Novel PCR-restriction fragment length polymorphism method for determining serotypes or serogroups of Streptococcus pneumoniae isolates.

abstract：:Nocardia brasiliensis, the second most frequently isolated aerobic actinomycete in the clinical laboratory, is usually associated with localized cutaneous infections. However, 22% of 238 N. brasiliensis isolates from the United States and 12% of 66 isolates from Queensland, Australia, which had been collected over a 1...

journal_title：Journal of clinical microbiology

authors： Wallace RJ Jr,Brown BA,Blacklock Z,Ulrich R,Jost K,Brown JM,McNeil MM,Onyi G,Steingrube VA,Gibson J

更新日期：1995-06-01 00:00:00

abstract：:We compared the use of bacteriophage sensitivity, seroagglutination with polyclonal antisera raised in rabbits or horses, seroagglutination with murine monoclonal antibody, and the serum agar precipitin technique for the detection of K1 capsular polysaccharide among clinical isolates of Escherichia coli obtained from ...

journal_title：Journal of clinical microbiology

authors： Cross A,Orskov I,Orskov F,Sadoff J,Gemski P

更新日期：1984-08-01 00:00:00

abstract：:In Mycobacterium smegmatis and a limited number of Mycobacterium tuberculosis strains, the involvement of alterations of the 16S rRNA gene (rrs) in resistance to kanamycin has been shown. To investigate the extent to which mutations in a specific region of the rrs gene and the kanamycin-resistant phenotype in clinical...

journal_title：Journal of clinical microbiology

authors： Suzuki Y,Katsukawa C,Tamaru A,Abe C,Makino M,Mizuguchi Y,Taniguchi H

更新日期：1998-05-01 00:00:00

abstract：:The rapid ID32A kit (bioMérieux Vitek, Inc., Hazelwood, Mo.) was evaluated for its ability to identify Peptostreptococcus species compared with conventional biochemical tests and gas-liquid chromatography (Virginia Polytechnic Institute), the current "gold standard" method. A total of 5 Peptostreptococcus American Typ...

journal_title：Journal of clinical microbiology

authors： Ng J,Ng LK,Chow AW,Dillon JA

更新日期：1994-05-01 00:00:00

abstract：:Prostate tissues from patients with prostate cancer and benign prostatic hyperplasia (BPH) frequently contain histological inflammation, and a proportion of these patients show evidence of Propionibacterium acnes infection in the prostate gland. We developed a multicolor fluorescent in situ hybridization (FISH) assay ...

journal_title：Journal of clinical microbiology

authors： Alexeyev OA,Marklund I,Shannon B,Golovleva I,Olsson J,Andersson C,Eriksson I,Cohen R,Elgh F

更新日期：2007-11-01 00:00:00

abstract：:We developed a blood spot test for syphilis antibody using enzyme-linked immunosorbent assay (ELISA) technology. Dried blood was eluted by buffered saline or, for a supplementary confirmatory test, by treponemal-antibody test diluent. Eluates were diluted in an absorption buffer (Calypte Biomedical, Berkeley, Calif.) ...

journal_title：Journal of clinical microbiology

authors： Stevens R,Pass K,Fuller S,Wiznia A,Noble L,Duva S,Neal M

更新日期：1992-09-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody response. Variability of the gG-1 gene among wild-type strains may be a factor of importance for a reliable serodiagnosis and typing of HSV-1 isolates. Here, we used a gG-1 type-spec...

journal_title：Journal of clinical microbiology

authors： Rekabdar E,Tunbäck P,Liljeqvist JA,Lindh M,Bergström T

更新日期：2002-09-01 00:00:00

abstract：:Metronidazole and tetracycline E tests were compared to an agar dilution method for the antimicrobial susceptibility testing of Helicobacter pylori. Sixteen strains were tested by using tetrazolium egg yolk (TEY) agar. The characteristic E test inhibition ellipse was clearer on TEY agar than on standard blood agar and...

journal_title：Journal of clinical microbiology

authors： Valdez Y,Velapatiño B,Gilman RH,Gutierrez V,León C

更新日期：1998-09-01 00:00:00

abstract：:We studied the plasmid profiles of 136 Shigella isolates in Bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. Many different plasmid patterns were observed within each species, indicating that many genetically different strains of Shigella are re...

journal_title：Journal of clinical microbiology

authors： Tacket CO,Shahid N,Huq MI,Alim AR,Cohen ML

更新日期：1984-08-01 00:00:00

abstract：:A multiplex PCR (mPCR) protocol was developed for simultaneous detection of the gyrB gene in Streptococcus pneumoniae, Streptococcus mitis, and Streptococcus oralis, and the specificity was evaluated using 141 coccus strains. Genomic DNAs purified from S. pneumoniae, S. mitis, and S. oralis strains were efficiently de...

journal_title：Journal of clinical microbiology

authors： Kim W,Park HK,Hwang WJ,Shin HS

更新日期：2013-03-01 00:00:00

abstract：:Current studies indicate that a significant percentage of healthy blood donors carry Chlamydia pneumoniae in their blood. Although the clinical significance of such findings is unknown, eradication of such bacteria from blood components may contribute to transfusion safety. Deletion of C. pneumoniae in Red Blood Cell ...

journal_title：Journal of clinical microbiology

authors： Ikejima H,Friedman H,Leparc GF,Yamamoto Y

更新日期：2005-09-01 00:00:00

abstract：:Whole-genome sequencing (WGS) of Staphylococcus aureus is increasingly used as part of infection prevention practices. In this study, we established a long-read technology-based WGS screening program of all first-episode methicillin-resistant Staphylococcus aureus (MRSA) blood infections at a major urban hospital. A s...

journal_title：Journal of clinical microbiology

authors： Sullivan MJ,Altman DR,Chacko KI,Ciferri B,Webster E,Pak TR,Deikus G,Lewis-Sandari M,Khan Z,Beckford C,Rendo A,Samaroo F,Sebra R,Karam-Howlin R,Dingle T,Hamula C,Bashir A,Schadt E,Patel G,Wallach F,Kasarskis A,Gi

更新日期：2019-11-22 00:00:00

abstract：:Stool specimens from 124 international travelers with acute diarrhea were tested for the presence of enteropathogens. Noroviruses (NoVs) were the second most commonly identified enteric pathogen in diarrheal stool samples (21/124, 17%), exceeded only by enterotoxigenic Escherichia coli (50/106, 47%). This study indica...

journal_title：Journal of clinical microbiology

authors： Ko G,Garcia C,Jiang ZD,Okhuysen PC,Belkind-Gerson J,Glass RI,DuPont HL

更新日期：2005-12-01 00:00:00

abstract：:Nearly 400,000 people worldwide are known to have been infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) beginning in December 2019. The virus has now spread to over 168 countries including the United States, where the first cluster of cases was observed in the Seattle metropolitan area in Was...

journal_title：Journal of clinical microbiology

authors： Nalla AK,Casto AM,Huang MW,Perchetti GA,Sampoleo R,Shrestha L,Wei Y,Zhu H,Jerome KR,Greninger AL

更新日期：2020-05-26 00:00:00

abstract：:An evaluation was undertaken to determine the optimal method for testing the susceptibilities of 100 clinical isolates and two reference strains of Enterococcus spp. to vancomycin in vitro. Six testing methods were studied by using the following media and incubation times: agar screen with the Synergy Quad Plate (Reme...

journal_title：Journal of clinical microbiology

authors： Kohner PC,Patel R,Uhl JR,Garin KM,Hopkins MK,Wegener LT,Cockerill FR 3rd

更新日期：1997-12-01 00:00:00

abstract：:PCR-restriction fragment length polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the species level. A total of 50 mycobacterial reference strains and 3 related bacterial strains were used to amplify the 360-bp region of rpoB, and ...

journal_title：Journal of clinical microbiology

authors： Lee H,Park HJ,Cho SN,Bai GH,Kim SJ

更新日期：2000-08-01 00:00:00

abstract：:The aims of this study were to compare a novel PCR-based Chlamydia trachomatis detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available C. trachomatis detection Hybrid Capture 2 (HC2) assay and to investigate the C. trachomatis serovar distribution among young women in a rural Costa Rican s...

journal_title：Journal of clinical microbiology

authors： Quint K,Porras C,Safaeian M,González P,Hildesheim A,Quint W,van Doorn LJ,Silva S,Melchers W,Schiffman M,Rodríguez AC,Wacholder S,Freer E,Cortes B,Herrero R,Costa Rican Vaccine Trial Group.

更新日期：2007-12-01 00:00:00

abstract：:To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-a...

journal_title：Journal of clinical microbiology

authors： Landry ML,Ferguson D

更新日期：2014-08-01 00:00:00

abstract：:The NCCLS proposed standard M38-P describes standard parameters for testing the fungistatic antifungal activities (MICs) of established agents against filamentous fungi (molds); however, standard conditions are not available for testing their fungicidal activities (minimum fungicidal or lethal concentrations [MFCs]). ...

journal_title：Journal of clinical microbiology

authors： Espinel-Ingroff A

更新日期：2001-03-01 00:00:00

abstract：:During an investigation of a hospital outbreak of norovirus gastroenteritis identified as being caused by a recombinant genogroup II (rGII-3a) strain, fecal specimens collected from asymptomatic staff and patients were tested by nested PCR. A GII-4 norovirus strain, the predominant strain associated with outbreaks in ...

journal_title：Journal of clinical microbiology

authors： Gallimore CI,Cubitt D,du Plessis N,Gray JJ

更新日期：2004-05-01 00:00:00

abstract：:By using the computer-assisted Dendron system to analyze the patterns of Southern blots probed with the repetitive sequence Ca3, we have compared oral isolates of Candida spp. from a group of 11 nonhospitalized patients with AIDS suffering from recurrent episodes of oral thrush in Leicester, England, with oral isolate...

journal_title：Journal of clinical microbiology

authors： Schmid J,Odds FC,Wiselka MJ,Nicholson KG,Soll DR

更新日期：1992-04-01 00:00:00

abstract：:An isolate of Proteus mirabilis recovered from blood cultures of a diabetic patient was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole-cell and/or plasmid DNA recovered from the isolate with primers specific for the bla(KPC) carbapenemase ge...

journal_title：Journal of clinical microbiology

authors： Tibbetts R,Frye JG,Marschall J,Warren D,Dunne W

更新日期：2008-09-01 00:00:00

abstract：:We evaluated the use of mannitol salt agar with oxacillin for use as a primary screening medium for the simultaneous detection and identification of methicillin-resistant Staphylococcus aureus in clinical surveillance specimens. Oxacillin agar dilution susceptibility tests with mannitol salt agar and Mueller-Hinton ag...

journal_title：Journal of clinical microbiology

authors： Lally RT,Ederer MN,Woolfrey BF

更新日期：1985-10-01 00:00:00

abstract：:HIV-1 non-B subtypes/circulating recombinant forms (CRFs) are increasing worldwide. Since subtype identification can be clinically relevant, we assessed the added value in HIV-1 subtyping using updated molecular phylogeny (Mphy) and the performance of routinely used automated tools. Updated Mphy (2015 updated referenc...

journal_title：Journal of clinical microbiology

authors： Fabeni L,Berno G,Fokam J,Bertoli A,Alteri C,Gori C,Forbici F,Takou D,Vergori A,Zaccarelli M,Maffongelli G,Borghi V,Latini A,Pennica A,Mastroianni CM,Montella F,Mussini C,Andreoni M,Antinori A,Perno CF,Santoro MM,

更新日期：2017-09-01 00:00:00

abstract：:The bacterial genus Gordona includes seven species of mycolic acid-containing actinomycetes which are cultured from environmental sources and rarely from clinical samples. They have been implicated in primary pulmonary diseases, nosocomial wound infections, and central nervous system infections in two immunocompromise...

journal_title：Journal of clinical microbiology

authors： Drancourt M,Pelletier J,Cherif AA,Raoult D

更新日期：1997-02-01 00:00:00

abstract：:Despite the high virological response rates achieved with current directly acting antiviral agents (DAAs) against hepatitis C virus (HCV), around 2% to 5% of treated patients do not achieve a sustained viral response. The identification of amino acid substitutions associated with treatment failure requires analytical ...

journal_title：Journal of clinical microbiology

authors： Soria ME,García-Crespo C,Martínez-González B,Vázquez-Sirvent L,Lobo-Vega R,de Ávila AI,Gallego I,Chen Q,García-Cehic D,Llorens-Revull M,Briones C,Gómez J,Ferrer-Orta C,Verdaguer N,Gregori J,Rodríguez-Frías F,Buti M,Este

更新日期：2020-11-18 00:00:00

abstract：:A procedure for extracting rotaviral double-stranded ribonucleic acid (RNA) directly from fecal and intestinal specimens collected from calves and pigs is described. This procedure provides a rapid, simple, reproducible method of obtaining rotaviral double-stranded RNA preparations suitable for electrophoretic analysi...

journal_title：Journal of clinical microbiology

authors： Theil KW,McCloskey CM,Saif LJ,Redman DR,Bohl EH,Hancock DD,Kohler EM,Moorhead PD

更新日期：1981-09-01 00:00:00

abstract：:The Vidas Chlamydia test (CHL) is an automated enzyme-linked immunofluorescence assay for the detection of Chlamydia trachomatis. Positive and equivocal results are confirmed with a blocking assay. A mouse monoclonal antibody directed against the chlamydial lipopolysaccharides was used for the test. The CHL assay is w...

journal_title：Journal of clinical microbiology

authors： Schachter J,Jones RB,Butler RC,Rice B,Brooks D,Van der Pol B,Gray M,Moncada J

更新日期：1997-08-01 00:00:00

abstract：:The frequency of and risk factors for methicillin-resistant Staphylococcus aureus (MRSA) transmission from a MRSA index person to household contacts were assessed in this prospective study. Between January 2005 and December 2007, 62 newly diagnosed MRSA index persons (46 patients and 16 health care workers) and their ...

journal_title：Journal of clinical microbiology

authors： Mollema FP,Richardus JH,Behrendt M,Vaessen N,Lodder W,Hendriks W,Verbrugh HA,Vos MC

更新日期：2010-01-01 00:00:00

abstract：:Phenolphthalein diphosphate was incorporated into a primary blood agar medium for use in performing quantitative urine cultures. Phosphatase-negative staphylococci, such as Staphylococcus saprophyticus, were differentiated from phosphatase-positive species, such as Staphylococcus epidermidis, by spot testing colonies ...

journal_title：Journal of clinical microbiology

authors： Pickett DA,Welch DF

更新日期：1985-03-01 00:00:00