of these s...

journal_title：Journal of clinical microbiology

authors： Amano K,Hatakeyama H,Okuta M,Suto T,Mahara F

更新日期：1992-09-01 00:00:00

abstract：:There are several convenient and accurate molecular assays to detect respiratory bacterial infection. The NeoPlex RB-8 detection kit (NeoPlex RB-8) is a new multiplex real-time PCR assay that simultaneously detects Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Haemo...

journal_title：Journal of clinical microbiology

authors： Kim JW,Hong SS,Lee IS,Chi HY,Kim SO,Kim HN,Hong SP

更新日期：2020-01-28 00:00:00

abstract：:A prereduced, anaerobically sterilized system of tubed media (PRAS II; Scott Laboratories, Fiskeville, R.I.) was evaluated for accuracy in the identification of anerobic microorganisms. PRAS II was found to be a rapid and accurate identification system for obligate anaerobes which does not require the use of gas cannu...

journal_title：Journal of clinical microbiology

authors： Beaucage CM,Onderdonk AB

更新日期：1982-09-01 00:00:00

abstract：:Haemophilus influenzae type e biotype 4 was isolated from a single antemortem blood culture obtained from a 60-year-old, white male with abdominal carcinomatosis. ...

journal_title：Journal of clinical microbiology

authors： Cawthern TH,Marraro RV,McCleskey FK

更新日期：1978-01-01 00:00:00

abstract：:Chromosomal DNAs of selected Bacteroides organisms whose relatedness had been previously determined by "conventional" filter-annealing studies (J. L. Johnson, Int. J. Syst. Bacteriol. 28:245, 1978) were further analyzed by restriction endonuclease analysis coupled with the Southern hybridization procedure (E. M. South...

journal_title：Journal of clinical microbiology

authors： Bradbury WC,Murray RG,Mancini C,Morris VL

更新日期：1985-01-01 00:00:00

abstract：:Complement-fixing antibody was detected in 13 of 16 patients with histological and/or culture evidence of infection with Nocardia species. The antigen used was a filtrate of soluble substances secreted into liquid growth medium by cultures of N. asteroides. Apparent false positives reactions were found in three of thr...

journal_title：Journal of clinical microbiology

authors： Shainhouse JZ,Pier AC,Stevens DA

更新日期：1978-11-01 00:00:00

abstract：:An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluoresc...

journal_title：Journal of clinical microbiology

authors： Crepin P,Audry L,Rotivel Y,Gacoin A,Caroff C,Bourhy H

更新日期：1998-04-01 00:00:00

abstract：:Burkholderia pseudomallei causes significant global morbidity and mortality, with the highest disease burden in parts of Asia where culture-based diagnosis is often not available. We prospectively evaluated the Active Melioidosis Detect (AMD; InBios International, USA) lateral flow immunoassay (LFI) for rapid detectio...

journal_title：Journal of clinical microbiology

authors： Woods KL,Boutthasavong L,NicFhogartaigh C,Lee SJ,Davong V,AuCoin DP,Dance DAB

更新日期：2018-06-25 00:00:00

abstract：:Acrophialophora fusispora is an emerging opportunistic fungus capable of causing human infections. The taxonomy of the genus is not yet resolved and, in order to facilitate identification of clinical specimens, we have studied a set of clinical and environmental Acrophialophora isolates by morphological and molecular ...

journal_title：Journal of clinical microbiology

authors： Sandoval-Denis M,Gené J,Sutton DA,Wiederhold NP,Guarro J

更新日期：2015-05-01 00:00:00

abstract：:Whole-house surveillance for healthcare-associated infection is no longer the recommended practice because of the large personnel time investment required. We developed a computer-based tracking system using microbiologic data as an aid in detecting potential outbreaks of healthcare-associated infections on a hospital...

journal_title：Journal of clinical microbiology

authors： Hacek DM,Cordell RL,Noskin GA,Peterson LR

更新日期：2004-03-01 00:00:00

abstract：:Multidrug-resistant tuberculous meningitis is fatal without rapid diagnosis and use of second-line therapy. It is more common in human immunodeficiency virus (HIV)-positive patients. Beijing genotype strains of Mycobacterium tuberculosis are associated with drug resistance, particularly multidrug resistance, and their...

journal_title：Journal of clinical microbiology

authors： Caws M,Thwaites G,Stepniewska K,Nguyen TN,Nguyen TH,Nguyen TP,Mai NT,Phan MD,Tran HL,Tran TH,van Soolingen D,Kremer K,Nguyen VV,Nguyen TC,Farrar J

更新日期：2006-11-01 00:00:00

abstract：:Serotyping Streptococcus pneumoniae is a technique generally confined to reference laboratories, as purchasing pneumococcal antisera is a huge investment. Many attempts have been made to modify serological agglutination techniques to make them more accessible, and more recently developments in serotyping have focused ...

journal_title：Journal of clinical microbiology

authors： Batt SL,Charalambous BM,McHugh TD,Martin S,Gillespie SH

更新日期：2005-06-01 00:00:00

abstract：:We report disseminated adenovirus (ADV) infection in four adult cancer patients presenting with focal pulmonary consolidation. In all cases, ADV was recovered from respiratory specimens and ADV viremia (>1 × 10(5) copies/ml) was determined by a quantitative PCR assay. Despite antiviral therapy, 3 (75%) patients died. ...

journal_title：Journal of clinical microbiology

authors： Lee YJ,Palomino-Guilen P,Babady NE,Lamson DM,St George K,Tang YW,Papanicolaou GA

更新日期：2014-01-01 00:00:00

abstract：:The role of human papillomavirus (HPV) in anogenital carcinogenesis is firmly established, but evidence that supports a similar role in skin remains speculative. Immunosuppressed renal transplant recipients have an increased incidence of viral warts and nonmelanoma skin cancer, and the presence of HPV DNA in these les...

journal_title：Journal of clinical microbiology

authors： Harwood CA,Spink PJ,Surentheran T,Leigh IM,de Villiers EM,McGregor JM,Proby CM,Breuer J

更新日期：1999-11-01 00:00:00

abstract：:A multiresistant strain of Pseudomonas aeruginosa is widespread among cystic fibrosis (CF) patients attending clinics in Liverpool, United Kingdom. Suppression subtractive hybridization was used to identify sequences present in the Liverpool CF epidemic strain but absent from strain PAO1. Using dot blot and PCR amplif...

journal_title：Journal of clinical microbiology

authors： Parsons YN,Panagea S,Smart CH,Walshaw MJ,Hart CA,Winstanley C

更新日期：2002-12-01 00:00:00

abstract：:Infectious gastroenteritis is one of the most common diseases in young children. To clarify the infectious etiology of diarrhea in Danish children less than 5 years of age, we conducted a 2-year prospective case-control study. Stools from 424 children with diarrhea and 870 asymptomatic age-matched controls were examin...

journal_title：Journal of clinical microbiology

authors： Olesen B,Neimann J,Böttiger B,Ethelberg S,Schiellerup P,Jensen C,Helms M,Scheutz F,Olsen KE,Krogfelt K,Petersen E,Mølbak K,Gerner-Smidt P

更新日期：2005-08-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome has been devastating the swine industry since the late 1980s. The disease has been controlled, to some extent, through the use of modified live-attenuated (MLV) vaccines once available. However, such a practice periodically resulted in isolation or detection of vaccine-lik...

journal_title：Journal of clinical microbiology

authors： Key KF,Guenette DK,Yoon KJ,Halbur PG,Toth TE,Meng XJ

更新日期：2003-06-01 00:00:00

abstract：:Sepsis is a major cause of death worldwide among nonhospitalized people and hospitalized patients. A wide range of pathogens are involved, and the correct identification and correct antimicrobial therapy are critical to ensure optimal clinical outcomes. With the recent introduction of matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Foster AG

更新日期：2013-11-01 00:00:00

abstract：:A duplex PCR to detect Bordetella pertussis and Bordetella parapertussis was developed with the insertion sequences IS481 (B. pertussis) and IS1001 (B. parapertussis) and evaluated with specimens from 520 consecutive patients presenting with possible pertussis. No culture-positive-PCR-negative results occurred, giving...

journal_title：Journal of clinical microbiology

authors： Farrell DJ,Daggard G,Mukkur TK

更新日期：1999-03-01 00:00:00

abstract：:We developed a loop-mediated isothermal amplification method able to detect Yersinia pseudotuberculosis strains in 30 min by using six primers designed by targeting the inv gene. This method is more sensitive than PCR and might be a useful tool for detecting and identifying Y. pseudotuberculosis. ...

journal_title：Journal of clinical microbiology

authors： Horisaka T,Fujita K,Iwata T,Nakadai A,Okatani AT,Horikita T,Taniguchi T,Honda E,Yokomizo Y,Hayashidani H

更新日期：2004-11-01 00:00:00

abstract：:A total of 563 specimens (234 dermal and 329 genital swabs) from patients suspected of having herpes simplex virus (HSV) infections were processed using two different extraction methods (the MagNA Pure LC system and the swab extraction tube system [SETS]); HSV DNA was amplified by LightCycler PCR. HSV DNA was detected...

journal_title：Journal of clinical microbiology

authors： Issa NC,Espy MJ,Uhl JR,Harmsen WS,Mandrekar JN,Gullerud RE,Davis MD,Smith TF

更新日期：2005-03-01 00:00:00

abstract：:Blood specimens collected from adult patients with suspected sepsis in four medical centers were inoculated into BACTEC Plus/F and BacT/Alert FAN aerobic culture bottles. Both bottles of 7,401 bottle pairs contained the prescribed blood volume of 8 to 12 ml. Bottles were incubated in their respective instruments for a...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Mirrett S,McDonald LC,Murray PR,Weinstein MP,Fune J,Trippy CW,Masterson M,Reller LB

更新日期：1997-01-01 00:00:00

abstract：:A simple new agar medium containing L-canavanine, glycine, and bromthymol blue was found to give a clearer and more accurate distinction between serotype A or D (Cryptococcus neoformans var. neoformans) and serotype B or C (C. neoformans var. gattii) than creatinine-dextrose-bromthymol blue or glycine-cycloheximide-ph...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Polacheck I,Bennett JE

更新日期：1982-03-01 00:00:00

abstract：:Clinical isolates of Haemophilus influenzae were assessed for the presence of fucK, hap, and sodC by hybridization with gene-specific probes, and isolates diverging from the expected H. influenzae genotype were characterized by phenotype and 16S rRNA gene sequencing. Two of 480 isolates were finally classified as vari...

journal_title：Journal of clinical microbiology

authors： Nørskov-Lauritsen N

更新日期：2009-08-01 00:00:00

abstract：:A group of 76 consecutive human immunodeficiency virus (HIV)-positive patients with fever of unknown origin (n = 52) or fever associated with pulmonary diseases was evaluated in order to assess the usefulness of PCR with peripheral blood in the diagnosis and follow-up of visceral leishmaniasis. We identified 10 cases ...

journal_title：Journal of clinical microbiology

authors： Pizzuto M,Piazza M,Senese D,Scalamogna C,Calattini S,Corsico L,Persico T,Adriani B,Magni C,Guaraldi G,Gaiera G,Ludovisi A,Gramiccia M,Galli M,Moroni M,Corbellino M,Antinori S

更新日期：2001-01-01 00:00:00

abstract：:Detection of herpes simplex virus in clinical specimens by the suspension-infection technique was compared with detection by conventional cell culture. The sensitivity and specificity of the suspension-infection technique compared with those of conventional culture were 95.9 to 98.2% and 97.5 to 100%, respectively, de...

journal_title：Journal of clinical microbiology

authors： Luker G,Chow C,Richards DF,Johnson FB

更新日期：1991-07-01 00:00:00

abstract：:A new commercial method intended to exclude the presence of Salmonella spp., Shigella spp., and Yersinia enterocolitica and to presumptively identify Salmonella isolates within 2 h after primary isolation from stool specimens was evaluated. This system is marketed in Europe as API Z and in the United States as Rapid S...

journal_title：Journal of clinical microbiology

authors： Teti G,Burdash NM,Zamboni C,Fava C,Tomasello F,Mastroeni P

更新日期：1984-12-01 00:00:00

abstract：:We recently developed a molecular typing system for Campylobacter jejuni and Campylobacter coli based on restriction fragment length polymorphism analysis of the flagellin gene,flaA (I.Nachamkin, K. Bohachick, and C.M. Patton, J. Clin. Microbiol. 31:1531-1536, 1993). We extended the typing system to 83 flagellin types...

journal_title：Journal of clinical microbiology

authors： Nachamkin I,Ung H,Patton CM

更新日期：1996-02-01 00:00:00

abstract：:We describe here the use of an immunomagnetic separation enrichment process coupled with a modified real-time cellular analysis (RTCA) system (RTCA version 2) for the detection of C. difficile toxin (CDT) in stool. The limit of CDT detection by RTCA version 2 was 0.12 ng/ml. Among the consecutively collected 401 diarr...

journal_title：Journal of clinical microbiology

authors： Huang B,Jin D,Zhang J,Sun JY,Wang X,Stiles J,Xu X,Kamboj M,Babady NE,Tang YW

更新日期：2014-04-01 00:00:00

abstract：:The standardized disk diffusion test, in which a 10-micrograms enoxacin disk is used, was performed and microbroth dilution MICs were determined to establish individual test control values with Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, and S. aureus ATCC 29213. I...

journal_title：Journal of clinical microbiology

authors： Rudrik JT,Cavalieri SJ,Britt EM

更新日期：1985-03-01 00:00:00