Evaluation of a rapid method to exclude the presence of certain enteric pathogens in stool specimens.

Abstract:

:A new commercial method intended to exclude the presence of Salmonella spp., Shigella spp., and Yersinia enterocolitica and to presumptively identify Salmonella isolates within 2 h after primary isolation from stool specimens was evaluated. This system is marketed in Europe as API Z and in the United States as Rapid SST. The strip consists of five pairs of cupules for the screening of five lactose-negative colonies. The first cupule of each pair detects the presence of five enzymatic activities, whereas the second serves to maintain the strain for additional testing if necessary. A total of 197 fresh isolates from stool specimens and 217 stock cultures of Salmonella spp., Shigella spp., and Yersinia enterocolitica were tested, with the API 20E system as a reference method. In the stool specimens, 77.3% of the bacteria could be excluded from further workup for the presence of these organisms within 2 h. Over 97% of the stock strains and each of three fresh Salmonella isolates tested produced a reaction pattern corresponding to a correct presumptive identification. This reaction pattern was not produced by any isolate other than the Salmonella isolates. The API Z system can be used as a screen for the presence of Salmonella and Shigella spp. and can provide an accurate presumptive identification of Salmonella isolates within 2 h after primary isolation.

journal_name

J Clin Microbiol

authors

Teti G,Burdash NM,Zamboni C,Fava C,Tomasello F,Mastroeni P

doi

10.1128/JCM.20.6.1049-1052.1984

subject

Has Abstract

pub_date

1984-12-01 00:00:00

pages

1049-52

issue

6

eissn

0095-1137

issn

1098-660X

journal_volume

20

pub_type

杂志文章
  • Evaluation of the automicrobic system for susceptibility testing of Pseudomonas aeruginosa to gentamicin, tobramycin, and amikacin.

    abstract::The AutoMicrobic system (AMS; Vitek Systems, Inc., Hazelwood, Mo.) was studied for its ability to produce accurate and precise MIC interpretations for Pseudomonas aeruginosa susceptibility to gentamicin, tobramycin, and amikacin. MICs were determined in parallel on 200 selected P. aeruginosa isolates by using the AMS ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.19.4.502-505.1984

    authors: Woolfrey BF,Lally RT,Ederer MN,Quall CO

    更新日期:1984-04-01 00:00:00

  • Sodium polyanethole sulfonate as an inhibitor of activation of complement function in blood culture systems.

    abstract::Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors. We analyzed the effect of SPS on the three complement activation pathwa...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01985-09

    authors: Palarasah Y,Skjoedt MO,Vitved L,Andersen TE,Skjoedt K,Koch C

    更新日期:2010-03-01 00:00:00

  • In vitro evaluation of pyridine-2-azo-p-dimethylaniline cephalosporin, a new diagnostic chromogenic reagent, and comparison with nitrocefin, cephacetrile, and other beta-lactam compounds.

    abstract::Pyridine-2-azo-p-dimethylanaline cephalosporin (PADAC), a chromogenic reagent which is purple and changes to yellow upon cleavage of its beta-lactam ring, was evaluated in comparison with other chromogenic cephalosporins. PADAC exhibited little antimicrobial activity against gram-negative bacteria, but did have good a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.15.4.677-683.1982

    authors: Jones RN,Wilson HW,Novick WJ Jr

    更新日期:1982-04-01 00:00:00

  • Comparison of the Raji cell line fluorescent antibody to membrane antigen test and the enzyme-linked immunosorbent assay for determination of immunity to varicella-zoster virus.

    abstract::A prospective study was performed comparing the fluorescent antibody to membrane antigen (FAMA) test and the enzyme-linked immunosorbent assay (ELISA) for identifying susceptibility and seroconversion to varicella-zoster virus (VZV) infection. A total of 75 sera were collected from index cases and from sibling and par...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.16.5.878-884.1982

    authors: Iltis JP,Castellano GA,Gerber P,Le C,Vujcic LK,Quinnan GV Jr

    更新日期:1982-11-01 00:00:00

  • Evaluation of Unbiased Next-Generation Sequencing of RNA (RNA-seq) as a Diagnostic Method in Influenza Virus-Positive Respiratory Samples.

    abstract::Unbiased nontargeted metagenomic RNA sequencing (UMERS) has the advantage to detect known as well as unknown pathogens and, thus, can significantly improve the detection of viral, bacterial, parasitic, and fungal sequences in public health settings. In particular, conventional diagnostic methods successfully identify ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02495-14

    authors: Fischer N,Indenbirken D,Meyer T,Lütgehetmann M,Lellek H,Spohn M,Aepfelbacher M,Alawi M,Grundhoff A

    更新日期:2015-07-01 00:00:00

  • Evaluation of novel broad-range real-time PCR assay for rapid detection of human pathogenic fungi in various clinical specimens.

    abstract::In the present study, a novel broad-range real-time PCR was developed for the rapid detection of human pathogenic fungi. The assay targets a part of the 28S large-subunit ribosomal RNA (rDNA) gene. We investigated its application for the most important human pathogenic fungal genera, including Aspergillus, Candida, Cr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02178-07

    authors: Vollmer T,Störmer M,Kleesiek K,Dreier J

    更新日期:2008-06-01 00:00:00

  • Reemergence of emm1 and a changed superantigen profile for group A streptococci causing invasive infections: results from a nationwide study.

    abstract::Between 1999 and 2002, 496 invasive group A streptococcal (GAS) isolates from clinical microbiological departments in Denmark and subsequently 487 (98%) questionnaires from the clinicians treating the patients were received as part of a national surveillance. emm types and streptococcal superantigen (SAg) genes were d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.4.1789-1796.2005

    authors: Ekelund K,Skinhøj P,Madsen J,Konradsen HB

    更新日期:2005-04-01 00:00:00

  • Age-related prevalence and distribution of nontuberculous mycobacterial species among patients with cystic fibrosis.

    abstract::We studied the prevalence and species distribution of nontuberculous mycobacteria (NTM) in relation to age in 385 patients with cystic fibrosis (CF) (mean age +/- standard deviation [range], 12.0 +/- 6.1 [1 to 24] years; sex ratio, 0.53) attending three Parisian centers. The overall prevalence of NTM in sputum was 8.1...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.7.3467-3470.2005

    authors: Pierre-Audigier C,Ferroni A,Sermet-Gaudelus I,Le Bourgeois M,Offredo C,Vu-Thien H,Fauroux B,Mariani P,Munck A,Bingen E,Guillemot D,Quesne G,Vincent V,Berche P,Gaillard JL

    更新日期:2005-07-01 00:00:00

  • Rapid detection of the Marek's disease viral genome in chicken feathers by loop-mediated isothermal amplification.

    abstract::A loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek's disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.05408-11

    authors: Angamuthu R,Baskaran S,Gopal DR,Devarajan J,Kathaperumal K

    更新日期:2012-03-01 00:00:00

  • Recognition of a new serogroup of Legionnaires disease bacterium.

    abstract::A strain of the Legionnaires disease bacterium (LDB) that was isolated by Joseph E. McDade from a postmortem lung specimen of a patient with fatal atypical pneumonia at the Veterans Administration Hospital in Togus, Maine was serologically different from 16 other strains of LDB that had been isolated previously from p...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: McKinney RM,Thomason BM,Harris PP,Thacker L,Lewallen KR,Wilkinson HW,Hebert GA,Moss CW

    更新日期:1979-01-01 00:00:00

  • Clinical impact of rapid in vitro susceptibility testing and bacterial identification.

    abstract::During the past decade, a variety of instrument-assisted bacterial identification and antimicrobial susceptibility test systems have been developed which permit provision of test results in a matter of hours rather than days, as has been the case with traditional overnight procedures. These newer rapid techniques are ...

    journal_title:Journal of clinical microbiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1128/JCM.32.7.1757-1762.1994

    authors: Doern GV,Vautour R,Gaudet M,Levy B

    更新日期:1994-07-01 00:00:00

  • Evaluation of a novel dry latex preparation for demonstration of infectious mononucleosis heterophile antibody in comparison with three established tests.

    abstract::A new, dried antigen-coated latex preparation for the demonstration of infectious mononucleosis (IM) heterophile antibody (Dryspot IM kit; Oxoid, Ltd., Basingstoke, Hampshire, United Kingdom) was compared with the IM kit (a liquid latex reagent from the same source), an immunoassay (ImmunoCard Mono; Meridian Diagnosti...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.1.95-98.1999

    authors: Rogers R,Windust A,Gregory J

    更新日期:1999-01-01 00:00:00

  • Characterization of a Brucella sp. strain as a marine-mammal type despite isolation from a patient with spinal osteomyelitis in New Zealand.

    abstract::Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in a study describing infections of two patients from Peru. We report the isolation and characterization of a strain of Brucella from a New Zealand patient that appears most closely related to str...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00680-06

    authors: McDonald WL,Jamaludin R,Mackereth G,Hansen M,Humphrey S,Short P,Taylor T,Swingler J,Dawson CE,Whatmore AM,Stubberfield E,Perrett LL,Simmons G

    更新日期:2006-12-01 00:00:00

  • Infections acquired in clinical laboratories in Utah.

    abstract::We reviewed laboratory-acquired infections occurring in Utah from 1978 through 1982. Written and telephone interviews of supervisors of 1,191 laboratorians revealed an estimated annual incidence of 3 laboratory-acquired infections per 1,000 employees. Infections, in order of frequency, included hepatitis B (clinical c...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.21.4.486-489.1985

    authors: Jacobson JT,Orlob RB,Clayton JL

    更新日期:1985-04-01 00:00:00

  • Comparison of Four Serological Methods and Two Reverse Transcription-PCR Assays for Diagnosis and Surveillance of Zika Virus Infection.

    abstract::Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain-Barré syndrome in adults. Diagnosis and surv...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01785-17

    authors: Balmaseda A,Zambrana JV,Collado D,García N,Saborío S,Elizondo D,Mercado JC,Gonzalez K,Cerpas C,Nuñez A,Corti D,Waggoner JJ,Kuan G,Burger-Calderon R,Harris E

    更新日期:2018-02-22 00:00:00

  • Molecular phylogenetic diversity of dermatologic and other human pathogenic fusarial isolates from hospitals in northern and central Italy.

    abstract::Fifty-eight fusaria isolated from 50 Italian patients between 2004 and 2007 were subject to multilocus DNA sequence typing to characterize the spectrum of species and circulating sequence types (STs) associated with dermatological infections, especially onychomycoses and paronychia, and other fusarioses in northern an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01765-09

    authors: Migheli Q,Balmas V,Harak H,Sanna S,Scherm B,Aoki T,O'Donnell K

    更新日期:2010-04-01 00:00:00

  • Evaluation of PNA-FISH yeast traffic light for rapid identification of yeast directly from positive blood cultures and assessment of clinical impact.

    abstract::The PNA-FISH Yeast Traffic Light assay was performed on 54 clinical isolates of yeasts inoculated into blood culture bottles. The assay showed high sensitivity (Candida albicans/C. parapsilosis, 100%; C. glabrata/C. krusei, 92.3%; C. tropicalis, 100%) and specificity (C. albicans/C. parapsilosis, 100%; C. glabrata/C. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00028-13

    authors: Stone NR,Gorton RL,Barker K,Ramnarain P,Kibbler CC

    更新日期:2013-04-01 00:00:00

  • A clonal subpopulation of Leptospira interrogans sensu stricto is the major cause of leptospirosis outbreaks in Brazil.

    abstract::Leptospira is a highly diverse genus comprising many species and serogroups in Brazil as well as all over the world. However, a study by arbitrarily primed PCR of 44 leptospiral strains isolated from humans during three different outbreaks in Brazilian urban centers reveals that 43 of 44 isolates exhibit very similar ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Pereira MM,Matsuo MG,Bauab AR,Vasconcelos SA,Moraes ZM,Baranton G,Saint Girons I

    更新日期:2000-01-01 00:00:00

  • Detection of Legionella pneumophila capsular-like envelope antigens by counterimmunoelectrophoresis.

    abstract::The capsular-like envelope of Legionella pneumophila strains Togus 1 (serotype 2) and Philadelphia 1 (serotype 1) was isolated and purified by column chromatography on Sepharose 6B. Antibody raised in rabbits to these two antigenic materials did not cross-react in gel diffusion. Upon electrophoresis followed by gel di...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.13.4.637-642.1981

    authors: Smith RA,DiGiorgio S,Darner J,Wilhelm A

    更新日期:1981-04-01 00:00:00

  • Outbreak of nosocomial infections due to extended-spectrum beta-lactamase-producing strains of enteric group 137, a new member of the family Enterobacteriaceae closely related to Citrobacter farmeri and Citrobacter amalonaticus.

    abstract::A member of the Enterobacteriaceae initially identified as Kluyvera cryocrescens by the MicroScan Gram-Negative Combo 13 panel caused an outbreak of nosocomial infections in four patients (pneumonia, n = 2; urinary tract infection, n = 1; wound infection, n = 1) and urinary tract colonization in one patient. When the ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.11.3946-3952.2000

    authors: Warren JR,Farmer JJ 3rd,Dewhirst FE,Birkhead K,Zembower T,Peterson LR,Sims L,Bhattacharya M

    更新日期:2000-11-01 00:00:00

  • Detection of Leptospira DNA in patients with aseptic meningitis by PCR.

    abstract::Samples of cerebrospinal fluid from 103 patients with aseptic meningitis were tested by PCR for detection of leptospires, and the results were compared with those of the microscopic agglutination test (MAT) and an enzyme-linked immunosorbent assay for detection of immunoglobulin M (ELISA-IgM). Of these samples, 39.80%...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.5.1453-1455.1998

    authors: Romero EC,Billerbeck AE,Lando VS,Camargo ED,Souza CC,Yasuda PH

    更新日期:1998-05-01 00:00:00

  • Primary invasive cutaneous Microsporum canis infections in immunocompromised patients.

    abstract::Two cases of primary invasive cutaneous infections caused by the zoophilic dermatophytic species Microsporum canis are presented. The first case occurred in a liver transplant recipient who was receiving immunosuppressive therapy. Multiple erythematous papules were seen on both legs, and a biopsy revealed invasive fun...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.34.2.460-462.1996

    authors: King D,Cheever LW,Hood A,Horn TD,Rinaldi MG,Merz WG

    更新日期:1996-02-01 00:00:00

  • Detection of antibodies to caprine arthritis-encephalitis virus by protein G enzyme-linked immunosorbent assay and immunoblotting.

    abstract::Sera from goats suffering from caprine arthritis-encephalitis contained antibodies to virus proteins of 15, 17, 28, 40, and 130 kilodaltons in immunoblots of maedi-visna virus. We propose to use immunoblotting as a validation test for enzyme-linked immunosorbent assay and demonstrate that the specificity of indirect e...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.3.580-582.1989

    authors: Zanoni R,Krieg A,Peterhans E

    更新日期:1989-03-01 00:00:00

  • Evaluation of commercial enzyme-linked immunosorbent assay kits for detection of tuberculosis in Argentinean population.

    abstract::Pathozyme-Myco G (Myco G), M, A, and TB complex plus (Omega Diagnostics Ltd., Alloa, Scotland) were evaluated for the serological diagnosis of pulmonary tuberculosis (TB) in an Argentinean population. Sera from 58 patients with pulmonary TB, 24 subjects with pulmonary mycobacteriosis or mycoses (pulmonary MM group), a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.2.884-887.2004

    authors: Imaz MS,Comini MA,Zerbini E,Sequeira MD,Latini O,Claus JD,Singh M

    更新日期:2004-02-01 00:00:00

  • Enzyme-linked immunosorbent assay for detection of measles antibody.

    abstract::An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of measles immunoglobulin G antibody (MEASELISA). This assay was found to be comparable to the measles hemagglutination inhibition (HAI) test. Approximately 500 sera from three centers were tested by MEASELISA and the HAI test. MEASELISA demo...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.17.5.814-818.1983

    authors: Boteler WL,Luipersbeck PM,Fuccillo DA,O'Beirne AJ

    更新日期:1983-05-01 00:00:00

  • Use of subtractive hybridization to identify a diagnostic probe for a cystic fibrosis epidemic strain of Pseudomonas aeruginosa.

    abstract::A multiresistant strain of Pseudomonas aeruginosa is widespread among cystic fibrosis (CF) patients attending clinics in Liverpool, United Kingdom. Suppression subtractive hybridization was used to identify sequences present in the Liverpool CF epidemic strain but absent from strain PAO1. Using dot blot and PCR amplif...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.12.4607-4611.2002

    authors: Parsons YN,Panagea S,Smart CH,Walshaw MJ,Hart CA,Winstanley C

    更新日期:2002-12-01 00:00:00

  • Time-resolved fluorometry PCR assay for rapid detection of herpes simplex virus in cerebrospinal fluid.

    abstract::We have introduced a time-resolved fluorometry (TRF)-based microwell hybridization assay for PCR products in detection of herpes simplex virus (HSV) in cerebrospinal fluid (CSF) specimens. TRF is a sensitive nonradioactive detection technique which involves the use of lanthanide chelates as fluorescent labels. We used...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.9.3214-3218.2000

    authors: Hukkanen V,Rehn T,Kajander R,Sjöroos M,Waris M

    更新日期:2000-09-01 00:00:00

  • Enteric adenoviruses: detection, replication, and significance.

    abstract::Adenoviruses can be demonstrated readily in the stools of pediatric gastroenteritis patients by electron microscopy or counterimmunoelectrophoresis, but in 45% of these cases the virus will not grow in cell culture. Indirect immunofluorescence microscopy can be used to detect nongrowing strains of adenovirus; these st...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.10.4.574-578.1979

    authors: Retter M,Middleton PJ,Tam JS,Petric M

    更新日期:1979-10-01 00:00:00

  • Comparison of the Mycoplasma Duo test with PCR for detection of ureaplasma species in endotracheal aspirates from premature infants.

    abstract::We compared the Mycoplasma Duo kit (Sanofi Diagnostics Pasteur) with PCR for detection of Ureaplasma spp. in endotracheal aspirates from 60 premature neonates. The overall agreement between the two tests was 96%. The Mycoplasma Duo assay is a useful alternative to culture and PCR for detection of neonatal Ureaplasma i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.1.509-510.2005

    authors: Cheah FC,Anderson TP,Darlow BA,Murdoch DR

    更新日期:2005-01-01 00:00:00

  • Recovery of Blastomyces dermatitidis from blood of a patient with disseminated blastomycosis.

    abstract::This report describes the first well-documented reported case of the recovery of Blastomyces dermatitidis from the blood of a patient with disseminated blastomycosis. The isolate was recovered by using a lysis-centrifugation method and inhibitory mold agar after 6 days of incubation at 30 degrees C. One of six blood s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.8.1421-1423.1987

    authors: Musial CE,Wilson WR,Sinkeldam IR,Roberts GD

    更新日期:1987-08-01 00:00:00