Comparative evaluation of the Eiken and API 20E systems and conventional methods for identification of members of the family Enterobacteriaceae.

abstract：:Maternal prenatal screening for group B streptococci (GBS) followed by offering of intrapartum chemoprophylaxis to carriers is one of the strategies used to reduce the incidence of neonatal early-onset GBS infections. Culturing of vaginal and anorectal swab specimens in selective broth is the screening procedure recom...

journal_title：Journal of clinical microbiology

authors： Hansen SM,Sørensen UB

更新日期：2003-04-01 00:00:00

abstract：:Salmonella typhi was isolated from 369 and Salmonella paratyphi A was isolated from 6 of 515 Vietnamese patients with suspected enteric fever. Compared with conventional broth culture of blood, direct plating of the buffy coat had a diagnostic sensitivity of 99.5% (95% confidence interval [CI], 97.1 to 100%). Blood ba...

journal_title：Journal of clinical microbiology

authors： Wain J,Diep TS,Ho VA,Walsh AM,Nguyen TT,Parry CM,White NJ

更新日期：1998-06-01 00:00:00

abstract：:Asymptomatic Epstein-Barr virus (EBV) reactivations periodically occur in oral mucosa-associated lymphoid tissues. Until now, EBV reactivation has been diagnosed by serologic profiles that suggest virus replication. Serologic responses, however, are delayed and do not necessarily indicate ongoing replicative activity....

journal_title：Journal of clinical microbiology

authors： Maurmann S,Fricke L,Wagner HJ,Schlenke P,Hennig H,Steinhoff J,Jabs WJ

更新日期：2003-12-01 00:00:00

abstract：:A rapid method for presumptive identification of black-pigmented gram-negative anaerobic rods was developed. Using filter paper spot tests for indole production, sialidase, alpha-glucosidase, beta-glucosidase, alpha-fucosidase, and trypsinlike enzyme activities, 100% of Porphyromonas gingivalis, Prevotella intermedia,...

journal_title：Journal of clinical microbiology

authors： Moncla BJ,Braham P,Rabe LK,Hillier SL

更新日期：1991-09-01 00:00:00

abstract：:The role of atypical enteropathogenic Escherichia coli (EPEC) in childhood diarrhea is controversial. The aim of the present study was to search for genes linked with diarrhea in atypical EPEC strains from a case-control study among Norwegian children. Using DNA microarray analysis, genomic DNAs from strains isolated ...

journal_title：Journal of clinical microbiology

authors： Afset JE,Bruant G,Brousseau R,Harel J,Anderssen E,Bevanger L,Bergh K

更新日期：2006-10-01 00:00:00

abstract：:False sensitivities were occasionally encountered in antibiotic-free chambers of the Autobac I system. This unusual phenomenon occurred randomly among gram-negative bacilli and appears to be colistin-related. ...

journal_title：Journal of clinical microbiology

authors： Duckenfield L,Wadke M,Ashton JK

更新日期：1980-10-01 00:00:00

abstract：:In a prospective volume-controlled study, the sensitivity of the BACTEC MYCO/F LYTIC medium was similar to that of the Peds Plus/F and Plus Aerobic/F vials for detecting Brucella melitensis bacteremia, but the time to detection with the MYCO/F LYTIC medium (101.4 +/- 46.7 h) was significantly longer than that with the...

journal_title：Journal of clinical microbiology

authors： Yagupsky P

更新日期：2004-05-01 00:00:00

abstract：:Timely diagnosis of respiratory syncytial virus (RSV) infection is critical for appropriate treatment of lower respiratory infection in young children. To facilitate diagnosis, we developed a rapid, specific, and sensitive TaqMan PCR method for detection of RSV A and RSV B. Two sets of primer-probe pairs were selected...

journal_title：Journal of clinical microbiology

authors： Hu A,Colella M,Tam JS,Rappaport R,Cheng SM

更新日期：2003-01-01 00:00:00

abstract：:Blood culture records from 1994 to 1995 from five U.S. medical centers all using the Difco ESP continuous monitoring blood culture system were reviewed retrospectively. Among a total of 7,362 isolates of bacteria and yeasts, only 0.1% of possibly significant isolates would have been missed had blood cultures been rout...

journal_title：Journal of clinical microbiology

authors： Doern GV,Brueggemann AB,Dunne WM,Jenkins SG,Halstead DC,McLaughlin JC

更新日期：1997-05-01 00:00:00

abstract：:The region surrounding mecA in methicillin-resistant Staphylococcus aureus (MRSA) is highly variable. We describe an approach for the rapid genotyping of MRSA by assaying for the presence or absence of variable or mobile elements previously shown to be associated with the mecA region. ...

journal_title：Journal of clinical microbiology

authors： Huygens F,Nimmo GR,Schooneveldt J,Munckhof WJ,Giffard PM

更新日期：2002-08-01 00:00:00

abstract：:We evaluated the clinical performance of the FilmArray blood culture identification (BCID) panel in the identification of microorganisms from positive blood culture bottles inoculated with sterile body fluids. All organisms included in the FA BCID panel were accurately identified in 84/84 (100%) and 18/24 (75%) sample...

journal_title：Journal of clinical microbiology

authors： Altun O,Almuhayawi M,Ullberg M,Özenci V

更新日期：2015-02-01 00:00:00

abstract：:Test strips for the detection of antibodies to human immunodeficiency virus type 1 were investigated using specimens from risk groups in Thailand (141 reactive; 445 nonreactive) in a local Thai laboratory. The diagnostic sensitivity and specificity were both 100%. Using a set of seroconversion panels, the sensitivity ...

journal_title：Journal of clinical microbiology

authors： Pagcharoenpol P,Burgess-Cassler A,Schramm W

更新日期：1996-04-01 00:00:00

abstract：:The value of performing routine aerobic subcultures of both vented and unvented blood culture bottles has not been evaluated critically. We studied 4,954 pairs of blood culture bottles consisting of one vented biphasic tryptic soy broth bottle (Roche Diagnostics) and one unvented Thiol broth bottle (Difco Laboratories...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Westfall LM,Murray PR

更新日期：1983-04-01 00:00:00

abstract：:Reagent-impregnated filter paper replica methods are described which can be used to detect all penicillinase (beta-lactamase)-producing Neisseria gonorrhoeae (PPNG) colonies in an entire population of Neisseria gonorrhoeae growing on primary isolation plates. ...

journal_title：Journal of clinical microbiology

authors： Bae BH,Ledesma G,Korzis J

更新日期：1983-03-01 00:00:00

abstract：:HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific f...

journal_title：Journal of clinical microbiology

authors： Estrade C,Sahli R

更新日期：2014-11-01 00:00:00

abstract：:There is a lack of consensus among investigators who use a variety of immunoassay techniques (e.g., enzyme-linked immunosorbent assay [ELISA] and radioimmunoassay) regarding the protocols for describing and forming standard reference or calibration curves and interpolating serum antibody concentrations. This confounds...

journal_title：Journal of clinical microbiology

authors： Plikaytis BD,Holder PF,Pais LB,Maslanka SE,Gheesling LL,Carlone GM

更新日期：1994-10-01 00:00:00

abstract：:Totals of 102 and 56 Clostridium difficile type 078 strains of human and porcine origins, respectively, from four European countries were investigated by an optimized multilocus variable-number tandem-repeat analysis (MLVA) and for tetracycline susceptibility. Eighty-five percent of all isolates were genetically relat...

journal_title：Journal of clinical microbiology

authors： Bakker D,Corver J,Harmanus C,Goorhuis A,Keessen EC,Fawley WN,Wilcox MH,Kuijper EJ

更新日期：2010-10-01 00:00:00

abstract：:Rotaviruses were detected by counterimmunoelectro-osmophoresis in the feces of 16 (13.8%) of 116 infants and young children with gastroenteritis during a 5-month period (September 1979 through January 1980) in Ife, Nigeria. The rate of rotavirus detection varied inversely with relative humidity and was highest in Dece...

journal_title：Journal of clinical microbiology

authors： Paul MO,Erinle EA

更新日期：1982-02-01 00:00:00

abstract：:Johne's disease (JD) is an economically important infectious disease in livestock farming caused by Mycobacterium avium subsp. paratuberculosis As an alternative to serological tests, which are used mainly for the screening of whole herds, we developed a novel ResoLight-based real-time PCR (RL-PCR) assay with pooled f...

journal_title：Journal of clinical microbiology

authors： Kawaji S,Nagata R,Minegishi Y,Saruyama Y,Mita A,Kishizuka S,Saito M,Mori Y

更新日期：2020-11-18 00:00:00

abstract：:mariPOC is a novel point-of-care test system for rapid detection of respiratory tract infections. We compared the performance of the mariPOC test to that of bacterial culture for detecting group A streptococcus (GAS) in 219 pharyngitis patients (ages 1-64 years) and 109 healthy asymptomatic controls (ages 19-69 years)...

journal_title：Journal of clinical microbiology

authors： Vakkila J,Koskinen JO,Brandt A,Muotiala A,Liukko V,Soittu S,Meriluoto S,Vesalainen M,Huovinen P,Irjala K

更新日期：2015-07-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and conventional standard methods were compared for time to pathogen identification and impact on clinical outcomes in peritoneal dialysis-related peritonitis patients. The MALDI-TOF MS method identified the causative microorga...

journal_title：Journal of clinical microbiology

authors： Lin WH,Hwang JC,Tseng CC,Chang YT,Wu AB,Yan JJ,Wu JJ,Wang MC

更新日期：2016-05-01 00:00:00

abstract：:Specimens from skin lesions were examined simultaneously for herpes simplex virus (HSV) and varicella-zoster virus (VZV) by direct specimen testing and shell vial culture in single-test systems. For direct testing, cells in a single specimen well were stained with a combination direct-indirect immunofluorescence stain...

journal_title：Journal of clinical microbiology

authors： Brumback BG,Farthing PG,Castellino SN

更新日期：1993-12-01 00:00:00

abstract：:Confirmative diagnosis of visceral leishmaniasis (VL) is still a challenge at the primary health care facilities in most of the rural areas of endemicity in the Indian subcontinent. Conventional methods for parasitological confirmation are risky and require skilled personnel, and hence they are unavailable to the poor...

journal_title：Journal of clinical microbiology

authors： Salam MA,Khan MG,Bhaskar KR,Afrad MH,Huda MM,Mondal D

更新日期：2012-03-01 00:00:00

abstract：:A new disposable plastic tube device and technique for ovum and parasite concentration, with upper and lower chambers separated by a fine screen, was compared with the standard gauze and open-funnel filtration technique as well as with the technique of direct smearing with an acid-fast stain. The new concentrator was ...

journal_title：Journal of clinical microbiology

authors： Zierdt WS

更新日期：1984-11-01 00:00:00

abstract：:Gemella morbillorum and Gemella haemolysans are opportunistic pathogens which cause endocarditis and other severe infections. We report on three patients with endocarditis, one with endocarditis caused by G. haemolysans and two with endocarditis caused by G. morbillorum. The paucity of reports concerning these bacteri...

journal_title：Journal of clinical microbiology

authors： La Scola B,Raoult D

更新日期：1998-04-01 00:00:00

abstract：:A Congo red-magnesium oxalate agar medium was developed to detect expression of virulence-associated calcium dependency and Congo red absorption in Yersinia enterocolitica. Of the 157 pathogenic serotypes tested, 119 (75.8%) were positive; 98% of nonpathogenic serotypes and strains of three other Yersinia species were...

journal_title：Journal of clinical microbiology

authors： Riley G,Toma S

更新日期：1989-01-01 00:00:00

abstract：:Drug-resistant tuberculosis (TB) is a major threat to TB control worldwide. Globally, only 40% of the 340,000 notified TB patients estimated to have multidrug-resistant-TB (MDR-TB) were detected in 2015. This study was carried out to evaluate the utility of high-resolution melt curve analysis (HRM) for the rapid and d...

journal_title：Journal of clinical microbiology

authors： Anthwal D,Gupta RK,Bhalla M,Bhatnagar S,Tyagi JS,Haldar S

更新日期：2017-06-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been suggested as a reliable method for bacterial identification from cultures. Direct analysis of clinical samples might increase the usefulness of this method, shortening the time for microorganism identification. We comp...

journal_title：Journal of clinical microbiology

authors： Ferreira L,Sánchez-Juanes F,González-Avila M,Cembrero-Fuciños D,Herrero-Hernández A,González-Buitrago JM,Muñoz-Bellido JL

更新日期：2010-06-01 00:00:00

abstract：:Invasive aspergillosis (IA) is a life-threatening infection that mainly occurs in immunocompromised patients. Here, we compared the novel Aspergillus-specific galactomannoprotein (GP) enzyme-linked immunosorbent assay (ELISA) (Euroimmun Medizinische Labordiagnostika AG) to the established Platelia Aspergillus galactom...

journal_title：Journal of clinical microbiology

authors： Dichtl K,Seybold U,Ormanns S,Horns H,Wagener J

更新日期：2019-06-25 00:00:00

abstract：:Broad-range amplification of bacterial DNA from clinical specimens has proved useful for the diagnosis of various bacterial infections, especially during antimicrobial treatment of the patient. Optimal sample processing protocols for diagnostic broad-range bacterial PCR should release DNA from an array of target organ...

journal_title：Journal of clinical microbiology

authors： Rantakokko-Jalava K,Jalava J

更新日期：2002-11-01 00:00:00