Counterimmunoelectrophoresis assay for detection of adenovirus antigen.

abstract：:Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. Ho...

journal_title：Journal of clinical microbiology

authors： Bidet P,Lalande V,Salauze B,Burghoffer B,Avesani V,Delmée M,Rossier A,Barbut F,Petit JC

更新日期：2000-07-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody response. Variability of the gG-1 gene among wild-type strains may be a factor of importance for a reliable serodiagnosis and typing of HSV-1 isolates. Here, we used a gG-1 type-spec...

journal_title：Journal of clinical microbiology

authors： Rekabdar E,Tunbäck P,Liljeqvist JA,Lindh M,Bergström T

更新日期：2002-09-01 00:00:00

abstract：:Several problems remain before molecular biology-based techniques, such as PCR, are widely accepted for the detection of infectious agents. Among the most formidable of these problems are the inability of the tests to distinguish between viable and nonviable organisms. We approached this problem by using the fact that...

journal_title：Journal of clinical microbiology

authors： Jou NT,Yoshimori RB,Mason GR,Louie JS,Liebling MR

更新日期：1997-05-01 00:00:00

abstract：:Six Food and Drug Administration (FDA)-licensed human immunodeficiency virus type 1 (HIV-1) and HIV-1/2 immunoassays, including five enzyme immunoassays and one rapid test, were challenged with up to 250 serum samples collected from various global sites. The serum samples were from individuals known to be infected wit...

journal_title：Journal of clinical microbiology

authors： Koch WH,Sullivan PS,Roberts C,Francis K,Downing R,Mastro TD,Nkengasong J,Hu D,Masciotra S,Schable C,Lal RB

更新日期：2001-03-01 00:00:00

abstract：:In acute and chronic hepatitis B, antibodies of the immunoglobulin M (IgM) class against the hepatitis B core antigen (anti-HBc IgM) have been demonstrated. For the determination of anti-HBc IgM, a sensitive enzyme immunoassay with anti-mu-coated flat-bottomed microtiter plates is described and evaluated. The specific...

journal_title：Journal of clinical microbiology

authors： Roggendorf M,Deinhardt F,Frösner GG,Scheid R,Bayerl B,Zachoval R

更新日期：1981-04-01 00:00:00

abstract：:The L1 major capsid proteins of six human papillomavirus type 16 (HPV-16) strains were expressed in insect cells by using recombinant baculoviruses. Virus-like particles (VLPs) which appeared similar to empty virions were identified by electron microscopy for all HPV strains investigated. However, the yield of VLPs pr...

journal_title：Journal of clinical microbiology

authors： Touze A,El Mehdaoui S,Sizaret PY,Mougin C,Muñoz N,Coursaget P

更新日期：1998-07-01 00:00:00

abstract：:A total of 53 strains of enterococci, including recently isolated strains with high-level resistance to vancomycin, were tested for vancomycin susceptibility by broth microdilution and disk diffusion using Mueller-Hinton media with and without supplementation with 5% blood. By using currently published parameters of t...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Hill BC,Thornsberry C

更新日期：1989-09-01 00:00:00

abstract：:Respiratory tract infections are a leading cause of morbidity and mortality worldwide. Even with the advancement of diagnostic tools, the causative agent of 20 to 30% of upper respiratory tract infections go undiagnosed. Recently, a newly identified human respiratory virus, human metapneumovirus (hMPV), was discovered...

journal_title：Journal of clinical microbiology

authors： Regev L,Hindiyeh M,Shulman LM,Barak A,Levy V,Azar R,Shalev Y,Grossman Z,Mendelson E

更新日期：2006-04-01 00:00:00

abstract：:We report a case of a 27-year-old male who presented with respiratory distress that required mechanical ventilation. Transbronchial biopsy revealed adiaspores of the fungus Emmonsia crescens within granulomata, a condition known as adiaspiromycosis. The patient received amphotericin products and corticosteroids, follo...

journal_title：Journal of clinical microbiology

authors： Anstead GM,Sutton DA,Graybill JR

更新日期：2012-04-01 00:00:00

abstract：:The Aspergillus galactomannan test was performed on cerebrospinal fluid and serum samples from 5 patients with probable cerebral aspergillosis and from 16 control patients. Cerebrospinal fluid galactomannan levels were significantly higher in aspergillosis patients, and most galactomannan was produced intrathecally. C...

journal_title：Journal of clinical microbiology

authors： Viscoli C,Machetti M,Gazzola P,De Maria A,Paola D,Van Lint MT,Gualandi F,Truini M,Bacigalupo A

更新日期：2002-04-01 00:00:00

abstract：:Restriction fragment length polymorphism (RFLP) analysis with probes derived from the insertion element IS6110, the direct repeat sequence, and the polymorphic GC-rich sequence (PGRS) and a PCR-based typing method called spacer oligonucleotide typing (spoligotyping) were used to strain type Mycobacterium bovis isolate...

journal_title：Journal of clinical microbiology

authors： Costello E,O'Grady D,Flynn O,O'Brien R,Rogers M,Quigley F,Egan J,Griffin J

更新日期：1999-10-01 00:00:00

abstract：:Restriction endonuclease-generated polynucleotide and synthetically produced oligonucleotide gene probes used in colony hybridization assays proved to be efficient for the detection and differentiation of enterotoxigenic Escherichia coli. To compare their relative efficiencies, these two sets of probes were radiolabel...

journal_title：Journal of clinical microbiology

authors： Sommerfelt H,Kalland KH,Raj P,Moseley SL,Bhan MK,Bjorvatn B

更新日期：1988-11-01 00:00:00

abstract：:It has been shown by analysis with monoclonal and polyclonal antibodies that outer surface protein C (OspC) of Borrelia burgdorferi sensu lato is highly heterogeneous. To determine if the heterogeneity has a genetic basis, the genes of 18 different B. burgdorferi sensu lato strains have been amplified by PCR, cloned, ...

journal_title：Journal of clinical microbiology

authors： Jauris-Heipke S,Liegl G,Preac-Mursic V,Rössler D,Schwab E,Soutschek E,Will G,Wilske B

更新日期：1995-07-01 00:00:00

abstract：:A passive hemagglutination (PHA) assay for antibody to Pseudomonas aeruginosa exotoxin is described which utilizes chromic chloride-treated ovine erythrocytes coated with purified toxin. PHA antitoxin titers correlated well with those obtained by a cytotoxicity neutralization assay (r = 0.91, P less than 0.001), where...

journal_title：Journal of clinical microbiology

authors： Pollack M,Taylor NS

更新日期：1977-07-01 00:00:00

abstract：:An echovirus 3 (Echo3) strain (strain LR31G7) was isolated from a sewage treatment plant in Greece in 2005. Full-genome molecular, phylogenetic, and SimPlot analyses were conducted in order to reveal the evolutionary pathways of the isolate. Nucleotide and phylogenetic analyses of part of the VP1 genomic region reveal...

journal_title：Journal of clinical microbiology

authors： Kyriakopoulou Z,Dedepsidis E,Pliaka V,Tsakogiannis D,Pratti A,Levidiotou-Stefanou S,Markoulatos P

更新日期：2010-05-01 00:00:00

abstract：:Invasive aspergillosis (IA) is a life-threatening infection that mainly occurs in immunocompromised patients. Here, we compared the novel Aspergillus-specific galactomannoprotein (GP) enzyme-linked immunosorbent assay (ELISA) (Euroimmun Medizinische Labordiagnostika AG) to the established Platelia Aspergillus galactom...

journal_title：Journal of clinical microbiology

authors： Dichtl K,Seybold U,Ormanns S,Horns H,Wagener J

更新日期：2019-06-25 00:00:00

abstract：:Prosthetic joint infection (PJI) is a rare but refractory complication of arthroplasty. Accurate identification of pathogens is a key step for successful treatment of PJI, which remains a challenge for clinicians and laboratory workers. We designed a combined culture method with sonication of implants and incubation i...

journal_title：Journal of clinical microbiology

authors： Shen H,Tang J,Wang Q,Jiang Y,Zhang X

更新日期：2015-03-01 00:00:00

abstract：:Our results show that impairment of the gastrointestinal tracts in human immunodeficiency virus (HIV)-positive patients is present in the early phases of HIV disease. This impairment is associated with alterations in gut microbiota and intestinal inflammatory parameters. These findings support the hypothesis that alte...

journal_title：Journal of clinical microbiology

authors： Gori A,Tincati C,Rizzardini G,Torti C,Quirino T,Haarman M,Ben Amor K,van Schaik J,Vriesema A,Knol J,Marchetti G,Welling G,Clerici M

更新日期：2008-02-01 00:00:00

abstract：:As part of an ongoing investigation into nosocomial Legionella infections at Stanford University Medical Center (SUMC), we applied the technique of restriction endonuclease analysis (REA) to determine strain differences among three species, including Legionella pneumophila, Legionella dumoffii, and Legionella micdadei...

journal_title：Journal of clinical microbiology

authors： Tompkins LS,Troup NJ,Woods T,Bibb W,McKinney RM

更新日期：1987-10-01 00:00:00

abstract：:The lack of a quick, simple, and inexpensive diagnostic test has limited the ability of public health officials to rapidly assess and control outbreaks of Giardia lamblia in child day-care centers. We evaluated the performance of a commercially available enzyme-linked immunosorbent assay (ELISA) for the detection of a...

journal_title：Journal of clinical microbiology

authors： Addiss DG,Mathews HM,Stewart JM,Wahlquist SP,Williams RM,Finton RJ,Spencer HC,Juranek DD

更新日期：1991-06-01 00:00:00

abstract：:A new anti-human immunodeficiency virus type 1 and 2 (anti-HIV 1 and 2) test is described. It uses recombinant p24 and peptides covering gp32, gp41, and gp120 to identify HIV-1 and HIV-2 infections. This test has been shown to be specific (99.5%) and sensitive (99.8%). In this respect, the assay was equal or superior ...

journal_title：Journal of clinical microbiology

authors： Hess G,Avillez F,Lourenco MH,D'Agostino F,Cambie G,Piot P,Vercauteren G,Michl U,Melchior W,Bayer H

更新日期：1994-02-01 00:00:00

abstract：:The Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis, (AP), and 20 age- and sex-matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen ...

journal_title：Journal of clinical microbiology

authors： Wittstock M,Flemmig TF,Schmidt H,Mutters R,Karch H

更新日期：1996-10-01 00:00:00

abstract：:A rapid method for detection of respiratory syncytial virus (RSV) in nasopharyngeal aspirates, involving a combination of reverse transcription and polymerase chain reaction amplification (RT-PCR), has been developed. The RT-PCR assay employs oligonucleotide primers specific for the region of the RSV genome which enco...

journal_title：Journal of clinical microbiology

authors： Paton AW,Paton JC,Lawrence AJ,Goldwater PN,Harris RJ

更新日期：1992-04-01 00:00:00

abstract：:The human metapneumovirus (hMPV) was recently identified and linked to acute respiratory tract infections (ARTI). To assess the clinical importance of this virus in infants and children, we developed a rapid and efficient reverse transcription-PCR-based screening method for a large volume of samples and tested retrosp...

journal_title：Journal of clinical microbiology

authors： Chano F,Rousseau C,Laferrière C,Couillard M,Charest H

更新日期：2005-11-01 00:00:00

abstract：:A 42-plex clustered regularly interspaced short palindromic repeat (CRISPR)-based typing technique (spoligotyping) was recently developed at the French National Reference Center for Legionella. It allows the subtyping of the Legionella pneumophila sequence type 1/Paris pulsotype. In this report, we present the transfe...

journal_title：Journal of clinical microbiology

authors： Gomgnimbou MK,Ginevra C,Peron-Cane C,Versapuech M,Refrégier G,Jacotin N,Sola C,Jarraud S

更新日期：2014-07-01 00:00:00

abstract：:Assessment of cure of Trypanosoma cruzi infection by antibody seroconversion usually involves several years of follow-up. Parasitological negativity is useless for cure assessment, since even untreated patients mostly show negative results; conversely, positive tests are of great value because they indicate treatment ...

journal_title：Journal of clinical microbiology

authors： Galvão LM,Chiari E,Macedo AM,Luquetti AO,Silva SA,Andrade AL

更新日期：2003-11-01 00:00:00

abstract：:A fully automated microparticle enzyme immunoassay (EIA), IMx Core, was developed for the detection of antibody against hepatitis B core antigen (anti-HBc). IMx Core sensitivity was less than 0.5 Paul Ehrlich Institut units per ml and was greater than that of the commercial radioimmunoassay (RIA) or EIA, Corab and Cor...

journal_title：Journal of clinical microbiology

authors： Spronk AM,Schmidt L,Krenc C,Pavlis-Jenkins L,Brady J,Taskar S,Angus-Finn L,Mimms L

更新日期：1991-03-01 00:00:00

abstract：:We examined the reliability and accuracy of a colorimetric assay using Alamar Blue reagent in the performance of susceptibility tests for Candida albicans. We compared the broth macrodilution method recommended by the National Committee for Clinical Laboratory Standards (NCCLS) with a macrodilution method modified wit...

journal_title：Journal of clinical microbiology

authors： Tiballi RN,He X,Zarins LT,Revankar SG,Kauffman CA

更新日期：1995-04-01 00:00:00

abstract：:To evaluate the accuracy and utility of the Eiken Systek No. 1 (Eiken system; Eiken Chemical Co., Ltd., Tokyo, Japan), we conducted a clinical comparison, with 345 Enterobacteriaceae isolates, of the Eiken System with API 20E (Analytab Products, Inc., Plainview, N.Y.) and conventional methods. The Eiken system is a 21...

journal_title：Journal of clinical microbiology

authors： Castillo CB,Bruckner DA

更新日期：1984-10-01 00:00:00

abstract：:Tubercle bacilli may survive in unstained heat-fixed sputum smears and may be an infection risk to laboratory staff. We compared the effectiveness of 1% and 5% sodium hypochlorite, 5% phenol, 2% glutaraldehyde, and 3.7% formalin in killing Mycobacterium tuberculosis present in smears prepared from 51 sputum samples. T...

journal_title：Journal of clinical microbiology

authors： Giacomelli LR,Helbel C,Ogassawara RL,Barreto AM,Martins FM,Cardoso CL,Leite CQ

更新日期：2005-08-01 00:00:00