Detection of kanamycin-resistant Mycobacterium tuberculosis by identifying mutations in the 16S rRNA gene.

abstract：:The attenuated bacillus Calmette-Guérin (BCG) vaccine strain is derived from a virulent strain of Mycobacterium bovis. BCG is difficult to differentiate from other strains of M. bovis and other members of the M. tuberculosis complex by conventional methods. Recently, a genomic region designated RD1 was found to be pre...

journal_title：Journal of clinical microbiology

authors： Talbot EA,Williams DL,Frothingham R

更新日期：1997-03-01 00:00:00

abstract：:Increasing diversity among influenza H5N1 viruses has resulted in the need for sensitive and specific diagnostic assays, fully validated for the detection of H5 viruses belonging to all hemagglutinin (HA) clades, particularly the recently circulating H5N1 viruses of clade 2. In this report, the development and validat...

journal_title：Journal of clinical microbiology

authors： Ellis JS,Smith JW,Braham S,Lock M,Barlow K,Zambon MC

更新日期：2007-05-01 00:00:00

abstract：:From November 2000 to October 2001, a reverse transcription-PCR using primers directed to the norovirus RNA polymerase coding region was included in a viral and bacterial routine screening to diagnose sporadic cases of acute gastroenteritis among children in Asturias, Spain. The role of noroviruses (8.6% of the positi...

journal_title：Journal of clinical microbiology

authors： Boga JA,Melón S,Nicieza I,De Diego I,Villar M,Parra F,De Oña M

更新日期：2004-06-01 00:00:00

abstract：:Recently, we reported that the patterns of antibodies to Helicobacter pylori protein antigens in serum may be useful for screening patients at high risk for ulcers (P. Aucher et al., J. Clin. Microbiol. 36:931-936, 1998). Here we report the identification, by a combination of electrophoretic, immunochemical, and prote...

journal_title：Journal of clinical microbiology

authors： Atanassov C,Pezennec L,d'Alayer J,Grollier G,Picard B,Fauchère JL

更新日期：2002-02-01 00:00:00

abstract：:Time to detection (TTD) in automated blood culture systems is delayed for sensitive microorganisms in the presence of antimicrobial substances and has been associated with worse outcomes for sepsis patients on inadequate empirical therapy. While resin addition removes antimicrobial substances to various degrees from b...

journal_title：Journal of clinical microbiology

authors： Mitteregger D,Barousch W,Nehr M,Kundi M,Zeitlinger M,Makristathis A,Hirschl AM

更新日期：2013-05-01 00:00:00

abstract：:Infected aneurysms caused by Yersinia are very uncommon and are principally due to Yersinia enterocolitica. We describe the first case of an infected aneurysm caused by Yersinia pseudotuberculosis in an elderly patient with a history of atherosclerotic cardiovascular disease. ...

journal_title：Journal of clinical microbiology

authors： Hadou T,Elfarra M,Alauzet C,Guinet F,Lozniewski A,Lion C

更新日期：2006-09-01 00:00:00

abstract：:The GenoType MTBDRsl test rapidly detects resistance to ethambutol, fluoroquinolones, and second-line aminoglycosides (amikacin and kanamycin) and cyclic peptide (capreomycin) in Mycobacterium tuberculosis. A set of 41 multidrug-resistant (MDR) M. tuberculosis strains, 8 extensively drug-resistant (XDR) M. tuberculosi...

journal_title：Journal of clinical microbiology

authors： Brossier F,Veziris N,Aubry A,Jarlier V,Sougakoff W

更新日期：2010-05-01 00:00:00

abstract：:In a survey of bloodstream infection (BSI) isolates across the continental United States, 162 Candida albicans isolates were fingerprinted with the species-specific probe Ca3 and the patterns were analyzed for relatedness with a computer-assisted system. The results demonstrate that particular BSI strains are more hig...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Lockhart SR,Pujol C,Swails-Wenger JA,Messer SA,Edmond MB,Jones RN,Wenzel RP,Soll DR

更新日期：1998-06-01 00:00:00

abstract：:The ERA strain of rabies virus was adapted to growth on monolayers of a porcine kidney cell line (PK-2A). A reproducible plaque assay system was subsequently developed, which appears to be satisfactory for conducting plaque neutralization tests. ...

journal_title：Journal of clinical microbiology

authors： Strating A,Mitzel JR,Slater EA

更新日期：1976-08-01 00:00:00

abstract：:Potomac horse fever is an acute systemic equine disease caused by Ehrlichia risticii. Currently, serologic methods are widely used to diagnose this disease. However, serologic methods cannot determine whether the horse is presently infected or has been exposed to ehrlichial antigens in the past. The purpose of the pre...

journal_title：Journal of clinical microbiology

authors： Mott J,Rikihisa Y,Zhang Y,Reed SM,Yu CY

更新日期：1997-09-01 00:00:00

abstract：:We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, ...

journal_title：Journal of clinical microbiology

authors： Edouard S,Prudent E,Gautret P,Memish ZA,Raoult D

更新日期：2015-03-01 00:00:00

abstract：:Over a decade ago the first cryptococcal lateral flow assay (LFA) became available. This affordable point-of-care test has undeniably led to tremendous improvements in early detection of cryptococcosis (1,2). However, from several studies it must be concluded that the reliability of recently introduced LFA's falls sho...

journal_title：Journal of clinical microbiology

authors： Shi D,Haas PJ,Boekhout T,Hahn RC,Hagen F

更新日期：2021-01-20 00:00:00

abstract：:Ureaplasma urealyticum has been associated with urethritis in men, obstetric problems in women, and respiratory distress syndrome in preterm infants. U. urealyticum can be divided into two biovars comprising 14 serovars. Partial sequences of genes encoding the multiple-banded antigens of the cell surface are known. Us...

journal_title：Journal of clinical microbiology

authors： Pitcher D,Sillis M,Robertson JA

更新日期：2001-05-01 00:00:00

abstract：:Four independent molecular methods were used to characterize the Salmonella enterica subsp. enterica serovar choleraesuis live vaccine strains SC-54 and Suisaloral and to differentiate them from S. choleraesuis field isolates. Plasmid analysis revealed the presence of seven plasmid profiles. A virulence plasmid of 52-...

journal_title：Journal of clinical microbiology

authors： Weide-Botjes M,Liebisch B,Schwarz S,Watts JL

更新日期：1996-10-01 00:00:00

abstract：:A noncompetitive enzyme-linked immunosorbent assay that enables the quantitation of toxic shock syndrome toxin 1 (TSST-1) to as little as 30 pg/ml and the detection of TSST-1 to 10 pg/ml in phosphate-buffered saline including 33% human serum or plasma was developed. It takes only 3 h to complete this assay after plate...

journal_title：Journal of clinical microbiology

authors： Miwa K,Fukuyama M,Kunitomo T,Igarashi H

更新日期：1994-02-01 00:00:00

abstract：:In this study, the optimal combination of three commercial glycoprotein G-2 (gG-2)-based herpes simplex virus type 2 (HSV-2) type-specific enzyme-linked immunosorbent assays (Euroimmun anti-HSV-2 immunoglobulin G [IgG] ELISA [Eu2], Gull HSV-2-specific IgG ELISA [Gu2], and Radim HSV-2 IgG ELISA [Ra2]) and one gG-2-base...

journal_title：Journal of clinical microbiology

authors： Eing BR,Lippelt L,Lorentzen EU,Hafezi W,Schlumberger W,Steinhagen K,Kühn JE

更新日期：2002-02-01 00:00:00

abstract：:The reactivation of an uncommon type I/III recombinant-genotype Toxoplasma gondii strain resulted in unusually severe encephalitis and chorioretinitis associated with a cerebral salt wasting syndrome in an African human immunodeficiency virus patient. This observation suggests an influence of the parasite genotype on ...

journal_title：Journal of clinical microbiology

authors： Genot S,Franck J,Forel JM,Rebaudet S,Ajzenberg D,de Paula AM,Dardé ML,Stein A,Ranque S

更新日期：2007-09-01 00:00:00

abstract：:Eight strains of a new intermediate adenovirus were isolated in the course of an investigation of conjunctivitis in Hiroshima City, Japan. The strain was first isolated in July 1986. All eight strains were isolated from conjunctival swab samples from patients with conjunctivitis, mainly epidemic keratoconjunctivitis. ...

journal_title：Journal of clinical microbiology

authors： Noda M,Miyamoto Y,Ikeda Y,Matsuishi T,Ogino T

更新日期：1991-07-01 00:00:00

abstract：:The objective of the present study was the development of a diagnostic reverse transcription (RT)-PCR for the specific detection of enterovirus (EV) RNA in clinical specimens controlled by an internal control (IC) RNA. The IC RNA contains the same primer binding sites as EV RNA but has a different probe region. The IC...

journal_title：Journal of clinical microbiology

authors： Beld M,Minnaar R,Weel J,Sol C,Damen M,van der Avoort H,Wertheim-van Dillen P,van Breda A,Boom R

更新日期：2004-07-01 00:00:00

abstract：:Accreditation in Ontario, Canada, requires that licensed clinical laboratories participate in external quality assessment (also known as proficiency testing) and perform competency evaluation of their staff. To assess the extent of ongoing competency assessment practices, the Quality Management Program--Laboratory Ser...

journal_title：Journal of clinical microbiology

authors： Desjardins M,Fleming CA

更新日期：2014-08-01 00:00:00

abstract：:The performance of the Enigma MiniLab assay for influenza A and B viruses and respiratory syncytial virus (RSV) was compared to a centralized laboratory respiratory virus panel. The positive and negative percent agreement for influenza A virus, influenza B virus, and RSV were 79.2% (95% confidence interval [95% CI], 5...

journal_title：Journal of clinical microbiology

authors： Douthwaite ST,Walker C,Adams EJ,Mak C,Vecino Ortiz A,Martinez-Alier N,Goldenberg SD

更新日期：2016-01-01 00:00:00

abstract：:Optimal conditions for a fluorescence immunoassay of antibodies to, and surface antigens of, Actinomyces viscosus ATCC 19246 are described. In the standard fluorescence immunoassay, 10(8) colony-forming units of A. viscosus reacted with an antibody preparation, were washed, and then were treated with an excess of fluo...

journal_title：Journal of clinical microbiology

authors： Gillis TP,Thompson JJ

更新日期：1978-02-01 00:00:00

abstract：:Antibodies to Japanese encephalitis virus (JEV) nonstructural 1 (NS1) protein constitute a marker of natural JEV infection among populations vaccinated with inactivated JE vaccine. In Japan, with few recent human JE cases, the natural infection rate is critical to evaluate the necessity of continuing JE vaccination. A...

journal_title：Journal of clinical microbiology

authors： Konishi E,Shoda M,Ajiro N,Kondo T

更新日期：2004-11-01 00:00:00

abstract：:A variety of fungi produce the hydrolytic enzyme beta-N-acetylhexosaminidase (HexNAcase), which can be readily detected in assays by using p-nitrophenyl-N-acetyl-beta-D-glucosaminide as a substrate. In the present study we developed a microtiter plate-based HexNAcase assay for distinguishing Candida albicans and Candi...

journal_title：Journal of clinical microbiology

authors： Niimi K,Shepherd MG,Cannon RD

更新日期：2001-06-01 00:00:00

abstract：:In a prospective, multicenter study of 342 blood samples from 187 patients with systemic inflammatory response syndrome, sepsis, or neutropenic fever, a new commercial PCR test (SepsiTest; Molzym) was evaluated for rapid diagnosis of bacteremia. The test comprises a universal PCR from the 16S rRNA gene, with subsequen...

journal_title：Journal of clinical microbiology

authors： Wellinghausen N,Kochem AJ,Disqué C,Mühl H,Gebert S,Winter J,Matten J,Sakka SG

更新日期：2009-09-01 00:00:00

abstract：:Three susceptibility testing procedures were compared to determine fluconazole, itraconazole, and ketoconazole MICs against 47 Candida albicans strains isolated sequentially from the oral cavities of five AIDS patients undergoing azole therapy. They included the broth microdilution method (BM), performed according to ...

journal_title：Journal of clinical microbiology

authors： Tortorano AM,Viviani MA,Barchiesi F,Arzeni D,Rigoni AL,Cogliati M,Compagnucci P,Scalise G

更新日期：1998-06-01 00:00:00

abstract：:The aims of this study were to compare a novel PCR-based Chlamydia trachomatis detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available C. trachomatis detection Hybrid Capture 2 (HC2) assay and to investigate the C. trachomatis serovar distribution among young women in a rural Costa Rican s...

journal_title：Journal of clinical microbiology

authors： Quint K,Porras C,Safaeian M,González P,Hildesheim A,Quint W,van Doorn LJ,Silva S,Melchers W,Schiffman M,Rodríguez AC,Wacholder S,Freer E,Cortes B,Herrero R,Costa Rican Vaccine Trial Group.

更新日期：2007-12-01 00:00:00

abstract：:Nucleic acid amplification tests, such as PCR, are the method of choice for respiratory virus testing, due to their superior diagnostic accuracy and fast turnaround time. The Panther Fusion (Fusion; Hologic) system has an array of highly sensitive in vitro diagnostic (IVD) real-time PCR assays for respiratory viruses,...

journal_title：Journal of clinical microbiology

authors： Stellrecht KA,Cimino JL,Maceira VP

更新日期：2020-05-26 00:00:00

abstract：:We tested 606 specimens for cytomegalovirus infection. Fifty-two specimens were positive by conventional culture versus forty-three by pre-cytopathic effect detection. Twenty-nine specimens were positive by both methods. Because of toxicity, specimens should be set up on both conventional and shell vial culture system...

journal_title：Journal of clinical microbiology

authors： Rabella N,Drew WL

更新日期：1990-04-01 00:00:00

abstract：:Previously, we designed an internally controlled quantitative nested real-time (QNRT) PCR assay for Mycobacterium tuberculosis DNA in order to rapidly diagnose tuberculous meningitis. This technique combined the high sensitivity of nested PCR with the accurate quantification of real-time PCR. In this study, we attempt...

journal_title：Journal of clinical microbiology

authors： Takahashi T,Tamura M,Asami Y,Kitamura E,Saito K,Suzuki T,Takahashi SN,Matsumoto K,Sawada S,Yokoyama E,Takasu T

更新日期：2008-05-01 00:00:00