Diagnosis of bacteremia in whole-blood samples by use of a commercial universal 16S rRNA gene-based PCR and sequence analysis.

abstract：:Salmonella enterica serovar Senftenberg is a common nontyphoidal Salmonella serotype which causes human Salmonella infections worldwide. In this study, 182 S. Senftenberg isolates, including 17 atypical non-hydrogen sulfide (H2S)-producing isolates, were detected in China from 2005 to 2011. The microbiological and gen...

journal_title：Journal of clinical microbiology

authors： Yi S,Xie J,Liu N,Li P,Xu X,Li H,Sun J,Wang J,Liang B,Yang C,Wang X,Hao R,Wang L,Wu Z,Zhang J,Wang Y,Huang L,Sun Y,Klena JD,Meng J,Qiu S,Song H

更新日期：2014-07-01 00:00:00

abstract：:In the context of hematopoietic stem cell transplantation, adenovirus infections are associated with relevant mortality and morbidity. Detection of adenovirus DNA by quantitative PCR is the "gold standard" for these patients. A total of 150 samples, namely, 78 whole-blood, 22 cerebrospinal fluid, 24 digestive biopsy, ...

journal_title：Journal of clinical microbiology

authors： Jeulin H,Salmon A,Bordigoni P,Venard V

更新日期：2010-09-01 00:00:00

abstract：:Leishmania (Viannia) braziliensis causes three main types of American tegumentary leishmaniasis (ATL), localized cutaneous leishmaniasis (CL), mucosal leishmaniasis (ML), and disseminated leishmaniasis (DL). All forms are observed among individuals of Corte de Pedra, Brazil. We previously used random amplified markers...

journal_title：Journal of clinical microbiology

authors： Queiroz A,Sousa R,Heine C,Cardoso M,Guimarães LH,Machado PR,Carvalho EM,Riley LW,Wilson ME,Schriefer A

更新日期：2012-12-01 00:00:00

abstract：:Large hospital-based clinical laboratories must be prepared to rapidly investigate potential infectious disease outbreaks. To challenge the ability of our molecular diagnostics laboratory to use whole-genome sequencing in a potential outbreak scenario and identify impediments to these efforts, we studied 84 invasive s...

journal_title：Journal of clinical microbiology

authors： Olsen RJ,Fittipaldi N,Kachroo P,Sanson MA,Long SW,Como-Sabetti KJ,Valson C,Cantu C,Lynfield R,Van Beneden C,Beres SB,Musser JM

更新日期：2014-12-01 00:00:00

abstract：:Results of a 2003 survey carried out in Italy to evaluate the prevalence of extended-spectrum beta-lactamase (ESBL)-producing enterobacteria are presented. Eleven Italian Microbiology Laboratories investigated 9,076 consecutive nonreplicate isolates (inpatients, 6,850; outpatients, 2,226). ESBL screening was performed...

journal_title：Journal of clinical microbiology

authors： Luzzaro F,Mezzatesta M,Mugnaioli C,Perilli M,Stefani S,Amicosante G,Rossolini GM,Toniolo A

更新日期：2006-05-01 00:00:00

abstract：:The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with HindIII, EcoRV, and AspI to generate restriction fragmen...

journal_title：Journal of clinical microbiology

authors： Carter SE,Ravyn MD,Xu Y,Johnson RC

更新日期：2001-09-01 00:00:00

abstract：:The staphylococcal methicillin resistance determinant, mecA, resides on a mobile genetic element, staphylococcus chromosomal cassette mec (SCCmec). The distribution of SCCmec in nature is limited to relatively few clonal complexes of related methicillin-resistant Staphylococcus aureus (MRSA). We have previously report...

journal_title：Journal of clinical microbiology

authors： Katayama Y,Robinson DA,Enright MC,Chambers HF

更新日期：2005-05-01 00:00:00

abstract：:A simple method, using commercial disks to detect Escherichia coli mutator strains, is proposed. The breakpoint for detecting strains with a mutation frequency >/=5 x 10(-7) was established at >/=70 and >/=20 colonies in the inhibition zone of fosfomycin and rifampin disks, respectively, after seeding 100 microl of an...

journal_title：Journal of clinical microbiology

authors： Galán JC,Tato M,Baquero MR,Turrientes C,Baquero F,Martinez JL

更新日期：2004-09-01 00:00:00

abstract：:A serological study was performed to determine recent human exposure to Burkholderia pseudomallei (the cause of melioidosis) in residents of southern Thailand affected by the tsunami of 26 December 2004. The findings were suggestive of increased recent exposure in both tsunami survivors and uninjured bystanders. Survi...

journal_title：Journal of clinical microbiology

authors： Wuthiekanun V,Chierakul W,Rattanalertnavee J,Langa S,Sirodom D,Wattanawaitunechai C,Winothai W,White NJ,Day N,Peacock SJ

更新日期：2006-01-01 00:00:00

abstract：:The frequency of and risk factors for methicillin-resistant Staphylococcus aureus (MRSA) transmission from a MRSA index person to household contacts were assessed in this prospective study. Between January 2005 and December 2007, 62 newly diagnosed MRSA index persons (46 patients and 16 health care workers) and their ...

journal_title：Journal of clinical microbiology

authors： Mollema FP,Richardus JH,Behrendt M,Vaessen N,Lodder W,Hendriks W,Verbrugh HA,Vos MC

更新日期：2010-01-01 00:00:00

abstract：:Monoclonal antibodies to K88ac and K88ab were used in enzyme-linked immunosorbent assays on Escherichia coli cultures known to produce K88 pili. A total of 415 K88-positive E. coli isolates from nine states were all found to be the K88ac variant. The cultures tested were isolated during the years 1976 to 1985. ...

journal_title：Journal of clinical microbiology

authors： Westerman RB,Mills KW,Phillips RM,Fortner GW,Greenwood JM

更新日期：1988-01-01 00:00:00

abstract：:We evaluated the abilities of three different avian influenza A viruses to attenuate the wild-type human influenza A/Korea/1/82 (H3N2) virus in squirrel monkeys, chimpanzees, and adult seronegative human volunteers. Two of these, avian influenza A/Mallard/NY/78 and A/Mallard/Alberta/76 viruses, appeared to be satisfac...

journal_title：Journal of clinical microbiology

authors： Snyder MH,Clements ML,Herrington D,London WT,Tierney EL,Murphy BR

更新日期：1986-09-01 00:00:00

abstract：:Recent outbreaks of severe acute respiratory syndrome (SARS) have spurred intense research efforts around the world to deal with the serious threat to health posed by this novel coronavirus. A rapid, reliable diagnostic assay is needed for monitoring the spread of the disease. Here we report a method for eliminating f...

journal_title：Journal of clinical microbiology

authors： Hui RK,Zeng F,Chan CM,Yuen KY,Peiris JS,Leung FC

更新日期：2004-05-01 00:00:00

abstract：:A combined method of the Limulus amebocyte lysate (LAL) test and the enzyme-linked immunosorbent assay for quantitation of endotoxin was developed based on our observation that the antigenicity of coagulogen, a major protein in LAL, was lost when LAL reacted with endotoxin as shown by immunoblotting. Determination of ...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Koch C

更新日期：1988-08-01 00:00:00

abstract：:Molecular genotyping of strains of Trichophyton rubrum and T. mentagrophytes from patients with onychomycosis of the toes was performed to ascertain whether the fungal genotype changes over the course of time as sequential samples were obtained from patients receiving antifungal therapy and during follow-up. Sixty-six...

journal_title：Journal of clinical microbiology

authors： Gupta AK,Kohli Y,Summerbell RC

更新日期：2001-09-01 00:00:00

abstract：:A total of 41 Brucella isolates, examined by standard biotyping procedures, were found to be similar to Brucella abortus biovar 2 in dye sensitivity but had a dominant M antigen. Oxidative metabolic tests performed on 39 of the isolates confirmed them as B. abortus. Additional biochemical and bacteriophage susceptibil...

journal_title：Journal of clinical microbiology

authors： Ewalt DR,Forbes LB

更新日期：1987-04-01 00:00:00

abstract：:A surveillance network was implemented by the Istituto Superiore di Sanità of Rome in collaboration with laboratories of virology in Czech Republic, Slovenia, Croatia, Albania, and Bulgaria. About 1,500 rotavirus-positive stool samples were collected from children with severe gastroenteritis admitted to hospitals or o...

journal_title：Journal of clinical microbiology

authors： Tcheremenskaia O,Marucci G,De Petris S,Ruggeri FM,Dovecar D,Sternak SL,Matyasova I,Dhimolea MK,Mladenova Z,Fiore L,Rotavirus Study Group.

更新日期：2007-07-01 00:00:00

abstract：:In a large prospective comparison, the illumigene test detected Clostridium difficile in 98% of toxin-positive and 58% of toxin-negative samples confirmed positive by other methods. The Xpert was uniformly sensitive. Most samples with discrepant results had C. difficile concentrations below the illumigene limit of det...

journal_title：Journal of clinical microbiology

authors： Gyorke CE,Wang S,Leslie JL,Cohen SH,Solnick JV,Polage CR

更新日期：2013-01-01 00:00:00

abstract：:The incidence of enterovirus 71 (EV71) infection has greatly increased in the Asian Pacific region since 1997. Several large outbreaks, caused by different subgenogroups of EV71, occurred with high rates of morbidity and a substantial number of deaths. In 2007, 58 cases of EV71 infection requiring hospitalization were...

journal_title：Journal of clinical microbiology

authors： van der Sanden S,Koopmans M,Uslu G,van der Avoort H,Dutch Working Group for Clinical Virology.

更新日期：2009-09-01 00:00:00

abstract：:We report a case of staphylococcal exanthematous disease in a newborn due to a toxic shock syndrome toxin 1- and SEC-producing methicillin-resistant Staphylococcus aureus strain and alert neonatologists to the probable emergence in France of the neonatal toxic shock syndrome-like exanthematous disease in newborns prev...

journal_title：Journal of clinical microbiology

authors： van der Mee-Marquet N,Lina G,Quentin R,Yaouanc-Lapalle H,Fièvre C,Takahashi N,Etienne J

更新日期：2003-10-01 00:00:00

abstract：:A total of 2,559 routine clinical specimens were cultured for mycobacteria by using BACTEC Middlebrook 7H12 medium (BACTEC), Lowenstein-Jensen slants (LJ), and Mycobactosel selective Middlebrook 7H11 slants (M7H11). Thirty-three isolates (1.3%) of M. avium complex and 82 isolates (3.2%) of M. tuberculosis were recover...

journal_title：Journal of clinical microbiology

authors： Kirihara JM,Hillier SL,Coyle MB

更新日期：1985-11-01 00:00:00

abstract：:In 271 Enterobacter blood culture isolates from 12 hospitals, extended-spectrum beta-lactamase (ESBL) prevalence varied between 0% and 30% per hospital. High prevalence was associated with dissemination, indicating the potential relevance of infection control measures. Screening with cefepime or Vitek 2, followed by a...

journal_title：Journal of clinical microbiology

authors： Stuart JC,Diederen B,Al Naiemi N,Fluit A,Arents N,Thijsen S,Vlaminckx B,Mouton JW,Leverstein-van Hall M

更新日期：2011-07-01 00:00:00

abstract：:The nucleotide sequences of the internal transcribed spacer (ITS) 1 and 2 regions in the rRNA gene were determined by directly sequencing PCR-amplified fragments for all of the species (17 species and five varieties) in the genus Trichosporon. Comparative sequence analysis suggests that six medically relevant species,...

journal_title：Journal of clinical microbiology

authors： Sugita T,Nishikawa A,Ikeda R,Shinoda T

更新日期：1999-06-01 00:00:00

abstract：:The epidemiological study of several multidrug-resistant Enterobacteriaceae isolated from five patients demonstrated in vivo dissemination of a 100-kb plasmid encoding the extended-spectrum beta-lactamase TEM-24 from a clonal strain of Enterobacter aerogenes to different strains of Klebsiella pneumoniae, Escherichia c...

journal_title：Journal of clinical microbiology

authors： Neuwirth C,Siebor E,Pechinot A,Duez JM,Pruneaux M,Garel F,Kazmierczak A,Labia R

更新日期：2001-05-01 00:00:00

abstract：:This study compares the identification, typing, and phylogenetic relationships of the most prevalent clinical Nocardia species in Spain, as determined via sequence analysis of their housekeeping genes gyrB and rpoB, with the results returned by the gold standard 16S rRNA method. gyrB and rpoB analyses identified Nocar...

journal_title：Journal of clinical microbiology

authors： Carrasco G,Valdezate S,Garrido N,Villalón P,Medina-Pascual MJ,Sáez-Nieto JA

更新日期：2013-11-01 00:00:00

abstract：:Swine influenza viruses (SIV) have been shown to sporadically infect humans and are infrequently identified by the Influenza Division of the Centers for Disease Control and Prevention (CDC) after being received as unsubtypeable influenza A virus samples. Real-time reverse transcriptase PCR (rRT-PCR) procedures for det...

journal_title：Journal of clinical microbiology

authors： Shu B,Wu KH,Emery S,Villanueva J,Johnson R,Guthrie E,Berman L,Warnes C,Barnes N,Klimov A,Lindstrom S

更新日期：2011-07-01 00:00:00

abstract：:Of 4,000 endocervical specimens tested with the Chlamydiazyme enzyme-linked immunosorbent assay (Abbott Laboratories), 233 (5.8%) gave positive results (A492 above the cutoff), which were confirmed with a blocking reagent (Abbott). An additional 34 specimens (14.6%) with chlamydial antigen were detected and confirmed ...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Seiple JW,Stroll ES

更新日期：1993-06-01 00:00:00

abstract：:Fifteen strains of an anaerobic, catalase-negative, gram-positive diphtheroid-shaped bacterium recovered from human sources were characterized by phenotypic and molecular chemical and molecular genetic methods. The unidentified bacterium showed some resemblance to Actinomyces species and related taxa, but biochemical ...

journal_title：Journal of clinical microbiology

authors： Hall V,Collins MD,Lawson PA,Hutson RA,Falsen E,Inganas E,Duerden B

更新日期：2003-02-01 00:00:00

abstract：:A cytopathic astrovirus was isolated from pigs with acute diarrhea in an established cell line that was derived from porcine embryonic kidneys with the aid of trypsin. The virus showed a distinct cytopathic effect characterized by an enlargement of cells and the appearance of fine granules in the cytoplasm. Porcine as...

journal_title：Journal of clinical microbiology

authors： Shimizu M,Shirai J,Narita M,Yamane T

更新日期：1990-02-01 00:00:00

abstract：:Specimens of colonic mucosa from two pigs with diarrhea were examined by light and electron microscopy. The epithelial surfaces of both pigs were extensively colonized by large spirochetes morphologically compatible with Treponema hyodysenteriae or Treponema innocens. The microorganisms were intimately attached end-on...

journal_title：Journal of clinical microbiology

authors： Jacques M,Girard C,Higgins R,Goyette G

更新日期：1989-05-01 00:00:00