Cost-effective pooling of DNA from nasopharyngeal swab samples for large-scale detection of bacteria by real-time PCR.

abstract：:The emergence of a clinically daptomycin-resistant Staphylococcus aureus isolate occurred during treatment of methicillin-resistant S. aureus bacteremia and probable vertebral osteomyelitis. The breakthrough isolate was indistinguishable from pretreatment daptomycin-susceptible isolates by pulsed-field gel electrophor...

journal_title：Journal of clinical microbiology

authors： Marty FM,Yeh WW,Wennersten CB,Venkataraman L,Albano E,Alyea EP,Gold HS,Baden LR,Pillai SK

更新日期：2006-02-01 00:00:00

abstract：:Chlamydia pneumoniae is a newly recognized organism associated with respiratory tract infections. Asymptomatic infection with C. pneumoniae, although it has been suggested to occur, has not been previously documented. We describe two asymptomatic individuals infected with this organism; these infections demonstrate th...

journal_title：Journal of clinical microbiology

authors： Hyman CL,Augenbraun MH,Roblin PM,Schachter J,Hammerschlag MR

更新日期：1991-09-01 00:00:00

abstract：:Prosthetic joint infection (PJI) is a rare but refractory complication of arthroplasty. Accurate identification of pathogens is a key step for successful treatment of PJI, which remains a challenge for clinicians and laboratory workers. We designed a combined culture method with sonication of implants and incubation i...

journal_title：Journal of clinical microbiology

authors： Shen H,Tang J,Wang Q,Jiang Y,Zhang X

更新日期：2015-03-01 00:00:00

abstract：:Thirty stool filtrates known to contain Clostridium difficile toxin based on previous testing on McCoy cells were tested for toxicity on primary African green monkey kidney (AGMK), McCoy, MRC-5, primary rhesus monkey kidney (RMK), and Vero cells. All 30 filtrates showed cytotoxic effect at greater than or equal to 1:1...

journal_title：Journal of clinical microbiology

authors： Maniar AC,Williams TW,Hammond GW

更新日期：1987-10-01 00:00:00

abstract：:A PCR method was developed to identify and fingerprint Candida krusei isolates simply and rapidly. The primer pair Arno1 and Arno2 was designed to amplify the polymorphic species-specific repetitive sequence CKRS-1 (C. krusei repeated sequence 1) that we identified in the nontranscribed intergenic regions (IGRs) of rR...

journal_title：Journal of clinical microbiology

authors： Carlotti A,Chaib F,Couble A,Bourgeois N,Blanchard V,Villard J

更新日期：1997-06-01 00:00:00

abstract：:Accurate identification of pathogenic species is important for early appropriate patient management, but growing diversity of infectious species/strains makes the identification of clinical yeasts increasingly difficult. Among conventional methods that are commercially available, the API ID32C, AuxaColor, and Vitek 2 ...

journal_title：Journal of clinical microbiology

authors： Posteraro B,Efremov L,Leoncini E,Amore R,Posteraro P,Ricciardi W,Sanguinetti M

更新日期：2015-08-01 00:00:00

abstract：:Nocardia cyriacigeorgica is a recently characterized species within the genus of Nocardia. We report a brain abscess, following a primary pulmonary colonization, due to this species in a human immunodeficiency virus-infected patient. This case confirms that isolation of Nocardia in sputum is associated with a high ris...

journal_title：Journal of clinical microbiology

authors： Barnaud G,Deschamps C,Manceron V,Mortier E,Laurent F,Bert F,Boiron P,Vinceneux P,Branger C

更新日期：2005-09-01 00:00:00

abstract：:The API STAPH-IDENT system was compared with conventional methods for the identification of 14 Staphylococcus species. Conventional methods included the Kloos and Schleifer simplified scheme and DNA-DNA hybridization. The API STAPH-IDENT strip utilizes a battery of 10 miniaturized biochemical tests, including alkaline...

journal_title：Journal of clinical microbiology

authors： Kloos WE,Wolfshohl JF

更新日期：1982-09-01 00:00:00

abstract：:A rapid method for the detection of acetylneuraminyl hydrolase, EC 3.2.1.18 (sialidase or neuraminidase), was developed by using 2'-(4-methylumbelliferyl)alpha-D-N-acetylneuraminic acid as substrate in a filter paper spot test. The method was compared to conventional assays that use 2'-(4-methylumbelliferyl)alpha-D-N-...

journal_title：Journal of clinical microbiology

authors： Moncla BJ,Braham P

更新日期：1989-01-01 00:00:00

abstract：:Acute and convalescent-phase sera obtained from 10 patients infected with a Japanese strain of spotted fever group (SFG) rickettsia were tested by the indirect immunoperoxidase test, the Weil-Felix test, an enzyme-linked immunosorbent assay (ELISA), and immunoblotting. By the Weil-Felix test, the reactivity of these s...

journal_title：Journal of clinical microbiology

authors： Amano K,Hatakeyama H,Okuta M,Suto T,Mahara F

更新日期：1992-09-01 00:00:00

abstract：:In 271 Enterobacter blood culture isolates from 12 hospitals, extended-spectrum beta-lactamase (ESBL) prevalence varied between 0% and 30% per hospital. High prevalence was associated with dissemination, indicating the potential relevance of infection control measures. Screening with cefepime or Vitek 2, followed by a...

journal_title：Journal of clinical microbiology

authors： Stuart JC,Diederen B,Al Naiemi N,Fluit A,Arents N,Thijsen S,Vlaminckx B,Mouton JW,Leverstein-van Hall M

更新日期：2011-07-01 00:00:00

abstract：:Antibody responses of 85 patients to human immunodeficiency virus type 1 antigens were quantitated by densitometric analysis of Western blot (immunoblot) assays. All patients had been classified into the following three clinical categories: asymptomatic (ASY), acquired immunodeficiency syndrome (AIDS)-related complex ...

journal_title：Journal of clinical microbiology

authors： Schmidt G,Amiraian K,Frey H,Wethers J,Stevens RW,Berns DS

更新日期：1989-05-01 00:00:00

abstract：:Morganella ("Proteus") morganii is the only species in the recently proposed genus Morganella, but we suspect there are yet undescribed species in the genus. Two candidates for new species were recently investigated. Nineteen strains isolated from clinical specimens resembled M. morganii but were lysine positive and n...

journal_title：Journal of clinical microbiology

authors： Hickman FW,Framer JJ 3rd,Steigerwalt AG,Brenner DJ

更新日期：1980-07-01 00:00:00

abstract：:A rapid, sensitive counterimmunoelectrophoresis assay was developed to detect adenovirus in stools of patients with gastroenteritis. The parameters of the assay were optimized, and its sensitivity and efficiency was examined. The assay promises to be a useful alternative to conventional techniques of cell culture isol...

journal_title：Journal of clinical microbiology

authors： Mankikar SD,Middleton PJ,Petric M

更新日期：1979-08-01 00:00:00

abstract：:A recombinant protein (p41-G) of an antigenic region of flagellin was used in a standard and amplified enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Borrelia burgdorferi, the causative agent of Lyme borreliosis. Comparable sensitivities (88 to 94%) were noted when sera from 17 persons who had eryth...

journal_title：Journal of clinical microbiology

authors： Magnarelli LA,Fikrig E,Berland R,Anderson JF,Flavell RA

更新日期：1992-12-01 00:00:00

abstract：:We recently developed a mass spectrometry (MS) procedure based on the detection of a serum disaccharide (MS-DS) in patients with invasive candidiasis (IC). Here, we compare the performance of MS-DS for the diagnosis of IC, invasive aspergillosis (IA), and mucormycosis (MM) with those of commercially available antigen ...

journal_title：Journal of clinical microbiology

authors： Mery A,Sendid B,François N,Cornu M,Poissy J,Guerardel Y,Poulain D

更新日期：2016-11-01 00:00:00

abstract：:More than 4,000 clinical urine specimens were evaluated with an automated microbial detection/identification system compared to a standarized manual analysis and the routine modalities used in five peer-group laboratories. The comparison indicates that the automated system recognizes the nine groups of significant mic...

journal_title：Journal of clinical microbiology

authors： Isenberg HD,Gavan TL,Sonnenwirth A,Taylor WI,Washington JA 2nd

更新日期：1979-08-01 00:00:00

abstract：:Sequence detection by the 5' nuclease TaqMan assay uses online detection of internal fluorogenic probes in closed PCR tubes. Primers and probe were chosen from a part of the omlA gene common to all serotypes of Actinobacillus pleuropneumoniae, which gave an amplicon of 92 bp. The test was evaluated with 73 lung isolat...

journal_title：Journal of clinical microbiology

authors： Angen O,Jensen J,Lavritsen DT

更新日期：2001-01-01 00:00:00

abstract：:A serotyping scheme for Vibrio vulnificus predicated on the detection of lipopolysaccharide (LPS) antigens is proposed. The serovar O typing scheme used to type V. vulnificus employs polyclonal antisera raised in rabbits immunized with heat-killed whole-cell vaccines. Polyclonal typing sera produced in this manner cro...

journal_title：Journal of clinical microbiology

authors： Martin SJ,Siebeling RJ

更新日期：1991-08-01 00:00:00

abstract：:Sheep and guinea pigs were immunized with cellular and extracellular antigen from Legionella pneumophila bacteria. After immunization, the animals developed immunoglobulin G titers against the immunizing agent. The same sera were also tested in a Mycoplasma pneumoniae complement fixation test. All the preimmunization ...

journal_title：Journal of clinical microbiology

authors： Berdal BP,Eng J

更新日期：1982-11-01 00:00:00

abstract：:A selective medium was developed for the isolation of Bacteroides fragilis directly from ovine and bovine fecal samples. The medium (tryptose blood agar plus polymyxin B, triclosan (Irgasan), novobiocin, and nalidixic acid) permitted growth of B. fragilis and several other species of Bacteroides but inhibited growth o...

journal_title：Journal of clinical microbiology

authors： Border M,Firehammer BD,Shoop DS,Myers LL

更新日期：1985-03-01 00:00:00

abstract：:Cryptococcus neoformans and Cryptococcus gattii are the main pathogenic species of invasive cryptococcosis among the Cryptococcus species. Taxonomic studies have shown that these two taxa have different genotypes or molecular types with biological and ecoepidemiological peculiarities. Matrix-assisted laser desorption ...

journal_title：Journal of clinical microbiology

authors： Siqueira LPM,Gimenes VMF,de Freitas RS,Melhem MSC,Bonfietti LX,da Silva AR Jr,Souza Santos LB,Motta AL,Rossi F,Benard G,de Almeida JN Jr

更新日期：2019-01-02 00:00:00

abstract：:Vibrio cholerae O1 biotype El Tor (ET), the cause of the current 7th pandemic, has recently been replaced in Asia and Africa by an altered ET biotype possessing cholera toxin (CTX) of the classical (CL) biotype that originally caused the first six pandemics before becoming extinct in the 1980s. Until recently, the ET ...

journal_title：Journal of clinical microbiology

authors： Alam M,Nusrin S,Islam A,Bhuiyan NA,Rahim N,Delgado G,Morales R,Mendez JL,Navarro A,Gil AI,Watanabe H,Morita M,Nair GB,Cravioto A

更新日期：2010-10-01 00:00:00

abstract：:By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the sampl...

journal_title：Journal of clinical microbiology

authors： Osaki T,Taguchi H,Yamaguchi H,Kamiya S

更新日期：1998-01-01 00:00:00

abstract：:The accuracy of the new API YeastIdent system and the Flow Laboratories Uni-Yeast-Tek identification kit with an expanded data base was evaluated in comparison to the API 20C yeast identification system by three laboratories. A total of 489 test isolates were used, biased toward yeasts commonly encountered in clinical...

journal_title：Journal of clinical microbiology

authors： Salkin IF,Land GA,Hurd NJ,Goldson PR,McGinnis MR

更新日期：1987-04-01 00:00:00

abstract：:We report a fatal case of a chromoblastomycosis-like infection caused by a novel species of Fonsecaea in a 52-year-old immunocompetent Caucasian male from an area of chromoblastomycosis endemicity in Brazil. The patient had a 30-year history of slowly evolving, verrucous lesions on the right upper arm which gradually ...

journal_title：Journal of clinical microbiology

authors： de Azevedo CM,Gomes RR,Vicente VA,Santos DW,Marques SG,do Nascimento MM,Andrade CE,Silva RR,Queiroz-Telles F,de Hoog GS

更新日期：2015-08-01 00:00:00

abstract：:The human pathogen Staphylococcus aureus is isolated and characterized using traditional culture and sensitivity methodologies that are slow and offer limited information on the organism. In contrast, DNA microarray technology can provide detailed, clinically relevant information on the isolate by detecting the presen...

journal_title：Journal of clinical microbiology

authors： Spence RP,Wright V,Ala-Aldeen DA,Turner DP,Wooldridge KG,James R

更新日期：2008-05-01 00:00:00

abstract：:Because congenital toxoplasmosis is almost solely the result of maternal infection acquired during gestation, it is critical to determine whether infection during pregnancy has occurred. In the United States, definitive diagnosis of the acute infection and the time of its occurrence have been compromised by a lack of ...

journal_title：Journal of clinical microbiology

authors： Montoya JG,Liesenfeld O,Kinney S,Press C,Remington JS

更新日期：2002-07-01 00:00:00

abstract：:In acute and chronic hepatitis B, antibodies of the immunoglobulin M (IgM) class against the hepatitis B core antigen (anti-HBc IgM) have been demonstrated. For the determination of anti-HBc IgM, a sensitive enzyme immunoassay with anti-mu-coated flat-bottomed microtiter plates is described and evaluated. The specific...

journal_title：Journal of clinical microbiology

authors： Roggendorf M,Deinhardt F,Frösner GG,Scheid R,Bayerl B,Zachoval R

更新日期：1981-04-01 00:00:00

abstract：:In a large prospective comparison, the illumigene test detected Clostridium difficile in 98% of toxin-positive and 58% of toxin-negative samples confirmed positive by other methods. The Xpert was uniformly sensitive. Most samples with discrepant results had C. difficile concentrations below the illumigene limit of det...

journal_title：Journal of clinical microbiology

authors： Gyorke CE,Wang S,Leslie JL,Cohen SH,Solnick JV,Polage CR

更新日期：2013-01-01 00:00:00